Product name :Mouse AREG ELISA ELISA

Catalog Number :ELM-AR-001

Quantity :One 96-Well Strip Microplate Kit

Price :362 Eur

Pay now with :

Supplier :Ray Biotech

Product Features

Sensitivity
2 pg/ml
Recommended Dilution (serum & plasma)
2 fold
Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Target Name/s

AR

Species Detected

Mouse

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Specificity

The antibody pair provided in this kit recognizes mouse Amphiregulin.

Storage/Stability

May be stored for up to 6 months at 2° to 8°C from the date of shipment. Standard (recombinant protein) should be stored at -20°C or -80°C (recommended at -80°C) after reconstitution. Opened Microplate Wells or reagents may be store for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Note: the kit can be used within one year if the whole kit is stored at -20°C.
 
 
 
 

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY. Not for diagnostic or therapeutic use.

 

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ELM-Epiregulin-001 Mouse Epiregulin ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 498 ELM-Epiregulin-001 Mouse Epiregulin ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 498€ Pub
ELM-ESelectin-001 Custom Mouse E-Selectin ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 542 ELM-ESelectin-001 Custom Mouse E-Selectin ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 542€ Pub
ELM-FcgRIIB-001 Mouse Fc-gamma RIIB ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 498 ELM-FcgRIIB-001 Mouse Fc-gamma RIIB ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 498€ Pub
ELM-FetuinA-001 Custom Mouse Fetuin A ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 542 ELM-FetuinA-001 Custom Mouse Fetuin A ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 542€ Pub
ELM-Fractalkine-001 Mouse Fractalkine ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 362 ELM-Fractalkine-001 Mouse Fractalkine ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 362€ Pub
ELM-Galectin3-001 Mouse Galectin-3 ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 498 ELM-Galectin3-001 Mouse Galectin-3 ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 498€ Pub
ELM-Galectin7-001 Mouse Galectin-7 ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 362 ELM-Galectin7-001 Mouse Galectin-7 ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 362€ Pub
ELM-GAS1-001 Custom Mouse GAS1 ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 542 ELM-GAS1-001 Custom Mouse GAS1 ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 542€ Pub
ELM-GAS6-001 Mouse GAS6 ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 362 ELM-GAS6-001 Mouse GAS6 ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 362€ Pub
ELM-GITR-001 Custom Mouse GITR ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 542 ELM-GITR-001 Custom Mouse GITR ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 542€ Pub
ELM-GITRL-001 Custom Mouse GITR L ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 542 ELM-GITRL-001 Custom Mouse GITR L ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 542€ Pub
ELM-gp130-001 Custom Mouse gp130 ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 542 ELM-gp130-001 Custom Mouse gp130 ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 542€ Pub
ELM-H60-001 Custom Mouse H60 ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 542 ELM-H60-001 Custom Mouse H60 ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 542€ Pub
ELM-HAI1-001 Custom Mouse HAI-1 ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 542 ELM-HAI1-001 Custom Mouse HAI-1 ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 542€ Pub
ELM-HGF-001 Mouse HGF ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 362 ELM-HGF-001 Mouse HGF ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 362€ Pub
ELM-HGF-001C Mouse HGF ELISA (For Lysates) ELISA Ray Biotech One 96-Well Strip Microplate Kit 362 ELM-HGF-001C Mouse HGF ELISA (For Lysates) ELISA Ray Biotech One 96-Well Strip Microplate Kit 362€ Pub
ELM-HGFR-001 Custom Mouse HGF R ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 542 ELM-HGFR-001 Custom Mouse HGF R ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 542€ Pub
ELM-IFNgamma-001 Mouse IFN-gamma ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 362 ELM-IFNgamma-001 Mouse IFN-gamma ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 362€ Pub
ELM-IFNgamma-001C Mouse IFN-gamma ELISA (For Lysates) ELISA Ray Biotech One 96-Well Strip Microplate Kit 362 ELM-IFNgamma-001C Mouse IFN-gamma ELISA (For Lysates) ELISA Ray Biotech One 96-Well Strip Microplate Kit 362€ Pub
ELM-IFNgammaR1-001 Custom Mouse IFN-gamma R1 ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 542 ELM-IFNgammaR1-001 Custom Mouse IFN-gamma R1 ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 542€ Pub
ELM-IGFI-001 Mouse IGF-1 ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 362 ELM-IGFI-001 Mouse IGF-1 ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 362€ Pub
ELM-IGFII-001 Mouse IGF-2 ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 362 ELM-IGFII-001 Mouse IGF-2 ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 362€
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ELM-IL12P40/70-001 Mouse IL-12 p40 70 ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 362 ELM-IL12P40/70-001 Mouse IL-12 p40 70 ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 362€
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ELM-IL1alpha-001 Mouse IL-1 alpha ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 362 ELM-IL1alpha-001 Mouse IL-1 alpha ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 362€
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ELM-IL1beta-001 Mouse IL-1 beta ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 362 ELM-IL1beta-001 Mouse IL-1 beta ELISA ELISA Ray Biotech One 96-Well Strip Microplate Kit 362€
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ELM-IL1beta-001C Mouse IL-1 beta ELISA (For Lysates) ELISA Ray Biotech One 96-Well Strip Microplate Kit 362 ELM-IL1beta-001C