DESCRIPTION

CHOLESTEROL concentrations in High-Density Lipoprotein (HDL)
and Low-Density (LDL)/Very-Low-Density (VLDL) Lipoproteins are
strong predictors for coronary heart disease. Functional HDL offers
protection by removing cholesterol from cells and atheroma. Higher
concentrations of LDL and lower concentrations of functional HDL are
strongly associated with cardiovascular disease due to higher risk of
atherosclerosis. The balances between high- and low-density
lipoproteins are solely genetically determined, but can be changed by
medications, food choices and other factors.
Simple, direct and automation-ready procedures for measuring HDL
and LDL/VLDL concentrations are very desirable. BioAssay Systems'
HDL and LDL/VLDL quantification kit is based on our improved PEG
precipitation method in which HDL and LDL/VLDL are separated, and
cholesterol concentrations are determined using a single Working
Reagent that combines cholesterol ester hydrolysis, oxidation and
color reaction in one step. The color intensity of the reaction product at
570nm or fluorescence intensity at lem/ex = 585/530nm is directly
proportional to total cholesterol concentration in the sample.
 

APPLICATIONS

Direct Assays: HDL and LDL/VLDL cholesterol in serum samples.
Pharmacology: evaluation of drugs on cholesterol metabolism.
 

KEY FEATURES

Sensitive and accurate. Linear detection range in 96-well plate: 1 to
100 mg/dL cholesterol for colorimetric assays and 0.2 to 10 mg/dL for
fluorimetric assays.
Convenient. Room temperature assay. No 37°C heater is needed.
 

KIT CONTENTS

(100 assays in 96-well plates)
PBS: 2 × 1.5 mL Precipitation Reagent: 1.5 mL
Assay Buffer: 20 mL Enzyme Mix: 120 uL
Dye Reagent: 120 μL Standard: 1 mL 300mg/dL cholesterol

Storage conditions 

Store PBS and Precipitation Reagent at room
temperature and other reagents at -20°C. Shelf life: 6 months after
receipt.
 

Precautions: reagents are for research use only. Normal precautions
for laboratory reagents should be exercised while using the reagents.
Please refer to Material Safety Data Sheet for detailed information.