Search results for: Fluor
#33079440 2020/10/20 To Up
A general strategy to design highly fluorogenic far-red and near-infrared tetrazine bioorthogonal probes.Highly fluorogenic tetrazine bioorthogonal probes emitting at near-infrared wavelengths are in strong demand for biomedical imaging applications. Herein, we have developed a strategy for forming a palette of novel "Huaxi-Fluor" probes in situ , whose fluorescence increases hundreds of times upon forming of the bioorthogonal reaction product, pyridazine. The resulting probes show large Stokes shifts and high quantum yields. Manipulating the conjugate length and pull-push strength in the fluorophore skeleton allows the emission wavelength to be fine-tuned from 556 to 728 nm. The highly photo-stable and biocompatible probes are suitable for visualizing organelles in live cells without a washing step and for imaging of tumors in live small animals to depths of 500 μm by two-photon excitation.
Wuyu Mao, Jie Tang, Liqun Dai, Xinyu He, Jie Li, Larry Cai, Ping Liao, Ruotian Jiang, Jingwei Zhou, Haoxing Wu
2155 related Products with: A general strategy to design highly fluorogenic far-red and near-infrared tetrazine bioorthogonal probes.0.1ml (1.3mg/ml)1 module4 Arrays/Slide1 kit(96 Wells)1000 tests 100ul2 Pieces/Box1 module1 mg100 assays1 module
#33071313 2020/10/10 To Up
7-Imidazolyl-substituted 4'-methoxy and 3',4'-dimethoxy-containing polyfluoroflavones as promising antiviral agents.A simple and convenient method for the synthesis of new methyl 2-(4-methoxyphenyl)- and 2-(3,4-dimethoxyphenyl)-4-oxo-4-polyfluorochromen-3-carboxylates as analogs of natural methoxy-containing flavones is proposed. As a result of their directed modification under basic conditions, 7-imidazolyl-substituted derivatives were obtained. In aqueous-organic medium under basic conditions, 5,6,7,8-tetrafluoro-3-(methoxycarbonyl)flavones were transformed into 6,8-difluoro-5-hydroxy-7-(1-imidazol-1-yl)-3-(methoxycarbonyl)flavones as a result of rearrangement, while 6,7,8-trifluorinated analogs underwent a rearrangement to give 6,8-difluoro-3-(hydroxyarylidene)-7-(1-imidazol-1-yl)coumarins under the same conditions. Acid hydrolysis of methyl polyfluoroflavone-3-carboxylates allowed to obtain 2-aryl-4-polyfluorochromen-4-ones. Evaluation of the antiviral activity of the synthesized compounds against influenza A (H1N1) and Coxsackie B3 viruses showed that 2-(3,4-dimethoxyphenyl)-5,6,8-trifluoro-7-(1-imidazol-1-yl)-4-chromene-4-one has the most promising properties.
Konstantin V Shcherbakov, Mariya A Artemyeva, Yanina V Burgart, Victor I Saloutin, Alexandrina S Volobueva, Maria A Misiurina, Yana L Esaulkova, Ekaterina O Sinegubova, Vladimir V Zarubaev
2044 related Products with: 7-Imidazolyl-substituted 4'-methoxy and 3',4'-dimethoxy-containing polyfluoroflavones as promising antiviral agents.1 G100μl100ul7 x 25 assays100 plates100 tests100 assays100 assays200 assays25 g
#33033365 2020/10/08 To Up
Restricted intramolecular rotation of fluorescent molecular rotors at the periphery of aqueous microdroplets in oil.F
Jooyoun Kang, SangMoon Lhee, Jae Kyoo Lee, Richard N Zare, Hong Gil Nam
2232 related Products with: Restricted intramolecular rotation of fluorescent molecular rotors at the periphery of aqueous microdroplets in oil.10reactions 100 20 µl (10 mM)100 μg10reactions 4 Membranes/Box100 μg10reactions
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#33020470 2020/10/05 To Up
Unscrambling fluorophore blinking for comprehensive cluster detection via photoactivated localization microscopy.Determining nanoscale protein distribution via Photoactivated Localization Microscopy (PALM) mandates precise knowledge of the applied fluorophore's blinking properties to counteract overcounting artifacts that distort the resulting biomolecular distributions. Here, we present a readily applicable methodology to determine, optimize and quantitatively account for the blinking behavior of any PALM-compatible fluorophore. Using a custom-designed platform, we reveal complex blinking of two photoswitchable fluorescence proteins (PS-CFP2 and mEOS3.2) and two photoactivatable organic fluorophores (PA Janelia Fluor 549 and Abberior CAGE 635) with blinking cycles on time scales of several seconds. Incorporating such detailed information in our simulation-based analysis package allows for robust evaluation of molecular clustering based on individually recorded single molecule localization maps.
René Platzer, Benedikt K Rossboth, Magdalena C Schneider, Eva Sevcsik, Florian Baumgart, Hannes Stockinger, Gerhard J Schütz, Johannes B Huppa, Mario Brameshuber
2385 related Products with: Unscrambling fluorophore blinking for comprehensive cluster detection via photoactivated localization microscopy.2x96 well plate4x96 well plate 100 G100tests100 assays96 wells0.1 mg1 x 10^6 cells/vial25 assays
#33015016 2020/09/09 To Up
To What Extent Do Fluorophores Bias the Biological Activity of Peptides? A Practical Approach Using Membrane-Active Peptides as Models.The characterization of biologically active peptides relies heavily on the study of their efficacy, toxicity, mechanism of action, cellular uptake, or intracellular location, using both and studies. These studies frequently depend on the use of fluorescence-based techniques. Since most peptides are not intrinsically fluorescent, they are conjugated to a fluorophore. The conjugation may interfere with peptide properties, thus biasing the results. The selection of the most suitable fluorophore is highly relevant. Here, a comprehensive study with blood-brain barrier (BBB) peptide shuttles (PepH3 and PepNeg) and antimicrobial peptides (AMPs) (vCPP2319 and Ctn[15-34]), tested as anticancer peptides (ACPs), having different fluorophores, namely 5(6)-carboxyfluorescein (CF), rhodamine B (RhB), quasar 570 (Q570), or tide fluor 3 (TF3) attached is presented. The goal is the evaluation of the impact of the selected fluorophores on peptide performance, applying routinely used techniques to assess cytotoxicity/toxicity, secondary structure, BBB translocation, and cellular internalization. Our results show that some fluorophores significantly modulate peptide activity when compared with unlabeled peptides, being more noticeable in hydrophobic and charged fluorophores. This study highlights the need for a careful experimental design for fluorescently labeled molecules, such as peptides.
Marco Cavaco, Clara Pérez-Peinado, Javier Valle, Rúben D M Silva, João D G Correia, David Andreu, Miguel A R B Castanho, Vera Neves
1854 related Products with: To What Extent Do Fluorophores Bias the Biological Activity of Peptides? A Practical Approach Using Membrane-Active Peptides as Models.1 kit1 kit100 Tests1 kit(96 Wells) 96 Tests 100 assays10 ug
#33014604 2020/08/19 To Up
Intravital longitudinal imaging of hepatic lipid droplet accumulation in a murine model for nonalcoholic fatty liver disease.Nonalcoholic fatty liver disease (NAFLD) is a rapidly increasing chronic liver disorder worldwide accompanied by hepatic steatosis, inflammation, fibrosis, and severe liver failure. Unfortunately, an effective treatment strategy for NAFLD has not yet been established, which has been hampered by the limited understanding of the pathophysiological drivers for NAFLD. To examine the unknown cellular and molecular mechanisms in the pathogenesis of NAFLD, there is an increasing need for the direct observation of hepatic microenvironments over extended periods of time. In this work, using a custom-built intravital imaging system and a novel fluorescent lipid droplet labeling dye, Seoul-Fluor 44 (SF44), we established an intravital imaging method to visualize individual lipid droplets and microvasculature simultaneously in the liver of live mice . In addition, in the nonalcoholic steatosis and steatohepatitis mouse model induced by a methionine and choline-deficient diet, we longitudinally visualized and quantitatively analyzed the development of lipid droplets in hepatocytes and sinusoid at a subcellular resolution during the progression of NAFLD up to 21 days .
Jieun Moon, Eunji Kong, Jingu Lee, Jinjoo Jung, Eunha Kim, Seung Bum Park, Pilhan Kim
1684 related Products with: Intravital longitudinal imaging of hepatic lipid droplet accumulation in a murine model for nonalcoholic fatty liver disease.
#33013401 2020/09/08 To Up
Neurochemistry of Enteric Neurons Following Prolonged Indomethacin Administration in the Porcine Duodenum.G
Marta Czajkowska, Jarosław Całka
1291 related Products with: Neurochemistry of Enteric Neurons Following Prolonged Indomethacin Administration in the Porcine Duodenum.50 mg2 250 mg25 mg 25 G100 10 100
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#32996731 // To Up
Osteopontin siRNA does not confer resistance to toxic effects of parthenolide in Jurkat cells.Osteopontin (OPN) plays a critical role in cell proliferation and drug resistance in cancer treatment and hematological malignancies. In T cell acute lymphoblastic leukemia, most initial therapies can induce remission while some patients then relapse and do not respond well to chemotherapy. The sesquiterpene lactone parthenolide (PTL) can induce apoptosis in a variety of cancer cell lines via inhibition of pro-inflammatory transcription factor nuclear factor kappa B and has anti-tumor activity in acute lymphoblastic leukemia treatment.
S Mehri, S Mohammadi, M Nikbakht, M Sahmani, M Zahedpanah
1035 related Products with: Osteopontin siRNA does not confer resistance to toxic effects of parthenolide in Jurkat cells.500 gm.96 tests1 mg1 mg100 extractions25 100 μg2.50 nmol1 mg1x10e7 cells
#32996289 2020/09/29 To Up
Correlations between available primary amines, endospore coat thickness, and alkaline glutaraldehyde sensitivity for spores of select Bacillus species.Alkaline glutaraldehyde (GTA) is a high-level chemical disinfectant/sterilant and has a broad microbial kill spectrum. The precise antimicrobial mechanism of GTA remains debated. GTA kill times are extremely variable across different organisms, illustrating the need for a better understanding of GTA kill mechanisms related to different organisms. A commonly proposed GTA kill mechanism suggests that it works by cross-linking accessible primary amines on important surface proteins. If true, the antimicrobial activity of GTA may directly correlate to the number of these available functional groups. Bacillus species form highly resistant bacterial endospores that are commonly used as one of the most stringent test organisms for disinfection and sterilization. In this study, we compared the log reduction times of alkaline GTA on spores from 4 Bacillus species to fluorescent profiles generated using Alexa Fluor™ amine-reactive dyes. GTA kill times were also compared to mean spore coat thicknesses as measured with scanning electron microscopy (SEM). Fluorescence values generated from bound amine-reactive dye showed a strong, positive correlation to GTA susceptibility, as measured by GTA 6-log reduction times. Spore coat thickness also showed a strong, positive correlation to reduction time values. Results support the hypothesis that GTA kill times are directly related to the number of available primary amines on bacterial endospores. Results also indicated that the killing efficacy of GTA may be influenced by its ability to penetrate the spore coat to reach additional targets, suggesting that damaging important biomolecules beyond surface proteins may be involved in GTA killing mechanisms.
Jacob Kent Player, Justen Thalmus Despain, Richard A Robison
1383 related Products with: Correlations between available primary amines, endospore coat thickness, and alkaline glutaraldehyde sensitivity for spores of select Bacillus species.100ug100ug250 ml100ug 100ul100ug0.2 mg100ug 100ul100ug50 ug 100μl
#32939382 2020/08/27 To Up
Biophysical characterization dataset of native nicotinic acetylcholine receptor in lipid-like detergent complexes.For a long time, traditional purification and extraction methods for the native nicotinic acetylcholine receptor in lipid-like detergent complex (nAChR-DC) have compromised its purity, functionality and X-ray structural studies possibility. The dataset presented in this article provide a characterization of the nAChR-DC purified using a sequential purification processes developed in our laboratory . This purification takes in consideration all of the physicochemical and functional requirements stablished by several researchers for the past three decades for the nAChR. These requirements were addressed in order to preserve the stability and functionality of nAChR-DC while ensuring the highest degree of protein purity. We focused on the effect of cholesteryl hemisuccinate (CHS) supplementation on nAChR conformational changes during the purification process. Data from the size exclusion chromatography of the nAChR-DC supplemented with CHS in concentrations ranging from 0.01 mM, 0.1 mM, 0.2 mM and 0.5 mM consistently demonstrated that 0.5 mM CHS affects receptor stability via disassemble of the pentameric oligomer. However, 0.2 mM CHS produced negligible nAChR-DC subunit disruption. The purified nAChR-DC has been characterized by circular dichroism (CD) and fluorescence recovery after photobleaching (FRAP) in order to assess its stability. The CD data was recorded in the wavelength range of 190250 nm, showed that CHS induce a ⍺-helix to β-sheet transition of the nAChR-DC. The nAChR-LFC-16 delipidation with Methyl-β-Cyclodextrin decreased the percentage of α-helix and increased the β-sheet antiparallel secondary structure and levels the percentage of turns to that of the nAChR-DC without CHS treatment. Additionally, the stability of the nAChR-DC supplemented with CHS and incorporated into lipid cubic phase (LCP) was monitored for a period of 30 days by means of FRAP. The LCP-FRAP data allowed to establish possible optimal crystallization conditions for the development of crystals from purified nAChR-conjugated to α-Bungarotoxin, Alexa Fluor ™ 488 (α-BTX) in order to obtain a high-resolution atomic structure by X-ray diffraction.
Rafael Maldonado-Hernández, Orestes Quesada, José A Lasalde-Dominicci
1501 related Products with: Biophysical characterization dataset of native nicotinic acetylcholine receptor in lipid-like detergent complexes.5050 96T100ug Lyophilized10100ug100ug1mg10 100ug Lyophilized10 100 μg
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