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Search results for: GSTA1

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#39444352   2024/10/24 To Up

Molecular and Cellular Characterization of the Glutathione Transferases Involved in the Olfactory Metabolism of the Mammary Pheromone.

Odorant metabolizing enzymes, considered as critical olfactory perireceptor actors, control the odor molecules reaching the olfactory epithelium by biotransforming them. As an odorant, the mammary pheromone, i.e., 2-methylbut-2-enal (2MB2), emitted in the milk of lactating female rabbits triggers typical nipple searching-grasping behavior through orocephalic movements in newborn rabbits but not in weaned rabbits. We previously showed that 2MB2 perception is significantly modified when its glutathione transferase-dependent olfactory metabolism is affected in newborns. Here, enzymatic assays of the recombinant enzymes GSTA1, M1, and P1 revealed the activity of these enzymes toward the mammary pheromone. Histological experiments revealed strong expression of the GSTA class restricted to the Bowman glands and of GSTP1 in the nuclei of sustentacular cells. Moreover, some modulations of GSTs have been demonstrated, including a significant increase in GSTP1 expression (2-fold in mRNA, value < 0.001; protein, value: 0.031) after 45 min of mammary pheromone exposure at 10 g/mL and an increase in GSTA expression in weaned rabbits compared with newborn rabbits (3-fold in mRNA, value: 0.011; protein, value: 0.001). Our results provide new insights into the activity, cellular expression, and modulation of the mammary pheromone GST-metabolizing enzymes and clues about their olfactory function.
Marie-Sabelle Hjeij, Franck Ménétrier, Isabelle Chauvel, Nicolas Poirier, Stéphane Fraichard, Gwenaëlle Steyaert, Quentin Bonnin, Myriam Laly, Patricia Duchamp-Viret, Fabrice Neiers, Gérard Coureaud, Jean-Marie Heydel

1452 related Products with: Molecular and Cellular Characterization of the Glutathione Transferases Involved in the Olfactory Metabolism of the Mammary Pheromone.

1100.00 ul100 U100

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#39440037   2024/10/08 To Up

Cell line-based models of normal and chronic bronchitis-like airway mucosa to study the toxic potential of aerosolized palladium nanoparticles.

Physiologically relevant cell line-based models of human airway mucosa are needed to assess nanoparticle-mediated pulmonary toxicity for any xenbiotics expsoure study. Palladium nanoparticles (Pd-NP) originating from catalytic converters in vehicles pose health risks. We aimed to develop airway models to assess the toxic potential of Pd-NP in normal (Non-CB) and chronic bronchitis-like (CB-like) mucosa models.
Jie Ji, Katja Jansen, Vadim Kessler, Gulaim Seisenbaeva, Per Gerde, Maria Malmlöf, Lena Palmberg, Swapna Upadhyay

1469 related Products with: Cell line-based models of normal and chronic bronchitis-like airway mucosa to study the toxic potential of aerosolized palladium nanoparticles.

500 gm.One 96-Well Microplate Ki1 vialOne 96-Well Microplate Ki1 kit1 mLTwo 96-Well Microplate Ki~2x10(6) cells

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#39407195   2024/10/15 To Up

Microcavity-assisted cloning (MAC) of hard-to-clone HepG2 cell lines: cloning made easy.

Cloning is a key molecular biology procedure for obtaining a genetically homogenous population of organisms or cell lines. It requires the expansion of new cell populations starting from single genetically modified cells. Despite the technical progress, cloning of many cell lines remains difficult. Cloning often fails either due to the strenuous conditions associated with manipulating cells or because many cells don't tolerate a single-cell state. Here we describe a new cloning method utilizing low adhesion microcavity plates. This new technique, named microcavity-assisted cloning (MAC) was developed to clone difficult-to-clone HepG2 cells. The clones were produced following CRISPR/Cas9 knockout of the GSTA1 gene by a random distribution of 200, 400, and 800 cells into 550 microcavities of a 24-well low adhesion plate originally designed for the culture of spheroids. The knockout of GSTA1 was verified at the protein level using Western blotting. The advantages of the MAC method are its low cost, ease of the procedure, and the possibility of scaling up the throughput and automatization.
Vid Mlakar, Laurence Lesne, Stefania Vossio, Isabelle Dupanloup, Yvonne Gloor, Dimitri Moreau, Marc Ansari

2082 related Products with: Microcavity-assisted cloning (MAC) of hard-to-clone HepG2 cell lines: cloning made easy.

20 x 50ul/Unit10 x 50ul/Unit10 rxns1 Plate of 96 x 20ul/Unit5 x 200ul/Unit5 x 200ul/Unit75 x 50ul/Unit 6 ml Ready-to-use 2 ml Ready-to-use 100 Plates

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#39391485   2024/09/18 To Up

Landscape of epithelial cell subpopulations in the human esophageal squamous cell carcinoma microenvironment.

We sought to reveal the landscape of epithelial cell subpopulations in the human esophageal squamous cell carcinoma microenvironment and investigate their parts on esophageal squamous carcinoma (ESCC) development.
Jingrong Yang, Bo Wu, Guo Li, Chenxi Zhang, Yongwei Xie, Wencui Kong, Zhiyong Zeng

2929 related Products with: Landscape of epithelial cell subpopulations in the human esophageal squamous cell carcinoma microenvironment.

96tests96tests

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#39305848   2024/09/20 To Up

IL-17A activates JAK/STAT signaling to affect drug metabolizing enzymes and transporters in HepaRG cells.

The founding family member, Interleukin (IL)-17A, is commonly known as IL-17 and has garnered increasingly attention for proinflammatory functions in autoimmune disorders. Although the effects of IL-17A on hepatic important drug-metabolizing enzymes and transporters (DMETs) expression still remain unclear, it is critical to ascertain owing to the well-established alterations of the drug disposition capacity of the liver occurring during immune imbalance. The present study was designed to explore the effects and mechanisms of IL-17A on DMETs mRNA and protein expression in HepaRG cells by real-time quantitative reverse transcription polymerase chain reaction and Western blot, respectively. It is discovered that IL-17A can inhibit most DMETs mRNA expression (drug-metabolizing enzymes of CYP1A2, CYP3A4, CYP2C9, CYP2C19, GSTA1 and UGT1A1 and transporters of NTCP, OCT1, OATP1B1, BCRP and MDR1) as well as the protein expression of CYP3A4 and CYP2C19, via the janus kinase 2 (JAK2)-signal transducer and activator of transcription 3 (STAT3) signaling pathway. Thus, abnormal regulation of DMETs in IL-17A-mediated immune disorders such as psoriasis may cause alterations in pharmacokinetic processes and may occasionally result in unexpected drug-drug interactions (DDIs) in clinical practice.
Yuanyuan Li, Nan Guo, Yinyu Zhao, Jiali Chen, Jinxia Zhao, Jialu Bian, Jing Guo, Changqing Yang, Xiaohong Zhang, Lin Huang

2559 related Products with: IL-17A activates JAK/STAT signaling to affect drug metabolizing enzymes and transporters in HepaRG cells.

8 inhibitors2 Pieces/Box100 assays1 Set96 wells (1 kit)1.00 flask1.00 flask2 mg1 Set100 assays1 Set

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#39199566   2024/08/08 To Up

Molecular Factors Predicting Ovarian Chemotoxicity in Fertile Women: A Systematic Review.

: Recent advances in cancer diagnosis and treatment have significantly improved survival rates among women of reproductive age facing cancer. However, the potential iatrogenic loss of fertility caused by chemotherapeutic agents underscores the need to understand and predict chemotherapy-induced ovarian damage. This study addresses this gap by systematically reviewing the literature to investigate genetic markers associated with chemotherapy-induced ovarian failure (CIOF). : The primary objective is to identify genetic markers linked to CIOF, contributing to a comprehensive understanding of the factors influencing fertility preservation in female cancer survivors. : A systematic review was conducted using PubMed, EMBASE, Web of Science, Scopus, and OVID electronic databases from inception through December 2023. Studies were included if they featured genomic assessments of genes or polymorphisms related to CIOF in women with histologically confirmed tumors. Exclusion criteria comprised in vitro and animal studies, reviews, and pilot studies. The resulting four human-based studies were scrutinized for insights into genetic influences on CIOF. : Of the 5179 articles initially identified, four studies met the inclusion criteria, focusing on alkylating agents, particularly cyclophosphamide, and anthracyclines. Su et al. explored variants, revealing modified associations with CIOF based on age. Charo et al. investigated and polymorphisms, emphasizing the need to consider age and tamoxifen therapy in assessing associations. Oktay et al. delved into the impact of BRCA mutations on anti-Müllerian hormone (AMH) levels post-chemotherapy, supported by in vitro assays. Van der Perk et al. focused on childhood cancer survivors and revealed significant associations of and SNPs with AMH levels. : This systematic review analyzes evidence regarding genetic markers influencing CIOF, emphasizing the complex interplay of age, specific genetic variants, and chemotherapy regimens. The findings underscore the need for a personalized approach in assessing CIOF risk, integrating genetic markers with traditional ovarian reserve testing. The implications of this study extend to potential advancements in fertility preservation strategies, offering clinicians a comprehensive baseline assessment for tailored interventions based on each patient's unique genetic profile. Further research is essential to validate these findings and establish a robust framework for integrating genetic markers into clinical practice.
Diego Raimondo, Antonio Raffone, Daniele Neola, Federica Genovese, Antonio Travaglino, Alberto Aguzzi, Valeria De Gobbi, Agnese Virgilio, Sara Di Santo, Rossella Vicenti, Valentina Magnani, Maurizio Guida, Tommaso Pippucci, Renato Seracchioli

1764 related Products with: Molecular Factors Predicting Ovarian Chemotoxicity in Fertile Women: A Systematic Review.



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#39199146   2024/07/25 To Up

Turcz. Honey Prevents Inflammation Response and Inhibits Ferroptosis by / Pathway in DSS-Induced Human Caco-2 Cells.

Turcz. () honey, a monofloral honey, has garnered increased attention due to its origin in the plant. A previous study has shown that honey can ameliorate inflammation. In this study, we aimed to investigate the effects of honey extract and its biomarker (Trifolin) on DSS-induced ulcerative colitis (UC). Our results demonstrated that honey extract and Trifolin significantly increased the expression levels of the tight junction cytokines and . Additionally, they decreased the pro-inflammatory factors and and enhanced the antioxidant factors and . Based on metabolomic analyses, honey extract and Trifolin regulated the progression of UC by inhibiting ferroptosis. Mechanistically, they improved the levels of SOD and iron load, increased the GSH/GSSG ratio, reduced MDA content and ROS release, and upregulated the / pathway, thereby inhibiting DSS-induced UC. Moreover, the expression levels of ferroptosis-related genes indicated that they decreased , , and while increasing expression to resist ferroptosis. In conclusion, our study found that honey improves DSS-induced UC by inhibiting ferroptosis by activating the / pathway. These findings further elucidate the understanding of anti-inflammatory and antioxidant activities of honey.
Caijun Ren, Yuying Zhu, Qiangqiang Li, Miao Wang, Suzhen Qi, Dandan Sun, Liming Wu, Liuwei Zhao

1880 related Products with: Turcz. Honey Prevents Inflammation Response and Inhibits Ferroptosis by / Pathway in DSS-Induced Human Caco-2 Cells.

4 Membranes/Box20ug5ug100ug Lyophilized1.00 flask16 Arrays/Slide4 Sample Kit8 Sample Kit4 Membranes/Box 100 UG16 Arrays/Slide

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#39178679   2024/08/17 To Up

Exploring the anti-ovarian aging mechanism of He's Yangchao formula: Insights from multi-omics analysis in naturally aged mice.

The rapid acceleration of female reproductive aging has become a major public health concern. He's Yangchao formula (HSYC), a compound comprising eight herbs, has demonstrated efficacy in enhancing ovarian function. Thus, an in-depth study of its anti-ovarian aging mechanism is required.
Liuqing Yang, Xinle Lai, Shuo Jin, Heng Wang, Fangxuan Lin, Xin Jin, Yun Chen, Ruye Wang, Yun Huang, Yiqun Zhang, Saisai Tian, Xiaohong Fang, Xing Duan, Qin Zhang

2806 related Products with: Exploring the anti-ovarian aging mechanism of He's Yangchao formula: Insights from multi-omics analysis in naturally aged mice.

50 UG100 μg0.1ml100 μg

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#39063019   2024/07/16 To Up

Association of Glutathione Transferase M1, T1, P1 and A1 Gene Polymorphism and Susceptibility to IgA Vasculitis.

Endothelial cell injury is a hallmark of IgA vasculitis (IgAV), possibly associated with various factors, including oxidative stress. Certain single nucleotide polymorphisms (SNPs) of glutathione S-transferases () genes have been shown to increase susceptibility to oxidative stress. The objective of our study was to evaluate the gene polymorphisms of , , and in patients with IgAV. DNA was extracted from the blood of 124 children with IgAV and 168 age-matched healthy controls. A higher frequency of the null genotype was observed in patients with gastrointestinal (GI) system involvement compared to those without GI system involvement (51.5% vs. 28.6%, = 0.011). Additionally, the null genotype was less prevalent (30.8% vs. 69.2%, = 0.032), while the Val/Val genotype was significantly more prevalent in patients who developed urogenital complications (scrotal swelling) during the course of the disease (60% vs. 40%, = 0.039). This study is the first to suggest an association between and polymorphisms and various phenotypes observed during the clinical course of IgAV in the pediatric population. However, it was performed on a national and likely single ethnic cohort, too small for definitive conclusions, so larger studies are needed to confirm this association.
Ana Juras, Kristina Crkvenac Gornik, Martina Held, Mario Sestan, Daniel Turudic, Matej Sapina, Sasa Srsen, Sanda Huljev Frkovic, Marijan Frkovic, Alenka Gagro, Marija Jelusic

1712 related Products with: Association of Glutathione Transferase M1, T1, P1 and A1 Gene Polymorphism and Susceptibility to IgA Vasculitis.

25 mg 100ul 5 G 5 G50 ug 100ul1 g2.5 mg96 wells (1 kit)100ug1x96 well plate100 assays

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#39042459   2024/07/23 To Up

Transcriptional analysis of primary ciliary dyskinesia airway cells reveals a dedicated cilia glutathione pathway.

Primary ciliary dyskinesia (PCD) is a genetic condition that results in dysmotile cilia. The repercussions of cilia dysmotility and gene variants on the multiciliated cell remain poorly understood. We used single-cell RNA-Seq, proteomics, and advanced microscopy to compare primary culture epithelial cells from patients with PCD, their heterozygous mothers, and healthy individuals, and we induced pluripotent stem cells (iPScs) generated from a patient with PCD. Transcriptomic analysis revealed unique signatures in PCD airway cells compared with their mothers' cells and the cells of healthy individuals. Gene expression in heterozygous mothers' cells diverged from both control and PCD cells, marked by increased inflammatory and cellular stress signatures. Primary and iPS-derived PCD multiciliated cells had increased expression of glutathione-S-transferases GSTA2 and GSTA1, as well as NRF2 target genes, accompanied by elevated levels of reactive oxygen species (ROS). Immunogold labeling in human cilia and proteomic analysis of the ciliated organism Chlamydomonas reinhardtii demonstrated that GSTA2 localizes to motile cilia. Loss of human GSTA2 and C. reinhardtii GSTA resulted in slowed cilia motility, pointing to local cilia regulatory roles. Our findings identify cellular responses unique to PCD variants and independent of environmental stress and uncover a dedicated ciliary GSTA2 pathway essential for normal motility that may be a therapeutic target.
Jeffrey R Koenitzer, Deepesh Kumar Gupta, Wang Kyaw Twan, Huihui Xu, Nicholas Hadas, Finn J Hawkins, Mary Lou Beermann, Gervette M Penny, Nathan T Wamsley, Andrew Berical, Michael B Major, Susan K Dutcher, Steven L Brody, Amjad Horani

1168 related Products with: Transcriptional analysis of primary ciliary dyskinesia airway cells reveals a dedicated cilia glutathione pathway.

1.5x10(6) cells 100ul2 Pieces/Box1 module500 100 µg25 100 100ug

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