Search results for: Esterase
#34524467 2021/09/15 To Up
Feruloyl esterase Fae1 is required specifically for host colonisation by the rice-blast fungus Magnaporthe oryzae.P
Akhil Thaker, Khyati Mehta, Rajesh Patkar
1141 related Products with: Feruloyl esterase Fae1 is required specifically for host colonisation by the rice-blast fungus Magnaporthe oryzae.250 ml25 µg0.2 mg0.1ml (1mg/ml)1 LITRE1 ml25 µg100 TESTS100 ml0.1 mg0.25 mg0.1 mg
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#34523973 2021/09/15 To Up
Insights into the Lignocellulose-Degrading Enzyme System of var. Based on Genome and Transcriptome Analysis.Humicola grisea var. is a thermophilic ascomycete and important enzyme producer that has an efficient enzymatic system with a broad spectrum of thermostable carbohydrate-active (CAZy) enzymes. These enzymes can be employed in lignocellulose biomass deconstruction and other industrial applications. In this work, the genome of H. grisea var. was sequenced. The acquired sequence reads were assembled into a total length of 28.75 Mbp. Genome features correlate with what was expected for thermophilic Sordariomycetes. The transcriptomic data showed that sugarcane bagasse significantly upregulated genes related to primary metabolism and polysaccharide deconstruction, especially hydrolases, at both pH 5 and pH 8. However, a number of exclusive and shared genes between the pH values were found, especially at pH 8. expresses an average of 211 CAZy enzymes (CAZymes), which are capable of acting in different substrates. The top upregulated genes at both pH values represent CAZyme-encoding genes from different classes, including acetylxylan esterase, endo-1,4-Î²-mannosidase, exoglucanase, and endoglucanase genes. For the first time, the arsenal that the thermophilic fungus var. possesses to degrade the lignocellulosic biomass is shown. Carbon source and pH are of pivotal importance in regulating gene expression in this organism, and alkaline pH is a key regulatory factor for sugarcane bagasse hydrolysis. This work paves the way for the genetic manipulation and robust biotechnological applications of this fungus. Most studies regarding the use of fungi as enzyme producers for biomass deconstruction have focused on mesophile species, whereas the potential of thermophiles has been evaluated less. This study revealed, through genome and transcriptome analyses, the genetic repertoire of the biotechnological relevant thermophile fungus . Comparative genomics helped us to further understand the biology and biotechnological potential of . The results demonstrate that this fungus possesses an arsenal of carbohydrate-active (CAZy) enzymes to degrade the lignocellulosic biomass. Indeed, it expresses more than 200 genes encoding CAZy enzymes when cultivated in sugarcane bagasse. Carbon source and pH are key factors for regulating the gene expression in this organism. This work shows, for the first time, the great potential of as an enzyme producer and a gene donor for biotechnological applications and provides the base for the genetic manipulation and robust biotechnological applications of this fungus.
Andrei Stecca Steindorff, Luana Assis Serra, Eduardo Fernandes Formighieri, FabrÃcia Paula de Faria, Marcio JosÃ© PoÃ§as-Fonseca, JoÃ£o Ricardo Moreira de Almeida
1046 related Products with: Insights into the Lignocellulose-Degrading Enzyme System of var. Based on Genome and Transcriptome Analysis.100 U500 Units1 system1100 ug 70 Slides 25 ml Ready-to-use
#34517642 2021/08/05 To Up
A near-infrared light triggered fluormetric biosensor for sensitive detection of acetylcholinesterase activity based on NaErF: 0.5Â % [email protected] upconversion nano-probe.Acetylcholinesterase (AChE), as an important neurotransmitter, is widely present in the peripheral and central nervous systems. The aberrant expression of AChE could cause diverse neurodegenerative diseases. Herein, we developed a facile and interference-free fluorimetric biosensing platform for highly sensitive AChE activity determination based on a NaErF: 0.5Â % [email protected] nano-probe. This nano-probe exhibits a unique property of emitting bright monochromic red (650Â nm) upconversion (UC) emission under multiband (~808, ~980, and ~1530Â nm) near-infrared (NIR) excitations. The principle of this detection relies on the quenching of the strong monochromic red UC emission by oxidization products of 3,3',5,5'-tetramethylbenzidine generated through AChE-modulated cascade reactions. This system shows a great sensing performance with a detection limit (LOD) of 0.0019 mU mL for AChE, as well as good specificity and stability. Furthermore, we validated the potential of the nano-probe in biological samples by determination of AChE in whole blood with a LOD of 0.0027 mU mL, indicating the potential application of our proposed platform for monitoring the progression of AChE-related disease.
Xu Zhao, Ling Zhang, Xu Yan, Li Zhang, Yang Lu, Jialin Pan, Meiling Zhang, Chenguang Wang, Hui Suo, Xiaoteng Jia, Xiaomin Liu, Geyu Lu
2069 related Products with: A near-infrared light triggered fluormetric biosensor for sensitive detection of acetylcholinesterase activity based on NaErF: 0.5Â % [email protected] upconversion nano-probe.100tests100tests100tests192 rxns2x96 well plates
#34517595 2021/07/21 To Up
DNase I-assisted 2'-O-methyl molecular beacon for amplified detection of tumor exosomal microRNA-21.An end-modified 2'-O-methyl molecular beacon (eMB) with unique nuclease resistance was designed and prepared. The eMB can resist the enzymatic digestion by DNase I, which would otherwise occur upon the hybridization of the eMB with a complementary sequence. As a result, the coupling use of eMBs and DNase I allows highly sensitive detection of miRNA with a limit of detection (LOD) of 2.5 pM. The analytical strategy was further used for detection of tumor exosomal microRNA-21, and down to 0.86Â Î¼gÂ mL A375 exosomes were detected. Overall, the present method can effectively quantify tumor-derived exosomes for cancer diagnosis.
Haiyan Zheng, Qingyuan Lin, Jinchao Zhu, Yamin Rao, Liang Cui, Yongyang Bao, Tianhai Ji
2554 related Products with: DNase I-assisted 2'-O-methyl molecular beacon for amplified detection of tumor exosomal microRNA-21.1L100 mg1 g1 mg1 mg5 mg200 mg1 mg20 ug1 mg 2x5L
#34515720 2021/09/13 To Up
A feasible self-assembled near-infrared fluorescence sensor for acid phosphatase detection and cell imaging.The single signal amplification strategy is significant for detecting various disease biomarkers but is restricted by its limited accuracy. The multi-signal and multi-mode methods have overcome this deficiency. Acid phosphatase (ACP) is an important intracellular enzyme but one-step cell imaging material-based probes are scarce for ACP. Herein, we designed a one-step self-assembled polymer probe using neutral red (NR), modified-(pyridoxal-5'-phosphate (PLP)) and Eu. The polymer exhibited non-emission and excellent stability. Upon the catalytic hydrolysis reaction of ACP, the polymer exhibited two strong fluorescence signals at 373 nm and 613 nm and an appreciable decline of absorbance at 395 nm. The probe has excellent selectivity and higher sensitivity with a limit of detection as low as 0.02 mU mL. It possesses favorable biocompatibility and has been successfully used to detect and image intracellular ACP in several living cells.
Shuangqin Li, Yaya Wang, Shuai Mu, Jinlong Zhang, Xiaoyan Liu, Syed Faheem Askari Rizvi, Haixia Zhang, Nana Ding, Lan Wu
2072 related Products with: A feasible self-assembled near-infrared fluorescence sensor for acid phosphatase detection and cell imaging.1 kit1 kit1 kit1 kit1 kit 1 G1 kit 1 G1 kit
#34515710 2021/09/13 To Up
Photoelectrochemical analysis of the alkaline phosphatase activity in single living cells.Conventional photoelectrochemical (PEC) analysis mostly utilizes photoactive material modified planar indium tin oxides (ITOs) to obtain photocurrent responses for the measurement of analytes in solution. In this work, a CdS quantum dot (QD) modified nanopipette was prepared for the PEC analysis of the alkaline phosphatase (ALP) activity in single MCF-7 cells. The nanopipette was filled with ascorbic acid 2-phosphate (AAP) that was egressed outside the nanopipette by electrochemical pumping. Next, AAP was catalyzed by ALP to generate ascorbic acid (AA), which is an efficient electron donor for CdS QDs under illumination. Based on the result that the nanopipette showed a linear photocurrent response to AA, a nearly linear correlation between the photocurrent and the activity of ALP was established. Accordingly, using these CdS QD modified nanopipettes, the ALP activity in single MCF-7 cells was determined to be 0.12 U mL by PEC analysis. This work does not expand the application of PEC bioanalysis, but offers a new strategy for single cell analysis.
Nina Wang, Rongrong Pan, Lina Ji, Dechen Jiang, Hong-Yuan Chen
2784 related Products with: Photoelectrochemical analysis of the alkaline phosphatase activity in single living cells.900 tests500 assays100ul100ul1 kit 100ul100ul1 mg
#34511539 2021/09/13 To Up
Acute myelomonocytic leukemia negative for alpha-naphthyl acetate esterase stain in a Holstein cow.A 4-year, 7-month-old Holstein cow presented with anorexia. Physical examination revealed masses in the interscapular region and vagina. Blast cells were detected in the masses and peripheral blood by fine needle aspiration cytology and hematological examination. By bone marrow aspiration, blast cells constituted up to 24.2% of all nucleated cells, and 22% and 2% of non-erythroid cells stained positive for myeloperoxidase and alpha-naphthyl acetate esterase (ANAE), respectively. Pathological examination revealed the mass lesions consisted of a proliferation of tumor cells, which were positive for monocytic markers (HLA-DR and Iba-1). The cow was diagnosed with acute myelomonocytic leukemia (AMML). Even when tumor cells are ANAE-negative, AMML cannot be completely ruled out and should be considered when diagnosing cattle with leukemia/lymphoma.
Masaki Maezawa, Ai Nakamichi, Nao Akiyama, Michihito Tagawa, Ken-Ichi Watanabe, Yoshiyasu Kobayashi, Hisashi Inokuma
1717 related Products with: Acute myelomonocytic leukemia negative for alpha-naphthyl acetate esterase stain in a Holstein cow.0.2 mL 1 G100ug2 100ug Lyophilized100ug Lyophilized100μg100ug100ug Lyophilized100ug Lyophilized
#34509837 2021/09/02 To Up
Developmental toxicity assessment of 4-MBC in Danio rerio embryo-larval stages.Enormous production of cosmetic products and its indiscriminate use tends to discharge into the aquatic environment and might threaten non-target organisms inhabiting aquatic ecosystems. In the present study, developmental toxicity of 4-methylbenzylidene camphor (4-MBC), a widely used organic UV filter in personal care products has been evaluated using zebrafish embryo-larval stages. Waterborne exposure induced developmental toxicity and deduced 2.71Â mg/L as 96Â h LC whereas embryos exposed to sub-lethal concentrations (50 and 500Â Î¼g/L) caused a significant delay in hatching rate, heart rate, reduced larval length, and restricted hatchlings motility besides the axial curvature. Chronic exposure to 10 dpf resulted in significant decrease in SOD activity at 500Â Î¼g/L with no changes in CAT level besides a significant increase in GST enzyme at 5Â Î¼g/L concentration in 5 dpf sampled larvae. However, all the three enzymes were significantly elevated in 10 dpf larvae indicating differential oxidative stress during the stages of development. Similar trend is noticed for acetylcholine esterase enzyme activity. A concentration dependent increase in malondialdehyde content was noted in larvae sampled at 5 and 10 dpf. In addition, multixenobiotic resistance (MXR) activity inhibition, and elevated oxidative tissue damage were noticed at 5 dpf with no significant changes in 10 dpf larvae. Furthermore, immunoblot analysis confirms 4-MBC induced apoptosis in zebrafish larvae with promoted cleaved Caspase-3, Bax and inhibited Bcl-2 proteins expression. Subsequently, docking studies revealed the binding potential of 4-MBC to zebrafish Abcb4 and CYP450 8A1 proteins with the binding energy of -8.1 and -8.5Â kcal/mol representing target proteins interaction and toxicity potentiation. Our results showed that 4-MBC exposure triggers oxidative stress at sub-lethal concentrations leading to apoptosis, deformities and locomotion perturbations in developing zebrafish.This is first of its kind in systematically demonstrating developmental toxicity of 4-MBC and the information shall be used for aquatic toxicity risk assessment.
Ved Prakash, Veena Jain, Shweta Singh Chauhan, Ramakrishnan Parthasarathi, Somendu K Roy, Sadasivam Anbumani
2920 related Products with: Developmental toxicity assessment of 4-MBC in Danio rerio embryo-larval stages.100μg100 μg-100ug100 μg0.1 mg2 1 Set
#34508340 // To Up
Bioefficacy of mosquito mat vaporizers and associated metabolic detoxication mechanisms in Aedes aegypti (Linnaeus) in Selangor, Malaysia: A statewide assessment.This study aims to examine the efficacy of mosquito mat vaporizers on Aedes aegypti and their associated metabolic detoxication mechanisms. For this purpose, Aedes aegypti (Linnaeus) was collected from nine districts in Selangor, Malaysia and tested with mosquito vaporizing mat bioassays. The same populations were also subjected to biochemical assays to investigate activities of detoxifying enzymes, namely non-specific esterase (EST), glutathione-S-transferase (GST) and mixed function oxidase (MFO). The efficacy of Ae. aegypti on the active ingredients tested in decreasing order were d- allethrin > dimefluthrin > prallethrin with PBO > prallethrin. The results further indicated significant enhancement mean levels of EST, GST and MFO in pyrethroid-resistant populations. The mortality rate of Ae. aegypti in response to pyrethroid active ingredients was associated with MFO activity, suggesting it is an important detoxification enzyme for the populations tested. In view of the presence of resistance against household insecticide products, pyrethroid efficacy on Ae. aegypti populations needs to be monitored closely to ensure the implementation of an effective vector control program in Malaysia.
T Azratul-Hizayu, C D Chen, K W Lau, N Azrizal-Wahid, T K Tan, Y A L Lim, M Sofian-Azirun, V L Low
2228 related Products with: Bioefficacy of mosquito mat vaporizers and associated metabolic detoxication mechanisms in Aedes aegypti (Linnaeus) in Selangor, Malaysia: A statewide assessment.5mg1 ml
#34506858 2021/09/07 To Up
Mining of a novel esterase (est3S) gene from a cow rumen metagenomic library with organosphosphorus insecticides degrading capability: Catalytic insights by site directed mutations, docking, and molecular dynamic simulations.A novel esterase (est3S) gene, 1026Â bp in size, was cloned from a metagenomic library made of uncultured microorganisms from the contents of cow rumen. The esterolytic enzyme (Est3S) is composed of 342 amino acids and shows the highest identity with EstGK1 (71.7%) and EstZ3 (63.78%) esterases from the uncultured bacterium. The Est3S did not cluster in any up-to-date classes (I to XVIII) of esterase and lipase. Est3S protein molecular weight was determined to be 38Â kDa by gel electrophoresis and showed optimum activity at pHÂ 7.0 and 40Â Â°C and is partially resistant to organic solvents. Est3S activity was enhanced by K, Na, Mg, and Ca and its highest activity was observed toward the short-chain p-nitrophenyl esters. Additionally, Est3S can degrade chlorpyrifos (CP) and methyl parathion (70% to 80%) in an hour. A mutated Est3S (Ser132-Ala132) did not show any activity toward CP and ester substrates. Notably, the GHS132QG motif is superimposed with the homolog esterase and cutinase-like esterase. Therefore, Ser132 is the critical amino acid like other esterases. The Est3S is relatively stable with ester compounds, and the methyl parathion complex was confirmed by molecular dynamics simulation. NOVELTY STATEMENT: A novel esterase gene (est3S) expressing esters and organophosphorus insecticide degradation traits was isolated from the uncultured bacterium in the contents of cow rumen. The Est3S protein did not cluster in any up-to-date classes (I to XVIII) of esterase/lipase proteins. Est3S was stable with the ligands up to 100Â ns during the molecular dynamic simulations.
Hee Yul Lee, Du Yong Cho, Iqrar Ahmad, Harun M Patel, Min Ju Kim, Jea Gack Jung, Eun Hye Jeong, Md Azizul Haque, Kye Man Cho
1433 related Products with: Mining of a novel esterase (est3S) gene from a cow rumen metagenomic library with organosphosphorus insecticides degrading capability: Catalytic insights by site directed mutations, docking, and molecular dynamic simulations.100μg50 ug100ug50 ug10 mg50 ug48 samples50 ug50 ug
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