Search results for: Fluor
#31799532 // Save this To Up
Folding of single-stranded circular DNA into rigid rectangular DNA accelerates its cellular uptake.Despite the importance of the interaction between DNA and cells for its biological activity, little is known about exactly how DNA interacts with cells. To elucidate the relationship between the structural properties of DNA and its cellular uptake, a single-stranded circular DNA of 1801 bases was designed and folded into a series of rectangular DNA (RecDNA) nanostructures with different rigidities using DNA origami technology. Interactions between these structures and cells were evaluated using mouse macrophage-like RAW264.7 cells. RecDNA with 50 staple DNAs, including four that were Alexa Fluor 488-labeled, was designed. RecDNA with fewer staples, down to four staples (all Alexa Fluor 488-labeled), was also prepared. Electrophoresis and atomic force microscopy showed that all DNA nanostructures were successfully obtained with a sufficiently high yield. Flow cytometry analysis showed that folding of the single-stranded circular DNA into RecDNA significantly increased its cellular uptake. In addition, there was a positive correlation between uptake and the number of staples. These results indicate that highly folded DNA nanostructures interact more efficiently with RAW264.7 cells than loosely folded structures do. Based on these results, it was concluded that the interaction of DNA with cells can be controlled by folding using DNA origami technology.
2300 related Products with: Folding of single-stranded circular DNA into rigid rectangular DNA accelerates its cellular uptake.Human Dnak (HSP70) His ta Goat Anti-Human MDG1 DNAJ Rabbit Anti-ERdj5 DNAJC10 E. coli SSB (Single Stran Rabbit Anti-ERdj5 DNAJC10 Protease, DNASE free hea Recombinant E. coli HSP70 Recombinant Human DNase P Recombinant E. coli HSP70 Rabbit Anti-DNase gamma P Rabbit Anti-DNase gamma P Rabbit Anti-DNase gamma P
#31793753 // Save this To Up
Influence of carbon source complexity on porosity, water retention and extracellular matrix composition of Neurospora discreta biofilms.To evaluate carbon source complexity as a process lever to impact the microstructure, chemical composition and water retention capacity of biofilms produced by Neurospora discreta.
2393 related Products with: Influence of carbon source complexity on porosity, water retention and extracellular matrix composition of Neurospora discreta biofilms.Ofloxacin CAS Number [824 Carbonic anhydrase 9 anti RAP2C, member of RAS onco Tropomyosin 2 antibody So NDUFS4 antibody Source Ra COX4 antibody Source Rabb Human VEGF-165, Unlabeled PEX6 antibody Source Rabb Epoxide hydrolase 1 antib 20S Proteasome alpha 6 an Anti-AMPA Receptor 2 (Glu SP1 (Phospho-Thr739) Anti
#31778475 // Save this To Up
Influence of the mode of application of universal adhesive systems on adhesive properties to fluorotic enamel.The objective of this study is to compare the resin-enamel bond strength (mμSBS), in-situ degree of conversion (DC), and the enamel-etching pattern (SEM/EDX) of universal adhesive systems when applied to sound and fluorotic enamel. Ninety-eight human molars were sectioned into 4 parts and divided into 24 groups according to 1) enamel surface (sound or fluorotic enamel), 2) adhesive system (Clearfil Universal Bond [CUB], Futurabond U [FBU], iBond Universal [IBU], and Scotchbond Universal [SBU]), and 3) application mode (etch-and-rinse [ER], active self-etch [Active-SE], and passive self-etch [Passive-SE]). Specimens were stored at 37 °C, for 24 hours and tested at 1.0 mm/min (μSBS). Enamel-resin interfaces were evaluated for in-situ DC. The enamel-etching pattern was evaluated under a SEM/EDX. Data from mμSBS and in-situ DC was analyzed using a three-way ANOVA and Tukey's test at 5 % level of significance. For all adhesives, the ER resulted in a statistically significant higher mean mμSBS than the passive-SE in both substrates (p < 0.001). For all adhesives, active-SE resulted in mean mμSBS (p > 0.31) and in-situ DC (p > 0.45) that were statistically similar to those obtained with the ERs in both substrates. A statistically significant, higher mean mμSBS and in-situ DC were obtained in sound enamel (p < 0.001) than in fluorotic enamel. In general, SBU showed higher mean values for mμSBS and in-situ DC compared to those of CUB and IBU (p < 0.001). ER and active-SE showed the deepest enamel-etching pattern in both substrates. A higher amount of fluor was observed in fluorotic enamel. The active application of universal adhesives in the SE-mode may be a viable alternative to increase the adhesive properties in sound and fluorotic enamel.
1785 related Products with: Influence of the mode of application of universal adhesive systems on adhesive properties to fluorotic enamel.Ofloxacin CAS Number [824 FDA Standard Frozen Tissu TCP-1 theta antibody Sour FDA Standard Frozen Tissu TOM1L1 antibody Source Ra Rabbit Anti-Clostridium b RAP2C, member of RAS onco TOM1-like protein 2 antib FDA Standard Frozen Tissu FDA Standard Frozen Tissu Rabbit Anti-Fascin Actin Mouse Anti-HDAC2 Target A
#31763978 // Save this To Up
Apolipoprotein M-bound sphingosine-1-phosphate regulates blood-brain barrier paracellular permeability and transcytosis.The blood-brain barrier (BBB) is formed by the endothelial cells lining cerebral microvessels, but how blood-borne signaling molecules influence permeability is incompletely understood. We here examined how the apolipoprotein M (apoM)-bound sphingosine 1-phosphate (S1P) signaling pathway affects the BBB in different categories of cerebral microvessels using ApoM deficient mice (). We used two-photon microscopy to monitor BBB permeability of sodium fluorescein (376 Da), Alexa Fluor (643 Da), and fluorescent albumin (45 kDA). We show that BBB permeability to small molecules increases in mice. Vesicle-mediated transfer of albumin in arterioles increased 3 to 10-fold in mice, whereas transcytosis in capillaries and venules remained unchanged. The S1P receptor 1 agonist SEW2871 rapidly normalized paracellular BBB permeability in mice, and inhibited transcytosis in penetrating arterioles, but not in pial arterioles. Thus, apoM-bound S1P maintains low paracellular BBB permeability in all cerebral microvessels and low levels of vesicle-mediated transport in penetrating arterioles.
2246 related Products with: Apolipoprotein M-bound sphingosine-1-phosphate regulates blood-brain barrier paracellular permeability and transcytosis.erythro-ω-Amino Sphingos Rabbit Whole Blood 100ml Apolipoprotein A I (APO A Chicken Whole Blood 50ml Apolipoprotein B (APO B) anti H inh human blood an Rhesus Monkey Whole Blood Bovine Androstenedione,AS Rabbit Anti-Human Androge CD-1 Mouse Whole Blood K3 Brain tumor and normal ti Rat monoclonal anti mouse
#31741725 // Save this To Up
Single molecule characterization of individual extracellular vesicles from pancreatic cancer.Biofluid-accessible extracellular vesicles (EVs) may represent a new means to improve the sensitivity and specificity of detecting disease. However, current methods to isolate EVs encounter challenges when they are used to select specific populations. Moreover, it has been difficult to comprehensively characterize heterogeneous EV populations at the single vesicle level. Here, we robustly assessed heterogeneous EV populations from cultured cell lines via nanoparticle tracking analysis, proteomics, transcriptomics, transmission electron microscopy, and quantitative single molecule localization microscopy (qSMLM). Using qSMLM, we quantified the size and biomarker content of individual EVs. We applied qSMLM to patient plasma samples and identified a pancreatic cancer-enriched EV population. Our goal is to advance single molecule characterization of EVs for early disease detection. : EV: Extracellular Vesicle; qSMLM: quantitative Single Molecule Localization Microscopy; PDAC: Pancreatic Ductal Adenocarcinoma; EGFR: epidermal growth factor receptor 1; CA19-9: carbohydrate antigen 19-9; SEC: size exclusion chromatography; WGA: wheat germ agglutinin; AF647: Alexa Fluor 647; Ab: antibody; HPDEC: Healthy Pancreatic Ductal Epithelial Cell; TEM: Transmission Electron Microscopy.
1645 related Products with: Single molecule characterization of individual extracellular vesicles from pancreatic cancer.Mid advanced stage pancre Multiple pancreatic cance Pancreatic cancer tissue Multiple pancreatic cance High density pancreatic c Pancreatic cancer tissue Multiple pancreatic cance Multiple pancreatic cance Pancreatic cancer and nor Pancreatic cancer tissue Pancreatic disease spectr Multiple pancreatic cance
#31713439 // Save this To Up
Multiple retrograde tracing methods compatible with 3DISCO clearing.Exploring the spatial relationship of various neuron pools in the spinal cord is crucial and difficult due to its complexity. The single-labelling tracing and sectioning were employed in previous studies exploring the distribution of spinal motor neuron pools, which could only delineate one single motor neuron pool in one specimen and could not achieve intact-tissue observation. Here, with combination of neuroanatomy tracing techniques and the optical clearing technique, we developed a multiple retrograde tracing method compatible with 3DISCO clearing. Fluoro-Gold, Fluoro-Ruby, Cholera Toxin Subunit B, Alexa Fluor 488 and 647 Conjugate were injected intramuscularly in hindlimbs of C57BL/6 adults. After labelling, the harvested spinal cords were optically cleared by 3DISCO method and imaged using confocal microscope. There were positive signals of all four tracers and four motor neurons pools targeting injected muscles were labelled. Three-dimension model of four motor neuron pools was successfully reconstructed based on tomography images showing the spatial relationship of different neuron pools. In conclusion, using this method, we first delineated the spatial relationship of four different motor neuron pools targeting four skeletal muscles in one spinal cord at the same time, which provide a holistic view of motor neuron pools in the spinal cord.
Multiple breast cancer ti FDA standard normal rat m Multiple cancer tissue ar Multiple myeloma test tis In vitro Human Retina Mic Normal rat multiple organ Multiple carcinoma tissue Chemokine (Human) Quantit AEE-788 Mechanisms: Multi Multiple uterine carcinom Multiple brain cancer tis Multiple malignant melano
#31705210 // Save this To Up
A dual-signal colorimetric and ratiometric fluorescent nanoprobe for enzymatic determination of uric acid by using silicon nanoparticles.The authors describe a dual-signal colorimetric and ratiometric fluorescent probe for uric acid (UA). It is based on cascade catalysis and an inner filter effect. The method involves uricase-catalyzed oxidation of UA and iodide-catalyzed oxidation of the colorless peroxidase substrate o-phenylenediamine (OPD) to form yellow 2,3-diaminophenazine (oxOPD). This can be visually observed or monitored by measuring absorbance at 417 nm. Furthermore, oxOPD quenches the fluorescence of silicon nanoparticles (SiNPs) (with peaks at 450 and 565 nm) via an inner filter effect. The change in the ratio of emissions peaking 565 and 450 (at excitation wavelength of 380 nm) increases linearly in the 0.01-0.8 mM UA concentration range). The lower detection limits are 8.4 and 0.75 μM when using the colorimetric and ratiometric fluorometric method, respectively. The assay was successfully applied to the quantitation of UA in spiked serum samples. Graphical abstractA dual-signal colorimetric and ratiometric fluor ometric assay was developed for uric acid (UA). The fluorometric assay is based on the inner filter effect between fluorescent silicon nanoparticles and 2,3-diaminophenazine. It involves uricase-catalyzed oxidation of UA, and iodide-catalyzed oxidation of o-phenylenediamine.
1522 related Products with: A dual-signal colorimetric and ratiometric fluorescent nanoprobe for enzymatic determination of uric acid by using silicon nanoparticles.EnzyChrom™ Free Fatty A EnzyChrom™ D-Lactate As EnzyChrom™ Ascorbic Aci EnzyChrom™ D-Lactate As 5 (2 Aminoethylamino) 1 n QuantiChrom™ Formaldehy 2 Fluoro 5 formylphenylbo Androst-4-ene-3,17-dion-1 5(6) Carboxyfluorescein h EnzyChrom™ Acetylcholin 5 Formyl 2 thiopheneboron Uric Acid, Uricase Color
#31702581 // Save this To Up
Inexpensive robotic system for standard and fluorescent imaging of protein crystals.Protein-crystallization imaging and classification is a labor-intensive process typically performed either by humans or by instruments that currently cost well over $100 000. This cost puts the use of crystallization-trial imaging outside the reach of most academic laboratories, and also start-up biotechnology firms, where resources are scarce. An imaging system has been designed and prototyped which automatically captures images from multi-well protein-crystallization experiments using both standard and fluorescent imaging techniques at a cost 28 times lower than current market rates. The machine uses a Panowin F1 3D printer as a base and controls it using G-code commands sent from a Python script running on a desktop computer. A graphical user interface (GUI) was developed to enable users to control the machine and facilitate image capture, classification and editing. A 488 nm laser diode and a 525 nm filter were incorporated to allow in situ fluorescent imaging of proteins trace-labeled with a fluorophore, Alexa Fluor 488. The instrument was primarily designed using a 3D printer and augmented using commercially available parts, and this publication aims to serve as a guide for comparable in-laboratory robotics projects.
2365 related Products with: Inexpensive robotic system for standard and fluorescent imaging of protein crystals.Rabbit Anti-Rat Androgen 5 (2 Aminoethylamino) 1 n Slider Imager with LED Tr Cyan Fluorescent Protein PsaC | protein standard Yellow Fluorescent Protei Anti-BMP-1 (Bone Morphoge Protein A (Liquid form) 2',7' Dichlorofluorescein PsbA | D1 protein standar dsRed Fluorescent Protein MarkerGeneTM Fluorescent
#31675755 // Save this To Up
Ferritin Light Chain (FTL) competes with long noncoding RNA Linc00467 for miR-133b binding site to regulate chemoresistance and metastasis of colorectal cancer.Although the colorectal cancer (CRC) mortality rates are decreasing in virtue of CRC screening and improved therapeutic methods, CRC is still a leading cause of cancer deaths. One of the main causes is chemoresistance occurrence in CRC. Understanding of the molecular mechanisms of chemoresistance benefits to CRC diagnosis and treatment. In this study, gene expression was determined by Western blot and qRT-PCR. The biological functions of genes in CRC cells were studied by knocking down or overexpressing the gene in CRC cells and then analyzing cell sensitivity to 5-Fu by the MTT assay and the flow cytometry, and analyzing cell migration and invasion by transwell assays. The luciferase reporter assay was used to examine microRNA regulation of target gene expression, and biotin pulldown assay was performed to detect interaction between RNA molecules. This study found that Ferritin Light Chain (FTL) and long intergenic non-coding RNA Linc00467 were both upregulated in CRC tissues and cell lines, and inversely correlated to CRC patient survival. FTL and Linc00467 promoted CRC cells abilities to resistance against 5-fluor-ouracil (5-Fu), migration and invasion. These effects were compromised by miR-133b which targeted both FTL and Linc00467. miR-133b interacted with Linc00467 and miR-133b inhibitor prevented Linc00467 knockdown-induced alternations of FTL expression and biological functions. Both FTL and Linc00467 are oncogenes in CRC. FTL expression upregulated in CRC via Linc00467/ miR-133b axis, and leads to CRC cell resistance against 5-FU treatment and promotes CRC metastasis.
2548 related Products with: Ferritin Light Chain (FTL) competes with long noncoding RNA Linc00467 for miR-133b binding site to regulate chemoresistance and metastasis of colorectal cancer.Colorectal (colon and rec Colorectal (colon and rec anti Ferritin Heavy chain Goat Anti-Human Lambda Li Rabbit Anti-IgG, heavy an Anti- ADAM-12 (A Disintig Rabbit Anti-Human Kappa L Mouse Anti-Lambda Light C Mid advanced stage ovary Kappa Light Chain Recombinant Human Ferriti Oral cavity (tongue and p
#31659562 // Save this To Up
Clinical-grade N-(4-[F]fluorobenzoyl)-interleukin-2 for PET imaging of activated T-cells in humans.Molecular imaging of immune cells might be a potential tool for response prediction, treatment evaluation and patient selection in inflammatory diseases as well as oncology. Targeting interleukin-2 (IL2) receptors on activated T-cells using positron emission tomography (PET) with N-(4-[F]fluorobenzoyl)-interleukin-2 ([F]FB-IL2) could be such a strategy. This paper describes the challenging translation of the partly manual labeling of [F]FB-IL2 for preclinical studies into an automated procedure following Good Manufacturing Practices (GMP), resulting in a radiopharmaceutical suitable for clinical use.
2377 related Products with: Clinical-grade N-(4-[F]fluorobenzoyl)-interleukin-2 for PET imaging of activated T-cells in humans.Rectum cancer test tissue Lung cancer survey tissue Cervix cancer tissue arra Head and neck cancer tiss Cervix cancer survey tiss Multiple head and neck ca Breast cancer and normal Endometrial cancer test t Lung cancer tissue array, Multiple organ tumor with Bladder cancer tissue arr Breast cancer tissue arra
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45 Fax 0032 16 50 90 45
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50 Fax 01 43 25 01 60
52062 Aachen Deutschland
Tel 0241 40 08 90 86 Fax 0241 55 91 05 36
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
Schweiz Züri +41435006251
Česká republika Praha +420246019719
Ireland Dublin +35316526556
Norge Oslo +4721031366
Finland Helsset +358942419041
Sverige Stockholm +46852503438
Ελλάς Αθήνα +302111768494
Magyarország Budapest +3619980547
GENTAUR Poland Sp. z o.o.
ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
Tel 058 710 33 44
Fax 058 710 33 48
GENTAUR Nederland BV
5521 DG Eersel Nederland
Tel 0208-080893 Fax 0497-517897
Piazza Giacomo Matteotti, 6, 24122 Bergamo
Tel 02 36 00 65 93 Fax 02 36 00 65 94
53 Iskar Str. 1191 Kokalyane, Sofia