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#35030259   2022/01/14 To Up

Dose-dependent Transmissibility of Chromosome Aberrations at First Mitosis after Exposure to Gamma Rays. I. Modeling and Implications Related to Risk Assessment.

The relationship between certain chromosomal aberration (CA) types and cell lethality is well established. On that basis we used multi-fluor in situ hybridization (mFISH) to tally the number of mitotic human lymphocytes exposed to graded doses of gamma rays that carried either lethal or nonlethal CA types. Despite the fact that a number of nonlethal complex exchanges were observed, the cells containing them were seldom deemed viable, due to coincident lethal chromosome damage. We considered two model variants for describing the dose responses. The first assumes independent linear-quadratic (LQ) dose response shapes for the yields of both lethal and nonlethal CAs. The second (simplified) variant assumes that the mean number of nonlethal CAs per cell is proportional to the mean number of lethal CAs per cell, meaning that the shapes and magnitudes of both aberration types differ only by a multiplicative proportionality constant. Using these models allowed us to assemble dose response curves for the frequency of aberration-bearing cells that would be expected to survive. This took the form of a joint probability distribution for cells containing ≥1 nonlethal CAs but having zero lethal CAs. The simplified second model variant turned out to be marginally better supported than the first, and the joint probability distribution based on this model yielded a crescent-shaped dose response reminiscent of those observed for mutagenesis and transformation for cells "at risk" (i.e. not corrected for survival). Among the implications of these findings is the suggestion that similarly shaped curves form the basis for deriving metrics associated with radiation risk models.
Bradford D Loucas, Igor Shuryak, Stephen R Kunkel, Michael N Cornforth

1586 related Products with: Dose-dependent Transmissibility of Chromosome Aberrations at First Mitosis after Exposure to Gamma Rays. I. Modeling and Implications Related to Risk Assessment.

50 µg1 module0.1 mg100 μg100 assays25 g 5 G1 module1 mgOne 96-Well Strip Micropl30 m Pcs Per Pack

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#35027775   2021/05/30 To Up

A Selective Single Step Amidation of Polyfluoroarenes.

This chemistry establishes a method for the synthesis of per- and poly-fluoroaryl acid amides, utilizing nucleophilic aromatic substitution. Traditionally, such amides are constructed in a two-step process, namely, ammonolysis and then -acylation. Herein, good yields of polyfluoroaryl acid amides were achieved in a single step under mild reaction conditions. Key to achieving optimal yields is the use of two equivalents of the nucleophile. In addition, the mechanism of the reaction is discussed which has implications for other related nucleophilic substitutions.
Alyssa M Noel, Matthew Hamilton, Brockton Keen, Megan Despain, Jon Day, Jimmie D Weaver

1174 related Products with: A Selective Single Step Amidation of Polyfluoroarenes.

1ml1mg 500 gm 1ml1ml1mg5 mg1ml10 0.1mg1 Set

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#34990957   2021/12/25 To Up

Plasmonically-enhanced competitive assay for ultrasensitive and multiplexed detection of small molecules.

Novel methods that enable facile, ultrasensitive and multiplexed detection of low molecular weight organic compounds such as metabolites, drugs, additives, and organic pollutants are valuable in biomedical research, clinical diagnosis, food safety and environmental monitoring. Here, we demonstrate a simple, rapid, and ultrasensitive method for detection and quantification of small molecules by implementing a competitive immunoassay with an ultrabright fluorescent nanolabel, plasmonic fluor. Plasmonic-fluor is comprised of a polymer-coated gold nanorod and bovine serum albumin conjugated with molecular fluorophores and biotin. The synthesis steps and fluorescence emission of plasmonic-fluor was characterized by UV-vis spectroscopy, transmission electron microscopy, and fluorescence microscopy. Plasmon-enhanced competitive assay can be completed within 20 min and exhibited more than 30-fold lower limit-of-detection for cortisol compared to conventional competitive ELISA. The plasmon-enhanced competitive immunoassay when implemented as partition-free digital assay enabled further improvement in sensitivity. Further, spatially multiplexed plasmon-enhanced competitive assay enabled the simultaneous detection of two analytes (cortisol and fluorescein). This simple, rapid, and ultrasensitive method can be broadly employed for multiplexed detection of various small molecules in research, in-field and clinical settings.
Zheyu Wang, Qingjun Zhou, Anushree Seth, Samhitha Kolla, Jingyi Luan, Qisheng Jiang, Priya Rathi, Prashant Gupta, Jeremiah J Morrissey, Rajesh R Naik, Srikanth Singamaneni

2831 related Products with: Plasmonically-enhanced competitive assay for ultrasensitive and multiplexed detection of small molecules.

50 assays400 assays2x96 well plate100tests500 tests100 assays100tests100 assays25 assays100tests

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#34960580   2021/12/20 To Up

Estimation of Fluor Emission Spectrum through Digital Photo Image Analysis with a Water-Based Liquid Scintillator.

In this paper, we performed a feasibility study of using a water-based liquid scintillator (WbLS) for conducting imaging analysis with a digital camera. The liquid scintillator (LS) dissolves a scintillating fluor in an organic base solvent to emit light. We synthesized a liquid scintillator using water as a solvent. In a WbLS, a suitable surfactant is needed to mix water and oil together. As an application of the WbLS, we introduced a digital photo image analysis in color space. A demosaicing process to reconstruct and decode color is briefly described. We were able to estimate the emission spectrum of the fluor dissolved in the WbLS by analyzing the pixel information stored in the digital image. This technique provides the potential to estimate fluor components in the visible region without using an expensive spectrophotometer. In addition, sinogram analysis was performed with Radon transformation to reconstruct transverse images with longitudinal photo images of the WbLS sample.
Ji-Won Choi, Ji-Young Choi, Kyung-Kwang Joo

1355 related Products with: Estimation of Fluor Emission Spectrum through Digital Photo Image Analysis with a Water-Based Liquid Scintillator.

15 ml 120 ml 1 mg 30 ml 1000 ml 500 ml

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#34958937   2021/12/24 To Up

Involvement of autophagy in the maintenance of rat intervertebral disc homeostasis: an in-vitro and in-vivo RNA interference study of Atg5.

In the largest avascular low-nutrient intervertebral disc, resident cells would utilize autophagy, a stress-response survival mechanism by self-digestion and recycling wastes. Our goal was to elucidate the involvement of autophagy in disc homeostasis through RNA interference of autophagy-related gene 5 (Atg5).
R Tsujimoto, T Yurube, Y Takeoka, Y Kanda, K Miyazaki, H Ohnishi, Y Kakiuchi, S Miyazaki, Z Zhang, T Takada, R Kuroda, K Kakutani

1121 related Products with: Involvement of autophagy in the maintenance of rat intervertebral disc homeostasis: an in-vitro and in-vivo RNA interference study of Atg5.

10 mg200ul10 mg100 μg25 mg100 μg

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#34947657   2021/12/06 To Up

Polarization Angle Dependence of Optical Gain in a Hybrid Structure of Alexa-Flour 488/M13 Bacteriophage.

We measured optical modal gain of a dye-virus hybrid structure using a variable stripe length method, where Alexa-fluor-488 dye was coated on a virus assembly of M13 bacteriophage. Inspired by the structural periodicity of the wrinkle-like virus assembly, the edge emission of amplified spontaneous emission was measured for increasing excited optical stripe length, which was aligned to be either parallel or perpendicular to the wrinkle alignment. We found that the edge emission showed a strong optical anisotropy, and a spectral etalon also appeared in the gain spectrum. These results can be attributed to the corrugated structure, which causes a similar effect to a DFB laser, and we also estimated effective cavity lengths.
Inhong Kim, Juyeong Jang, Seunghwan Lee, Won-Geun Kim, Jin-Woo Oh, Irène Wang, Jean-Claude Vial, Kwangseuk Kyhm

1999 related Products with: Polarization Angle Dependence of Optical Gain in a Hybrid Structure of Alexa-Flour 488/M13 Bacteriophage.

100ug Lyophilized20 ul100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized50 ul100ug Lyophilized100ug Lyophilized100ug Lyophilized

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#34944664   2021/12/06 To Up

Gold(I) Complexes Bearing Alkylated 1,3,5-Triaza-7-phosphaadamantane Ligands as Thermoresponsive Anticancer Agents in Human Colon Cells.

Overheating can affect solubility or lipophilicity, among other properties, of some anticancer drugs. These temperature-dependent changes can improve efficiency and selectivity of the drugs, since they may affect their bioavailability, diffusion through cell membrane or activity. One recent approach to create thermosensitive molecules is the incorporation of fluorine atoms in the chemical structure, since fluor can tune some chemical properties such as binding affinity. Herein we report the anticancer effect of gold derivatives with phosphanes derived from 1,3,5-triaza-7-phosphaadamantane (PTA) with long hydrocarbon chains and the homologous fluorinated chains. Besides, we analysed the influence of temperature in the cytotoxic effect. The studied gold(I) complexes with phosphanes derived from PTA showed antiproliferative effect on human colon carcinoma cells (Caco-2/TC7 cell line), probably by inhibiting cellular TrxR causing a dysfunction in the intracellular redox state. In addition, the cell cycle was altered by the activation of p53, and the complexes produce apoptosis through mitochondrial depolarization and the consequent activation of caspase-3. Furthermore, the results suggest that this cytotoxic effect is enhanced by hyperthermia and the presence of polyfluorinated chains.
Javier Quero, Francesco Ruighi, Jesús Osada, M Concepción Gimeno, Elena Cerrada, Maria Jesús Rodriguez-Yoldi

1535 related Products with: Gold(I) Complexes Bearing Alkylated 1,3,5-Triaza-7-phosphaadamantane Ligands as Thermoresponsive Anticancer Agents in Human Colon Cells.

10 ug1 mg1.00 flask100 μg1.00 flask100 μg1.00 flask96 assays0.5 ml1.00 flask100 μg

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#34944412   2021/11/25 To Up

Cholecystokinin-B Receptor-Targeted Nanoparticle for Imaging and Detection of Precancerous Lesions in the Pancreas.

Survival from pancreatic cancer remains extremely poor, in part because this malignancy is not diagnosed in the early stages, and precancerous pancreatic intraepithelial neoplasia (PanIN) lesions are not seen on routine radiographic imaging. Since the cholecystokinin-B receptor (CCK-BR) becomes over-expressed in PanIN lesions, it may serve as a target for early detection. We developed a biodegradable fluorescent polyplex nanoparticle (NP) that selectively targets the CCK-BR. The NP was complexed to a fluorescent oligonucleotide with Alexa Fluor 647 for far-red imaging and to an oligonucleotide conjugated to Alexa Fluor 488 for localization by immunohistochemistry. Fluorescence was detected over the pancreas of five- to ten-month-old LSL-Kras; (KC) mice only after the injection of the receptor target-specific NP and not after injection of untargeted NP. Ex vivo tissue imaging and selective immunohistochemistry confirmed particle localization only to PanIN lesions in the pancreas and not in other organs, supporting the tissue specificity. A human pancreas tissue microarray demonstrated immunoreactivity for the CCK-BR only in the PanIN lesions and not in normal pancreas tissue. The long-term goal would be to develop this imaging tool for screening human subjects at high risk for pancreatic cancer to enable early cancer detection.
Jill P Smith, Hong Cao, Elijah F Edmondson, Siva Sai Krishna Dasa, Stephan T Stern

1203 related Products with: Cholecystokinin-B Receptor-Targeted Nanoparticle for Imaging and Detection of Precancerous Lesions in the Pancreas.

100ug100ug100ug250 mg100ug Lyophilized100ug96 wells (1 kit)100ug100ug Lyophilized

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