Search results for: EpiQuik
#32724383 2020/05/25 To Up
Fat mass and obesity-associated protein promotes the tumorigenesis and development of liver cancer.Liver cancer is the fourth leading cause of cancer-associated mortality worldwide. Statistics indicate that the incidence of liver cancer has been increasing and that its prognosis remains poor. Fat mass and obesity-associated protein (FTO) is a demethylase that is involved in N6-methyladenosine (m6a) RNA modification; however, to the best of our knowledge, its role in tumorigenesis and development of liver cancer remains unknown. In the present study, cell proliferation, colony formation, apoptosis, Transwell and wound healing assays of small interfering (si)RNA-FTO HepG2 cells were performed, and the levels of m6A RNA methylation were assessed. Additionally, the prognostic value of FTO in liver cancer was analyzed using immunohistochemistry analysis. The results from the EpiQuik m6A RNA methylation quantitative assay revealed that knockdown of FTO increased the total m6A methylation level. Notably, FTO promoted the proliferation and migration of liver cancer cells. Additionally, FTO expression was upregulated in patients with liver cancer and was associated with a high Edmondson Grade, which served as an independent prognostic factor for liver cancer. Results from the Kaplan-Meier survival analysis revealed that low expression levels of FTO predicted a good prognosis. The 5-year overall survival of the low FTO expression group was 68% compared with 48% in the high FTO expression group (P=0.077). In conclusion, the present study suggested that FTO regulates the tumorigenesis and development of liver cancer.
Ziqi Ye, Shibing Wang, Wanyuan Chen, Xin Zhang, Jie Chen, Jinying Jiang, Mingshan Wang, Li Zhang, Zixue Xuan
2037 related Products with: Fat mass and obesity-associated protein promotes the tumorigenesis and development of liver cancer.1000 TESTS/0.65ml100ul 5 G 5 G100ul25 mg100ul
#32556427 2020/06/15 To Up
Investigation of promoter methylation of MCPH1 gene in circulating cell-free DNA of brain tumor patients.Despite advanced diagnostic and therapeutic techniques, many brain tumors are still diagnosed at high grades and, therefore finding novel molecular markers may assist in early detection and reducing brain tumors-related mortality rate. Owing to the previous reports on the importance of MCPH1 gene in tumorigenesis, the present study was aimed to study the promoter methylation of MCPH1 gene in paired circulating cell-free DNA (cfDNA) and tumor tissues of brain tumor patients.
Marjan Ghodsi, Mohammadreza Shahmohammadi, Mohammad Hossein Modarressi, Fatemeh Karami
2857 related Products with: Investigation of promoter methylation of MCPH1 gene in circulating cell-free DNA of brain tumor patients.300 units 5 G
#32215717 2020/03/25 To Up
Sulforaphane and iberin are potent epigenetic modulators of histone acetylation and methylation in malignant melanoma.Growing evidence supports that isothiocyanates exert a wide range of bioactivities amongst of which is their capacity to interact with the epigenetic machinery in various cancers including melanoma. Our aim was to characterise the effect of sulforaphane and iberin on histone acetylation and methylation as a potential anti-melanoma strategy.
Melina Mitsiogianni, Dimitrios T Trafalis, Rodrigo Franco, Vasilis Zoumpourlis, Aglaia Pappa, Mihalis I Panayiotidis
2671 related Products with: Sulforaphane and iberin are potent epigenetic modulators of histone acetylation and methylation in malignant melanoma.300 units
#30762097 2019/02/14 To Up
Allyl isothiocyanate regulates lysine acetylation and methylation marks in an experimental model of malignant melanoma.Isothiocyanates (ITCs) are biologically active plant secondary metabolites capable of mediating various biological effects including modulation of the epigenome. Our aim was to characterize the effect of allyl isothiocyanate (AITC) on lysine acetylation and methylation marks as a potential epigenetic-induced anti-melanoma strategy.
Melina Mitsiogianni, Theodora Mantso, Dimitrios T Trafalis, H P Vasantha Rupasinghe, Vasilis Zoumpourlis, Rodrigo Franco, Sotiris Botaitis, Aglaia Pappa, Mihalis I Panayiotidis
2675 related Products with: Allyl isothiocyanate regulates lysine acetylation and methylation marks in an experimental model of malignant melanoma.100 ul
#24433164 2014/01/16 To Up
Aberrant histone methylation in the patients with immune thrombocytopenia.Abstract The aim of the present study was to investigate alterations in histone methylation in patients with primary immune thrombocytopenia (ITP). Global histone H3K4/H3K9 methylation in CD4+ T cells from 35 ITP patients and 15 healthy controls were measured using the EpiQuik(TM) global histone H3K4/H3K9 methylation assay kits. The mRNA expression of SUV39H1, SUV39H2 and EZH2 were detected by real-time quantitative polymerase chain reaction (RT-PCR). The results showed that global histone H3K9 hypomethylation in CD4+ T cells of active ITP, compared with ITP in remission and controls, while the global histone H3K4 methylation were not significantly different between ITP patients and healthy controls. The expression of EZH2 and SUV39H2 were significantly down-regulated in active ITP patients, when compared with ITP in remission and controls. There were not different between ITP patients and controls in the expression SUV39H1. In conclusion, the aberrant histone methylation was involved in the pathogenesis of ITP.
Haifeng Zhao, Feng Xue, Jianfen Xu, Zhi Fang
2228 related Products with: Aberrant histone methylation in the patients with immune thrombocytopenia.300 units48 assays 96 assays 100 ug5mg48 assays 10mg
#23080547 2012/10/19 To Up
Aberrant histone acetylation and methylation levels in woman with endometriosis.To investigate the alterations in histone modifications in woman with endometriosis.
Xia Xiaomeng, Zhao Ming, Ma Jiezhi, Fang Xiaoling
1638 related Products with: Aberrant histone acetylation and methylation levels in woman with endometriosis.300 units96 assays 96 assays 10mg96 assays 5mg48 assays 100 ul96 assays 100 ug
#21715244 // To Up
Abnormal histone modifications in PBMCs from patients with psoriasis vulgaris.Excessive keratinocyte proliferation is thought to be responsible for the formation and development of psoriasis vulgaris. Evidence indicates that epigenetic modifications are associated with aberrant gene expression, however, nothing is known about the status of histone modifications in psoriasis vulgaris. We investigated alterations in histone modifications in patients with psoriasis vulgaris. Global histone H3/H4 acetylation and H3K4/H3K27 methylation in peripheral blood mononuclear cells from 30 psoriatic patients and 20 healthy control subjects were quantiﬁed by the EpiQuik(TM) global histone H3/H4 acetylation and H3K4/H3K27 methylation assay kit. The mRNA levels of 12 members of 3 classes of chromatin modifier genes were measured by real-time quantitative polymerase chain reaction. Compared with normal controls, global histone H4 hypoacetylation was observed in PBMCs from psoriasis vulgaris patients. There was a negative correlation between the degree of histone H4 acetylation and disease activity in patients as measured by PASI. Global levels of H3 acetylation, H3K4/H3K27 methylation did not significantly differ between psoriatic patients and controls. mRNA levels of P300, CBP and SIRT1 were significantly reduced in PBMCs from patients with psoriasis vulgaris compared with healthy controls, while mRNA expression levels of HDAC1, SUV39H1 and EZH2 was significantly increased in psoriatic patients.We conclude that histone modifications are aberrant in the PBMCs of psoriasis vulgaris patients.
Peng Zhang, Yuwen Su, Ming Zhao, Wei Huang, Qianjin Lu
2542 related Products with: Abnormal histone modifications in PBMCs from patients with psoriasis vulgaris.96 assays 10mg100 ul48 assays 96 assays 50mg48 assays 10mg5mg96 assays 5mg
#18398941 2008/04/01 To Up
Abnormal histone modification patterns in lupus CD4+ T cells.To investigate alterations in histone modifications in patients with systemic lupus erythematosus (SLE).
Nan Hu, Xiangning Qiu, Yongqi Luo, Jun Yuan, Yaping Li, Wenzhi Lei, Guiying Zhang, Ying Zhou, Yuwen Su, Qianjin Lu10mg96 tests100 ul100 ul96 tests0.1 mg1 Set100 μg
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