Search results for: ER
#33098715 2020/10/24 To Up
A temporal hierarchy underpins the transcription factor-DNA interactome of the maize UPR.Adverse environmental conditions reduce crop productivity and often increase a load of unfolded or misfolded proteins in the endoplasmic reticulum (ER). This potentially lethal condition, known as ER stress, is buffered by the unfolded protein response (UPR), a set of signaling pathways designed to either recover ER functionality or ignite programmed cell death. Despite the biological significance of the UPR to the life of the organism, the regulatory transcriptional landscape underpinning ER stress management is largely unmapped, especially in crops. To fill this significant knowledge gap, we performed a large-scale systems-level analysis of protein-DNA interaction (PDI) network in maize (Zea mays). Using 23 promoter fragments of six UPR marker genes in a high-throughput enhanced yeast one-hybrid (eY1H) assay, we identified a highly interconnected network of 262 transcription factors (TFs) associated with significant biological traits and 831 PDIs underlying the UPR. We established a temporal hierarchy of TF binding to gene promoters within the same family as well as across different families of TFs. Cistrome analysis revealed the dynamic activities of a variety of cis-regulatory elements (CREs) in ER stress-responsive gene promoters. By integrating the cistrome results into a TF network analysis, we mapped a subnetwork of TFs associated with a CRE that may contribute to the UPR management. Finally, we validated the role of a predicted network hub gene using the Arabidopsis system. The PDIs, TF networks and CREs identified in our work are foundational resources for understanding transcription regulatory mechanisms in the stress responses and crop improvement.
Dae Kwan Ko, Federica Brandizzi
2887 related Products with: A temporal hierarchy underpins the transcription factor-DNA interactome of the maize UPR.100ul200ug0.1 mgKit 25UG200ug1 ml100.00 ul
#33098669 2020/10/24 To Up
Plasma membrane Ca ATPase 1 (PMCA1) but not PMCA4 is critical for B cell development and Ca homeostasis in mice.The amplitude and duration of Ca signaling is crucial for B cell development and self-tolerance; however, the mechanisms for terminating Ca signals in B cells have not been determined. In lymphocytes, plasma membrane Ca ATPase (PMCA) isoforms 1 and 4 (PMCA1 and PMCA4, aka ATP2B1 and ATP2B4) are the main candidates for expelling Ca from the cell through the plasma membrane. We report here that Pmca4 (Atp2b4) knockout mice had normal B cell development, while mice with a conditional knockout of Pmca1 (Atp2b1) had greatly reduced numbers of B cells, particularly splenic follicular B cells (FOBs), marginal zone B cells (MZBs), and peritoneal B-1a cells. Mouse and naïve human B cells showed only PMCA1 expression and no PMCA4 by western blot, in contrast to T cells, which did express PMCA4. Calcium handling was normal in Pmca4 B cells, but Pmca1 knockout B cells had elevated basal levels of Ca , elevated levels in ER stores, and reduced Ca clearance. These findings show that the PMCA1 isoform alone is required to ensure normal B cell Ca signaling and development, which may have implications for therapeutic targeting of PMCAs and Ca in B cells. This article is protected by copyright. All rights reserved.
Mark Korthals, Laura Tech, Kristina Langnaese, Anna Gottfried, Johannes Hradsky, Ulrich Thomas, Ana Claudia Zenclussen, Monika Brunner-Weinzierl, Kerry Tedford, Klaus-Dieter Fischer
2093 related Products with: Plasma membrane Ca ATPase 1 (PMCA1) but not PMCA4 is critical for B cell development and Ca homeostasis in mice.10 mg10 mg25 mg100ug Lyophilized100ug Lyophilized25 mg100ug Lyophilized25 mg100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized
#33098638 2020/10/24 To Up
Cross-talk between the ER pathway and the lncRNA MAFG-AS1/miR-339-5p/ CDK2 axis promotes progression of ER+ breast cancer and confers tamoxifen resistance.Hormone receptor-positive breast cancer accounts for around 75% of breast cancers. The estrogen receptor pathway promotes tumor progression and endocrine resistance. Recently, the cross-talk between the ER signaling pathway and cell cycle regulation has been identified. It is necessary to determine the underlying molecular mechanisms involved in the ER signaling pathway and find new target genes for prognosis and drug resistance in ER+ breast cancer. In this study, lncRNA MAFG-AS1 was shown to be up-regulated and associated with poor prognosis in ER+ breast cancer. Functionally, down-regulation of MAFG-AS1 could inhibit cell proliferation and promote apoptosis. In addition, MAFG-AS1 which contained an estrogen-responsive element could promote CDK2 expression by sponging miR-339-5p. Subsequently, MAFG-AS1 and CDK2 were found to be up-regulated in tamoxifen-resistant MCF-7 cells. Cross-talk between the ER signaling pathway and cell cycle conducted by MAFG-AS1 and CDK2 could promote tamoxifen resistance. In conclusion, our study indicated that estrogen-responsive lncRNA MAFG-AS1 up-regulated CDK2 by sponging miR-339-5p, which promoted ER+ breast cancer proliferation. Cross-talk between the ER signaling pathway and cell cycle suggested that lncRNA MAFG-AS1 is a potential biomarker and therapeutic target in ER+ breast cancer. CDK2 inhibitors may be applied to endocrine resistance therapy.
Jing Feng, Ti Wen, Zhi Li, Liang Feng, Lu Zhou, Zichang Yang, Lu Xu, Sha Shi, Kezuo Hou, Jiming Shen, Xu Han, Yuee Teng
2162 related Products with: Cross-talk between the ER pathway and the lncRNA MAFG-AS1/miR-339-5p/ CDK2 axis promotes progression of ER+ breast cancer and confers tamoxifen resistance.100ul100ul100ul100ul100ul100ul500 MG
#33098567 2020/10/24 To Up
Reduced magnitude and shifted seasonality of CO sink by experimental warming in a coastal wetland.Coastal wetlands have the highest carbon sequestration rate per unit area among all unmanaged natural ecosystems. However, how magnitude and seasonality of CO sink in coastal wetlands will respond to future climate warming remain unclear. Here, based on measurements of ecosystem CO fluxes in a field experiment in the Yellow River Delta, we found that experimental warming (i.e., a 2.4 °C increase in soil temperature) reduced net ecosystem productivity (NEP) by 23.7% across two growing seasons of 2017-2018. Such a reduction in NEP resulted from the greater decrease in gross primary productivity (GPP) than ecosystem respiration (ER) under warming. The negative warming effect on NEP mainly occurred in summer (-43.9%) but not in autumn (+61.3%), leading to a shifted NEP seasonality under warming. Further analyses showed that the warming effects on ecosystem CO exchange were mainly controlled by soil salinity and its corresponding impacts on species composition. For example, warming increased soil salinity (+35.0%), reduced total aboveground biomass (-9.9%), and benefited the growth of plant species with high salt tolerance and late peak growth. To the best of our knowledge, this study provides the first experimental evidence on the reduced magnitude and shifted seasonality of CO exchange under climate warming in coastal wetlands. These findings underscore the high vulnerability of wetland CO sink in coastal regions under future climate change.
Baoyu Sun, Liming Yan, Ming Jiang, Xinge Li, Guangxuan Han, Jianyang Xia
2905 related Products with: Reduced magnitude and shifted seasonality of CO sink by experimental warming in a coastal wetland.100ug Lyophilized100ug Lyophilized100ug Lyophilized
#33098103 2020/10/23 To Up
Novel PGM3 compound heterozygous variants with IgE-related dermatitis, lymphopenia, without syndromic features.Phosphoglucomutase-3 (PGM3) deficiency is a congenital disorder of glycosylation (CDG) with hyperimmunoglobulin IgE, atopy and a variable immunological phenotype; most reported patients display dysmorphic features. The aim of the study was to characterize the genotype and phenotype of individuals with newly identified compound heterozygous variants in the phosphate-binding domain of PGM3 in order to better understand phenotypic differences between these patients and published cases.
Ana García-García, Monserrat Buendia Arellano, Àngela Deyà-Martínez, Jaime Lozano Blasco, Mercedes Serrano, Ana Van Den Rym, Blanca García-Solis, Ana Esteve-Solé, Luo Yiyi, Alexandru Vlagea, Xavier Solanich, Megan R Fisher, Jonathan J Lyons, Rebeca Pérez de Diego, Laia Alsina
2587 related Products with: Novel PGM3 compound heterozygous variants with IgE-related dermatitis, lymphopenia, without syndromic features.10ml0.2 mg 6 ml Ready-to-use 25 100ul12ml5 mg100ug100ug1kit100
#33097730 2020/10/23 To Up
Dopants fixation of Ruthenium for boosting acidic oxygen evolution stability and activity.Designing highly durable and active electrocatalysts applied in polymer electrolyte membrane (PEM) electrolyzer for the oxygen evolution reaction remains a grand challenge due to the high dissolution of catalysts in acidic electrolyte. Hindering formation of oxygen vacancies by tuning the electronic structure of catalysts to improve the durability and activity in acidic electrolyte was theoretically effective but rarely reported. Herein we demonstrated rationally tuning electronic structure of RuO with introducing W and Er, which significantly increased oxygen vacancy formation energy. The representative WErRuO required a super-low overpotential of 168 mV (10 mA cm) accompanied with a record stability of 500 h in acidic electrolyte. More remarkably, it could operate steadily for 120 h (100 mA cm) in PEM device. Density functional theory calculations revealed co-doping of W and Er tuned electronic structure of RuO by charge redistribution, which significantly prohibited formation of soluble Ru and lowered adsorption energies for oxygen intermediates.
Shaoyun Hao, Min Liu, Junjie Pan, Xiangnan Liu, Xiaoli Tan, Nan Xu, Yi He, Lecheng Lei, Xingwang Zhang
1399 related Products with: Dopants fixation of Ruthenium for boosting acidic oxygen evolution stability and activity.50 mL 96 Tests 100 assays 1L0.1ml (1mg/ml)100 mg50 ug 50 ug 5 G1 kit
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#33097496 2020/10/23 To Up
Epidemiology of basal-like and luminal breast cancers among black women in the AMBER consortium.Evidence suggests etiologic heterogeneity among breast cancer subtypes. Previous studies with six-marker immunohistochemical classification of intrinsic subtypes included small numbers of black women.
Halei C Benefield, Gary R Zirpoli, Emma H Allott, Yue Shan, Amber N Hurson, Angela R Omilian, Thaer Khoury, Chi-Chen Hong, Andrew F Olshan, Traci N Bethea, Elisa V Bandera, Julie R Palmer, Christine B Ambrosone, Melissa A Troester
1834 related Products with: Epidemiology of basal-like and luminal breast cancers among black women in the AMBER consortium.
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#33097191 2020/10/06 To Up
Could lymph node evaluation be eliminated in nearly 50% of women with early stage ER/PR positive breast cancer?The Society of Surgical Oncology introduced guidance discouraging routine axillary staging in women 70 years or older with invasive, clinically node negative, hormone-receptor positive breast cancer. Due to concerns this could result in patients missing necessary treatment, researchers from the Mayo Clinic developed a rule to distinguish between those at low/high-risk of having positive nodes. The purpose of this study was to validate the Mayo Clinic rule in women of all ages.
Juan Ruiz, Gerson Maldonado, Elizabeth Ablah, Hayrettin Okut, Jared Reyes, Karson Quinn, Patty L Tenofsky
1099 related Products with: Could lymph node evaluation be eliminated in nearly 50% of women with early stage ER/PR positive breast cancer?
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