Search results for: HRP
#35039290 2022/01/17 To Up
Association between prelabour caesarean section and perinatal outcomes: analysis of demographic and health surveys from 26 low-income and middle-income countries.Caesarean section (CS) conducted before labour (prelabour CS (PLCS)), compared with vaginal birth, may pose additional maternal and perinatal risks. No multicountry analysis has examined PLCS in low-income and middle-income countries (LMICs). This study assessed rates, risk factors and associations of PLCS with perinatal outcomes in LMICs.
Newton Opiyo, Saverio Bellizzi, Maria Regina Torloni, Joao Paulo Souza, Ana Pilar Betran
2365 related Products with: Association between prelabour caesarean section and perinatal outcomes: analysis of demographic and health surveys from 26 low-income and middle-income countries.10 mg200ug1000 TESTS/0.65ml25 mg1000 tests100ug200ul100 mg50 ug 10 mg100ug
#35035095 2022/01/11 To Up
Unified-amplifier based primer exchange reaction (UniAmPER) enabled detection of SARS-CoV-2 from clinical samples.Primer exchange reaction (PER) is an emergent method for non-templated synthesis of single stranded DNA molecules. PER has been shown to be effective in cell imaging systems and for detection of macromolecules. A particular application of PER is to detect a specific target nucleic acid. To this endeavor, two coupled DNA hairpins, a detector and an amplifier, play in accordance to extend a target nucleic acid with a concatemer DNA sequence. Here we introduced unified-amplifier based primer exchange reaction (UniAmPER) that beneficially extends the target by a unified-amplifier. The unified-amplifier operates as both detector and amplifier hairpins. The extension resulted in synthesis of concatemer G-rich sequences. The G-rich sequences were expected to form G-quadruplex (GQ) structures. Presence of the GQ structures were investigated by peroxidase activity of GQs in presence 22 generation upon intercalation of thioflavin T (ThT). The presented unified-amplifier in this study facilitates application of PER systems for development of colorimetric or fluorogenic biosensors. As a proof of principle, the method has been applied for detection of reversely transcribed cDNAs from clinical SARS-CoV-2 samples.
Reyhaneh Tavakoli-Koopaei, Fatemeh Javadi-Zarnaghi, Hossein Mirhendi
2608 related Products with: Unified-amplifier based primer exchange reaction (UniAmPER) enabled detection of SARS-CoV-2 from clinical samples.4X1.25ml; 200 reactions (2x96 well plate2x96 well plate2x384 well plate2000 Units4X1.25ml; 200 reactions (2x96 well plates200ul4X1.25ml; 200 reactions (2x96 well plate250tests
#35030500 2022/01/10 To Up
Enrichment of IgG and HRP glycoprotein by dipeptide-based polymeric material.Separation, purification, and identification of glycoproteins are essential for understanding their vital roles in biological and pathological processes. However, glycoproteins are difficult to be captured due to their low abundance, strong interference from non-glycosylated proteins. Here, we report a promising dipeptide-based saccharide recognition platform to selectively enrich two typical glycoproteins, named immunoglobin G (IgG) and horseradish peroxidase (HRP). Different from the conventional glycoprotein enrichment method based on boronic acid affinity or hydrophilic interaction with glycans, the present method was established based on affinity between Pro-Glu (PE) dipeptide and mannose, which is a key unit in the pentasaccharide core of the IgG and HRP glycans. The prepared PE homopolymer surface was proved to selectively bind IgG and HRP superior to that of bovine serum albumin (BSA). Benefiting from this feature, selective enrichment of IgG and HRP was achieved from a protein mixture containing 200-fold BSA interference by using [email protected] under a dispersive solid-phase extraction (dSPE) mode. High adsorption capacity, controllable and selective adsorption behaviors, as well as satisfactory recovery demonstrated the high potential of the dipeptide-based polymeric material in IgG and HRP enrichment. This study might provide a new insight to solve the challenging problem of glycoprotein separation.
Xintong Zheng, Fusheng Zhang, Yanyan Zhao, Yuting Xiong, Xiaoyu Zhang, Zhenqiang Shi, Shengxu Qian, Haijuan Qin, Guangyan Qing
2544 related Products with: Enrichment of IgG and HRP glycoprotein by dipeptide-based polymeric material.100 ul100 ul100 ul100 ul0.1ml (1mg/ml)100 ul100μg100 ul1 mg100 ug0,25ml / 50 test
#35029790 2022/01/14 To Up
A Distance-Based Microfluidic Paper-Based Biosensor for Glucose Measurements in Tear Range.The prevalence of diabetes has increased over the past years. Therefore, developing minimally invasive, user-friendly, and cost-effective glucose biosensors is necessary especially in low-income and developing countries. Cellulose paper-based analytical devices have attracted the attention of many researchers due to affordability, not requiring trained personnel, and complex equipment. This paper describes a microfluidic paper-based analytical device (Î¼PAD) for detecting glucose concentration in tear range with the naked eye. The paper-based biosensor fabricated by laser CO2; and glucose oxidase/horseradish peroxidase (GOx/HRP) enzyme solution coupled with tetramethylbenzidine (TMB) were utilized as reagents. A sample volume of 10Â Î¼l was needed for the biosensor operation and the results were observable within 5Â min. The color intensity-based and distance-based results were analyzed by ImageJ and Tracker to evaluate the device performance. Distance-based results showed a linear behavior in 0.1-1.2Â mM with an Râ=â0.9962 and limit of detection (LOD) of 0.1Â mM. The results could be perceived by the naked eye without needing additional equipment or trained personnel in a relatively short time (3-5Â min).
Samira Allameh, Mohsen Rabbani
1363 related Products with: A Distance-Based Microfluidic Paper-Based Biosensor for Glucose Measurements in Tear Range.1,000 tests2 Pieces/BoxOne 96-Well Microplate Ki100tests96 assays500 tests 1 G
#35026727 2022/01/17 To Up
A Novel Malaria Lateral Flow Assay for Detecting Plasmodium falciparum Lactose Dehydrogenase in Busia, Uganda.Rapid diagnostic tests (RDTs) for Plasmodium falciparum commonly detect histidine-rich protein 2 (HRP-2), but HRP-2 deletions are increasingly recognized. We evaluated a prototype test detecting parasite lactate dehydrogenase (pLDH) and compared it to commercially available RDTs at a health facility in Uganda, using quantitative polymerase chain reaction as a gold standard. The prototype pLDH test had a high sensitivity for infections with at least 100 parasites/ÂµL (98%), comparable to HRP-2, and greater than an existing pLDH RDT (89%). Specificity for the prototype test was 99.5%, which is greater than the HRP-2 tests (93-95%). Therefore, the prototype pLDH test may be an attractive alternative malaria diagnostic.
Christine M Bachman, David M Cate, Ben Grant, Stephen Burkot, Jerry Mulondo, Helen V Hsieh, Martin Chamai, Bakar Odongo, Peter Olwoch, Mayimuna Nalubega, Harriet Ochokoru, Joseph Kasozi, John Ategeka, Kevin P Nichols, Bernhard H Weigl, Bryan Greenhouse
2335 related Products with: A Novel Malaria Lateral Flow Assay for Detecting Plasmodium falciparum Lactose Dehydrogenase in Busia, Uganda.1 mg1 mg1 kit1 kit100 assays0.1ml (1mg/ml)96 Tests1 kit100 assays100 assays100ug Lyophilized
#35026636 2021/11/26 To Up
Highly-sensitive and simple fluorescent aptasensor for 17Â b-estradiol detection coupled with HCR-HRP structure.As an important kind of environmental endocrine disruptors, 17 Î² -Estradiol (E2) plays a major role in affecting the growth of human including sexual characters, pregnancy system, etc. In the modern society, with the threat of abuse in breeding, it is imperative to design sensitive methods for detecting low concentration of E2 in environment. In this work, we constructed a highly sensitive and simple fluorescent aptasenor for detecting E2 via amplification of hybridization chain reaction (HCR) and horseradish peroxidase (HRP). Through the competitions between complementary strand (cmDNA) and E2 to E2 aptamer modified on magnetic beads, the unbound cmDNA would be collected and captured by polystyrene microspheres to induce HCR which brought abundant biotin sites. Subsequently, benefit from the excellent catalytic performance of streptavidin-horseradish peroxidase (SA-HRP), the highly sensitive fluorescence signals could be obtained in low concentration of E2. Under the optimal conditions, the prospered method for E2 detection was shown a good liner range from 1 to 100Â pg/mL, with the lower detecting limit of 0.2Â pg/mL compared with previous work. In addition, the recovery rates tested in the real samples of milk and water were 99.20%-108.06% and 91.07%-106.13%. In all, the assay may provide a perspective way for highly sensitive detection for various contaminants in the real samples.
Hao Yang, Danke Xu
2060 related Products with: Highly-sensitive and simple fluorescent aptasensor for 17Â b-estradiol detection coupled with HCR-HRP structure.96 tests2 x 96 well plate5x96 well plate2x96 well plate
#35024600 2021/12/29 To Up
Injectable hyaluronic acid hydrogel loaded with BMSC and NGF for traumatic brain injury treatment.Injectable hydrogel has the advantage to fill the defective area and thereby shows promise as therapeutic implant or cell/drug delivery vehicle for tissue repair. In this study, an injectable hyaluronic acid hydrogel dual-enzymatically cross-linked by galactose oxidase (GalOx) and horseradish peroxidase (HRP) was synthesized and optimized, and the therapeutic effect of this hydrogel encapsulated with bone mesenchymal stem cells (BMSC) and nerve growth factors (NGF) for traumatic brain injury (TBI) mice was investigated. Results from experiments showed that either tyramine-modified hyaluronic acid hydrogels (HT) or NGF loaded HT hydrogels (HT/NGF) possessed good biocompatibility. More importantly, the HT hydrogels loaded with BMSC and NGF could facilitate the survival and proliferation of endogenous neural cells probably by neurotrophic factors release and neuroinflammation regulation, and consequently improved the neurological function recovery and accelerated the repair process in a C57BL/6 TBI mice model. All these findings highlight that this injectable, BMSC and NGF-laden HT hydrogel has enormous potential for TBI and other tissue repair therapy.
Luyu Wang, Dan Zhang, Yikun Ren, Shen Guo, Jinrui Li, Shanshan Ma, Minghao Yao, Fangxia Guan
1026 related Products with: Injectable hyaluronic acid hydrogel loaded with BMSC and NGF for traumatic brain injury treatment.10 mg10 mg1 ml 1 G 1 G 1 G 1 G 1 G25 g 1 G 500 G10 mg
#35024200 2021/03/20 To Up
Fangchinoline diminishes STAT3 activation by stimulating oxidative stress and targeting SHP-1 protein in multiple myeloma model.The development of cancer generally occurs as a result of various deregulated molecular mechanisms affecting the genes that can control normal cellular growth. Signal transducer and activator of transcription 3 (STAT3) pathway, once aberrantly activated can promote carcinogenesis by regulating the transcription of a number of oncogenic genes.
Young Yun Jung, In Jin Ha, Jae-Young Um, Gautam Sethi, Kwang Seok Ahn
2207 related Products with: Fangchinoline diminishes STAT3 activation by stimulating oxidative stress and targeting SHP-1 protein in multiple myeloma model.100μg100μg100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug100ug Lyophilized61000 100ul1 Set100ug Lyophilized
#35017226 2022/01/11 To Up
Reducing the harms of unsafe abortion: a systematic review of the safety, effectiveness and acceptability of harm reduction counselling for pregnant persons seeking induced abortion.Globally, access to safe abortion is limited. We aimed to assess the safety, effectiveness and acceptability of harm reduction counselling for abortion, which we define as the provision of information about safe abortion methods to pregnant persons seeking abortion.
Bianca Maria Stifani, Roopan Gill, Caron Rahn Kim
2505 related Products with: Reducing the harms of unsafe abortion: a systematic review of the safety, effectiveness and acceptability of harm reduction counselling for pregnant persons seeking induced abortion.100.00 ul1 ml100 100.00 ul1
#35016792 2022/01/08 To Up
Development of a novel molecular assay based on conventional PCR liquid hybridization and ELISA (NAT-ELISA) for the detection of HIV, HCV, and HBV in blood donors.Development of a simple, affordable, and sensitive method based on gene amplification by PCR followed by liquid hybridization for detection of HIV-1 & 2, HCV and HBV in plasma samples (NAT-ELISA) for detection of these viruses particularly in their window period.
Priyanka Sharma, Gunjan Batheja, Hriday Narayan Verma, Pradeep Seth
2575 related Products with: Development of a novel molecular assay based on conventional PCR liquid hybridization and ELISA (NAT-ELISA) for the detection of HIV, HCV, and HBV in blood donors.96 assays96 assays96T96 wells (1 kit)100ug Lyophilized96 tests96T96T0.1ml (1mg/ml)96 wells (1 kit)
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