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#32491306   2020/06/03 To Up

The different tests for the diagnosis of COVID-19 - A review in Brazil so far.

SARS-CoV-2 is a novel virus from the coronavirus family that emerged in the end of December 2019 in Wuhan, China. The virus is now widespread and causing the current pandemic of COVID-19, a highly pathogenic viral pneumonia, commonly presented with fever and cough, which frequently lead to lower respiratory tract disease with poor clinical outcomes associated with older age and underlying health conditions. Supportive care for patients is typically the standard protocol because no specific effective antiviral therapies have been identified so far. The current outbreak is challenging governments and health authorities all over the world. In here we present a comparison among the current diagnostic tools and kits being used to test Brazilian population.
Ana Flávia Santarine Laureano, Márcia Riboldi

2354 related Products with: The different tests for the diagnosis of COVID-19 - A review in Brazil so far.

100ul20 ug1 500 ml 500 tests

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#32489820   2020/04/21 To Up

Cytokine levels in gingival crevicular fluid samples of patients wearing clear aligners.

The aim of this study was to assess & compare the changes in cytokine levels in GCF samples of patients wearing clear aligners.
Sana Bint Aziz, Gurkeerat Singh

2541 related Products with: Cytokine levels in gingival crevicular fluid samples of patients wearing clear aligners.

16 Arrays/Slide16 Arrays/Slide16 Arrays/Slide16 Arrays/Slide16 Arrays/Slide16 Arrays/Slide4 Arrays/Slide121roll (50 m)4 Membranes/Box96 samples

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#32487191   2020/06/01 To Up

Shock wave lithotripsy, for the treatment of kidney stones, results in changes to routine blood tests and novel biomarkers: a prospective clinical pilot-study.

The number of patients undergoing shock wave lithotripsy (SWL) for kidney stones is increasing annually, and as such the development of post-operative complications, such as haematuria and acute kidney injury (AKI) following SWL, is likely to increase. The aim of the study was to evaluate changes in routine blood and novel biomarkers following SWL, for the treatment of kidney stones.
Stephen F Hughes, Nathan Jones, Samantha J Thomas-Wright, Joseph Banwell, Alyson J Moyes, Iqbal Shergill

1906 related Products with: Shock wave lithotripsy, for the treatment of kidney stones, results in changes to routine blood tests and novel biomarkers: a prospective clinical pilot-study.



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#32485858   2020/05/29 To Up

Javamide-II Inhibits IL-6 without Significant Impact on TNF-alpha and IL-1beta in Macrophage-Like Cells.

The main aim of this study is to find a therapeutic compound to inhibit IL-6, not TNF-alpha and IL-1beta, in macrophage-like cells, because the high-levels of IL-6 production by macrophages are reported to cause unfavorable outcomes under several disease conditions (e.g., autoimmune diseases, and acute viral infections, including COVID-19). In this study, the potential effects of javamide-II on IL-6, IL-1beta and TNF-alpha productions were determined using their ELISA kits in macrophage-like THP-1 cells. Western blots were also performed using the same cells, to determine its effects on signaling pathways (ERK, p38, JNK, c-Fos, ATF-2, c-Jun and NF-κB p65). At concentrations of 0.2-40 µM, javamide-II inhibited IL-6 production significantly in the THP-1 cells (IC of 0.8 µM) ( < 0.02). However, javamide-II did not inhibit IL-1beta or TNF-alpha productions much at the same concentrations. In addition, the treatment of javamide-II decreased the phosphorylation of p38 without significant effects on ERK and JNK phosphorylations in the THP-1 cells. Furthermore, the p38 inhibition, followed by the reduction of ATF-2 phosphorylation (not c-Fos, c-Jun or NF-κB p65), led to the suppression of IL-6 mRNA expression in the cells ( < 0.02). The data indicate that javamide-II may be a potent compound to inhibit IL-6 production via suppressing the p38 signal pathway, without significant effects on the productions of TNF-alpha and IL-1beta in macrophage-like THP-1 cells.
Jae B Park, Renee Peters, Quynhchi Pham, Thomas T Y Wang

1881 related Products with: Javamide-II Inhibits IL-6 without Significant Impact on TNF-alpha and IL-1beta in Macrophage-Like Cells.

10 ug2ug5ug5ug5ug5ug1 mg100ug5ug100ug100ug

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#32484485   2020/06/02 To Up

Ultrasensitive optofluidic enzyme-linked immunosorbent assay by on-chip integrated polymer whispering-gallery-mode microlaser sensors.

Optical whispering-gallery-mode (WGM) microcavities offer great promise in ultrasensitive biosensors because of their unique ability to enable resonant recirculation of light to achieve strong light-matter interactions in microscale volumes. However, it remains a challenge to develop cost-effective, high-performance WGM microcavity-based biosensing devices for practical disease diagnosis applications. In this paper, we present an optofluidic chip that is integrated with directly-printed, high-quality-factor (Q) polymer WGM microlaser sensors for ultrasensitive enzyme-linked immunosorbent assay (ELISA). Optical 3D μ-printing technology based on maskless ultraviolet lithography is developed to rapidly fabricate high-Q suspended-disk WGM microcavities. After deposition with a thin layer of optical gain material, low-threshold WGM microlasers are fabricated and then integrated together with optical fibres upon a microfluidic chip to achieve an optofluidic device. With flexible microfluidic technology, on-chip, integrated, WGM microlasers are further modified in situ with biomolecules on surface for highly selective biomarker detection. It is demonstrated that such an optofluidic biochip can measure horseradish peroxidase (HRP)-streptavidin, which is a widely used catalytic molecule in ELISA, via chromogenic reaction at the concentration level of 0.3 ng mL-1. Moreover, it enables on-chip optofluidic ELISA of the disease biomarker vascular endothelial growth factor (VEGF) at the extremely low concentration level of 17.8 fg mL-1, which is over 2 orders of magnitude better than the ability of current commercial ELISA kits.
Xia Ouyang, Tong Liu, Yangxi Zhang, Jijun He, Zijian He, A Ping Zhang, Hwa-Yaw Tam

2286 related Products with: Ultrasensitive optofluidic enzyme-linked immunosorbent assay by on-chip integrated polymer whispering-gallery-mode microlaser sensors.

100 U900 tests1000 Units250400 assays100 assays100μg200 assays

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#32479555   2020/06/01 To Up

Expression of high mobility group B1 and toll-like receptor-nuclear factor κB signaling pathway in chronic subdural hematomas.

Chronic subdural hematoma (CSDH) is an angiogenic and inflammatory disease. Toll-like receptors (TLRs) transduce intracellular signals, resulting in the activation of nuclear factor κB (NF-κB), which leads to the production of inflammatory cytokines. High-mobility group box 1 (HMGB1) functions as a mediator of inflammatory responses through TLRs. In this study, we examined the expression of HMGB1 and components of the Toll-like receptor and NF-κB signaling pathways in the outer membrane of CSDH. Eight patients whose outer membrane was successfully obtained during trepanation surgery were included in this study. The expression of TLR4, myeloid differentiation factor 88 (MyD88), interleukin-1 receptor-associated kinase 4 (IRAK4), TNF receptor-associated factor 6 (TRAF6), TGFβ-activated kinase 1 (Tak1), interferon regulatory factors 3 (IRF3), IκB kinase β (IKKβ), IKKγ, IκBε, IκBα, NF-κB/p65 and β-actin was examined by Western blot analysis. The expression of TLR4, NF-κB/p65 and interleukin-6 (IL-6) was also examined by immunohistochemistry. The concentrations of HMGB1 and IL-6 in CSDH fluids were measured using ELISA kits. Above-mentioned molecules were detected in all cases. In addition, TLR4, NF-κB/p65 and IL-6 were localized in the endothelial cells of vessels within CSDH outer membranes. The concentrations of HMGB1 and IL-6 in CSDH fluids were significantly higher than that in the CSF and serum. There existed a correlation between the concentrations of HMGB1 and IL-6 in CSDH fluids. Our data suggest that HMGB1 in CSDH fluids produces the inflammatory cytokine IL-6 in endothelial cells through the Toll-like receptor and NF-κB signaling pathways. Anti-HMGB1 therapy might be a useful method to treat the growth of CSDH.
Koji Osuka, Yasuo Watanabe, Nobuteru Usuda, Kenichiro Iwami, Shigeru Miyachi, Masakazu Takayasu

2045 related Products with: Expression of high mobility group B1 and toll-like receptor-nuclear factor κB signaling pathway in chronic subdural hematomas.

2 Pieces/Box2 Pieces/Box2 Pieces/Box2 Pieces/Box2 Pieces/Box2 Pieces/Box2 Pieces/Box2 Pieces/Box2 Pieces/Box2 Pieces/Box

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#32476376   // To Up

[Edaravone has a protective role in a mouse model of pulmonary oxygen toxicity].

To find if edaravone can play a protective role in a mouse model of pulmonary oxygen toxicity and explore the intervention mechanism.
Xiao-Chen Bao, Yi-Qun Fang, Jun Ma, Fang-Fang Wang

1787 related Products with: [Edaravone has a protective role in a mouse model of pulmonary oxygen toxicity].

1 mg100 ug100ug4 Membranes/Box1 mg0.2 mg100 μg4 Sample Kit100ug Lyophilized32-50 Sample Kit1 mg1 mg

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#32472395   2020/05/29 To Up

High Levels of Il-19 in Patients with Chronic Inflammatory Demyelinating Polyneuropathy.

Immune-mediated neuropathies include some specific types such as acute and chronic inflammatory demyelinating polyneuropathy (AIDP and CIDP). Previous studies have demonstrated abnormal cellular or humoral immune responses in these conditions. Although aberrant regulation of several cytokines have been reported in AIDP and CIDP, the significance of interleukin 19 (IL-19) in these conditions have not been elucidated yet. In the current study, we assessed serum levels of IL-19 in 12 CIDP patients (female/male ratio, 4/8), 9 AIDP patients (female/male ratio, 3/6), and 27 normal subjects (female/male ratio. 8/19) using commercial ELISA kits. Notably, we detected higher levels of this cytokine in CIDP patients (136.4 ± 8.57 ng/l) compared with both AIDP patients (93.89 ± 2.26 ng/l) and controls (83.78 ± 1.72 ng/l). However, the differences between AIDP patients and controls were not significant. The current study demonstrates the role of IL-19 in the pathogenesis of CIDP and potentiates this cytokine as a biomarker for this condition.
Somayeh Sangsefidi, Soudeh Ghafouri-Fard, Alireza Komaki, Mehrdokht Mazdeh, Mohammad Taheri, Mohammad Mahdi Eftekharian

2068 related Products with: High Levels of Il-19 in Patients with Chronic Inflammatory Demyelinating Polyneuropathy.

4 Membranes/Box2 Pieces/Box4 Membranes/Box2 Pieces/Box4 Arrays/Slide2 Pieces/Box4 Membranes/Box2 Pieces/Box

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#32468054   2020/05/22 To Up

Protective effect of microRNA‑340‑5p against oxygen‑glucose deprivation/reperfusion in PC12 cells through targeting neuronal differentiation 4.

The expression levels of microRNA (miR)‑340‑5p are reportedly decreased in the peripheral blood during acute ischemic stroke; however, the direct effect and mechanism of action of miR‑340‑5p in ischemic stroke remains largely unknown. The present study aimed to investigate the effects of miR‑340‑5p, and its mechanism of action, on PC12 cells following oxygen‑glucose deprivation/reperfusion (OGD/R) induction. OGD/R‑induced PC12 cells served as the cellular model and subsequently, mRNA expression levels of miR‑340‑5p and neuronal differentiation 4 (Neurod4) were analyzed using reverse transcription‑quantitative PCR. Tumor necrosis factor‑α, interleukin (IL)‑1β and IL‑6 expression levels were detected using ELISA kits, and flow cytometry was used to determine the rate of cellular apoptosis. In addition, a nitric oxide (NO) synthase activity assay kit was used to detect NO levels and a NADPH assay kit was used to measure NADPH levels. Western blotting was also performed to analyze protein expression levels of bax, bcl‑2, cleaved caspase 3 and phosphorylated endothelial NOS (eNOS), and the target gene of miR‑340‑5p was predicted using TargetScan software and verified using a dual‑luciferase reporter assay. The expression levels of miR‑340‑5p were decreased in PC12 cells following OGD/R induction and Neurod4 was identified as a target gene of miR‑340‑5p. In addition, miR‑340‑5p overexpression reduced inflammation, apoptotic rate, NO production and NADPH levels, in addition to increasing eNOS expression in PC12 cells following OGD/R induction. Notably, the overexpression of Neurod4 reversed the aforementioned effects of miR‑340‑5p on PC12 cells following OGD/R induction. In conclusion, the findings of the present study suggested that miR‑340‑5p may protect PC12 cells against OGD/R through targeting Neurod4, which could provide important implications for the treatment of ischemia‑reperfusion injury based on miR‑340‑5p expression levels in vivo.
Juan Wang, Ganzhe Liu

1691 related Products with: Protective effect of microRNA‑340‑5p against oxygen‑glucose deprivation/reperfusion in PC12 cells through targeting neuronal differentiation 4.

96 assays5 x 50 ug10 rxns100 μg10 ug1.00 flask1x10e7 cells2 Pieces/Box50 ug

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