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#33068696   2020/10/14 To Up

Comprehensive proteomic analysis revealing multifaceted regulatory network of the xero-halophyte Haloxylon salicornicum involved in salt tolerance.

Haloxylon salicornicum is a xero-halophyte which grow predominantly in dry saline areas. However, the proteomic approach for revealing the regulatory network involved in salt adaptation of this important xerohalophyte has not been studied so far. In the present investigation, the label-free quantitative proteomic analysis was carried out in shoot of H. salicornicum to get an insight into the functional network of proteins involved in salt tolerance. Comparative proteomic analysis in control and salt treated plants of H. salicornicum by nano-ESI-LC-MS and MS/MS, and data base searching led to the identification of 723 proteins. Pathway enrichment analysis by KEGG uncovered various biological pathways to which salinity induced differentially regulated proteins are involved. In H. salicornicum, out of 723 identified proteins, 188 proteins were differentially regulated in response to salinity. In addition to significant up-regulation of stress responsive proteins, other proteins involved in carbohydrate metabolism, TCA cycle, protein synthesis, antioxidative defense systems, energy transfer, ion transport, nucleotide binding, and proteosomal proteins also significantly up-regulated under salinity in H. salicornicum. The major photosynthetic proteins up-regulated were RuBisCo, D1 protein, photosystem II-CP47, and cytochrome b599. TCA cycle component proteins such as citrate synthase, succinate dehydrogenase, and malate dehydrogenase upregulated indicating their significant roles in providing vital energy for salinity tolerance. Salinity induced higher expressions of ion transporters in H. salicornicum suggest efficient compartmentalization of toxic sodium ions. In addition, up-regulation of antioxidative defense system can be correlated with effective scavenging of salinity induced ROS and hence imparting salt tolerance. In H. salicornicum, protein synthesis was boosted under salinity as confirmed from the salinity-induced up-regulation of the ribosome associated proteins. Salinity induced significantly changed proteins of the ribosomal pathway include ribosomal protein components such as elongation factor-Tu (EF-Tu), initiation factor 1 and 2 (IF1, 2), Rpo cluster C and B, etc. Functional integrity of protein synthesis machinery in H. salicornicum is maintained under high salinity by higher abundance of ribosomal subunit proteins in NaCl-treated plants. We assume that consistent energy supply by the up-regulations TCA cycle along with uninterrupted protein synthesis and maintenance of structural integrity of the photosynthetic machinery are the primary mechanism of salinity tolerance of H. salicornicum. In the present study, we comprehensively elucidated possible mechanisms associated with systematic salt tolerance of H. salicornicumemploying proteomic approach. The information from this study will contribute to thegenetic improvement of crop plants that can be grown in saline marginal lands.
Ashok Panda, Jaykumar Rangani, Asish Kumar Parida

2319 related Products with: Comprehensive proteomic analysis revealing multifaceted regulatory network of the xero-halophyte Haloxylon salicornicum involved in salt tolerance.

50 UG11 mg96 wells1 g100

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#33065099   2020/10/13 To Up

Inhibition of the mitochondrial ATPase function by IF1 changes the spatiotemporal organization of ATP synthase.


Verena Weissert, Bettina Rieger, Silke Morris, Tasnim Arroum, Olympia Ekaterini Psathaki, Thomas Zobel, Guy Perkins, Karin B Busch

2948 related Products with: Inhibition of the mitochondrial ATPase function by IF1 changes the spatiotemporal organization of ATP synthase.

100 ul100ul100ug1mg100 IU100ug Lyophilized0.1 mg100ug Lyophilized1mg 100ul 100 G

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#33056988   2020/10/14 To Up

Analysis of translating mitoribosome reveals functional characteristics of translation in mitochondria of fungi.

Mitoribosomes are specialized protein synthesis machineries in mitochondria. However, how mRNA binds to its dedicated channel, and tRNA moves as the mitoribosomal subunit rotate with respect to each other is not understood. We report models of the translating fungal mitoribosome with mRNA, tRNA and nascent polypeptide, as well as an assembly intermediate. Nicotinamide adenine dinucleotide (NAD) is found in the central protuberance of the large subunit, and the ATPase inhibitory factor 1 (IF) in the small subunit. The models of the active mitoribosome explain how mRNA binds through a dedicated protein platform on the small subunit, tRNA is translocated with the help of the protein mL108, bridging it with L1 stalk on the large subunit, and nascent polypeptide paths through a newly shaped exit tunnel involving a series of structural rearrangements. An assembly intermediate is modeled with the maturation factor Atp25, providing insight into the biogenesis of the mitoribosomal large subunit and translation regulation.
Yuzuru Itoh, Andreas Naschberger, Narges Mortezaei, Johannes M Herrmann, Alexey Amunts

2191 related Products with: Analysis of translating mitoribosome reveals functional characteristics of translation in mitochondria of fungi.

5 G1 Set100 2ug2ug1 kit(96 Wells)2 Pieces/Box50μg1 mg100 μg

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#33037618   2020/10/10 To Up

NHS: a novel pharmacological inhibitor of the mitochondrial F F -ATPase which represses viability of cancerous cells.

The mitochondrial enzyme F F -ATPsynthase is core to cellular homeostasis. Its function is compromised in acute pathologies such as ischemia, as well as in those caused by long-term acquired metabolic dysfunctions. This makes the F F -ATPsynthase an important target for therapeutic interventions in diseases such as cancer. Despite this, pharmacological tools to selectively inhibit the hydrolysis of ATP by the F Fo-ATPase without affecting its synthesis remain scarce. Here, we report the synthesis and in vitro characterization of the NH-Sulfoximine (NHS) that is the suxolfimine analogue of the compound BTB-06584 (BTB) which we characterized as F Fo-ATPase inhibitor.
Daniela Strobbe, Rosalba Pecorari, Oriana Conte, Antonella Minutolo, Christine M M Hendriks, Stefan Wiezorek, Danilo Faccenda, Rosella Abeti, Carla Montesano, Carsten Bolm, Michelangelo Campanella

2844 related Products with: NHS: a novel pharmacological inhibitor of the mitochondrial F F -ATPase which represses viability of cancerous cells.

0.1 mg 1 kit(s) 100ug Lyophilized1 kit20 100ug Lyophilized1.00 flask25 1.00 flask

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#33032063   2020/09/22 To Up

Identification and characterization of Arabidopsis thaliana mitochondrial FF-ATPase inhibitor factor 1.

Mitochondrial FF-ATP synthase (FF-ATPase) inhibitor factor 1 (IF1) has been extensively characterized as an endogenous inhibitor that prevents the hydrolysis of adenosine-5'-triphosphate (ATP) by mitochondrial ATPases in mammals and yeasts; however, IF1's functions in plants remain unclear. Here, a comprehensive bioinformatic analysis was performed to identify plant mitochondrial FF-ATPase IF1 orthologs. Plant IF1s contain a conserved FF-ATPase inhibitory domain, but lack the antiparallel α-helical coiled-coil structure compared with mammalian IF1s. A subcellular localization analysis in Arabidopsis thaliana revealed that AtIF1-green fluorescent protein was present only in mitochondria. Additionally, AtIF1 was widely expressed in diverse organs and intense β-glucuronidase staining was observed in reproductive tissues and germinating seeds. Compared with the wild-type and p35S:AtIF1-if1 etiolated seedlings, the ATP/ADP ratio was significantly lower in the AtIF1 T-DNA knockout seedlings (if1 mutant) growing under dark conditions, suggesting that AtIF1 can influence the energy state of cells. A significant reduction in seed yield and strong growth retardation under dark conditions were observed in the if1 mutant line. Furthermore, if1 plants exhibited a substantially decreased sensitivity to abscisic acid. Thus, the A. thaliana mitochondrial IF1, which is a conserved FF-ATPase inhibitor, is crucial for plant growth and responses to abscisic acid.
Cuiting Chen, Yiqing Meng, Jannat Shopan, James Whelan, Zhongyuan Hu, Jinghua Yang, Mingfang Zhang

1975 related Products with: Identification and characterization of Arabidopsis thaliana mitochondrial FF-ATPase inhibitor factor 1.

100.00 ug10 5 2 5ug100 μg2ug1 g100ug Lyophilized0.1mg0.1 mg

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#32956760   2020/09/18 To Up

The ATPase Inhibitory Factor 1 (IF) regulates the expression of the mitochondrial Ca uniporter (MCU) via the AMPK/CREB pathway.


Danilo Faccenda, Giulia Gorini, Adam Jones, Claire Thornton, Alessandra Baracca, Giancarlo Solaini, Michelangelo Campanella

1381 related Products with: The ATPase Inhibitory Factor 1 (IF) regulates the expression of the mitochondrial Ca uniporter (MCU) via the AMPK/CREB pathway.

100ulmin 2 cartons500 Units1 ml1mg

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#32887042   2020/06/30 To Up

A reference measurement of circulating ATPase inhibitory factor 1 (IF1) in humans by LC-MS/MS: Comparison with conventional ELISA.

ATPase inhibitory factor 1 (IF1) is a 9.5 kDa protein that binds to mitochondrial and plasma membrane ATP synthase and selectively inhibits ATP hydrolysis. Recently, IF1 was identified in systemic circulation in humans. IF1 appeared as an independent determinant of HDL-cholesterol with lower levels in coronary heart disease (CHD) patients. Moreover, IF1 was also found to negatively associate with mortality in these patients, supporting the notion that circulating IF1 could be a promising biomarker of cardiovascular disease. However, in previous studies, IF1 was quantified by a non-standardized competitive enzyme-linked immunosorbent assay (ELISA). Herein, we have validated a liquid chromatography-tandem mass spectrometry method (LC-MS/MS) enabling the accurate quantification of IF1 in human plasma. Plasma IF1 was trypsin-digested through an optimized procedure before LC-MS/MS analysis. The method was successfully validated over 4 independent experiments into the range of 100-1500 ng/mL. Intra- and inter-assay variation coefficients had never exceeded 14.2% and accuracy ranged between 95% and 102% for the selected EAGGAFGK peptide marker. Subsequently, the results of the LC-MS/MS method were compared with those obtained using ELISA in 204 individuals from the GENES study. We found that IF1 plasma levels obtained using both techniques were strongly correlated (r = 0.89, p < 0.0001), while the Bland-Altman plot did not indicate any major statistically significant differences. To clinically validate LC-MS/MS, we confirmed the positive correlation between IF1 plasma levels and HDL-cholesterol (r = 0.38, p < 0.0001). Besides, we found lower IF1 plasma levels in CHD patients compared to controls (431 ± 132 ng/mL and 555 ± 173 ng/mL, respectively; p < 0.0001). Hence, it can be concluded that the presented LC-MS/MS analytical method provides a highly specific strategy for IF1 quantification in human plasma and could be proposed as a reference method.
Annelise Genoux, Thibaut Duparc, Jean-Bernard Ruidavets, Cécile Ingueneau, Souad Najib, Jean Ferrières, Bertrand Perret, Mikaël Croyal, Laurent O Martinez

2304 related Products with: A reference measurement of circulating ATPase inhibitory factor 1 (IF1) in humans by LC-MS/MS: Comparison with conventional ELISA.

100uL100ul100ug Lyophilized100ug Lyophilized100ul100ug Lyophilized96 tests 100ul100.00 ug100ug100ug100ug Lyophilized

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#32871222   2020/08/29 To Up

Comparative genomic analysis of eutherian interferon genes.

The eutherian interferons were implicated as paradigmatic effector proteins in innate and acquired immunity. Yet, familiar interferon classification and nomenclature disagreed with functional genomics, phylogenetic and evolutionary analyses. Using eutherian comparative genomic analysis protocol and 35 public eutherian reference genomic sequence data sets, the present analysis attempted to resolve major disagreements in descriptions of eutherian interferons. Among 836 eutherian interferon potential coding sequences, the tests of reliability of eutherian public genomic sequences annotated, in aggregate, 495 complete coding sequences that were partitioned into supercluster IF1 and supercluster IF2 gene data sets. There were 29 human class 2 cytokine genes described, including initially described human IF1IC2 gene. There was convergence between nonhomologous eutherian supercluster IF1 and supercluster IF2 protein primary structures including common cysteine amino acid residues implicated in disulfide bonding. The integrated gene annotations, phylogenetic analysis and protein molecular analysis proposed revised and updated classification and nomenclature of eutherian interferons.
Marko Premzl

2360 related Products with: Comparative genomic analysis of eutherian interferon genes.

100 extractions 15 ml 1 module100.00 ug100 2 modules2 modules1 module200ul1 module1 moduleProteinase K Powder, 2 ea

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#32769215   // To Up

The role of mitochondrial ATP synthase in cancer.

The mitochondrial ATP synthase is a multi-subunit enzyme complex located in the inner mitochondrial membrane which is essential for oxidative phosphorylation under physiological conditions. In this review, we analyse the enzyme functions involved in cancer progression by dissecting specific conditions in which ATP synthase contributes to cancer development or metastasis. Moreover, we propose the role of ATP synthase in the formation of the permeability transition pore (PTP) as an additional mechanism which controls tumour cell death. We further describe transcriptional and translational modifications of the enzyme subunits and of the inhibitor protein IF1 that may promote adaptations leading to cancer metabolism. Finally, we outline ATP synthase gene mutations and epigenetic modifications associated with cancer development or drug resistance, with the aim of highlighting this enzyme complex as a potential novel target for future anti-cancer therapy.
Chiara Galber, Manuel Jesus Acosta, Giovanni Minervini, Valentina Giorgio

2674 related Products with: The role of mitochondrial ATP synthase in cancer.



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#32752195   2020/08/01 To Up

Quantification of the Effect of the Cattle Breed on Milk Cheese Yield: Comparison between Italian Brown Swiss and Italian Friesian.

Milk from different cattle breeds can present different casein and fat contents, which are reflected in different cheese yields (CY). However, CY is also related to some breed-related molecular characteristics. The aim of the present work was to quantify the effect of these characteristics by comparing a series of Parmigiano Reggiano (PR) cheese-making trials made with milks from Italian Brown (IB) and Italian Friesian (IF) cattle herds. Twelve trials were carried out in a cheese factory in one year (one trial per month), each one consisting of four vats processed in parallel: three vats contained milk from three different IF cattle herds (IF1, IF2 and IF3) and one contained milk from a single IB cattle herd. A 24-h CY prediction formula was developed with data from IF1, IF2 and IF3 trials (calibration) and successively validated by applying it to 12 PR trials made with IF milk in six different cheese factories (external validation). The predicted values of 24-h CY were no different to the actual ones in both calibration and external validation. Finally, the formula was tested on trials made with IB milk. In this case, the predicted values were lower than the actual ones. The quantity of IF milk casein necessary to give the same CY of IB milk was 0.20 g/100 g.
Piero Franceschi, Massimo Malacarne, Paolo Formaggioni, Michele Faccia, Andrea Summer

1013 related Products with: Quantification of the Effect of the Cattle Breed on Milk Cheese Yield: Comparison between Italian Brown Swiss and Italian Friesian.

50 IU100.00 ul500 Units1 100ul100 U100.00 ul

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