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#34562885   2021/08/25 To Up

Recent Advances in Novel Lateral Flow Technologies for Detection of COVID-19.

The development of reliable and robust diagnostic tests is one of the most efficient methods to limit the spread of coronavirus disease 2019 (COVID-19), which is caused by the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). However, most laboratory diagnostics for COVID-19, such as enzyme-linked immunosorbent assay (ELISA) and reverse transcriptase-polymerase chain reaction (RT-PCR), are expensive, time-consuming, and require highly trained professional operators. On the other hand, the lateral flow immunoassay (LFIA) is a simpler, cheaper device that can be operated by unskilled personnel easily. Unfortunately, the current technique has some limitations, mainly inaccuracy in detection. This review article aims to highlight recent advances in novel lateral flow technologies for detecting SARS-CoV-2 as well as innovative approaches to achieve highly sensitive and specific point-of-care testing. Lastly, we discuss future perspectives on how smartphones and Artificial Intelligence (AI) can be integrated to revolutionize disease detection as well as disease control and surveillance.
Wesley Wei-Wen Hsiao, Trong-Nghia Le, Dinh Minh Pham, Hui-Hsin Ko, Huan-Cheng Chang, Cheng-Chung Lee, Neha Sharma, Cheng-Kang Lee, Wei-Hung Chiang

1589 related Products with: Recent Advances in Novel Lateral Flow Technologies for Detection of COVID-19.

1 G 5 G100ug Lyophilized100ug Lyophilized500 MG

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#34562841   2021/09/22 To Up

Minocycline attenuates oxidative and inflammatory injury in a intestinal perforation induced septic lung injury model via down-regulating lncRNA MALAT1 expression.

Oxidative stress and inflammatory responses play an important role in acute lung injury (ALI). Although minocycline (MINO) has anti-inflammatory effects and is a promising candidate in treating inflammatory diseases, the effect of MINO on ALI during sepsis is still unclear.
Ning Cui, Yong Liang, Junyu Wang, Bo Liu, Bing Wei, Yu Zhao

1313 related Products with: Minocycline attenuates oxidative and inflammatory injury in a intestinal perforation induced septic lung injury model via down-regulating lncRNA MALAT1 expression.

2ug100ug Lyophilized100ul100ug

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#34562639   2021/09/22 To Up

Trypsin-mediated sensitization to ferroptosis increases the severity of pancreatitis in mice.

Pancreatitis is characterized by acinar cell death and persistent inflammation. Ferroptosis is a type of lipid peroxidation-dependent necrosis, which is negatively regulated by GPX4. We studied how trypsin, a serine protease secreted by pancreatic acinar cells, affects the contribution of ferroptosis to triggering pancreatitis.
Ke Liu, Jiao Liu, Borong Zou, Changfeng Li, Herbert J Zeh, Rui Kang, Guido Kroemer, Jun Huang, Daolin Tang

1077 related Products with: Trypsin-mediated sensitization to ferroptosis increases the severity of pancreatitis in mice.

500 gm.96 wells (1 kit)1 mg20 mg100 100ml25

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#34562473   2021/09/22 To Up

Intranasal administration of DHED protects against exhaustive exercise-induced brain injury in rats.

DHED (10β,17β-dihydroxyestra-1,4-dien-3-one) is a brain-selective prodrug of 17β-estradiol and has been reported to have a strong neuroprotective effect. In this study, the exhaustive swimming rat model was used to investigate the therapeutic effects and mechanisms of intranasal DHED treatment. Male eight-week-old healthy Sprague Dawley rats were randomly divided into three groups: control group (Cont), exhaustive swimming (ES), and DHED + exhaustive swimming (DHED). The open-field test and beam-walking test were performed to measure exploratory behavior and general activity in rats. Immunofluorescence staining, western blotting, ELISA analysis and related assay kits were applied to measure brain damage, inflammatory cytokines, and apoptosis pathways. Behavioral data shows that DHED intranasal administration can prevent neurobehavioral impairment caused by exhaustive swimming. Using a series of bioanalytical assays, we demonstrated that DHED markedly abated neuronal injury compared to the exhaustive swimming group, as evidenced by the reduced expression of apoptosis-regulated proteins, the improvement of neural survival, and the prevention of myelin loss. In addition, mitochondrial fission was attenuated distinctly, and a dynamic equilibrium was restored. Intranasal administration of DHED likewise significantly suppressed reactive gliosis and the release of inflammatory cytokines in the rat cerebral motor cortex. Consistent with previous reports, DHED treatment ameliorated changes of excitatory neurotransmitters. These results provide strong support for the promising therapeutic effects of DHED on neuroprotection during exhaustive swimming. The underlying mechanisms may rely on mitochondrial dynamics, neuroinflammation, and the balance of neurotransmitters.
Guangcong Peng, Huaping Zheng, Chunyi Wu, Chongyun Wu, Xu Ma, Jing Xiong, Jun Hou, Limei Zhang, Luodan Yang, Hongying Pan

1425 related Products with: Intranasal administration of DHED protects against exhaustive exercise-induced brain injury in rats.

50 ul96 tests96T5ug100ug100 μg50ug2ug96 tests

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#34562431   2021/09/22 To Up

Seroprevalence and molecular characterization of Toxoplasma gondii infecting ruminants in the North-West of Egypt.

Toxoplasma gondii is a coccidian parasite known for its heavy toll on people and livestock. It can cause abortion and a variety of congenital diseases. The current study aimed to examine some seroprevalence and molecular attributes of T. gondii obtained from ruminants in the North-West of Egypt. Specimens were random selected from five different locations in Alexandria and Matrouh governorates. A total of 483 blood samples, collected from 96 mixed flocks, were screened for anti-T. gondii IgG antibodies using enzyme-linked immunosorbent assay (ELISA). The seropositive results were then confirmed using polymerase chain reaction (PCR) primers for the B1 and P30 genes. Specific PCR products were selected for sequencing and alignment against the GenBank, where phylogeny has been examined using the maximum likelihood, neighbor-joining, and maximum parsimony in MEGA6. ELISA confirmed the presence of T. gondii in 188 of the investigated samples (38.92%), indicating a higher prevalence in camels (64.51%) and sheep (43.75%) as compared to goats (27.93 %) and cattle (13.46%). PCR confirmed the presence of T. gondii-specific sequences in 159 seropositive specimens, with homology between 98.3 and 100%. The genetic distances between the investigated variants ranged from 0.1 to 0.9, and 7 single nucleotide polymorphisms (SNPs), were identified in the examined T. gondii specimens. The camel T. gondii parasite, isolated from Matrouh, showed a 100% homology with the most dangerous reference strains of T. gondii-RH in the GenBank. Our results showed that B1 and P30-specific PCR could detect T. gondii in blood samples more accurately than ELISA. In addition, the statistical analysis of our data indicated that species, age, sex, and animal location were all risk factors for toxoplasmosis. These findings are likely to boost disease control and help contain the spread of T. gondii infections.
Reham Abdel-Halim Khattab, Safaa Mohamed Barghash, Osama Mohammad Sayed Mostafa, Sahar Ali Allam, Hoda Abdel-Halim Taha, Ameen Abd El-Baqi Ashour

2914 related Products with: Seroprevalence and molecular characterization of Toxoplasma gondii infecting ruminants in the North-West of Egypt.

1 mg100 5 G100 µg100 1100

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#34562263   2021/09/25 To Up

Immunity after COVID-19 and vaccination: follow-up study over 1 year among medical personnel.

The long-term course of immunity among individuals with a history of COVID-19, in particular among those who received a booster vaccination, has not been well defined so far.
Vivian Glück, Sonja Grobecker, Josef Köstler, Leonid Tydykov, Manuela Bertok, Tanja Weidlich, Christine Gottwald, Bernd Salzberger, Ralf Wagner, Florian Zeman, Michael Koller, André Gessner, Barbara Schmidt, Thomas Glück, David Peterhoff

2656 related Products with: Immunity after COVID-19 and vaccination: follow-up study over 1 year among medical personnel.

25 mg1 mg1 mg100ug0.1 mg 5 G 1 G 1 G50 ug

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#34561996   2021/09/22 To Up

Oxaliplatin facilitates tumor-infiltration of T cells and natural-killer cells for enhanced tumor immunotherapy in lung cancer model.

Platinum is reported to have adjuvant immune properties, whether oxaliplatin (OXA) could be utilized to synergize with anti-programmed cell death-1 (PD-1) antibody or anti-NKG2D (natural-killer group 2, member D) antibody is investigated.
Min Xin, Dan Lin, Nahan Yan, Huiling Li, Jieping Li, Zongming Huang

1911 related Products with: Oxaliplatin facilitates tumor-infiltration of T cells and natural-killer cells for enhanced tumor immunotherapy in lung cancer model.



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#34561731   2021/09/24 To Up

Are infectious reproductive pathogens of large ruminants a threat to improving food security? An investigation from Cambodia.

In Cambodia, information on common pathogens causing reproductive losses in cattle and buffalo are lacking, despite there being a need to address livestock health to enhance food security. We analysed stored buffalo (n = 29) and cattle (n = 471) serum samples collected in 2016 using commercially available enzyme-linked immunosorbent assay kits. Antibodies to Neospora caninum, bovine viral diarrhoea virus (BVDV), Leptospira interrogans serovar Hardjo and Brucella abortus were detected in buffalo samples at 79.3% (95% CI 64.6-94.0), 3.4% (95% CI 0-10.0), 0% and 0%, respectively, and in cattle at 4.2% (95% CI 2.4-6.0), 6.4% (95% CI 4.2-8.6), 8.1% (95% CI 5.6-10.6) and 0%, respectively. The high N. caninum seroprevalence in buffalo was associated with increasing age, with buffalo having a 13.1% chance of being seropositive at birth, increasing to 99.4% by age 7 (p = 0.045). This suggests a predominance of horizontal transmission, possibly from exposure to faeces from free-roaming village dogs. Cattle L. interrogans serovar Hardjo seroprevalence was highest in Tbong Khmum province (20.4%) compared to other provinces (p < 0.001), and may be compromising bovine fertility and creating a zoonotic risk for smallholders who may contract leptospirosis from farm work. These high infection rates prompt further research to determine: to what extent these pathogens are linked to the low reproductive efficiency observed in large ruminants in Cambodia, the risk factors to pathogen exposure and appropriate strategies to reduce these risks. Low BVDV and B. abortus exposure is an important observation. Increasing large ruminant livestock trade into the country will require improved biosecurity and disease surveillance to prevent their emergence. An enhanced understanding of the status of infectious reproductive livestock pathogens in Cambodia can assist development projects to make evidence-based strategies to enhance cattle and buffalo health and improve food security.
Luisa Olmo, Michael Philipp Reichel, Peter Andrew Windsor, Sothoeun Suon, Lloyd Christian Wahl, Peter Campbell Thomson, Russell David Bush

1765 related Products with: Are infectious reproductive pathogens of large ruminants a threat to improving food security? An investigation from Cambodia.

100 1 mg1 module1 kit(96 Wells)1 module1 mL100 μg1 ml1 kit(96 Wells)500 tests1 module100

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#34561488   2021/09/24 To Up

Saturation transfer MRI is sensitive to neurochemical changes in the rat brain due to chronic unpredictable mild stress.

Chemical exchange saturation transfer (CEST) MRI was performed for the evaluation of cerebral metabolic changes in a rat model of depressive-like disease induced by chronic unpredictable mild stress (CUMS). CEST Z-spectra were acquired on a 7 T MRI with two saturation B amplitudes (0.5 and 0.75 µT) to measure the magnetization transfer ratio (MTR), CEST and relayed nuclear Overhauser effect (rNOE). Cerebral cortex and hippocampus were examined in two groups of animals: healthy control (n = 10) and stressed (n = 14), the latter of which was exposed to eight weeks of the CUMS protocol. The stressed group Z-spectrum parameters, primarily MTRs, were significantly lower than in controls, at all selected frequency offsets (3.5, 3.0, 2.0, - 3.2, - 3.6 ppm) in the cortex (the largest difference of ~ 3.5% at - 3.6 ppm, p = 0.0005) and the hippocampus (MTRs measured with a B = 0.5 µT). The hippocampal rNOE contributions decreased significantly in the stressed brains. Glutamate concentration (assessed using ELISA) and MTR at 3 ppm correlated positively in both brain regions. GABA concentration also correlated positively with CEST contributions in both cerebral areas, while such correlation with MTR was positive in hippocampus, and nonsignificant in cortex. Results indicate that CEST is sensitive to neurometabolic changes following chronic stress exposure.
Anna Pankowska, Agata Chudzik, Tymoteusz Słowik, Artur Łazorczyk, Katarzyna Kochalska, Marta Andres-Mach, Wilfred W Lam, Radosław Pietura, Radosław Rola, Greg J Stanisz, Anna Orzyłowska

2102 related Products with: Saturation transfer MRI is sensitive to neurochemical changes in the rat brain due to chronic unpredictable mild stress.

1 G1mg200 ug96 wells500 gm.1 kit100 μg

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#34561399   2021/09/23 To Up

Could the Pittsburgh Severity Score guide the treatment of esophageal perforation? Experience of a single referral center.

Esophageal perforation (EP) is characterized by high morbidity and mortality. The Pittsburgh Severity Score (PSS) is a scoring system based on clinical factors at the time of EP presentation, intended to guide treatment. The aim of the study is to verify PSS usefulness in stratifying EP severity and in guiding clinical decisions.
Moletta Lucia, Pierobon Elisa Sefora, Capovilla Giovanni, Valotto Giovanni, Gavagna Laura, Provenzano Luca, Zanchettin Gianpietro, Salvador Renato, Costantini Mario, Merigliano Stefano, Valmasoni Michele

2317 related Products with: Could the Pittsburgh Severity Score guide the treatment of esophageal perforation? Experience of a single referral center.

100.00 ul1200 units100.00 ul200 units

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