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Search results for: Elisa kits rec

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#31278094   2019/07/04 To Up

The effect of resveratrol supplementation on the expression levels of factors associated with cellular senescence and sCD163/sTWEAK ratio in patients with type 2 diabetes mellitus: study protocol for a double-blind controlled randomised clinical trial.

Over the past decades, the number of people with type 2 diabetes (T2D) has increased globally. One of the major complications in these patients is cardiovascular disease; it seems that the cell proliferation inhibition can improve vascular function in these patients. It is proposed that peroxisome proliferator-activated receptor alpha (PPARα) can induce cell cycle arrest via cyclin-dependent kinase inhibitor 2A (p16) activation. Also, it has been shown that phosphorylated tumour suppressor protein p53 is involved in cell senescence by cyclin-dependent kinase inhibitor 1 (p21) upregulation. Resveratrol is a natural polyphenol and appears to improve the vascular function through the mentioned pathways. We will aim to evaluate the effects of resveratrol supplementation on mRNA expression of PPARα, p53, p21 and p16 in patients with T2D. We will also measure serum levels of cluster of differentiation 163 (CD163) and tumour necrosis factor (TNF)-like weak inducer of apoptosis (TWEAK) as the indicators of cardiovascular status.
Shima Abdollahi, Amin Salehi-Abargouei, Mahtab Tabatabaie, Mohammad Hasan Sheikhha, Hossein Fallahzadeh, Masoud Rahmanian, Omid Toupchian, Elham Karimi-Nazari, Hassan Mozaffari-Khosravi

2330 related Products with: The effect of resveratrol supplementation on the expression levels of factors associated with cellular senescence and sCD163/sTWEAK ratio in patients with type 2 diabetes mellitus: study protocol for a double-blind controlled randomised clinical trial.

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#29109181   2017/11/06 To Up

Achievements of an eradication programme against caprine arthritis encephalitis virus in South Tyrol, Italy.

Small ruminant lentivirus infections in goats affect both production and animal welfare. This represents a threat to the qualitative and quantitative growth of goat farming, recently observed in mountainous regions such as the Autonomous Province of Bolzano - South Tyrol (Italy). To monitor and eradicate the caprine arthritis encephalitis virus in this goat population, a compulsory eradication campaign was launched, based on a strict census of small ruminants and yearly serological testing of all animals, followed by the consequent culling of seropositive individuals. The campaign succeeded in completely eliminating cases of clinical disease in goats, while drastically reducing the seroprevalence at the herd as well as individual animal level. The serological outcome of the introduced control measures was determined using commercially available ELISA kits, demonstrating their suitability for use in this type of campaign, aimed at reducing seroprevalence as well as clinical manifestations of these infections. However, this clear success is diminished by the failure to achieve a complete eradication of these viruses. The reasons leading to the observed tailing phenomenon and the occurrence of new infections in already sanitised flocks are discussed and implementation of further measures are proposed.
Alexander Tavella, Astrid Bettini, Marco Ceol, Paolo Zambotto, Ernst Stifter, Natashia Kusstatscher, Rosalba Lombardi, Stefano Nardeli, Maria Serena Beato, Katia Capello, Giuseppe Bertoni

1615 related Products with: Achievements of an eradication programme against caprine arthritis encephalitis virus in South Tyrol, Italy.

1 mg1 mL100 1001 mg1 mL1 mL100 µg1 mg1 mg1 mL100

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#28713178   2013/11/28 To Up

Glucose transporter-4 in white blood cells of young and old sled dogs: a model for human biomarker development.

The insulin responsive glucose transporter, GLUT4 is found predominantly in muscle and adipose cells. Maratou and others (2007) reported that there is GLUT4 in white blood cells (WBC) collected from human subjects in response to insulin activation. This study was designed to validate the presence of GLUT4 in white blood cells of sled dogs and furthermore to investigate whether changes in levels of the GLUT4 protein might be associated with aging. Additionally, we examined the blood insulin concentration of two populations of dogs, young and old, before and after a meal to observe their insulin response. It is documented in skeletal muscle that GLUT4 expression is increased as a result of conditioning, making sled dogs an excellent model in the circumpolar north for studying the effects of exercise, nutrition and diabetes (Felsburg 2002; Kararli 2006). Blood was withdrawn from 11 healthy sled dogs: 6 young (1-5 years) and physically fit, conditioned for racing and 5 old (7-13 years), retired from racing. The insulin response was determined using blood plasma and ELISA. The buffy coat (containing WBC) was collected with a glass pipette after centrifugation and washed and suspended in 1x phosphate buffer. GLUT4 was measured using ELISA kits (USCN Life Sciences). The results validate that GLUT4 is present in white blood cells in sled dogs. Age had no significant effect in the concentration of GLUT4 between the populations of old and young dogs. A significant difference in insulin levels pre and post meal in young (0.13 ± 0.03 ng/mL (pre), 0.22 ± 0.04 ng/mL (post), p < 0.05) and old (0.13 ± 0.02 ng/mL (pre), 0.22 ± 0.03 ng/mL (post), p < 0.05) dogs was observed, displaying the typical postprandial insulin spike. No significant difference was found in insulin concentration comparing old versus young dogs. Our data shows that white blood cells in young (40.4 ± 2.4 ng/mL) and old (35.3 ± 8.8 ng/mL) sled dogs have quantifiable but non-significant different GLUT4 levels (p > 0.05). Detecting GLUT4 via an ELISA in white blood cells, opens up minimally invasive avenues for studying the underlying molecular mechanisms associated with insulin resistance in more complex, dynamic and physiological systems. This project was the first step in developing a protocol for this simple, technique with a potential clinical application for diagnosing insulin resistance.
Theresia M Schnurr, Arleigh J Reynolds, Lawrence K Duffy, Kriya L Dunlap

1436 related Products with: Glucose transporter-4 in white blood cells of young and old sled dogs: a model for human biomarker development.

96 assays50 mg1.00 flask1.00 flask100 extractions 100 UG100 μg100Tests25 100ug Lyophilized

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#24408313   2014/01/08 To Up

Prevalence of paratuberculosis in cattle and control measures within the herd influence the performance of ELISA tests.

Commercial ELISA kits are widely used in the diagnosis of paratuberculosis of dairy cattle. It is critically important to understand the influences on these test results and their relation to faecal culture (FC) results in order to interpret the findings and to make decisions concerning serial testing and control measures. A total of 1021 cattle (423 FC positive, 598 FC negative) from 14 Mycobacterium avium subspecies paratuberculosis (MAP) positive herds were tested with four ELISA systems and FC simultaneously to calculate the kappa coefficients for the agreement of the different ELISA systems as well as find influencing factors. For the agreement of FC and ELISA, the kappa coefficients were low and ranged from 0.19 to 0.24, whereas, results of the different ELISA were consistently high (0.74-0.90). Agreement with FC was enhanced with the duration of control (P≤0.001) and the lactation number (P≤0.01), and reduced with within-herd prevalence (P≤0.001). There were substantial differences in the detection rate of low (15-24 per cent) and high (85-100 per cent) MAP shedders. In conclusion, the factors shown to influence test sensitivity, should be taken into account for validation and interpretation of ELISA tests. The benefit of serial ELISA testing is low.
K Donat, K Schlotter, G Erhardt, H R Brandt

2357 related Products with: Prevalence of paratuberculosis in cattle and control measures within the herd influence the performance of ELISA tests.

96 tests196 tests96 tests96 tests96 tests

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#22027187   2011/10/25 To Up

Transfer of tumour necrosis factor-α via colostrum to foals.

This study aimed to determine whether TNF-α is transferred to equine neonates via colostrum and the relationship between TNF-α and IgG concentrations in the equine neonate. Colostrum, presuckle and postsuckle foal serum samples were collected from healthy mares and their foals. Equine TNF-α ELISA and IgG SRID kits were used to determine the concentrations of TNF-α and IgG, respectively. Statistical analysis was performed using the Spearman rank correlation. TNF-α concentrations in all presuckle foal serum were below the limit of detection in 15/16 foals and increased in postsuckle foal serum to a mean concentration of 7.7 x 10(4) pg/ml. TNF-α concentrations in postsuckle foal serum and colostrum showed significant correlation (rho=0.668; P=0.005). However, TNF-α and IgG concentrations in colostrum or postsuckle foal serum did not correlate (rho<-0.016; P>0.05). Ratios of TNF-α/IgG in colostrum or postsuckle foal serum showed significant correlation (rho=0.750; P=0.0008). These results indicate that TNF-α is transferred to the foal via colostrum absorption and may play a role in early immunity.
E J Secor, M B Matychak, M J B Felippe

2701 related Products with: Transfer of tumour necrosis factor-α via colostrum to foals.

20 5ug96T100 ug/vial0.1 mg100ug/vial96T1 mg96T 96T/Kit 5ug5ug

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#21257399   // To Up

Technical and financial evaluation of assays for progesterone in canine practice in the UK.

The concentration of progesterone was measured in 60 plasma samples from bitches at various stages of the oestrous cycle, using commercially available quantitative and semi-quantitative ELISA test kits, as well as by two commercial laboratories undertaking radioimmunoassay (RIA). The RIA, which was assumed to be the 'gold standard' in terms of reliability and accuracy, was the most expensive method when analysing more than one sample per week, and had the longest delay in obtaining results, but had minimal requirements for practice staff time. When compared with the RIA, the quantitative ELISA had a strong positive correlation (r=0.97, P<0.05) and a sensitivity and specificity of 70.6 per cent and 100.0 per cent, respectively, and positive and negative predictive values of 100.0 per cent and 71.0 per cent, respectively, with an overall accuracy of 90.0 per cent. This method was the least expensive when analysing five or more samples per week, but had longer turnaround times than that of the semi-quantitative ELISA and required more staff time. When compared with the RIA, the semi-quantitative ELISA had a sensitivity and specificity of 100.0 per cent and 95.5 per cent, respectively, and positive and negative predictive values of 73.9 per cent and 77.8 per cent, respectively, with an overall accuracy of 89.2 per cent. This method was more expensive than the quantitative ELISA when analysing five or more samples per week, but had the shortest turnaround time and low requirements in terms of staff time.
R Moxon, D Copley, G C W England

1777 related Products with: Technical and financial evaluation of assays for progesterone in canine practice in the UK.

100 assays500 MG25ml100 assays96 assays 100 assays

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#18424848   // To Up

Use of cardiac troponin kits for the qualitative determination of myocardial cell damage due to traumatic reticuloperitonitis in cattle.

This study was designed to investigate whether kits to measure circulating cardiac troponin-I (cTn-I) and cardiac troponin-T (cTn-T) can be used to determine myocardial cell damage in cattle with traumatic reticuloperitonitis (trp). Twenty cattle with trp were compared with 10 clinically healthy cattle. cTn-I and cTn-T were determined qualitatively and cTn-I was determined quantitatively; biochemical analyses were also performed on both groups. The mean serum concentrations of total protein, globulin, glucose and calcium, and the mean activities of creatine kinase mb, aspartate aminotransferase, lactate dehydrogenase and gamma-glutamyl transferase were higher in the cattle with trp than in the control group. The cTn-I and cTn-T kits both gave positive results in three of the cattle with trp and the quantitative measurement of cTn-I was positive in 11 of the trp cases. Both tests were negative in the healthy cattle.
V Gunes, G Atalan, M Citil, H M Erdogan

1840 related Products with: Use of cardiac troponin kits for the qualitative determination of myocardial cell damage due to traumatic reticuloperitonitis in cattle.

96 assays1 kit1 kit500 gm.500 ml

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#16006640   // To Up

Comparison of two commercial ELISAs for the serological diagnosis of salmonellosis in pigs.

Serum samples from 361 pigs (194 fattening pigs and 167 sows) were examined by means of two commercial ELISAs (Svanovir; Svanova Biotech and Salmotype; Labor Diagnostik) used for the serological diagnosis of salmonellosis in pigs; 211 of the samples came from farms of known bacteriological status and the other 150 were collected randomly from 60 farms of unknown status. The ELISAs were done according to the manufacturers' directions and the samples were categorised accordingly. The results were compared by using a linear regression analysis and by the calculation of Kappa values. To try to improve the agreement between the tests, the raw optical densities (ODS) were transformed to sample/positive (S/P) ratios by using the positive control as a reference, and cut-off values for these S/P ratios were calculated by means of a receiver operating characteristic (ROC) analysis. All but two of the known infected farms were recognised as such by both tests. However, the correlation of the raw ODS for individual pigs was poor (r=0.546) and had a Kappa value for the results categorised according to the manufacturers' recommendations of 0.191. On some farms the correlation was high (r=0.97) but on others it was low (r=0.05) with no apparent reason for the difference. The S/P ratios did not improve the agreement (Kappa=0.25).
W Mejía, J Casal, E Mateu, M Martín

1420 related Products with: Comparison of two commercial ELISAs for the serological diagnosis of salmonellosis in pigs.

100 G1250 mg 1 G 1 G 5 G

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#14653341   // To Up

Specificity of three ELISA-gE kits for screening pig meat for antibodies to Aujeszky's disease.

Muscle samples (20 g) from 2025 pig carcases from Aujeszky's disease-free holdings were collected at the slaughterhouse. The samples were frozen and thawed to obtain meat juice, which was then analysed by three ELISA-gE test kits in parallel, to assess their specificity. After two cycles of freezing and thawing, 2.2 per cent of the samples were dry. Three times more of the samples from the sow carcases than from the finisher carcases yielded insufficient juice (< 220 microl). To validate the results of the specificity study, the sensitivity of the test kits was evaluated on 45 samples from gE-seropositive sows. On the basis of the results from 1879 samples, the specificity of the ELISA-gE kits was between 0.995 and 1.000, depending on the classification of the doubtful results. In the case of a positive or doubtful result, it proved useful to repeat the test on the same sample, in order to limit the number of false positive results.
K De Lange, N Haddad, M F Le Potier, C Agier, M Le Vée, P Amar, B Toma

1168 related Products with: Specificity of three ELISA-gE kits for screening pig meat for antibodies to Aujeszky's disease.

100 ml100 TESTS1 ml0.2 mg0.2 mg0.1 mg25 µg250 ml0.25 mg0.1 mg1 LITRE96T

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#12553581   // To Up

Diagnosis of canine parvovirus by rapid immunomigration on a membrane.

Rapid immunomigration on a membrane was applied to the diagnosis of canine parvovirus (CPV) in 128 samples of faeces containing four strains of parvovirus (two CPV-2a strains, including one vaccine strain, and two CPV-2b strains). The results were compared with the results of haemagglutination and ELISA sandwich techniques. The new test was quick and easy to use, and made it possible to identify both the CPV-2a and CPV-2b strains. Its detection thresholds per gram of faeces corresponded to specific haemagglutination titres of between 320 and 640 and a virus titre of between 10(4) and 10(5) CCID50 (dose required to infect 50 per cent of cell cultures).
A Lacheretz, C Laperrousaz, A Kodjo, N Brajon, D Crevat, S Guillossou

2982 related Products with: Diagnosis of canine parvovirus by rapid immunomigration on a membrane.

1 mg0.25 mg0.1 mg 96 Tests 0.1 mg4 Membranes/Box1 mL1 kit2 Membrane supply2 mL0.1 mg32-50 Sample Kit

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