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#32770861   2020/08/08 To Up

Design of a Fe S Cluster into the Core of a De Novo 4-Helix Bundle.

We explore the capacity of the de novo protein, S824, to incorporate a multinuclear iron-sulfur cluster within the core of a single-chain 4-helix bundle. This topology has a high intrinsic designability because sequences are constrained largely by the pattern of hydrophobic and hydrophilic amino acids, thereby allowing for the extensive substitution of individual side chains. Libraries of novel proteins based on these constraints have surprising functional potential and have been shown to complement the deletion of essential genes in E. coli. Our structure-based design of four first-shell cysteine ligands, one per helix, in S824 resulted in successful incorporation of a cubane Fe S cluster into the protein core. A number of challenges were encountered during the design and characterization process, including non-specific metal-induced aggregation, and the presence of competing metal-cluster stoichiometries. The introduction of buried iron-sulfur clusters into the helical bundle is an initial step toward converting libraries of designed structures into functional de novo proteins with catalytic or electron-transfer functionalities. This article is protected by copyright. All rights reserved.
Joshua A Mancini, Douglas H Pike, Alexei M Tyryshkin, Liti Haramaty, Michael Wang, Saroj Poudel, Michael Hecht, Vikas Nanda

2561 related Products with: Design of a Fe S Cluster into the Core of a De Novo 4-Helix Bundle.



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#32770854   2020/08/08 To Up

Clinical trial to test the efficacy of melatonin in COVID-19.

The pharmacological properties of melatonin are well known. However, there is noticeable the lack of clinical trials that confirm the efficacy, security, absence of side effects in the short and long term, and the effective doses of melatonin. This point is especially important in diseases with high morbidity and mortality including COVID-19. There is not treatment for COVID-19, and several anti-inflammatory and antiviral molecules are being tested, and different vaccines are in preparation. Although the SARS-CoV-2 pandemic is apparently improving, it is expected new resurges next fall. Thus, looking for an effective treatment of COVID-19 is mandatory. Melatonin has significant anti-inflammatory, antioxidant, and mitochondrial protective effects, and its efficacy has been demonstrated in multiple experimental models of disease and in a clinical trial in sepsis. Because COVID-19 courses with a severe septic response, multiple reviews proposing melatonin as a treatment for COVID-19 have been published. Nevertheless, there is a lack of experimental and clinical data on the use of melatonin on SARS-CoV-2 infection. Accordingly, we designed a clinical trial with an injectable formulation of melatonin for intravenous perfusion in ICU patients suffering from COVID-19 that has been just approved by the Spanish Agency of Medicines and Medical Devices (AEMPS). The trial will allow by the first time understand the doses and efficacy of melatonin against COVID-19.
Dario Acuña-Castroviejo, Germaine Escames, Juan C Figueira, Pedro de la Oliva, Alberto M Borobia, Carlos Acuña-Fernández

2573 related Products with: Clinical trial to test the efficacy of melatonin in COVID-19.



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#32770852   2020/08/08 To Up

Slot blotting and flow cytometry: two efficient assays for platelet antibody screening among patients with platelet refractoriness.

Frequent platelet transfusion may lead to the formation of alloantibodies and immune-mediated platelet destruction. Currently, identifying economic and effective screening methods is necessary for the management of platelet transfusion while different tests were recommended. The present study aims to challenge the performance of slot blotting (SB) and flow cytometry (FC) assays in detecting immune platelet refractoriness.
Zahra Ameri, Reza Vahidi, Morteza Khaleghi, Tania Dehesh, Amir Sheikhbardsiri, Alireza Farsinejad

2404 related Products with: Slot blotting and flow cytometry: two efficient assays for platelet antibody screening among patients with platelet refractoriness.

100ul1 kit25 µg100μg100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized4 Sample Kit100ul

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#32770848   2020/08/08 To Up

Spinal Cord Stimulation Attenuates Below-Level Mechanical Hypersensitivity in Rats After Thoracic Spinal Cord Injury.

The burden of pain after spinal cord injury (SCI), which may occur above, at, or below injury level, is high worldwide. Spinal cord stimulation (SCS) is an important neuromodulation pain therapy, but its efficacy in SCI pain remains unclear. In SCI rats, we tested whether conventional SCS (50 Hz, 80% motor threshold [MoT]) and 1200 Hz, low-intensity SCS (40% MoT) inhibit hind paw mechanical hypersensitivity, and whether conventional SCS attenuates evoked responses of wide-dynamic range (WDR) neurons in lumbar spinal cord.
Wanru Duan, Qian Huang, Fei Yang, Shao-Qiu He, Yun Guan

2778 related Products with: Spinal Cord Stimulation Attenuates Below-Level Mechanical Hypersensitivity in Rats After Thoracic Spinal Cord Injury.

30 reactions1 5 MG11ml96T1.00 flask

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#32770845   2020/08/08 To Up

Development and validation of a Score to Assess Risk of Medication Errors detected during medication reconciliation process at admission in Internal Medicine Unit: SCOREM study.

Medication errors (ME) can be reduced through preventive strategies such as medication reconciliation. Such strategies are often limited by human resources and need targeting high risk patients.
Y Audurier, C Roubille, F Manna, L Zerkowski, M Faucanie, V Macioce, A Castet-Nicolas, A Jalabert, M Villiet, P Fesler, L Lohan-Descamps, C Breuker

2346 related Products with: Development and validation of a Score to Assess Risk of Medication Errors detected during medication reconciliation process at admission in Internal Medicine Unit: SCOREM study.

100 μg100 μg100 μg100 μg100 μg100 μg100 μg

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#32770844   2020/08/08 To Up

Metabolomics signature of the seminal plasma in men with severe oligoasthenospermia.

Male factor is incriminated in approximately 50% of cases of infertility. The metabolomics approach has recently been used in the assessment of sperm quality and male fertility.
Magalie Boguenet, Cinzia Bocca, Pierre-Emmanuel Bouet, Orianne Serri, Stéphanie Chupin, Lydie Tessier, Odile Blanchet, Hady El Hachem, Juan Manuel Chao de la Barca, Pascal Reynier, Pascale MayPanloup

1617 related Products with: Metabolomics signature of the seminal plasma in men with severe oligoasthenospermia.

0.05 mg1 mg96T0.05 mg96T

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#32770830   2020/08/08 To Up

Infertility duration and preoperative sperm progressive motility are significant factors of spontaneous pregnancy after varicocele repair.

Varicocele may lead to testicular dysfunction and male infertility. Varicocelectomy can improve both semen quality and postoperative spontaneous pregnancy. However, different patients benefit distinctly from the surgery. Therefore, the study aims at examining the possible factors of spontaneous pregnancy after laparoscopic varicocelectomy in infertile men.
Wenbiao Ren, Jiandong Qu, Bichen Xue, Jiao Hu, Xiongbing Zu

1488 related Products with: Infertility duration and preoperative sperm progressive motility are significant factors of spontaneous pregnancy after varicocele repair.

1000 tests100ug250ul10 mg100ug1 ml10 mg0.25mg5 g10 mg100ug2.5 mg

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#32770829   2020/08/08 To Up

Clinicopathological characteristics of lung cancer in patients with systemic sclerosis.

Systemic sclerosis (SSc) is a connective tissue disorder (CTD) associated with an increased risk of malignancy including lung cancer (LC). Our objective was to provide a description of demographics and clinicopathological characteristics of LC patients with SSc.
Minjiang Chen, Xiaoyan Liu, Yan Xu, Qing Zhou, Yuequan Shi, Dongming Zhang, Hongge Liang, Jing Zhao, Wei Zhong, Mengzhao Wang

1079 related Products with: Clinicopathological characteristics of lung cancer in patients with systemic sclerosis.



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#32770826   2020/08/08 To Up

Toxoplasmagondii oocysts, Giardia cysts and Cryptosporidium oocysts in outdoor swimming pools in Brazil.

The accidental ingestion of treated recreational water is an important transmission route of waterborne protozoa worldwide. The present study aimed to provide the first evaluation of swimming pools in Brazil, analysing the presence of pathogenic protozoa (Toxoplasma gondii, Cryptosporidium spp. and Giardia spp.) by parasitological and molecular methods. A total of 57 samples were collected from 21 public swimming pools, either directly from the pool or filter backwash water and concentrated using the membrane filtration technique. Giardia cysts and Cryptosporidium oocysts were visualized by direct immunofluorescence assay after purification by immunomagnetic separation. Toxoplasma gondii oocysts were detected by autofluorescence visualization using the supernatant discarded during the purification step as a sample. Positive samples were submitted to molecular analysis. The molecular markers were used: SSU-rRNA, tpi, gdh and bg, for Giardia DNA amplification, and 18S rRNA gene fragment amplification was used for the Cryptosporidium oocysts. The 529-bp repeat element (REP529) fragment and the 35-fold repetitive B1 gene were employed as a target for T. gondii. Amplified products were submitted to sequencing and phylogenetic analysis. Giardia cysts were detected in 19.0% and Cryptosporidium oocysts in 9.5% of swimming pools. In one swimming pool (4.7%), both protozoa were detected on at least one occasion. Structures similar to T. gondii oocysts were detected in 33.3% of the samples, ranging from one to 23 per slide. Giardia was confirmed by DNA amplification in three swimming pools; Giardia duodenalis Assemblage A was identified by the phylogenetic positioning of the β-giardin gene. Toxoplasma gondii DNA was detected in 14.2% of swimming pools. The present study represents the first report of the occurrence of T. gondii oocysts in swimming pools. Recreational activity in swimming pools contaminated by chlorine-resistant protozoa can represent a high risk of infection for bathers and swimmers.
Carolina Ortiz Pineda, Diego Averaldo Guiguet Leal, Vagner Ricardo da Silva Fiuza, Juliana Jose, Guilherme Borelli, Mauricio Durigan, Hilda Fátima Jesus Pena, Regina Maura Bueno Franco

2521 related Products with: Toxoplasmagondii oocysts, Giardia cysts and Cryptosporidium oocysts in outdoor swimming pools in Brazil.

1 mL100 100 assays96tests50 mg100ug100ug Lyophilized1 g1 Set

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#32770824   2020/08/08 To Up

Adenoid cystic carcinoma of the salivary gland metastasizing to the pericardium and diaphragm: Report of a rare case.

Adenoid cystic carcinoma (AdCC) is an uncommon malignancy of the salivary gland characterized by slow growth, increased risk of recurrence and poor prognosis. The annual incidence in the United States is approximately 1200 cases per year and rarely involves the body cavities.
Nadia Nasir, Cara E Monroe, Brendan L Hagerty, Martha M Quezado, Mark J Roth, David S Schrump, Armando C Filie, Tanupriya Agrawal

2470 related Products with: Adenoid cystic carcinoma of the salivary gland metastasizing to the pericardium and diaphragm: Report of a rare case.

100.00 ul1 ml 100ul100.00 ul1 ml100

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