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#33094105   2020/09/17 To Up

Laboratory Diagnosis of a NZ7-like Orf Virus Infection and Pathogen Genetic Characterization, Particularly in the Gene.

Orf is a widespread contagious epithelial viral disease found particularly in most sheep breeding countries in the world. Recently, an orf virus (ORFV) strain OV-HLJ05 was isolated from an outbreak in northeast China. Three genes of interest including ORFV011 (B2L), ORFV059 (F1L), and ORFV132 (VEGF) of ORFV, were recruited to identify and genetically characterize this newly isolated virus. Amino acid (aa) sequence compared with the ORFV references listed in GenBank, both B2L and F1L of OV-HLJ05 showed less microheterogeneity from their references. In contrast, the VEGF gene was included in the NZ7-VEGF like group as previously considered by Mercer in 2002. Unexpectedly, further multiple VEGF matches were made, using 34 published sequences from China and India, resulting in 27 strains of the NZ7 members. Based on Karki's report in 2020, NZ7-VEGF like viruses are emerging more and more frequently in these two countries, damaging the Asian sheep industry. Obvious heterogeneity with the NZ2, insertion of two oligopeptides TATI(L)QVVVAI(L) and SSSS(S) motif were found in the NZ7-like VEGF protein. These VEGFs are divided mainly into two types and a significant increase in the number of hydrogen bonds within the NZ7-like VEGF dimers was observed. The NZ7-like ORFV apparently favors the goat as a host and an emphasis on this in future epidemiological and pathological studies should be considered, focusing on the NZ7-like virus.
Yongzhong Yu, Xuyang Duan, Yuanyuan Liu, Jinzhu Ma, Baifen Song, Zhengxing Lian, Yudong Cui

1119 related Products with: Laboratory Diagnosis of a NZ7-like Orf Virus Infection and Pathogen Genetic Characterization, Particularly in the Gene.

1001 mg1 mg100 251 mg100 2ug1001 mL

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#33093236   2020/10/22 To Up

High-density lipoprotein-associated miRNA is increased following Roux-en-Y gastric bypass surgery for severe obesity.

Roux-en-Y gastric bypass (RYGB) is one of the most commonly performed weight-loss procedures, but how severe obesity and RYGB affects circulating HDL-associated microRNAs (miRNAs) remains unclear. Here, we aim to investigate how HDL-associated miRNAs are regulated in severe obesity and how weight loss after RYGB surgery affects HDL-miRNAs. Plasma HDL were isolated from patients with severe obesity (n=53) before, 6 and 12 months after RYGB by immunoprecipitation using goat anti-human apoA-I microbeads. HDL were also isolated from 18 healthy participants. miRNAs were extracted from isolated HDL and levels of miR-24, miR-126, miR-222 and miR-223 were determined by TaqMan miRNA assays. We found that HDL-associated miR-126, miR-222 and miR-223 levels, but not miR-24 levels, were significantly higher in patients with severe obesity when compared with healthy controls. There were significant increases in HDL-associated miR-24, miR-222 and miR-223 at 12 months after RYGB. Additionally, cholesterol efflux capacity and paraoxonase (PON1) activity were increased and intracellular adhesion molecule-1 (ICAM-1) levels decreased. The increases in HDL-associated miR-24 and miR-223 were positively correlated with increase in cholesterol efflux capacity (r=0.326, P=0.027 and r=0.349, P=0.017 respectively). An inverse correlation was observed between HDL-associated miR-223 and ICAM-1 at baseline. Together, these findings show that HDL-associated miRNAs are differentially regulated in healthy versus patients with severe obesity and are altered after RYGB. These findings provide insights into how miRNAs are regulated in obesity before and after weight reduction, and may lead to the development of novel treatment strategies for obesity and related metabolic disorders.
Jan Hoong Ho, Kwok Leung Ong, Luisa F Cuesta Torres, Yifen Liu, Safwaan Adam, Zohaib Iqbal, Shaishav Dhage, Basil J Ammori, Akheel A Syed, Kerry-Anne Rye, Fatiha Tabet, Handrean Soran

1068 related Products with: High-density lipoprotein-associated miRNA is increased following Roux-en-Y gastric bypass surgery for severe obesity.

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#33092682   2020/10/23 To Up

Prevalence and genotyping of sensu lato from livestock in north-eastern Kenya.

Cystic echinococcosis (CE) is a zoonotic disease of cosmopolitan distribution and caused by the larval stage of the dog tapeworm, Echinococcus granulosus sensu lato (s.l.). CE occurs in the wider African continent and in Kenya, notably in the Maasailand and Turkana regions; however, recent studies demonstrate its presence in other parts of Kenya. This study determined the occurrence of CE in livestock (camels, goats, sheep and cattle) in Isiolo, Garissa and Wajir counties, and characterized the species of E. granulosus s.l. present. An abattoir survey was used to determine the presence of CE in various organs in livestock. Polymerase chain reaction-restriction fragment length polymorphism and sequencing of the mitochondrial NADH dehydrogenase subunit 1 gene was used for genotyping. A total of 1368 carcasses from 687 goats, 234 camels, 329 sheep and 118 cattle were inspected for the presence of hydatid cysts. The overall proportion of infections was 29.1% in camels, 14.4% in cattle, 9.9% in goats and 8.2% in sheep. The liver was the most infected organ, while only the lung of camels harboured fertile cysts. Of the 139 cysts genotyped, 111 (79.9%) belonged to Echinococcus canadensis (G6/7) and 20 (14.4%) to E. granulosus sensu stricto. One and two cysts were identified as Taenia saginata and unknown Taenia species, respectively. There was a significant association between county of origin and species of the animal with occurrence of CE. This study reports, for the first time, the characterization of Echinococcus species in livestock from Garissa and Wajir counties, and the current situation in Isiolo county. The fertility of cysts in camels and frequency of E. canadensis (G6/7) in all livestock species indicate that camels play an important role in the maintenance of CE in the north-eastern counties of Kenya.
H A Omondi, G Gitau, P Gathura, E Mulinge, E Zeyhle, P Kimeli, B Bett

2646 related Products with: Prevalence and genotyping of sensu lato from livestock in north-eastern Kenya.

7 x 25 ul5mg100ug 5 G5 mg100ug100ug100ul1 Set11 Set

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#33091227   2020/10/22 To Up

A retrospective study (2007-2015) on brucellosis seropositivity in livestock in South Africa.

In South Africa, brucellosis testing and record-keeping are done by several laboratories, thus it is difficult to access any organized data to assess the status of the disease. This study evaluated the seropositivity for brucellosis using Rose Bengal test and complement fixation test in suspect cattle, sheep, goats and pigs sera submitted to Bacterial Serology Laboratory, Agricultural Research Council-Onderstepoort Veterinary Research (ARC-OVR) from nine provinces in the country during the period 2007-2015. This retrospective data analysis was conducted to estimate the occurrence of brucellosis in the country from the submitted samples, identify variables that affected seropositivity for brucellosis, investigate existing gaps in data recording and make recommendations on important variables to facilitate better data capture and inferences on brucellosis. Nine years of data were collated and analysed to detect association (seropositivity over time regarding animal species and location). Of the 764,276 animals tested, the distribution of samples was 90.50% (691,539/764,276), 5.19% (39,672/764,276), 3.92% (29,967/764,276) and 0.41% (3,098/764,276) for cattle, sheep, goats and pigs, respectively. The seropositivity for brucellosis by animal species was 6.31% (43,666/691,539, 95% CI: 6.26-6.37), 2.09% (828/39,672, 95% CI: 1.95-2.23), 0.63% (189/29,967, 95% CI: 0.55-0.73) and 0.13% (4/3,098, 95% CI: 0.05-0.33) in cattle, sheep, goats and pigs respectively. The data available did not capture information on the age, sex, breed and other host risk factors that would have been related to seropositivity for brucellosis. The data provide an understanding of the disease occurrence and confirm that brucellosis is enzootic in South Africa. Improved and standardized data collection can be used to pro-actively drive, monitor, change or formulate policies to mitigate the challenges brought about by brucellosis in the livestock sector in South Africa.
Francis B Kolo, Abiodun A Adesiyun, Folorunso O Fasina, Andrew Potts, Banenat B Dogonyaro, Charles T Katsande, Henriette Van Heerden

1833 related Products with: A retrospective study (2007-2015) on brucellosis seropositivity in livestock in South Africa.

100ug100μg100 ug100ug Lyophilized100 μg100ug Lyophilized100 μg1 Set100 μg1 Set4 Membranes/Box100 μg

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#33087950   2020/04/28 To Up

Retention of vitamin A after goat milk processing into cheese: a nutritional strategy.

The deficiency in micronutrients is a public health problem, principally in lower-middle-income countries. Vitamin A (VA) is considered a micronutrient fundamental to the maintenance and development of different tissues in the organism. Therefore, it is an essential micronutrient in the human diet. In these terms, goat milk is the leading food consumed to provide nutritional support in innumerous lower-middle-income countries. Here our work aimed to produce goat cheese studying strategies to promote the retention of VA. Our experiment design also explores the use of the salting process to evaluate the levels of VA retention. The level of VA in goat cheese was determined using LC-MS/MS analysis. Additionally, the redox status of the goat cheese in terms of lipid peroxidation and protein oxidation was determined. The texture analysis was also evaluated to verify if the redox status and salting process influence the texture profile. The results showed that the salting process during goat cheese production improves the retention of VA in goat cheese. Moreover, the salting process also is related to alterations in the status redox of the goat cheese and texture parameters. Therefore, our results show that goat cheese production can be an alternative to produced dairy derivates with recognized concentrations of VA for human nutrition.
Suelma Ferreira do Oriente, Fabiano Barreto, Caroline Andrade Tomaszewski, Luca Suchecki Barnet, Natália Cabral Souza, Hugo Miguel Lisboa Oliveira, Matheus Augusto de Bittencourt Pasquali

2518 related Products with: Retention of vitamin A after goat milk processing into cheese: a nutritional strategy.

100 μg100 μg100 μg1L100 μg2ml500 100 μg100 0.1 mg0.1 mg0.1 mg

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#33087469   2020/10/21 To Up

The NSs protein encoded by the virulent strain of Rift Valley fever virus targets the expression of Abl2 and the actin cytoskeleton of the host affecting cell mobility, cell shape and cell-cell adhesion.

Rift Valley fever virus (RVFV) is a highly pathogenic zoonotic arbovirus endemic in many African countries and the Arabian Peninsula. Animal infections cause high rates of mortality and abortion among sheep, goats and cattle. In humans, an estimated 1-2% of RVFV infections result in severe disease (encephalitis, hepatitis, retinitis) with a high rate of lethality when associated to hemorrhagic fever. RVFV's NSs protein, which is RVFV's main factor of virulence, counteracts the host innate antiviral response favoring viral replication and spread. However, the mechanisms underlying RVFV-induced cytopathic effects and the role of NSs in these alterations remain for the most undeciphered. In this work we have analyzed the effects of NSs expression on actin cytoskeleton while conducting infections with the NSs expressing virulent (ZH548) and attenuated (MP12) strains of RVFV and the non-NSs expressing avirulent (ZH548ΔNSs) strain as well as after the ectopic expression of NSs. In macrophages, fibroblasts and hepatocytes NSs expression prevented the up-regulation of Abl2 (a major regulator of actin cytoskeleton) expression otherwise induced by avirulent infections and identified here as part of the antiviral response. The presence of NSs was also linked to and increased mobility of ZH548- as compared to ZH548ΔNSs-infected fibroblasts and to strong changes in cell morphology in non-migrating hepatocytes with reduction of lamellipodia, cell spreading and dissolution of adherens junctions reminiscent of ZH548-induced cytopathic effects observed Finally, we show evidence of the presence of NSs within long actin-rich structures associated to NSs dissemination from NSs expressing towards non-NSs expressing cells. Rift Valley fever virus (RVFV) is a dangerous human and animal pathogen that was ranked in 2018 by the World Health Organization among the eight pathogens of most concern likely to cause wide epidemics in the near future for which there is no, or insufficient, countermeasures. The interest of this work resides in the fact that it addresses the question of the mechanisms underlying RVFV-induced cytopathic effects that participate in RVFV's pathogenicity. We demonstrate here that RVFV targets cell adhesion and actin cytoskeleton at the transcriptional and cellular level, affecting cell mobility and inducing cell shape collapse alongside with distortion of cell-cell adhesion. All these effects are susceptible to participate in RVFV-induced pathogenicity, facilitate virulent RVFV dissemination and thus constitute interesting potential targets in future development of antiviral therapeutic strategies that in the case of RVFV, as several other emerging arboviruses, are presently lacking.
Aline Bamia, Vasco Marcato, Magali Boissière, Zeyni Mansuroglu, Carole Tamietti, Mattea Romani, Dominique Simon, Guanfang Tian, Florence Niedergang, Jean-Jacques Panthier, Marie Flamand, Sylvie Souès, Eliette Bonnefoy

1625 related Products with: The NSs protein encoded by the virulent strain of Rift Valley fever virus targets the expression of Abl2 and the actin cytoskeleton of the host affecting cell mobility, cell shape and cell-cell adhesion.

100 U24 reactions 1mg10

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#33086185   2020/10/15 To Up

Interaction between abomasal blood feeder Haemonchus contortus and intestinal mucosal browser Trichostrongylus colubriformis during co-infection in Boer goats.

This study was conducted to investigate potential interactions between the abomasal blood feeder Haemonchus contortus and the intestinal mucosal browser Trichostrongylus colubriformis among deliberately infected Boer goats. Faecal and blood samples were collected twice a week for eight weeks from 25 parasite-naïve goats. Correlation analysis and multiple linear regression models were conducted to explore the association between phenotypic variables and variables taken at necropsy. Positive associations were identified between total FEC and log T. colubriformis number (r = 0.62, p < 0.05) as well as between IgA and peripheral eosinophil counts (r = 0.65, p < 0.05). A negative correlation was observed between T. colubriformis and log H. contortus number (r=-0.56, p < 0.05). Multiple linear regression models show that H. contortus and T. colubriformis interacted with each other. T. colubriformis appeared to contribute more significantly to the variation of FEC than H. contortus. Co-infection induced an IgA response which was only effective against T. colubriformis but not protective against H. contortus infection. This could be seen via significant associations of IgA with both nematode species but with the effect of IgA differing for H. contortus and T. colubriformis. In this study, H. contortus infection was not detrimental to the goats with no observed impact on PCV. This could be because the growth of T. colubriformis as represented by its length was associated with reduced number and composite burden of H. contortus during co-infection, or possibly due to low infection dosage. Improved understanding of the impact of H. contortus and T. colubriformis and their interaction from natural co-infection studies is beneficial for a better understanding of the goat-parasite interaction and its potential impacts on the health and productivity of animals.
N Hayyan Basripuzi, Reuben S K Sharma, Z Norhadila, Z S Shahar, M S Nor-Dilaila, M S Mustapha, V Kolandaiveloo, K Ruviniyia, L Matthews

2460 related Products with: Interaction between abomasal blood feeder Haemonchus contortus and intestinal mucosal browser Trichostrongylus colubriformis during co-infection in Boer goats.

100ug Lyophilized1 Set1 Set1 Set1 Set1 Set1 Set1 Set1 Set1 Set1 Set1 Set

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#33083929   2020/10/20 To Up

Genetic Characterization of Echinococcus granulosus Sensu Lato in Livestock and Human Isolates from North of Iran Indicates the Presence of E. ortleppi in Cattle.

Identification of different genotypes of echinococcal cyst in various domestic herbivores and humans within the target area was the principal aim of the present study, performed using sequence data of cox1 and nad1 mitochondrial genes.
Khadijeh Nematdoost, Keyhan Ashrafi, Bijan Majidi-Shad, Eshrat Beigom Kia, Arash Zeinali, Meysam Sharifdini

2939 related Products with: Genetic Characterization of Echinococcus granulosus Sensu Lato in Livestock and Human Isolates from North of Iran Indicates the Presence of E. ortleppi in Cattle.

96 tests96 tests96 tests96 tests100 μg2 100 μg100 units100 μg100 μg100 μg

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