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Search results for: Enhancer

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#34524943   2021/09/15 To Up

Increased alveolar epithelial TRAF6 via autophagy-dependent TRIM37 degradation mediates particulate matter-induced lung metastasis.

Epidemiological and clinical studies have shown that exposure to particulate matter (PM) is associated with an increased incidence of lung cancer and metastasis. However, the underlying mechanism remains unclear. Here, we demonstrated the central role of PM-induced neutrophil recruitment in promoting lung cancer metastasis. We found that reactive oxygen species (ROS)-mediated alveolar epithelial macroautophagy/autophagy was essential for initiating neutrophil chemotaxis and pre-metastatic niche formation in the lungs in response to PM exposure. During PM-induced autophagy, the E3 ubiquitin ligase TRIM37 was degraded and protected TRAF6 from proteasomal degradation in lung epithelial cells, which promoted the NFKB-dependent production of chemokines to recruit neutrophils. Importantly, ROS blockade, autophagy inhibition or TRAF6 knockdown abolished PM-induced neutrophil recruitment and lung metastasis enhancement. Our study indicates that host lung epithelial cells and neutrophils coordinate to promote cancer metastasis to the lungs in response to PM exposure and provides ideal therapeutic targets for metastatic progression.: ACTA2/α-SMA: actin alpha 2, smooth muscle, aorta; ATII: alveolar type II; Cho- siRNA: 5'-cholesterol- siRNA; EMT: epithelial-mesenchymal transition; HBE: human bronchial epithelial; HCQ: hydroxychloroquine; MAPK: mitogen-activated protein kinase; NAC: N-acetyl-L-cysteine; NFKB: nuclear factor of kappa light polypeptide gene enhancer in B cells; NS: normal saline; PM: particulate matter; ROS: reactive oxygen species; TRAF6: TNF receptor-associated factor 6; TRIM37: tripartite motif-containing 37.
Jiajun Liu, Shumin Li, Xuefeng Fei, Xi Nan, Yingying Shen, Huiqing Xiu, Stephania A Cormier, Chaojie Lu, Chuqi Guo, Shibo Wang, Zhijian Cai, Pingli Wang

2157 related Products with: Increased alveolar epithelial TRAF6 via autophagy-dependent TRIM37 degradation mediates particulate matter-induced lung metastasis.

1000pcs1 Set100 ug/vial 6 ml Ready-to-use 100μg/vialEach

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#34523124   2021/09/15 To Up

The hazards of genotype imputation in chromosomal regions under selection: A case study using the Lactase gene region.

Although imputation of missing SNP results has been widely used in genetic studies, claims about the quality and usefulness of imputation have outnumbered the few studies that have questioned its limitations. But it is becoming clear that these limitations are real-for example, disease association signals can be missed in regions of LD breakdown. Here, as a case study, using the chromosomal region of the well-known lactase gene, LCT, we address the issue of imputation in the context of variants that have become frequent in a limited number of modern population groups only recently, due to selection. We study SNPs in a 500 bp region covering the enhancer of LCT, and compare imputed genotypes with directly genotyped data. We examine the haplotype pairs of all individuals with discrepant and missing genotypes. We highlight the nonrandom nature of the allelic errors and show that most incorrect imputations and missing data result from long haplotypes that are evolutionarily closely related to those carrying the derived alleles, while some relate to rare and recombinant haplotypes. We conclude that bias of incorrectly imputed and missing genotypes can decrease the accuracy of imputed results substantially.
Aminah T Ali, Anke Liebert, Winston Lau, Nikolas Maniatis, Dallas M Swallow

1344 related Products with: The hazards of genotype imputation in chromosomal regions under selection: A case study using the Lactase gene region.

100 μg1

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#34522931   2021/09/15 To Up

Flavonoids from Pall. fruits prevent high-fat diet-induced obesity and liver injury modulation of the gut microbiota in mice.

Pall. (RDP) fruits are popularly consumed as beverages and healthy food in China because of their various beneficial activities. In particular, flavonoids are one of the major active ingredients of RDP fruits with predominant pharmacological effects. However, the anti-obesity activities of flavonoids from RDP fruits and their regulation effect on the gut microbiota have not been determined. In the present study, the flavonoid-rich extracts (RDPF) were isolated from RDP fruits and their anti-obesity effects were investigated using a high-fat diet (HFD)-induced obese mouse model. The results showed that RDPF intervention significantly inhibited the body weight, liver weight, kidney weight and epididymal adipose tissue weight of HFD-fed mice without affecting the calorie intake. Plasma lipid levels were also significantly lowered by RDPF treatment. Histological examination showed that RDPF supplementation partially recovered HFD-induced hepatic steatosis in the liver. RDPF also prevented oxidative injury of the liver, as evidenced by the altered superoxide dismutase (SOD), catalase (CAT) and malondialdehyde (MDA) levels. The expression levels of CCAAT/enhancer binding protein α (C/EBPα), sterol regulatory element binding protein-1C (SREBP-1C), fatty acid synthase (FAS), acyl-coenzyme A oxidase 1 (ACOX1), peroxisome proliferator-activated receptor (PPARα) and CAT mRNA in the livers of mice were also regulated by RDPF administration. 16S rRNA gene sequence data further indicated that RDPF addition increased the microbial diversity and reshaped the community composition. Intriguingly, RDPF intervention did not exhibit inhibitory tendency toward the ratio of Firmicutes to Bacteroidetes, but markedly decreased the relative abundance of Erysipelotrichaceae. This study provided novel insights into the application of RDPF in the food industry.
Chun-Yan Shen, Yun-Fang Hao, Zhan-Xi Hao, Qiang Liu, Lu Zhang, Cui-Ping Jiang, Jian-Guo Jiang

1357 related Products with: Flavonoids from Pall. fruits prevent high-fat diet-induced obesity and liver injury modulation of the gut microbiota in mice.

96 tests400 ug

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#34522636   2021/02/25 To Up

Menhaden fish oil attenuates postpartum depression in rat model via inhibition of NLRP3-inflammasome driven inflammatory pathway.

Postpartum depression (PPD) is a familiar problem which is associated with about 10-20% of women after child delivery. Fish oil (FO) has a therapeutic potentials to many diseases including mood disorders. However, there is paucity of data on the effects of FO supplementation on PPD rat model. Hence, this study aimed at investigating the potentials of FO in ameliorating depressive-like behaviors in PPD rat by evaluating the involvement of NLRP3-inflammasome.
Nurul Uyun Abdul Aziz, Samaila Musa Chiroma, Mohamad Aris Mohd Moklas, Mohd Ilham Adenan, Amin Ismail, Rusliza Basir, Razana Mohd Ali, Mohamad Taufik Hidayat Bin Baharuldin

1248 related Products with: Menhaden fish oil attenuates postpartum depression in rat model via inhibition of NLRP3-inflammasome driven inflammatory pathway.

2 Pieces/Box2 Pieces/Box5ug2 Pieces/Box48 assays 100 μg2 Pieces/Box2 Pieces/Box5ug

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#34522635   2021/02/15 To Up

Rapid changes of miRNAs-20, -30, -410, -515, -134, and -183 and telomerase with psychological activity: A one year study on the relaxation response and epistemological considerations.

Mental stress represents a pivotal factor in cardiovascular diseases. The mechanism by which stress produces its deleterious effects is still under study, but one of the most explored pathways is inflammation-aging and cell senescence. In this scenario, circulating microRNAs appear to be regulatory elements of the telomerase activity and alternative splicing within the nuclear factor kappa-light-chain-enhancer (NF-κB) network. Anti-stress techniques appeared to be able to slow down the inflammatory and aging processes. As we recently verified, the practice of the relaxation response (RR) counteracted psychological stress and determined favorable changes of the NF-κB, p53, and toll-like receptor-4 (TLR-4) gene expression and in neurotransmitters, hormones, cytokines, and inflammatory circulating microRNAs. We aimed to verify a possible change in the serum levels of six other micro-RNAs of cardiovascular interest, involved in cell senescence and in the NF-κB network (miRNAs -20, -30, -410, -515, -134, and -183), and tested the activity of telomerase in peripheral blood mononuclear cells (PBMCs).
Carlo Dal Lin, Mariela Marinova, Laura Brugnolo, Giorgio Rubino, Mario Plebani, Sabino Iliceto, Francesco Tona

1715 related Products with: Rapid changes of miRNAs-20, -30, -410, -515, -134, and -183 and telomerase with psychological activity: A one year study on the relaxation response and epistemological considerations.

1 ml10 mg 96 Tests 100ul200ul10 mg100ug 5 G100ug25 mg100 mg

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#34522593   2021/04/29 To Up

Oral delivery of proteins and peptides: Challenges, status quo and future perspectives.

Proteins and peptides (PPs) have gradually become more attractive therapeutic molecules than small molecular drugs due to their high selectivity and efficacy, but fewer side effects. Owing to the poor stability and limited permeability through gastrointestinal (GI) tract and epithelia, the therapeutic PPs are usually administered by parenteral route. Given the big demand for oral administration in clinical use, a variety of researches focused on developing new technologies to overcome GI barriers of PPs, such as enteric coating, enzyme inhibitors, permeation enhancers, nanoparticles, as well as intestinal microdevices. Some new technologies have been developed under clinical trials and even on the market. This review summarizes the history, the physiological barriers and the overcoming approaches, current clinical and preclinical technologies, and future prospects of oral delivery of PPs.
Quangang Zhu, Zhongjian Chen, Pijush Kumar Paul, Yi Lu, Wei Wu, Jianping Qi

1464 related Products with: Oral delivery of proteins and peptides: Challenges, status quo and future perspectives.

100ul 25 MG100ul25 mg100 mg200ug10 mg1000 tests100.00 ul100ug200ul

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#34521982   2021/09/14 To Up

Identifying the lungs as a susceptible site for allele-specific regulatory changes associated with type 1 diabetes risk.

Type 1 diabetes (T1D) etiology is complex. We developed a machine learning approach that ranked the tissue-specific transcription regulatory effects for T1D SNPs and estimated their relative contributions to conversion to T1D by integrating case and control genotypes (Wellcome Trust Case Control Consortium and UK Biobank) with tissue-specific expression quantitative trait loci (eQTL) data. Here we show an eQTL (rs6679677) associated with changes to AP4B1-AS1 transcript levels in lung tissue makes the largest gene regulatory contribution to the risk of T1D development. Luciferase reporter assays confirmed allele-specific enhancer activity for the rs6679677 tagged locus in lung epithelial cells (i.e. A549 cells; C > A reduces expression, p = 0.005). Our results identify tissue-specific eQTLs for SNPs associated with T1D. The strongest tissue-specific eQTL effects were in the lung and may help explain associations between respiratory infections and risk of islet autoantibody seroconversion in young children.
Daniel Ho, Denis M Nyaga, William Schierding, Richard Saffery, Jo K Perry, John A Taylor, Mark H Vickers, Andreas W Kempa-Liehr, Justin M O'Sullivan

1121 related Products with: Identifying the lungs as a susceptible site for allele-specific regulatory changes associated with type 1 diabetes risk.

1 LITRE0.2 mg100 ml0.1 mg25 µg0.1 ml100 TESTS25 µg100Tests0.1 mg0.25 mg1 ml

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#34521827   2021/09/14 To Up

Chromatin-based, in cis and in trans regulatory rewiring underpins distinct oncogenic transcriptomes in multiple myeloma.

Multiple myeloma is a genetically heterogeneous cancer of the bone marrow plasma cells (PC). Distinct myeloma transcriptome profiles are primarily driven by myeloma initiating events (MIE) and converge into a mutually exclusive overexpression of the CCND1 and CCND2 oncogenes. Here, with reference to their normal counterparts, we find that myeloma PC enhanced chromatin accessibility combined with paired transcriptome profiling can classify MIE-defined genetic subgroups. Across and within different MM genetic subgroups, we ascribe regulation of genes and pathways critical for myeloma biology to unique or shared, developmentally activated or de novo formed candidate enhancers. Such enhancers co-opt recruitment of existing transcription factors, which although not transcriptionally deregulated per se, organise aberrant gene regulatory networks that help identify myeloma cell dependencies with prognostic impact. Finally, we identify and validate the critical super-enhancer that regulates ectopic expression of CCND2 in a subset of patients with MM and in chronic lymphocytic leukemia.
Jaime Alvarez-Benayas, Nikolaos Trasanidis, Alexia Katsarou, Kanagaraju Ponnusamy, Aristeidis Chaidos, Philippa C May, Xiaolin Xiao, Marco Bua, Maria Atta, Irene A G Roberts, Holger W Auner, Evdoxia Hatjiharissi, Maria Papaioannou, Valentina S Caputo, Ian M Sudbery, Anastasios Karadimitris

2476 related Products with: Chromatin-based, in cis and in trans regulatory rewiring underpins distinct oncogenic transcriptomes in multiple myeloma.

2 Pieces/Box4/120 Packing /sleeve/bo100ug100 μg 1 G

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#34521452   2021/09/14 To Up

Regulatory regions in natural transposable element insertions drive interindividual differences in response to immune challenges in Drosophila.

Variation in gene expression underlies interindividual variability in relevant traits including immune response. However, the genetic variation responsible for these gene expression changes remains largely unknown. Among the non-coding variants that could be relevant, transposable element insertions are promising candidates as they have been shown to be a rich and diverse source of cis-regulatory elements.
Anna Ullastres, Miriam Merenciano, Josefa González

1242 related Products with: Regulatory regions in natural transposable element insertions drive interindividual differences in response to immune challenges in Drosophila.

25 96 wells (1 kit)500 gm.1 mg100 100 96T 1 G1mg

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