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#33631106   2021/02/17 To Up

Alternative RNA structures formed during transcription depend on elongation rate and modify RNA processing.

Most RNA processing occurs co-transcriptionally. We interrogated nascent pol II transcripts by chemical and enzymatic probing and determined how the "nascent RNA structureome" relates to splicing, A-I editing and transcription speed. RNA folding within introns and steep structural transitions at splice sites are associated with efficient co-transcriptional splicing. A slow pol II mutant elicits extensive remodeling into more folded conformations with increased A-I editing. Introns that become more structured at their 3' splice sites get co-transcriptionally excised more efficiently. Slow pol II altered folding of intronic Alu elements where cryptic splicing and intron retention are stimulated, an outcome mimicked by UV, which decelerates transcription. Slow transcription also remodeled RNA folding around alternative exons in distinct ways that predict whether skipping or inclusion is favored, even though it occurs post-transcriptionally. Hence, co-transcriptional RNA folding modulates post-transcriptional alternative splicing. In summary, the plasticity of nascent transcripts has widespread effects on RNA processing.
Tassa Saldi, Kent Riemondy, Benjamin Erickson, David L Bentley

1388 related Products with: Alternative RNA structures formed during transcription depend on elongation rate and modify RNA processing.

10 nmol250ul100 μg500ng1000 units10 nmol1 Bottle/Unit100 μg500ng1000 units10 nmol

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#33614527   2021/02/04 To Up

HIV-1 Productively Infects and Integrates in Bronchial Epithelial Cells.

The role of lung epithelial cells in HIV-1-related lung comorbidities remains unclear, and the major hurdle in curing HIV is the persistence of latent HIV reservoirs in people living with HIV (PLWH). The advent of combined antiretroviral therapy has considerably increased the life span; however, the incidence of chronic lung diseases is significantly higher among PLWH. Lung epithelial cells orchestrate the respiratory immune responses and whether these cells are productively infected by HIV-1 is debatable.
Dinesh Devadoss, Shashi P Singh, Arpan Acharya, Kieu Chinh Do, Palsamy Periyasamy, Marko Manevski, Neerad Mishra, Carmen S Tellez, Sundaram Ramakrishnan, Steven A Belinsky, Siddappa N Byrareddy, Shilpa Buch, Hitendra S Chand, Mohan Sopori

1830 related Products with: HIV-1 Productively Infects and Integrates in Bronchial Epithelial Cells.

1x10e7 cells1x10e7 cells1x10e7 cells1x10e7 cells1x10e7 cells100ug100ug Lyophilized1000 TESTS/0.65ml100ug Lyophilized10mg100ug1.00 flask

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#33614232   2021/01/16 To Up

Engineering highly efficient backsplicing and translation of synthetic circRNAs.

Circular RNAs (circRNAs) are highly stable RNA molecules that are attractive templates for expression of therapeutic proteins and non-coding RNAs. In eukaryotes, circRNAs are primarily generated by the spliceosome through backsplicing. Here, we interrogate different molecular elements including intron type and length, repeats, internal ribosome entry sites (IRESs), and exon length essential for circRNA formation and exploit this information to engineer robust backsplicing and circRNA expression. Specifically, we leverage the finding that the downstream intron can tolerate large inserts without affecting splicing to achieve tandem expression of backspliced circRNAs and tRNA intronic circRNAs from the same template. Further, truncation of selected intronic regions markedly increased circRNA formation in different cell types as well as AAV-mediated circRNA expression in cardiac and skeletal muscle tissue . We also observed that different IRES elements and exon length influenced circRNA expression and translation, revealing an exonic contribution to splicing, as evidenced by different RNA species produced. Taken together, these data provide new insight into improving the design and expression of synthetic circRNAs. When combined with AAV capsid and promoter technologies, the backsplicing introns and IRES elements constituting this modular platform significantly expand the gene expression toolkit.
Rita M Meganck, Jiacheng Liu, Andrew E Hale, Katherine E Simon, Marco M Fanous, Heather A Vincent, Jeremy E Wilusz, Nathaniel J Moorman, William F Marzluff, Aravind Asokan

2745 related Products with: Engineering highly efficient backsplicing and translation of synthetic circRNAs.

200ug4 Membranes/Box2 Pieces/Box100 µg10 mg1000 assays4 Arrays/Slide2 Pieces/Box10reactions 4 Membranes/Box2 Pieces/Box

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#33607070   2021/02/16 To Up

Identification of the first Alu-mediated gross deletion involving the BCKDHA gene in a compound heterozygous patient with maple syrup urine disease.

To investigate a family with clinical symptoms of maple syrup urine disease and reveal a genetic cause underlying this disease.
Shujun Ma, Zhongxin Zhang, Yanyan Fu, Mingxia Zhang, Yuna Niu, Ruiguang Li, Qinghe Guo, Zhian He, Qingwei Zhao, Zhishan Song, Xia Wang, Ruili Sun

2677 related Products with: Identification of the first Alu-mediated gross deletion involving the BCKDHA gene in a compound heterozygous patient with maple syrup urine disease.

11 mg96 tests

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#33601343   2021/02/18 To Up

Brain sources' activity in resting state before a visuo-motor task.

Objective In modern neuroscience, the underlying mechanisms of the elaboration and reaction to different kinds of stimuli of the brain hemispheres remain still very challenging to understand, together with the possibility to anticipate certain behaviors to improve the performance. Approach The purpose of the present study was to investigate the brain rhythms characteristics of EEG recordings and in particular, their interhemispheric differences in resting state condition before a visuo-motor task in a population of healthy adults. During the task, subjects were asked to react to a sequence of visual cues as quick as possible. The reaction times (RTs) to the task were measured, collected and correlated with the EEG signals recorded in a resting state condition immediately preceding the task. The EEG data were analyzed in the space of cortical sources of EEG rhythms by the computation of the Global Spectra Power Density (GSPD) in the left and in the right hemisphere, and of an index of brain Laterality L. Main results The results showed a negative correlation between the RTs and the GSPD in the central areas in the left and in the right hemisphere in both eyes open and eyes closed conditions. A close to significant and negative correlation was found in the parietal areas. Furthermore, RTs negatively correlated with L in the central areas in eyes closed condition. The results showed a negative correlation between the RTs and the GSPD in the central areas in the left and in the right hemisphere in both eyes open and eyes closed conditions. Significance The correlations between the brain activity before a task and the RTs to the task can represent an interesting tool for exploring the brain state characterization for the upcoming tasks performance.
Francesca Miraglia, Fabrizio Vecchio, Francesca Alù, Alessandro Orticoni, Elda Judica, Maria Cotelli, Paolo Maria Rossini

2913 related Products with: Brain sources' activity in resting state before a visuo-motor task.

100 μg96 tests96 assays 48 assays1 kit

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#33601025   2021/02/15 To Up

Diagnostic Role of cell free DNA Integrity in Thyroid Cancer Particularly for Bethesda IV Cytology.

Cell-free DNA integrity (cfDI) has a promising role in the differentiation between malignant and benign tumors, but little data were reported in thyroid cancer (TC). This study aimed to explore its diagnostic role in TC mainly Bethesda IV.
Aliaa M Higazi, Sahar H El Hini, Esmat A El-Sharkawy, Mariana F Gayyed, Noha A Aziz, Ragaa A Matta

2396 related Products with: Diagnostic Role of cell free DNA Integrity in Thyroid Cancer Particularly for Bethesda IV Cytology.



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#33593078   // To Up

Safety of Intraovarian Injection of Human Mesenchymal Stem Cells in a Premature Ovarian Insufficiency Mouse Model.

Primary ovarian insufficiency (POI), a condition in which there is a loss of ovarian function before the age of 40 years, leads to amenorrhea and infertility. In our previously published studies, we demonstrated recovery of POI, correction of serum sex hormone levels, increase in the granulosa cell population, and restoration of fertility in a chemotherapy-induced POI mouse model after intraovarian transplantation of human bone marrow-derived mesenchymal stem cells (hBM-MSCs). While hBM-MSC may be a promising cell source for treatment of POI, there are few reports on the safety of stem cell-based therapy for POI. For future clinical applications, the safety of allogenic hBM-MSCs for the treatment of POI through intraovarian engraftment needs to be addressed and verified in appropriate preclinical models. In this study, we induced POI in C57/BL6 mice using chemotherapy, then treated the mice with hBM-MSCs (500,000 cells/ovary) by intraovarian injection. After hBM-MSC treatment, we analyzed the migration of engrafted cells by genomic DNA polymerase chain reaction (PCR) using a human-specific ALU repeat and by whole-body sectioning on a cryo-imaging system. We examined the possibility of transfer of human DNA from the hBM-MSCs to the resulting offspring, and compared the growth rate of offspring to that of normal mice and hBM-MSC-treated mice. We found that engrafted hBM-MSCs were detected only in mouse ovaries and did not migrate into any other major organs including the heart, lungs, and liver. Further, we found that no human DNA was transferred into the fetus. Interestingly, the engrafted cells gradually decreased in number and had mostly disappeared after 4 weeks. Our study demonstrates that intraovarian transplantation of hBM-MSCs could be a safe stem cell-based therapy to restore fertility in POI patients.
Hang-Soo Park, Rishi Man Chugh, Amro Elsharoud, Mara Ulin, Sahar Esfandyari, Alshimaa Aboalsoud, Lale Bakir, Ayman Al-Hendy

1811 related Products with: Safety of Intraovarian Injection of Human Mesenchymal Stem Cells in a Premature Ovarian Insufficiency Mouse Model.

100.00 ug500 0.5 ml25 25 100 μg100ug Lyophilized100 μg100 μg100 μg1 mg200

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#33565322   2021/02/10 To Up

Concentration and integrity indexes of urine cell-free DNA as promising biomarkers for early lung cancer diagnosis.

To explore the role of urine cell-free DNA (ucfDNA) concentration and integrity indexes as potential biomarkers for lung cancer diagnosis. Quantitative real-time PCR targeting Arthrobacter luteus () repeats at three size fragments (-60, 115 and 247 bp) was performed in 55 lung cancer patients and 35 healthy individuals. ucfDNA concentration and integrity indexes were significantly higher in lung cancer patients than in healthy controls. The area under the receiver operating characteristic curve for differentiating patients with stage I/II from healthy controls by fragments concentration were 0.856, 0.909 and 0.932, respectively. In addition, the ucfDNA integrity indexes in patients with lymph node metastasis were significantly higher than in patients with non-metastatic. ucfDNA concentration and integrity indexes could serve as promising biomarkers for lung cancer diagnosis.
Sai Ren, Xiaodong Ren, Haiqin Guo, Lan Liang, Kun Wei, Lifang Guo, Xuemei Qu, Xiaotian Dai, Qing Huang

2876 related Products with: Concentration and integrity indexes of urine cell-free DNA as promising biomarkers for early lung cancer diagnosis.

100Tests1 kit(96 Wells)1 kit500 assays

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#33561426   2021/01/12 To Up

Anti-Inflammatory Effectiveness of Oral Dexamethasone 4 mg on Mandibular Third Molar Surgeries: A Split-Mouth Randomized Clinical Trial.

This study aimed to evaluate the anti-inflammatory effect of oral dexamethasone 4 mg in a single dose preemptively administered to reduce pain, swelling, and trismus following mandibular third molar surgeries.
Eulália Mendes de Oliveira, Victor Bento Oliveira, Lana Karine Araújo, Timóteo Sousa Lopes, Rodrigo Otavio Rego, Marcelo Bonifácio da Silva Sampieri

2737 related Products with: Anti-Inflammatory Effectiveness of Oral Dexamethasone 4 mg on Mandibular Third Molar Surgeries: A Split-Mouth Randomized Clinical Trial.

100 μg100ug100.00 ug1 ml100ug Lyophilized

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