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#32790727   2020/08/13 To Up

Excreta biomarkers in response to different gut barrier dysfunction models and probiotic supplementation in broiler chickens.

Increased intestinal permeability (IP) and inflammation are both linked with functionality of the intestinal barrier and in particular enterocytes. Currently, almost all assessment methods of the intestinal barrier function are invasive. The present study aimed to quantify selected proteins as novel biomarkers in excreta of broiler chickens to facilitate non-invasive assessment of gut barrier function using enzyme-linked immunosorbent assays (ELISA). It was further hypothesised that probiotics as feed additives may counteract gut barrier dysfunction. A 3 × 2 factorial arrangement of treatments was used with the main factors being gut barrier dysfunction models (control, rye-based diet, and dexamethasone-DEX) with and without probiotic supplementation (a three-strain Bacillus) using 72 male Ross 308 day-old chickens. Each of the 6 experimental treatments was replicated 12 times. On d 21 of age, fluorescein isothiocyanate dextran (FITC-d) uptake into serum was examined to test IP. Fresh excreta samples were collected on d 20. The biomarkers included alpha-1 antitrypsin (A1AT), intestinal fatty acid binding protein (I-FABP), lipocalin-2 (LCN2), fibronectin (FN), intestinal alkaline phosphatase (IAP), ovotransferrin (OVT) and superoxide dismutase [Cu-Zn] (SOD1). Only DEX increased (P<0.001) FITC-d passage to the blood on d 21 of age, indicating a greater IP. The excreta concentrations of A1AT, I-FABP and SOD1 were unaltered by the experimental treatments. DEX increased (P<0.05) FN concentration in excreta compared with control birds. Conversely, inclusion of rye in the diet reduced (P<0.05) FN but increased (P<0.001) OVT in excreta. Independently, DEX decreased IAP (P<0.05) in excreta compared with control and rye-fed birds. The excreta concentration of LCN2 tended (P = 0.086) to increase in birds injected by DEX. There was no demonstrable effect of probiotic addition on any of the studied parameters. Among the tested biomarkers, FN, IAP, and LCN2 revealed promise as biomarkers of intestinal barrier function quantified by ELISA kits.
Reza Barekatain, Gordon S Howarth, Nicky-Lee Willson, David Cadogan, Stuart Wilkinson

2315 related Products with: Excreta biomarkers in response to different gut barrier dysfunction models and probiotic supplementation in broiler chickens.

1 kit500 gm. 1 G1mg100 μg100 1 kit100 mg5025

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#32790695   2020/08/13 To Up

Identification of peptides interfering with the LRRK2/PP1 interaction.

Serine/threonine phosphatases are responsible for modulating the activities of the protein kinases implicated in the development of several pathologies. Here we identified by a PEP-scan approach a peptide of LRRK2, a Parkinson's disease associated protein, interacting with the phosphatase PP1. In order to study its biological activity, the peptide was fused via its N-terminal to an optimized cell penetrating peptide. We synthesized from the original peptide five interfering peptides and identified two (Mut3DPT-LRRK2-Short and Mut3DPT-LRRK2-Long) able to disrupt the LRRK2/PP1 interaction by competition in anti-LRRK2 immunoprecipitates. Using FITC-labelled peptides, we confirmed their internalization into cell lines as well as into primary cells obtained from healthy or ill human donors. We confirmed by ELISA test the association of Mut3DPT-LRRK2-Long peptide to purified PP1 protein. The peptides Mut3DPT-LRRK2-5 to 8 with either N or C-terminal deletions were not able to disrupt the association LRRK2/PP1 nor to associate with purified PP1 protein. The interfering sequences blocking the PP1/LRRK2 interaction were also fused to a shuttle peptide able to cross the blood brain barrier and showed that the newly generated peptides BBB-LRRK2-Short and BBB-LRRK2-Long were highly resistant to protease degradation. Furthermore, they blocked PP1/LRRK2 interaction and they penetrated into cells. Hence, these newly generated peptides can be employed as new tools in the investigation of the role of the LRRK2/PP1 interaction in normal and pathological conditions.
Chang Zhi Dong, Heriberto Bruzzoni-Giovanelli, Yanhua Yu, Karim Dorgham, Christophe Parizot, Jean Marc Zini, Jean Yves Brossas, Pierre Tuffery, Angelita Rebollo

2678 related Products with: Identification of peptides interfering with the LRRK2/PP1 interaction.

1 Set1 Set1 Set1 Set1 Set1 Set1 Set1 Set1 Set96T1 Set

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#32787821   2020/08/12 To Up

Effect of sub-chronic exposure to cigarette smoke, electronic cigarette and waterpipe on human lung epithelial barrier function.

Taking into consideration a recent surge of a lung injury condition associated with electronic cigarette use, we devised an in vitro model of sub-chronic exposure of human bronchial epithelial cells (HBECs) in air-liquid interface, to determine deterioration of epithelial cell barrier from sub-chronic exposure to cigarette smoke (CS), e-cigarette aerosol (EC), and tobacco waterpipe exposures (TW).
Baishakhi Ghosh, Hermes Reyes-Caballero, Sevcan Gül Akgün-Ölmez, Kristine Nishida, Lakshmana Chandrala, Lena Smirnova, Shyam Biswal, Venkataramana K Sidhaye

1903 related Products with: Effect of sub-chronic exposure to cigarette smoke, electronic cigarette and waterpipe on human lung epithelial barrier function.

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#32787053   2020/08/03 To Up

Bimetallic Thin Film Combination of Surface Plasmon Resonance based Optical Fiber Cladding with the Polarizing Homodyne Balanced Detection Method and Biomedical Assay Application.

In this work, we present the optical birefringence properties of the optical fiber cladding that exist as an evanescent field where the refractive index (RI) of the analysis solution is applied for optical sensor aspiration. To enhance the performance of the sensor, we have investigated with different thickness of TiO coating and bimetallic (Ag-Al) film alloy combinations by thermal evaporation coating. We described a special balanced homodyne detection method for the intensity difference change between the and polarization lights in the surface plasmon resonance (SPR) sensing systems is strongly determined by the refractive index of the test medium. The plasmonic optical fiber can measure a very small change of the RI of a glycerol solution, which is a resolution of 4.37×10 refractive index unit (RIU). This method has great potential of a small-sized optical setup, high stability, high selectivity, easily chemically modified and low cost. Furthermore, due to the experiment results, our approach can also eliminate the surrounding noise in Mach-Zehnder interferometer which showing the feasibility of this proposed technique. We demonstrate the fluorescence enhancement in detecting C-Reactive protein (CPR) antibody conjugated with fluorescein isothiocyanate (FITC) by means of near-field coupling between surface plasmons and fluorophores at spectral channels of emission. This technique can also be extended for application in a biomedical assay and biochemical science, including molecular diagnostics relying on multi-channels that require a small volume of analyte at each channel which would suffer from weakness of fluorescence if it were not for the enhancement technology.
Vu Thi Huong, Vinh Van Tran, Nae Yoon Lee, Dung Van Hoang, Kieu The Loan Trinh, Thang Bach Phan, Nhu Hoa Thi Tran

2697 related Products with: Bimetallic Thin Film Combination of Surface Plasmon Resonance based Optical Fiber Cladding with the Polarizing Homodyne Balanced Detection Method and Biomedical Assay Application.

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#32786858   2020/08/04 To Up

Pharmacokinetics and Transport of an Osteogenic Dodecapeptide.

A dodecapeptide with the amino acid sequence of IEELEEELEAER (PIE), identified from Mytilus edulis proteolysis hydrolysates, has shown good bone-forming activity in previous studies. The pharmacokinetics and transport of PIE peptide in vivo or in vitro were investigated in this study. Results showed that PIE peptide can be transported into monolayer Caco-2 cells, PIE peptide was identified in the serum after the mice reached the highest value of 173.60 ± 60.30 ng/mL, in which it was quantified by an optimized mass spectrometry method. In addition, PIE peptide has a promoting effect on the BMP pathway at the gene and protein levels. By observing the distribution of orally administrated PIE-FITC in ovariectomized mice, it was confirmed that it can enter the gastrointestinal tract, serum and reached the bone. Taken together, PIE peptide can be absorbed well both in vitro and in vivo, and it could promote pre-osteoblast differentiation factors.
Zhe Xu, Hui Chen, Fengjiao Fan, Pujie Shi, Shuzhen Cheng, Maolin Tu, Hesham El-Seedi, Ming Du

1267 related Products with: Pharmacokinetics and Transport of an Osteogenic Dodecapeptide.

5 G0.1 mg25 mg0.1ml (1mg/ml)2.5 mg 5 G100ug Lyophilized100ug100ug200ul1 g100ul

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#32786505   2020/08/11 To Up

Design, Synthesis, Conjugation, and Reactivity of Novel -1,5-Cyclooctadiene-Derived Bioorthogonal Linkers.

The tetrazine/-cyclooctene (TCO) inverse electron-demand Diels-Alder (IEDDA) reaction is the fastest bioorthogonal "click" ligation process reported to date. In this context, TCO reagents have found widespread applications; however, their availability and structural diversity is still somewhat limited due to challenges connected with their synthesis and structural modification. To address this issue, we developed a novel strategy for the conjugation of TCO derivatives to a biomolecule, which allows for the creation of greater structural diversity from a single precursor molecule, i.e., -1,5-cyclooctadiene [(,)-COD] , whose preparation requires standard laboratory equipment and readily available reagents. This two-step strategy relies on the use of new bifunctional TCO linkers (-) for IEDDA reactions, which can be synthesized via 1,3-dipolar cycloaddition of (,)-COD with different azido spacers (-) carrying an electrophilic function (NHS-ester, -succinimidyl carbonate, -nitrophenyl-carbonate, maleimide) in the ω-position. Following bioconjugation of these electrophilic linkers to the nucleophilic residue (cysteine or lysine) of a protein (step 1), the resulting TCO-decorated constructs can be subjected to a IEDDA reaction with tetrazines functionalized with fluorescent or near-infrared (NIR) tags (step 2). We successfully used this strategy to label bovine serum albumin with the TCO linker and subsequently reacted it in a cell lysate with the fluorescein-isothiocyanate (FITC)-derived tetrazine . The same strategy was then used to label the bacterial wall of Gram-positive , showing the potential of these linkers for live-cell imaging. Finally, we determined the impact of structural differences of the linkers upon the stability of the bioorthogonal constructs. The compounds for stability studies were prepared by conjugation of TCO linkers , , and to mAbs, such as Rituximab and Obinutuzumab, and subsequent labeling with a reactive Cy3-functionalized tetrazine.
Beatrice Longo, Chiara Zanato, Monica Piras, Sergio Dall'Angelo, Albert D Windhorst, Danielle J Vugts, Massimiliano Baldassarre, Matteo Zanda

1991 related Products with: Design, Synthesis, Conjugation, and Reactivity of Novel -1,5-Cyclooctadiene-Derived Bioorthogonal Linkers.

100ug100ug Lyophilized100ug Lyophilized100ul2ug100ug Lyophilized100ug Lyophilized10 nmol100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug

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#32785841   2020/08/13 To Up

Evaluation of Blood-Brain Barrier Integrity Using Vascular Permeability Markers: Evans Blue, Sodium Fluorescein, Albumin-Alexa Fluor Conjugates, and Horseradish Peroxidase.

The blood-brain barrier (BBB) constituted by endothelial cells of brain microvessels is a dynamic interface, which controls and regulates the transport of various substances including peptides, proteins, ions, vitamins, hormones, and immune cells from the circulation into the brain parenchyma. Certain diseases/disorders such as Alzheimer's disease, sepsis, and hypertension can lead to varying degrees of BBB disruption. Moreover, impairment of BBB integrity has been implicated in the pathogenesis of various neurodegenerative diseases like epilepsy. In attempts to explore the wide spectrum of pathophysiologic mechanisms of these diseases/disorders, a variety of experimental insults targeted to the BBB integrity in vitro in cell culture models and in vivo in laboratory animals have been shown to alter BBB permeability causing enhanced transport of certain tracers such as sodium fluorescein, cadaverine-Alexa fluor, horseradish peroxidase, FITC-dextran, albumin-Alexa fluor conjugates, and Evans blue dye across the barrier. The permeability changes in barrier-type endothelial cells can be assessed by intravascular infusion of exogenous tracers and subsequent detection of the extravasated tracer in the brain tissue, which enable functional and structural analysis of BBB integrity. In this chapter, we aimed to highlight the current knowledge on the use of four most commonly performed tracers, namely, Evans blue, sodium fluorescein, albumin-Alexa fluor conjugates, and horseradish peroxidase. The experimental methodologies that we use in our laboratory for the detection of these tracers by macroscopy, spectrophotometry, spectrophotofluorometry, confocal laser scanning microscopy, and electron microscopy are also discussed. Tracing studies at the morphological level are mainly aimed at the identification of the tracers both in the barrier-related cells and brain parenchyma. In addition, BBB permeability to the tracers can be quantified using spectrophotometric and spectrophotofluorometric assays and image analysis by confocal laser scanning microscopy and electron microscopy. The results of our studies conducted under various experimental settings using the mentioned tracers indicate that barrier-type endothelial cells in brain microvessels orchestrate the paracellular and/or transcellular trafficking of substances across BBB. These efforts may not only contribute to designing approaches for the management of diseases/disorders associated with BBB breakdown but may also provide new insights for developing novel brain drug delivery strategies.
Bulent Ahishali, Mehmet Kaya

1209 related Products with: Evaluation of Blood-Brain Barrier Integrity Using Vascular Permeability Markers: Evans Blue, Sodium Fluorescein, Albumin-Alexa Fluor Conjugates, and Horseradish Peroxidase.

100μg10 g100μg100μg100ug100μg100μg100ug100μg100μg100ug Lyophilized100ug Lyophilized

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#32783744   2020/08/12 To Up

New triterpenoid saponin from the stems of (Schumach.) W.Wight.

As part of our continuing study of apoptosis-inducing saponins from Cameroonian genus, one new triterpenoid saponin, named adianthifolioside J (), together with the known gummiferaoside E (), were isolated from stems. The structure of the new saponin (), was established on the basis of extensive analysis of 1 D and 2 D NMR (H-, C-NMR, DEPT, COSY, TOCSY, NOESY, HSQC, HSQC-TOCSY and HMBC) and HRESIMS experiments, and by chemical evidence as 3--[-D-xylopyranosyl-(1→2)--D-fucopyranosyl-(1→6)--D-glucopyranosyl]-21--{(2E,6S)-2-(hydroxymethyl)-6-methyl-6--{4--[(2E,6S)-2,6-dimethyl-6--(-D-quinovopyranosyl)octa-2,7-dienoyl]-(-D-quinovopyranosyl)octa-2,7-dienoyl]}acacic acid-28---D-glucopyranosyl-(1→3)-[5--acetyl--L-arabinofuranosyl-(1→4)]--L-rhamnopyranosyl-(1→2)--D-glucopyranosyl ester ( The pro-apoptotic activity of the new isolated saponin was evaluated, using Annexin V-FITC binding assay, on the A431 human epidermoid cancer cell. The result showed that adianthifolioside J () displayed weak pro-apoptotic activity.
Daniel Djou Toukea, Eutrophe Le Doux Kamto, Line Made Simo, Joséphine Ngo Mbing, Cyril Antheaume, Mohamed Haddad, Olivier Placide Noté, Dieudonné Emmanuel Pegnyemb

1406 related Products with: New triterpenoid saponin from the stems of (Schumach.) W.Wight.

25500 Units(50mg)11mg12000 IU100.00 ul

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#32782452   2020/08/08 To Up

Effects of HPV16 E6 protein on Daxx-induced apoptosis in C33A cells.

Daxx is a highly conserved nuclear protein with an important role in transcription, apoptosis and other cell processes. We investigated the role of HPV16 E6 in Daxx-induced apoptosis through their interactions in C33A cells.
Shuangyang Tang, Shuang Ding, Lan Yu, Haiyan Shen, Yanping Wan, Yimou Wu

2965 related Products with: Effects of HPV16 E6 protein on Daxx-induced apoptosis in C33A cells.

1 Set1 Set2 Pieces/Box96T1 Set1 Set1 Set1 Set1 Set1 Set1mg

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#32782437   2020/08/07 To Up

Tanshinone IIA regulates human AML cell proliferation, cell cycle, and apoptosis through miR-497-5p/AKT3 axis.

The roots of are used in traditional Chinese medicine (TCM) and have high medicinal value. Tanshinone IIA (Tan IIA) is the active ingredient of which can inhibit the growth of acute leukemia cell lines in vitro, although the mechanism remains unclear.
Zi-Yuan Nie, Ming-Hui Zhao, Bao-Qian Cheng, Rong-Fang Pan, Tian-Rui Wang, Yan Qin, Xue-Jun Zhang

2245 related Products with: Tanshinone IIA regulates human AML cell proliferation, cell cycle, and apoptosis through miR-497-5p/AKT3 axis.

0.5 mg5 x 50 ug 100ul50 ug0.5 mg500 assays1.00 flask

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