APOBEC3G

Human

This product detects a band of approximately 46kDa in Human CaCO-2 cell lysates.

This product recognizes an epitope within the N-terminal region (NT) of APOBEC3G (apolipoprotein B mRNA-editing, enzyme-catalytic, polypeptide-like 3G), a 46kDa multi-isoform endogenous deoxycytidine deaminase, which plays a major role in the defence of T lymphocytes against retroviral infection.

The incorporation of APOBEC3G into HIV-1 virion DNA, results in the deamination of dC to dU in minus-strand viral DNA during reverse transcription, thereby generating G to A hypermutations in the newly-synthesised viral DNA. The effect of APOBEC3G is counteracted by the HIV protein Vif, thought to induce the degradation of APOBEC3G through a ubiquitin-dependant proteasomal pathway.

This product also detects the APOBEC3F isoform (apolipoprotein B mRNA-editing, enzyme-catalytic, polypeptide-like 3F).

Store at +4 °C or at -20 °C if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 18 months after receipt.

IgG concentration 1.0mg/ml

Purified IgG prepared by affinity chromatography