Search results for: Human Tenascin C Elisa Kit (TNC)
#35053372 
2022/01/13
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Mechanical Compression of Human Airway Epithelial Cells Induces Release of Extracellular Vesicles Containing Tenascin C.
Aberrant remodeling of the asthmatic airway is not well understood but is thought to be attributable in part to mechanical compression of airway epithelial cells. Here, we examine compression-induced expression and secretion of the extracellular matrix protein tenascin C (TNC) from well-differentiated primary human bronchial epithelial (HBE) cells grown in an air-liquid interface culture. We measured mRNA expression using RT-qPCR and secreted TNC protein using Western blotting and ELISA. To determine intracellular signaling pathways, we used specific inhibitors for either ERK or TGF-β receptor, and to assess the release of extracellular vesicles (EVs) we used a commercially available kit and Western blotting. At baseline, secreted TNC protein was significantly higher in asthmatic compared to non-asthmatic cells. In response to mechanical compression, both mRNA expression and secreted TNC protein was significantly increased in both non-asthmatic and asthmatic cells. TNC production depended on both the ERK and TGF-β receptor pathways. Moreover, mechanically compressed HBE cells released EVs that contain TNC. These data reveal a novel mechanism by which mechanical compression, as is caused by bronchospasm, is sufficient to induce the production of ECM protein in the airway and potentially contribute to airway remodeling.Chimwemwe Mwase, Thien-Khoi N Phung, Michael J O'Sullivan, Jennifer A Mitchel, Margherita De Marzio, Ayşe Kılıç, Scott T Weiss, Jeffrey J Fredberg, Jin-Ah Park
1892 related Products with: Mechanical Compression of Human Airway Epithelial Cells Induces Release of Extracellular Vesicles Containing Tenascin C.
25 TESTS25 21.00 flask200 1.00 flask4 X 250 ml.25 1.00 flask1.00 flask2
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#20953929 2010/10/16
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Distribution and role of tenascin-C in human osteoarthritic cartilage.
Tenascin-C (TN-C) is expressed in the cartilage of osteoarthritis (OA). We examined whether TN-C was involved in cartilage repair of the diseased joints. Human articular cartilage samples were obtained from patients with OA and those with normal joints.Yutaka Nakoshi, Masahiro Hasegawa, Koji Akeda, Takahiro Iino, Akihiro Sudo, Toshimichi Yoshida, Atsumasa Uchida
2367 related Products with: Distribution and role of tenascin-C in human osteoarthritic cartilage.
100 UG100 μg4 Membranes/Box100 μg100 μg100 100 μg16 Arrays/Slide100 μg96 tests4 Membranes/Box
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#17322629 //
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Higher serum tenascin-C levels reflect the severity of heart failure, left ventricular dysfunction and remodeling in patients with dilated cardiomyopathy.
Tenascin-C (TN-C), an extracellular matrix glycoprotein, is specifically expressed at high levels during embryonic development, but not in the adult heart. TN-C reappears at sites of inflammatory tissue remodeling or wound healing under various pathologic conditions, such as acute myocardial infarction, acute myocarditis, and some cases of cardiomyopathy. Therefore, the expression of TN-C might be useful for detecting the clinical characteristics of, and ventricular remodeling in, dilated cardiomyopathy (DCM).Fumio Terasaki, Hiroshi Okamoto, Katsuya Onishi, Akira Sato, Hiroaki Shimomura, Bin Tsukada, Kyoko Imanaka-Yoshida, Michiaki Hiroe, Toshimichi Yoshida, Yasushi Kitaura, Akira Kitabatake,