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#32473570   2020/05/27 To Up

Recombinant human ulinastatin improves immune dysfunction of dendritic cells in septic mice by inhibiting endoplasmic reticulum stress-related apoptosis.

Urinary trypsin inhibitor (UTI), also known as ulinastatin, has been reported to protect multiple organs against inflammation- and/or injury-induced dysfunction. In the present study, we aimed to investigate the immunomodulation effects of a recombinant human ulinastatin (urinary trypsin inhibitor, UTI) (rhUTI) on splenic dendritic cells (DCs) in cecal ligation and puncture (CLP)-induced septic mice. CLP mice were treated with rhUTI intramuscularly at 0, 12, and 24 h after procedure. Splenic CD11c DCs were isolated and accessed with flow cytometry for apoptotic or phenotypic analysis. Protein markers and cytokines were determined with Western blotting or ELISA. Treatment with rhUTI could markedly upregulate levels of costimulatory molecules (CD80, CD86) and MHC-II on surface of the splenic DC in CLP mice. The apoptotic rate of splenic DCs was decreased in CLP mice after rhUTI treatment. The survival rate of septic mice was increased after treatment with rhUTI. In addition, protein level of markers in endoplasmic reticulum stress (ERS)-related apoptotic pathways (including GRP78, caspase-12, and CHOP) were obviously down-regulated in the rhUTI-treated group when compared with the CLP group. These results indicate that rhUTI protects CLP-induced sepsis in mice by improving immune response of splenic DCs and inhibiting the excessive ERS-mediated apoptosis.
An-Long Qi, Yao Wu, Ning Dong, Yan-Fen Chai, Xiao-Mei Zhu, Yong-Ming Yao

1816 related Products with: Recombinant human ulinastatin improves immune dysfunction of dendritic cells in septic mice by inhibiting endoplasmic reticulum stress-related apoptosis.

1 mg10 ug 100ul100 ug10 25 mg 100 UG2 2 5 10 25

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#32473368   2020/05/27 To Up

Dingkun pill replenishes diminished ovarian reserve through protection of primordial follicle pool by modulating the PI3K/AKT/mTOR signaling pathway.

Diminished ovarian reserve (DOR) can lead to poor fertility and shorten the reproductive lifespan of female. The Dingkun Pill (DKP), a traditional Chinese-patented medication has been an integral part of Chinese treatment for centuries for the management of gynecological diseases. Relevant clinical studies have shown that DKP is able to protect against DOR, however, its mechanism of action is not yet fully known.
Kun Ma, Yanxia Chen, Xiaodi Fan, Yuan Yuan, Kaili Wang, Caidie Tian, Min Li

1633 related Products with: Dingkun pill replenishes diminished ovarian reserve through protection of primordial follicle pool by modulating the PI3K/AKT/mTOR signaling pathway.

2 Pieces/Box1.5 x 10^6 cells5mg2 Pieces/BoxInhibitors2 Pieces/Box5mg2 Pieces/Box5mg2 Pieces/Box1.5x10(6) cells50mg

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#32473361   2020/05/27 To Up

Immunogenicity and protective efficacy of Yersinia ruckeri lipopolysaccharide (LPS), encapsulated by alginate-chitosan micro/nanoparticles in rainbow trout (Oncorhyncus mykiss).

Considering the many advantages of oral vaccines in aquaculture, several studies have been conducted in this area recently. In this study, immunization and protective power of the oral vaccine of Yersinia ruckeri encapsulated with Alginate-Chitosan micro/nanoparticles were evaluated in rainbow trout. For this purpose, 720 juvenile rainbow trout (9 ± 1.8 g) were divided into 8 groups in three replications (30 fish each) as follows: Groups A, B and C, were immunized with Yersinia ruckeri lipopolysaccharide (LPS), LPS+Formalin Killed Cells (FKC) and FKC alone, groups D, E, and F were immunized with encapsulated LPS, LPS+FKC and FKC, respectively. The G and H groups considered as encapsulated and non-encapsulated control, respectively. Micro/nanoencapsulation with alginate-chitosan was performed by internal emulsification method and vaccination were conductrd in the first and third weeks via oral route. Sampling was performed on days 0, 30, and 60 of experiment. Anti Y. ruckeri antibody titer in serum, intestine and skin mucus were measured via ELISA method. Non-specific immune response including: serum lysozyme, complement, bactericidal and respiratory burst activity, serum protein and globulin level, as well as white blood cell count were compared among the groups. The expression of IgT gene in the intestine and TCR gene in the anterior kidney were also investigated. At the end of the study, the fish were challenged with Y. ruckeri through immerssion and intraperitoneal routs and the relative survival rate was evaluated. Result showed that the antibody level in serum, skin and intestine was significantly higher in group E and F than control groups (P < 0.05), meanwhile serum, skin and intestine antibody level in all vaccinated groups were significantly (P < 0.01) higher in day 30 and 60 compare to zero day. Non-specific immunity factors including: serum lysozyme, complement, and respiratory burst activity as well as WBC, protein and Globulin level were significantly higher in E and F groups not only in day 30 but also in day 60 of experiment (P < 0.05). Cumulative mortality following injection and bath challenge were significantly (P = 0.004) lower (35%-45%) in groups E and F compare to control group (80%). The IgT and TCR gene expression in groups D, E and F were significantly higher (P < 0.05) than control group. Highest upregulation of IgT and TCR gene expression in vaccinated groups were seen at day 30 and 60 respectively which were significantly (P < 0.001) higher than day zero. Generally, it can be concluded that nano/micronanoencapsulation of Y. ruckeri FKC+LPS with chitosan-alginate, not only increases protective efficacy of oral vaccine, but improves specific and non-specific immune responses in rainbow trout.
Zahra Tulaby Dezfuly, Mojtaba Alishahi, Masoud Ghorbanpoor, Mohammad Reza Tabandeh, Mehrzad Mesbah

1551 related Products with: Immunogenicity and protective efficacy of Yersinia ruckeri lipopolysaccharide (LPS), encapsulated by alginate-chitosan micro/nanoparticles in rainbow trout (Oncorhyncus mykiss).

1 Set100 μg1 Set1 mg1 Set20000 Units5ug5ug

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#32473121   2020/05/27 To Up

Mitochondrial DNA methylation misleads global DNA methylation detected by antibody-based methods.

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1179 related Products with: Mitochondrial DNA methylation misleads global DNA methylation detected by antibody-based methods.

96 assays 100ul100ug Lyophilized100ug100ug100ug Lyophilized100ug100ug Lyophilized100ug100ug Lyophilized

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#32473021   2020/05/30 To Up

Performance of SARS-CoV-2 antibody assays in different stages of the infection: Comparison of commercial ELISA and rapid tests.

We comparatively assessed sensitivities and specificities of four commercial ELISAs and two rapid tests in 77 patients with PCR confirmed SARS-CoV-2 infection, grouped by intervals since symptom onset. While test sensitivities were low (<40%) within the first 5 days post disease onset, IgM-, IgA- and total antibody-ELISAs increased in sensitivity to >80% between the 6th and 10th day post symptom onset. The evaluated tests (including IgG and rapid tests) provided positive results in all patients at or after the 11th day post onset of disease. Specificities of the ELISAs were 83% (IgA), 98% (IgG) and 97% (IgM and total antibodies).
M Traugott, S W Aberle, J H Aberle, H Griebler, M Karolyi, E Pawelka, E Puchhammer-Stöckl, A Zoufaly, L Weseslindtner

1905 related Products with: Performance of SARS-CoV-2 antibody assays in different stages of the infection: Comparison of commercial ELISA and rapid tests.

96 tests96 tests96 tests96 assays100ug Lyophilized96 tests500 tests100ug Lyophilized96 tests200ul

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