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#34112437   2021/05/24 To Up

Signal-on photoelectrochemical immunoassay mediated by the etching reaction of oxygen/phosphorus co-doped g-CN/AgBr/MnO nanohybrids.

We designed a signal-on photoelectrochemical (PEC) immunoassay for the sensitive monitoring of prostate-specific antigen (PSA) based on the etching reaction of hydrogen peroxide (HO) toward oxygen/phosphorus co-doped graphitic CN/AgBr/MnO nanosheets (OP-g-CN/AgBr/MnO). Initially, glucose oxidase (GO)-labeled detection antibodies were introduced into the capture antibody-coated microplate with a sandwich-type immunoreaction in the presence of PSA. Then, the as-generated HO from the decomposition of glucose by GO etched the manganese dioxide (MnO) nanosheets into manganese ions (Mn), thereby causing the exposure of the underlying OP-g-CN/AgBr. Meanwhile, HO could be also used as an electron scavenger, and restrain the recombination of the electron-hole pairs of OP-g-CN/AgBr. Two advantages of HO enhanced the photocurrent synergistically. Under optimum conditions, the PEC immunoassay showed high sensitivity toward target PSA within a dynamic working range of 0.05-50 ng mL with a limit of detection of 17 pg mL. In addition, our system possessed high specificity, favorable selectivity, and good stability. Relative to commercialized PSA ELISA kits, the accuracy of our strategy was acceptable. More importantly, our strategy can be easily extended to screen other biomarkers by controlling the corresponding antibodies.
Zijian Lv, Ling Zhu, Zipeng Yin, Meijin Li, Dianping Tang

2329 related Products with: Signal-on photoelectrochemical immunoassay mediated by the etching reaction of oxygen/phosphorus co-doped g-CN/AgBr/MnO nanohybrids.

2000 Units4X1.25ml; 200 reactions (30 Reactions100 reactions48 reactions12x5 reactions /200 react 25 ml 1 kit1x96 well plate200 Reactions50 IU

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#34110461   2021/06/10 To Up

Silicon, an important exposure marker in vivo in silicosis research.

The degree of silicosis exposure is closely related to the progress of silicosis. At present, we use animal and human studies to explore whether silicon can be an important exposure marker in the development of silicosis.
Hongli Wang, Jie Cui, Xiaohui Hao, Lingli Guo, Jinyuan Zhao, Ruimin Wang, Heliang Liu

1727 related Products with: Silicon, an important exposure marker in vivo in silicosis research.

1 mg48 samples100 100 μg100 μg100 μg100 μg1 Set1 Set

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#34109517   2021/06/09 To Up

Blocking SphK1/S1P/S1PR1 Signaling Pathway Alleviates Lung Injury Caused by Sepsis in Acute Ethanol Intoxication Mice.

Acute ethanol intoxication increases the risk of sepsis and aggravates the symptoms of sepsis and lung injury. Therefore, this study aimed to explore whether sphingosine kinase 1 (SphK1)/sphingosine-1-phosphate (S1P)/S1P receptor 1 (S1PR1) signaling pathway functions in lung injury caused by acute ethanol intoxication-enhanced sepsis, as well as its underlying mechanism. The acute ethanol intoxication model was simulated by intraperitoneally administering mice with 32% ethanol solution, and cecal ligation and puncture (CLP) was used to construct the sepsis model. The lung tissue damage was observed by hematoxylin-eosin (H&E) staining, and the wet-to-dry (W/D) ratio was used to evaluate the degree of pulmonary edema. Inflammatory cell counting and protein concentration in bronchoalveolar lavage fluid (BALF) were, respectively, detected by hemocytometer and bicinchoninic acid (BCA) method. The levels of tumor necrosis factor (TNF)-α, interleukin (IL)-6, IL-1β, and IL-18 in BALF were detected by their commercial enzyme-linked immunosorbent assay (ELISA) kits. The myeloperoxidase (MPO) activity and expression of apoptosis-related proteins and SphK1/S1P/S1PR1 pathway-related proteins were, respectively, analyzed by MPO ELISA kit and Western blot analysis. The cell apoptosis in lung tissues was observed by TUNEL assay. Acute ethanol intoxication (EtOH) decreased the survival rate of mice and exacerbated the lung injury caused by sepsis through increasing pulmonary vascular permeability, neutrophil infiltration, release of inflammatory factors, and cell apoptosis. In addition, EtOH could activate the SphK1/S1P/S1PR1 pathway in CLP mice. However, PF-543, as a specific inhibitor of SphK1, could partially reverse the deleterious effects on lung injury of CLP mice. PF-543 alleviated lung injury caused by sepsis in acute ethanol intoxication rats by suppressing the SphK1/S1P/S1PR1 signaling pathway.
Liang Chen, Lingling Li, Yong Song, Tangfeng Lv

2472 related Products with: Blocking SphK1/S1P/S1PR1 Signaling Pathway Alleviates Lung Injury Caused by Sepsis in Acute Ethanol Intoxication Mice.

2 Pieces/Box2 Pieces/BoxInhibitors2 Pieces/Box2 Pieces/Box8 inhibitors2 Pieces/Box2 Pieces/Box14 inhibitors2 Pieces/Box7 inhibitors2 Pieces/Box

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#34109437   2021/06/10 To Up

Resveratrol affects the expression of uric acid transporter by improving inflammation.

Resveratrol (RSV), a polyphenol, non‑flavonoid plant‑derived antitoxin, ameliorates hyperuricemia and kidney inflammation. The present study aimed to establish a model of high‑fat diet (HFD)‑induced insulin resistance (IR) and to determine the specific mechanism of RSV to improve kidney inflammation and reduce uric acid (UA). C57BL/6J mice were fed a HFD for 12 weeks and their glucose tolerance was evaluated by intraperitoneal glucose tolerance testing. The mice were then administered RSV for 6 weeks, and blood and kidney samples were collected. Serum UA and insulin concentrations were determined using ELISA kits. Hematoxylin and eosin, periodic acid‑Schiff and Masson staining were performed to observe the pathological changes of the kidney, and electron microscopy was used to observe changes in the kidney ultrastructure. The renal concentrations of interleukin (IL)‑6, IL‑18, IL‑1β and tumor necrosis factor‑α (TNF‑α) were measured using ELISA kits, and western blotting evaluated changes in the protein expression levels of various indicators. RSV significantly ameliorated HFD‑induced IR and reduced blood UA levels. Long‑term IR can lead to lipid deposition, glycogen accumulation, inflammatory damage and fibrotic changes in the kidney of mice. This leads to a significant increase in the expression of UA transport‑related proteins, an increase in UA reabsorption and an increase in blood UA levels. Notably, RSV intervention was able to reverse this process. The effect of RSV may be achieved by inhibiting the NOD‑like receptor family, pyrin domain‑containing 3 (NLRP3) inflammasome and Toll‑like receptor 4 (TLR4)/myeloid differentiation factor 88/nuclear factor‑κB signaling pathway. In conclusion, RSV may improve kidney inflammation through TLR4 and NLRP3 signaling pathways, and reduce the expression of UA transporter proteins in the kidney of insulin‑resistant mice, thereby reducing blood UA levels.
Xuemei Zhang, Qian Nie, Zhimei Zhang, Jing Zhao, Fengxiao Zhang, Chao Wang, Xing Wang, Guangyao Song

2352 related Products with: Resveratrol affects the expression of uric acid transporter by improving inflammation.

25 G240/kit480/kit250400/kit250 assays100 assays 1 G 1 G 100 Slides 5 G

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#34103286   // To Up

Seroprevalence of and in Cattle in Oğuzlar Region

This study aimed to investigate the seroprevalence of and in cattle in the Oğuzlar district of Çorum province.
Dilek Kula, Sami Gökpınar

1354 related Products with: Seroprevalence of and in Cattle in Oğuzlar Region

100 μg100 μg100 μg100 μg100 μg100 μg100 μg100 μg100 μg100 μg100 μg100 μg

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#34100366   // To Up

Effects of vertebral fusion on levels of pro-inflammatory and catabolic mediators in a rabbit model of intervertebral disc degeneration.

The aim of this study was to explore the alterations in levels of pro-inflammatory and catabolic mediators following vertebral fusion in a rabbit model of intervertebral disc degeneration.
Davut Dumanlıdağ, Didem Keleş, Gülgün Oktay, Can Koşay

2226 related Products with: Effects of vertebral fusion on levels of pro-inflammatory and catabolic mediators in a rabbit model of intervertebral disc degeneration.

100ug5ug100ug200ul20 100ug Lyophilized100ug100ug100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug

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#34100364   // To Up

Serum progranulin to TNF-α ratio in patients with gonarthrosis.

Progranulin (PGRN) is a growth factor that has antiinflammatory, immunosuppressive, and chondroprotective effects. It blocks Tumor Necrosis Factor-α (TNF-α) signal pathway by binding its receptor. Recently, it has been claimed that PGRN may be overexpressed in patients with Osteoarthritis (OA). However, these patients tend to be obese and obesity also may be one of the factors that affect PGRN levels. The aim of this study was to compare the PGRN levels of patients with Knee OA (KOA) with that of healthy controls by eliminating the effect of obesity and to evaluate PGRN-to-Tumor Necrosis Factor-α (TNF-α) ratio in KOA, both of which were investigated first in literature by this study.
Kadri Yıldız, Fatih Boy

1863 related Products with: Serum progranulin to TNF-α ratio in patients with gonarthrosis.

1 mg96 wells10 ml100 ml100 ml100ml50 ml

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#34097306   2021/06/07 To Up

MiR-221-3p and miR-92a-3p enhances smoking-induced inflammation in COPD.

Smoking is likely to facilitate airway inflammation and finally contributes to chronic obstructive pulmonary disease (COPD). This investigation was intended to elucidate miRNAs that were involved in smoking-induced COPD.
Yahui Shen, Huiyu Lu, Guixian Song

1969 related Products with: MiR-221-3p and miR-92a-3p enhances smoking-induced inflammation in COPD.

50 ul1-8 Sample Kit96 assays4 Sample Kit1 mg4 Membranes/Box8 Sample Kit 100ul16 Arrays/Slide32-50 Sample Kit

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#34095552   2021/05/21 To Up

The effect of redox signaling on extracellular matrix changes in diabetic wounds leading to amputation.

& Objectives: Redox signaling is a critical regulator in the process of wound healing. This signaling pathway can be effective in the development or healing of diabetic ulcers through the ECM.In this study, the structure of extracellular matrix investigated in relation to redox signaling in the tissue of patients with diabetic ulcers that lead to organ amputation.
Mohamad Hadi Saeed Modaghegh, Shirin Saberianpour, Sakineh Amoueian, Jamal Jalili Shahri, Hamidreza Rahimi

1569 related Products with: The effect of redox signaling on extracellular matrix changes in diabetic wounds leading to amputation.

96 tests 1 G100 1mg25 2 Pieces/Box100 ml.

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