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#35036319   2022/01/05 To Up

Isolated Cranial Nerve VI Palsy and Neurosyphilis: A Case Report and Review of Related Literature.

An isolated cranial nerve VI palsy is a rare initial manifestation of undiagnosed neurosyphilis. A 33-year-old male presented with a one month history of progressive headache and diplopia. Neurologic examination only revealed an isolated abducens palsy on the left. Cranial imaging was unremarkable. Examination of his cerebrospinal fluid revealed lymphocytic predominant leukocytosis and elevated protein. Microbiologic work-up were all negative. Further work-up revealed the patient to be serum Rapid Plasma Reagin and Enzyme Immunoassay reactive. Enzyme-linked immunosorbent assay for Human Immunodeficiency Virus also tested positive. His cerebrospinal fluid was then sent for Rapid Plasma Reagin to confirm the diagnosis of neurosyphilis. He completed 14 days of intravenous penicillin and was eventually discharged with partial resolution of the abducens palsy. We describe the second case of neurosyphilis presenting only with an isolated cranial nerve VI involvement. On further review, ours was the first case documented on an individual who had an undiagnosed Human Immunodeficiency Virus infection. There are various differentials for an isolated cranial neuritis but infectious causes, particularly neurosyphilis, should be considered among young individuals with known risk factors despite their apparently benign medical history.
Jao Jarro B Garcia, Jalea L Coralde, Marjorie Anne C Bagnas, Kathleen Joy O Khu

2988 related Products with: Isolated Cranial Nerve VI Palsy and Neurosyphilis: A Case Report and Review of Related Literature.

1 mg100ul1,000 tests200 25 ml Ready-to-use 100 mg100ug1000 tests500 MG

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#35034091   2022/01/16 To Up

Appraisal of vitreous syphilis antibody as a novel biomarker for the diagnosis of syphilitic uveitis: a prospective case-control study.

To determine the sensitivity and specificity of syphilis antibody tests in vitreous samples and to propose an algorithm using vitreous syphilis antibody as a supplementary test to confirm syphilitic uveitis (SU).
Sukhum Silpa-Archa, Tararat Hoopholerb, Charles Stephen Foster

1867 related Products with: Appraisal of vitreous syphilis antibody as a novel biomarker for the diagnosis of syphilitic uveitis: a prospective case-control study.

100ug100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ul

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#35007575   2022/01/07 To Up

Isotopic evidence for nitrate sources and controls on denitrification in groundwater beneath an irrigated agricultural district.

The application of N fertilisers to enhance crop yield is common throughout the world. Many crops have historically been, or are still, fertilised with N in excess of the crop requirements. A portion of the excess N is transported into underlying aquifers in the form of NO, which is potentially discharged to surface waters. Denitrification can reduce the severity of NO export from groundwater. We sought to understand the occurrence and hydrogeochemical controls on denitrification in NO-rich aquifers beneath the Emerald Irrigation Area (EIA), Queensland, Australia, a region of extensive cotton and cereal production. Multiple stable isotope (in HO, NO, DIC, DOC and SO) and radioactive isotope (H and Cl) tracers were used to develop a conceptual N process model. Fertiliser-derived N is likely incorporated, and retained in, the soil organic N pool prior to its mineralisation, nitrification, and migration into aquifers. This process, alongside the near absence of other anthropogenic N sources, results in a homogenised groundwater NO isotopic signature that allows for denitrification trends to be distinguished. Regional-scale denitrification manifests as groundwater becomes increasingly anaerobic during flow from the upgradient basalt aquifer to the downgradient alluvial aquifer. Dilution and denitrification occurs in localised electron donor-rich suboxic hyporheic zones beneath leaking irrigation channels. Using approximated isotope enrichment factors, estimates of regional-scale NO removal ranges from 22 to 93% (average: 63%), and from 57 to 91% (average: 79%) beneath leaking irrigation channels. In the predominantly oxic upgradient basalt aquifer, raised groundwater tables create pathways for NO to be transported to adjacent surface waters. In the alluvial aquifer, the transfer of NO is limited both physically (through groundwater-surface water disconnection) and chemically (through denitrification). These observations underscore the need to understand regional- and local-scale hydrogeological processes when assessing the impacts of groundwater NO on adjacent and end of system ecosystems.
Stephen J Harris, Dioni I Cendón, Stuart I Hankin, Mark A Peterson, Shuang Xiao, Bryce F J Kelly

1544 related Products with: Isotopic evidence for nitrate sources and controls on denitrification in groundwater beneath an irrigated agricultural district.

100ug100 μg900 tests100 μg1 Set100 μg1 Set4 Arrays/Slide1 Set100ug1 mg1 Set

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#35007356   2022/01/10 To Up

Validation of an indirect in-house ELISA using synthetic peptides to detect antibodies anti-gp90 and gp45 of the Equine Infectious Anemia Virus (EIAV).

Equine infectious anaemia (EIA) is controlled by identification of seropositive animals. The official diagnostic method is agar gel immunodiffusion (AGID) test, which detects antibodies against a viral core protein (p26). Although AGID is inexpensive and specific, report of results takes considerable time and the test has low analytical sensitivity.
Romina C Rusi, L Garcia, María S Cámara, Adriana R Soutullo

1067 related Products with: Validation of an indirect in-house ELISA using synthetic peptides to detect antibodies anti-gp90 and gp45 of the Equine Infectious Anemia Virus (EIAV).

100 μg100ug Lyophilized0.1 mg1001 mL100 50 1 mg1 mg1 mg

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#35001025   2022/01/06 To Up

Balloons for Kids: Anatomic Candidacy and Optimal Catheter Size for Pediatric Resuscitative Endovascular Balloon Occlusion of the Aorta (REBOA).

Resuscitative endovascular balloon occlusion of the aorta (REBOA) is a potential adjunct in pediatric trauma patients with non-compressible truncal and pelvic hemorrhage, however there are little data evaluating the anatomic considerations of REBOA in children. We evaluated the vascular dimensions and anatomic limitations of utilizing REBOA in children.
Alicia G Sykes, William B Sisson, Lucas J Wang, Matthew J Martin, Hariharan Thangarajah, John Naheedy, Nathanial Fernandez, Meghan E Nelles, Romeo C Ignacio

1299 related Products with: Balloons for Kids: Anatomic Candidacy and Optimal Catheter Size for Pediatric Resuscitative Endovascular Balloon Occlusion of the Aorta (REBOA).

100 mg 500 ml 100 mg0.2 mg1,000 tests250 mg96 Tests10 200 mg2000 rxn

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#34996078   // To Up

Correlation of ANA Characteristics with pANCA IFA Interference.

Antineutrophil cytoplasmic antibody (ANCA) testing by the indirect immunofluorescence assay (IFA) is important for the diagnosis of autoimmune vasculitis. A common analytical interference for ANCA-IFA is the presence of an antinuclear antibody (ANA), which can cause an apparent perinuclear ANCA (pANCA) result on ethanol-fixed neutrophils. Here, the association of ANA patterns, titers, and concentrations with pANCA interference is investigated.
Nicholas E Larkey, Ashley M Denome, Melissa R Snyder

1640 related Products with: Correlation of ANA Characteristics with pANCA IFA Interference.

1 module10 mg1 module1 module1 module100ug100ug11 module1 mg2.5 mg1 module

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#34987388   2021/12/20 To Up

Investigating Factors of False-Positive Results of Galactomannan Assay: A Case-Control Study in Intensive Care Units.

Studies on false-positive galactomannan (GM) enzyme immunoassay (EIA) results and treatment for critically ill patients are scarce. The study aimed to determine the false-positive rate of GM-EIA and to probe the risk factors of false positivity among patients in the intensive care units (ICUs). A case-control approach was conducted to review adult patients who had at least one GM-EIA result and were admitted to the ICU. Those who had no fungal culture were excluded. The clinical characteristics and critical care between patients with false-positive and true-negative GM index (GMI) were compared. Of 206 patients enrolled and with GM-EIA results, 20 (9.7%) were considered to have false-positive antigenemia, including 9 in bronchoalveolar lavages (BAL) and 11 in serum. A total of 148 (71.8%) were true-negatives. After paired grouping of 1:4, factors researched in the previous studies showed no significant difference. However, compared with the true-negatives, patients with positive GM test results but were incompatible with the diagnosis of invasive aspergillosis were more prone to the risk of false positivity due to the use of colistin inhalation. It seemed to be the only factor that significantly increased the risk of false positivity after multivariate analysis (adjusted odds ratio, 35.68; 95% CI, 3.77-337.51, = 0.002). Colistin inhalation treatment may contribute to false-positive GM-EIA results. The positive GMI among patients receiving colistin nebulization should be interpreted with caution.
Yu-Hsuan Hung, Hui-Hsiung Lai, Hui-Chuan Lin, Kuo-Shao Sun, Chung-Yu Chen

2623 related Products with: Investigating Factors of False-Positive Results of Galactomannan Assay: A Case-Control Study in Intensive Care Units.

4/120 Packing /sleeve/bo0.1ml (1mg/ml)

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#34985331   2022/01/05 To Up

SARS Antibody Testing in Children: Development of Oral Fluid Assays for IgG Measurements.

Seroepidemiological studies to monitor antibody kinetics are important for assessing the extent and spread of SARS-CoV-2 in a population. Noninvasive sampling methods are advantageous for reducing the need for venipuncture, which may be a barrier to investigations, particularly in pediatric populations. Oral fluids are obtained by gingiva-crevicular sampling from children and adults and are very well accepted. Enzyme immunoassays (EIAs) based on these samples have acceptable sensitivity and specificity compared to conventional serum-based antibody EIAs and are suitable for population-based surveillance. We describe the development and evaluation of SARS-CoV-2 IgG EIAs using SARS-CoV-2 viral nucleoprotein (NP) and spike (S) proteins in IgG isotype capture format and an indirect receptor-binding-domain (RBD) IgG EIA, intended for use in children as a primary endpoint. All three assays were assessed using a panel of 1,999 paired serum and oral fluids from children and adults participating in school SARS-CoV-2 surveillance studies during and after the first and second pandemic wave in the United Kingdom. The anti-NP IgG capture assay was the best candidate, with an overall sensitivity of 75% (95% confidence interval [CI]: 71 to 79%) and specificity of 99% (95% CI: 78 to 99%) compared with paired serum antibodies. Sensitivity observed in children (80%, 95% CI: 71 to 88%) was higher than that in adults (67%, CI: 60% to 74%). Oral fluid assays (OF) using spike protein and RBD antigens were also 99% specific and achieved reasonable but lower sensitivity in the target population (78%, 95% CI [68% to 86%] and 53%, 95% CI [43% to 64%], respectively). We report on the first large-scale assessment of the suitability of oral fluids for detection of SARS-CoV-2 antibody obtained from healthy children attending school. The sample type (gingiva-crevicular fluid, which is a transudate of blood but is not saliva) can be self collected. Although detection of antibodies in oral fluids is less sensitive than that in blood, our study suggests an optimal format for operational use. The laboratory methods we have developed can reliably measure antibodies in children, who are able to take their own samples. Our findings are of immediate practical relevance for use in large-scale seroprevalence studies designed to measure exposure to infection, as they typically require venipuncture. Overall, our data indicate that OF assays based on the detection of SARS-CoV-2 antibodies are a tool suitable for population-based seroepidemiology studies in children and highly acceptable in children and adults, as venipuncture is no longer necessary.
Katja Hoschler, Samreen Ijaz, Nick Andrews, Sammy Ho, Steve Dicks, Keerthana Jegatheesan, John Poh, Lenesha Warrener, Thivya Kankeyan, Frances Baawuah, Joanne Beckmann, Ifeanichukwu O Okike, Shazaad Ahmad, Joanna Garstang, Andrew J Brent, Bernadette Brent, Felicity Aiano, Kevin E Brown, Mary E Ramsay, David Brown, John V Parry, Shamez N Ladhani, Maria Zambon

1980 related Products with: SARS Antibody Testing in Children: Development of Oral Fluid Assays for IgG Measurements.

100 ug100 TESTS0.1ml (1mg/ml)0.2 mg0.25 mg1 ml100ug Lyophilized48 assays 1 Set16-22 Sample Kit1 Set1 Set

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