Kit Summary:
• Detection method- Fluorescence microscopy, Flow cytometry and fluorescence plate reader (Ex. = 485 nm and Em. = 535 nm)
• Sample type- Live cells
• Species reactivity- Mammalian
• Kit size- Convenient sizes (25 and 100 assays)
• Applications- Sensitive detection of activated caspase-9 in living cells.

Features & Benefits:
• Simple one-step procedure; takes only 1-2 hours
• Fast and convenient
• The FITC label allows for direct detection of the activated caspases in apoptotic cells

Kit components:
• FITC-LEHD-FMK
• Wash Buffer
• Z-VAD-FMK

Description:
Activation of caspases plays a central role in apoptosis. The CaspGLOW™ Fluorescein Active Caspase-9 Staining Kit provides a convenient means for sensitive detection of activated caspase-9 in living cells. The assay utilizes the caspase-9 inhibitor LEHD-FMK conjugated to FITC (FITC-LEHD-FMK) as a fluorescent marker. FITC-LEHD-FMK is cell permeable, nontoxic, and irreversibly binds to activated caspase-9 in apoptotic cells.

Storage Conditions:
-20°C

Shipping Conditions:
gel pack

USAGE: For Research Use Only! Not For Use in Humans.