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#35763544   2022/06/28 To Up

Loss of the cleaved-protamine 2 domain leads to incomplete histone-to-protamine exchange and infertility in mice.

Protamines are unique sperm-specific proteins that package and protect paternal chromatin until fertilization. A subset of mammalian species expresses two protamines (PRM1 and PRM2), while in others PRM1 is sufficient for sperm chromatin packaging. Alterations of the species-specific ratio between PRM1 and PRM2 are associated with infertility. Unlike PRM1, PRM2 is generated as a precursor protein consisting of a highly conserved N-terminal domain, termed cleaved PRM2 (cP2), which is consecutively trimmed off during chromatin condensation. The carboxyterminal part, called mature PRM2 (mP2), interacts with DNA and together with PRM1, mediates chromatin-hypercondensation. The removal of the cP2 domain is believed to be imperative for proper chromatin condensation, yet, the role of cP2 is not yet understood. We generated mice lacking the cP2 domain while the mP2 is still expressed. We show that the cP2 domain is indispensable for complete sperm chromatin protamination and male mouse fertility. cP2 deficient sperm show incomplete protamine incorporation and a severely altered protamine ratio, retention of transition proteins and aberrant retention of the testis specific histone variant H2A.L.2. During epididymal transit, cP2 deficient sperm seem to undergo ROS mediated degradation leading to complete DNA fragmentation. The cP2 domain therefore seems to be a key aspect in the complex crosstalk between histones, transition proteins and protamines during sperm chromatin condensation. Overall, we present the first step towards understanding the role of the cP2 domain in paternal chromatin packaging and open up avenues for further research.
Lena Arévalo, Gina Esther Merges, Simon Schneider, Franka Enow Oben, Isabelle Sophie Neumann, Hubert Schorle

2096 related Products with: Loss of the cleaved-protamine 2 domain leads to incomplete histone-to-protamine exchange and infertility in mice.

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#35761708   2022/06/07 To Up

Beneficial effects of Gynostemma pentaphyllum honey paste on obesity via counteracting oxidative stress and inflammation: An exploration of functional food developed from two independent foods rich in saponins and phenolics.

The development of functional foods that possess a combination of biological functions and good sensory properties is an emerging topic in the field of food and function. Gynostemma pentaphyllum (G. pentaphyllum) is widely considered to exert anti-obesity effect owing to its abundant saponins and other bioactive components, but bitter and unacceptable taste limit its utilization. While honey, a natural sweetener, not only has the pleasure sense but is also usually used as the carrier of functional food due to its phenolic oligosaccharide, etc. In the present study, we proposed the preparation method of a G. pentaphyllum honey paste (GH) and its beneficial effects on obese mice. The results showed that GH contented 0.055 mg/g Gypenoside XLIX, 0.01 mg/g Gypenoside A, and 11 kinds of phenolics. It could down-regulate 23.3% of liver TC level, increase serum ALT activity, improve liver tissue damage and epididymal adipocyte hypertrophy than obese mice. Besides, GH regulated enzyme activities such as SOD and GSH to enhance oxidative stress defense and exerted anti-inflammatory activity via IL-6 (52.4%), TNF-α (38.7%), IFN-γ (32%) and NF-κB (28%) genes down-regulation, which also reshaped the gut microbiota structure, exerting anti-obesity effects. More importantly, GH promoted obese mice appetite with orexin-A compared to G. pentaphyllum alone. This study provided a new perspective on the development of G. pentaphyllum functional foods with both good organoleptic performance and obesity therapy.
Sinan Chen, Xinyan Liu, Haoan Zhao, Ni Cheng, Jing Sun, Wei Cao

2866 related Products with: Beneficial effects of Gynostemma pentaphyllum honey paste on obesity via counteracting oxidative stress and inflammation: An exploration of functional food developed from two independent foods rich in saponins and phenolics.

4 Sample Kit25 mg100ug 5 G 5 G4 Arrays/Slide4 Membranes/Box16-22 Sample Kit100ug8 Sample Kit1 g1000 TESTS/0.65ml

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#35758706   2022/06/08 To Up

A Modified Vessel-sparing Microsurgical Vasoepididymostomy.

Microsurgical vasoepididymostomy (MVE) is the main surgical treatment for epididymal obstruction. The vasal vessels are ligated during MVE. However, preserving the vasal vessels during MVE might better simulate the normal physiological structure and be meaningful for patients who have undergone varicocelectomy. Nevertheless, preserving the vasal vessels might elevate the risk of increasing the tension of anastomosis, affecting the patency rate and leading to delayed postoperative bleeding. Therefore, we developed a novel vessel-sparing MVE to make it safer. Here is a summary of the improvements to the procedure. 1) The retrograde dissociation of the vasal vessels on the proximal testicular side was adopted as the main method, and the anterograde dissociation of the vasal vessels on the distal testicular side was adopted as a supplement to dissociate the vasal vessels to be preserved. This improvement ensures the blood supply to the vas deferens that will be used for anastomosis and also provides longer vasal vessels, which reduces the tension of anastomosis. 2) By fixing the vas deferens to be anastomosed and the broken end of the vas deferens, the free vasal vessels get fixed, which resolves the problem of transmission of vas tension to the vasal vessels and reduces the risk of vasal vessel hemorrhage. 3) Dissociation of the vas deferens after opening the tunica vaginalis increases the mobilization of the vas deferens, which also makes the new procedure easier to complete. The evaluation of the outcomes of this new procedure showed that no significant postoperative complications occurred in the patients, and the patency rate was no different from that of the conventional procedure. Therefore, this new, improved procedure can be considered safe, with satisfactory postoperative results.
Liang Zhou, Xingzhe Ji, Lei Wang

2544 related Products with: A Modified Vessel-sparing Microsurgical Vasoepididymostomy.

50 ug 96T4/120 Packing /sleeve/bo4/120 Packing /sleeve/bo100ug Lyophilized100ug Lyophilized0.5 ml1 mg100μl100μg50 ug

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#35753757   2022/06/26 To Up

Ontogeny of TRα1 expression in the mouse testis and epididymis during postnatal development.

Thyroid hormone (T ) acts on the testis via thyroid hormone receptor alpha 1 (TRα1), though the cellular localization of TRα1 in testis remains controversial. Studies on the presence of TRα1 in the epididymis are also lacking. The present study, therefore, examined the cellular localization and expression pattern of TRα1 in testis and epididymis of Parkes mice during postnatal development. Immunohistochemical results showed localization of TRα1 in interstitial and tubular compartments of the testis all through the development. On postnatal day (PND) 14, only leptotene spermatocytes showed TRα1-immunoreactivity in the testis, while at PND 28, 42, and 90, a diverse staining pattern for TRα1 was seen in almost all the seminiferous tubules mainly in leptotene spermatocytes, round and elongating spermatids, and in Leydig cells. Further, qRT-PCR and immunoblot analyses showed that TRα1 was expressed in the testis at the transcript as well as protein level throughout the postnatal development. TRα1 was also seen in principal cells of the epididymis, with maximal expression at PND 90. TRα1 was also present in cauda epididymidal spermatozoa of adult mice at PND 90. The results suggest that TRα1 is expressed in the testis and epididymis and that it may help to regulate the spermatogenic process and male fertility.
Debarshi Sarkar, Asmita Jaiswal, Shio Kumar Singh

1515 related Products with: Ontogeny of TRα1 expression in the mouse testis and epididymis during postnatal development.

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#35753445   2022/06/23 To Up

Perfluorooctanesulfonic acid exposure altered hypothalamic metabolism and disturbed male fecundity.

Previous studies have examined the effects of perfluorooctanesulfonic acid (PFOS) on disruption of the blood-testis barrier and spermatogenesis. Sertoli and Leydig cells were perturbed, resulting in a decrease in testosterone levels and sperm counts. However, the effects of PFOS on male fecundity are not limited to the testes. In this study, we demonstrated that oral PFOS exposure (1 μg/g BW and 5 μg/g BW) decreased the function of the Luteinizing hormone (LH)/Luteinizing hormone receptor (LHr) and decreased epididymal sperm motility. Consistently, testicular transcriptome analysis revealed that PFOS altered the expression of a cluster of genes associated with sperm motility and steroidogenesis. In mice exposed to PFOS, c-Fos immunostaining showed activation of the lateral septal nucleus (LS), paraventricular thalamus (PVT), locus coeruleus (LC), which are known to be related to anxiety-like behaviors. Metabolomic analyses of the hypothalamus revealed that exposure to PFOS perturbed the translation of proteins, as well as the biosynthesis of neurotransmitters and neuromodulators. Altogether, the activation of brain nuclei, shift of hypothalamic metabolome, and reduction of LH/LHr circuit resulted from PFOS exposure suggested the toxicant's systematic effects on male reproduction.
Zijie Li, Ziyi Lin, Shuqin Ji, Keng-Po Lai, Hin-Ting Wan, Chris Kong Chu Wong, Lei Li

1743 related Products with: Perfluorooctanesulfonic acid exposure altered hypothalamic metabolism and disturbed male fecundity.

5 mg1 mg250 MG1 g250 mg10 mg25 mg10 mg 5KG5 mg100 mg100 mg

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#35753026   2022/06/26 To Up

The different effects of intramuscularly-injected lactate on white and brown adipose tissue in vivo.

Lactate is an important product of glycolysis metabolism during exercise and has long been recognized as an important metabolic signaling molecule involved in inhibiting lipolysis and promoting lipogenesis, which consequently leads to regulated adipose tissue metabolism. However, recent studies have shown that lactate promotes the browning of white adipose tissue (WAT), which induces heat production and energy expenditure and ultimately causes weight loss. These studies assessing the effects of lactate on lipid metabolism in adipose tissue have revealed conflicting data, making it an important area worthy of further research.
Yaqian Qu, Siyu Chen, Lei Zhou, Min Chen, Lin Li, Yihan Ni, Jingquan Sun

2645 related Products with: The different effects of intramuscularly-injected lactate on white and brown adipose tissue in vivo.



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#35752031   2022/06/20 To Up

Morphological, morphometric, ultrastructural, and functional evaluation of red-rumped agouti (Dasyprocta leporina) sperm during epididymal transit.

The red-rumped agouti (Dasyprocta leporina) is a hystricognath rodent with reproductive anatomical peculiarities presenting as an intra-abdominal testes-epididymis complex. This study was carried out to describe, for the first time, details related to the morphological and functional changes in sperm along the epididymal transit in agoutis. The testes-epididymal complexes were sampled from seven sexually mature agoutis. Sperm from different epididymal regions (caput, corpus, and cauda) were collected using the floating technique, and their morphology, morphometry, ultrastructure, mitochondrial activity, membrane structural integrity, and kinetic parameters were determined. The number of sperm collected (823.5 ×10 sperm) was higher in the epididymis cauda. No significant differences in normal sperm morphology among the different epididymal regions (caput, 82.42%; corpus, 86.71%; and cauda, 88.86 %) were observed. The mean head length, head width, and tail length were highest in the caput (5.15 µm, 3.44 µm, and 32.04 µm, respectively), decreasing along the epididymal transit. Ultrastructure by scanning electron microscopy (SEM) revealed agglomeration of spermatozoa from caput and corpus, thus, enabling analysis of the gametes from only the epididymal cauda with clarity. Sperm from epididymis cauda showed the greatest proportion of membrane integrity and mitochondrial activity, followed by those from corpus and caput (79.71 %, 58.9 %, 47.7 %, respectively). Significant increase in total motility, progressive motility, velocity average pathway -VAP, velocity straightline - VSL, velocity curvilinear - VCL, and rapid sperm in the caput-corpus-cauda direction were observed. These novel data contribute to the knowledge of sperm maturation in the red-rumped agouti.
Maiko R T Dantas, Andréia M da Silva, Luana G P Bezerra, Ana G Pereira, Nayra R N Luz, João B F Souza-Junior, Moacir F Oliveira, Alexandre R Silva

1239 related Products with: Morphological, morphometric, ultrastructural, and functional evaluation of red-rumped agouti (Dasyprocta leporina) sperm during epididymal transit.

25 mg 5 G10 mg100ul100ug Lyophilized500 MG0.12 mL1 kit(96 Wells)25 mg0.1 mg50 ug

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#35747386   2022/06/07 To Up

Murine double minute 2 aggravates adipose tissue dysfunction through ubiquitin-mediated six-transmembrane epithelial antigen of prostate 4 degradation.

Healthy adipose tissue is crucial to maintain normal energy homeostasis. Little is known about the role of murine double minute 2 (MDM2), an E3 ubiquitin ligase and has been highlighted in oncopathology, in adipose tissue. Our results indicated that MDM2 expression was associated with nutritional status. adipocyte-specific knock-in (-AKI) mice exhibited exacerbated weight gain, insulin resistance, and decreased energy expenditure. Meanwhile, chronic high-fat diet (HFD) exposure caused obvious epididymal white adipose tissue (eWAT) dysfunction, such as senescence, apoptosis, and chronic inflammation, thereby leading to hepatic steatosis in -AKI mice. Mechanically, MDM2 could interact with six-transmembrane epithelial antigen of prostate 4 (STEAP4) and inhibit STEAP4 expression through ubiquitin-mediated STEAP4 degradation. Thereinto, the K18 and K161 sites of STEAP4 were ubiquitin-modificated by MDM2. Finally, STEAP4 restoration in eWAT of -AKI mice on a HFD rescued MDM2-induced adipose dysfunction, insulin resistance, and hepatic steatosis. Summary, the MDM2-STEAP4 axis in eWAT plays an important role in maintaining healthy adipose tissue function and improving hepatic steatosis.
Wei Zhao, Qiang Xu, Jiahui Yang, Xianghong Xie, Chunmei Li, Weihong Zhang, Enhui Chen, Yanfang Guo, Mingyue Gao, Jie Shi, Huabing Zhang, Hong Yao, Meixia Li, Li Yan, Fude Fang, Wenming Wu, Xiaojun Liu

1957 related Products with: Murine double minute 2 aggravates adipose tissue dysfunction through ubiquitin-mediated six-transmembrane epithelial antigen of prostate 4 degradation.

5/200 Packing /sleeve/bo5/200 Packing /sleeve/bo100 ul250ul0.1ml (1mg/ml)

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#35747354   2021/12/15 To Up

Protective effects of Thai (L.) DC. var. pruriens seeds on sexual behaviors and essential reproductive markers in chronic unpredictable mild stress mice.

Chronic stress is a major common cause of male infertility. Many species of velvet beans are shown to be rich in l-DOPA. In Thai folklore medicine, seeds of (L.) DC. var. pruriens (Thai Mhamui or T-MP) have been used for treating erectile dysfunction. This study aimed to determine l-DOPA levels in T-MP seed extract and investigate its preventive on sexual behaviors and reproductive parameter damages including essential proteins in chronic unpredictable mild stress (CUMS) mice.
Pannawat Choowong-In, Jintana Sattayasai, Preecha Boonchoong, Chanasorn Poodendaen, Alexander Th Wu, Nareelak Tangsrisakda, Tarinee Sawatpanich, Supatcharee Arun, Nongnut Uabundit, Sitthichai Iamsaard

2832 related Products with: Protective effects of Thai (L.) DC. var. pruriens seeds on sexual behaviors and essential reproductive markers in chronic unpredictable mild stress mice.

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#35747351   2021/09/25 To Up

The effects of as a libido booster and sexual enhancer on the reproductive system of male rats.

Alongside the advancement of various modern treatments, attention has recently been focused on finding alternative infertility treatments based on diet and herbal medicine. (wild pistachio; Baneh) is a plant species traditionally known as a libido booster and sexual enhancer.
Manijeh Dogani, Nayere Askari, Ali Kalantari-Hesari, Fatemeh Hosseini Rahbar

1462 related Products with: The effects of as a libido booster and sexual enhancer on the reproductive system of male rats.

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