Search results for: IHC
#33080410 2020/10/14 To Up
The involvement of hsa_circ_0000417 in the development of hypospadias by regulating AR.Congenital hypospadias is a common congenital malformation of the urinary system in male children. However, the role of circRNA in congenital hypospadias remains unknown.
Chao Chen, Yong-Yuan Jian, Xiang-You Zhao, Yu-Ling Liu, Qi-Ke Xie
1941 related Products with: The involvement of hsa_circ_0000417 in the development of hypospadias by regulating AR.1300 units100 U
#33080107 2020/10/20 To Up
TGFBI modulates tumour hypoxia and promotes breast cancer metastasis.Breast cancer metastasis is a complex process that depends on intrinsic characteristics of metastatic stem cells, but also on the particular microenvironment that supports their growth and modulates the plasticity of the system. In search for microenvironmental factors supporting cancer stem cell (CSC) growth and tumour progression to metastasis, we here investigated the role of the matricellular protein Transforming Growth Factor Beta Induced (TGFBI) in breast cancer. We crossed the MMTV-PyMT model of mammary gland tumourigenesis with a Tgfbi mouse, and studied the CSC content of the tumours. We performed RNAseq on wt and ko tumours, and analysed the tumour vasculature and the immune compartment by IHC and FACS. The source of TGFBI expression was determined by qPCR and by bone marrow transplantation experiments. Finally, we performed in silico analyses using the METABRIC cohort to assess the potential prognostic value of TGFBI. We observed that deletion of Tgfbi led to a dramatic decrease in CSC content and lung metastasis. Our results show that lack of TGFBI resulted in tumour vessel normalisation, with improved vessel perfusion and decreased hypoxia, a major factor controlling CSCs and metastasis. Furthermore, human data mining in a cohort of breast cancer patients showed that higher expression of TGFBI correlates with poor prognosis and is associated with the more aggressive subtypes of breast cancer. Overall, these data reveal a novel biological mechanism controlling metastasis that could potentially be exploited to improve the efficacy and delivery of chemotherapeutic agents in breast cancer.
Flavia Fico, Albert Santamaria-Martínez
#33080078 2020/10/20 To Up
The protective role of corilagin on renal calcium oxalate crystal-induced oxidative stress, inflammatory response, and apoptosis via PPAR-γ and PI3K/Akt pathway in rats.Kidney stones, also known as calcium oxalate nephrolithiasis, are often asymptomatic, leading to kidney injury and renal failure complications. Corilagin, a gallotannin found in various plants and known to elicit various biological activities. The present study aimed to elucidate the renoprotective effect of corilagin against the rats' renal stones deposition. The rats were induced for nephrolithiasis (calcium oxalate (CaOx) deposition) using 0.75% ethylene glycol (EG) in their drinking water. Then they were treated with corilagin at 50 mg/kg/day and 100 mg/kg/day for 4 weeks. At the end of the experimental period, the rats were killed; blood and renal tissues were collected for various histological, biochemical, and gene expression analyses. The results demonstrated that the rats had renal calculi displayed a significant increase in serum creatinine (59.39μmol/L) and BUN (19.03mmol/L) levels compared to control. Moreover, the malondialdehyde (MDA) (13.29nmol/mg) level was found to increase with a profound reduction in antioxidants' activities with up-regulated inflammatory cytokines. In contrast, the RT-PCR and immunohistochemistry (IHC) analysis demonstrated a substantial reduction in cell survival markers PPAR-γ and PI3K/Akt with an apparent increase in apoptosis markers genes expressions in rats suffering from renal stones. Thus, the present study results suggest that corilagin could suppress renal CaOx crystal-induced oxidative stress, inflammatory response, and apoptosis via PPAR-γ and PI3K/Akt mediated pathway. This article is protected by copyright. All rights reserved.
Haibo Yuan, Jinghong Zhang, Xiaosong Yin, Tongwei Liu, Xiao Yue, Chuangui Li, Yuanyuan Wang, Ding Li, Qiang Wang
2018 related Products with: The protective role of corilagin on renal calcium oxalate crystal-induced oxidative stress, inflammatory response, and apoptosis via PPAR-γ and PI3K/Akt pathway in rats.100 UG2 Pieces/Box2 Pieces/Box1 mg2 Pieces/Box7 inhibitors5mg100ug1 Set200ug
#33080075 2020/10/20 To Up
Linking epigenetic signature and metabolic phenotype in IDH mutant and IDH wildtype diffuse glioma.Changes in metabolism are known to contribute to tumour phenotypes. If and how metabolic alterations in brain tumours contribute to patient outcome is still poorly understood. Epigenetics impact metabolism and mitochondrial function. The aim of this study is a characterization of metabolic features in molecular subgroups of isocitrate dehydrogenase mutant (IDHmut) and isocitrate dehydrogenase wildtype (IDHwt) gliomas.
Yannick Braun, Katharina Filipski, Simon Bernatz, Peter Baumgarten, Bastian Roller, Jenny Zinke, Pia S Zeiner, Elena Ilina, Christian Senft, Michael W Ronellenfitsch, Karl H Plate, Oliver Bähr, Elke Hattingen, Joachim P Steinbach, Michel Mittelbronn, Patrick N Harter
2450 related Products with: Linking epigenetic signature and metabolic phenotype in IDH mutant and IDH wildtype diffuse glioma.5 G 5 G100ul25 mg50 ug 96 wells (1 kit)500 gm.1 g10 mg1000 TESTS/0.65ml100 mg100ug
#33078654 2020/10/20 To Up
and reciprocally regulate expression and autophagy in nonalcoholic hepatic steatosis.Macroautophagy/autophagy, a self-degradative process, regulates metabolic homeostasis in response to various stress conditions and is a therapeutic target for nonalcoholic fatty liver disease. We found that autophagic activity was inhibited as a result of a significant reduction in the expression of autophagy-related genes such as in a mouse model and patients with fatty liver. This downregulation was caused by increased levels and decreased mRNA levels in hepatocytes. suppressed expression through direct binding at a 3' untranslated region sequence. directly activated transcription of . We investigated lipid accumulation and the expression of autophagy-related genes in the livers of mice treated with anti-. Hepatic steatosis was alleviated, and mRNA levels were significantly increased by locked nucleic acid-mediated silencing. Additionally, autophagosome formation and MAP1LC3/LC3-II protein levels were increased, indicating an increase in autophagic activity. Interestingly, suppression of did not ameliorate fatty liver under suppression, suggesting that reduced levels mitigate hepatic steatosis through upregulation of . These results demonstrate that inhibition of expression ameliorated fatty liver disease through increased autophagic activity by increasing the expression of . Thus, is a potential therapeutic target for nonalcoholic fatty disease. : AMPK: adenosine monophosphate-activated protein kinase; ATG: autophagy-related; ChIP: chromatin immunoprecipitation; CTSB: cathepsin B; CTSL: cathepsin L; CQ: chloroquine; HFD: high-fat diet; HNF4A: hepatocyte nuclear factor 4, alpha; IF: immunofluorescence; IHC: immunohistochemistry; LDs: lipid droplets; Leup: leupeptin; LFD: low-fat diet; LNA: locked nucleic acid; MAP1LC3B/LC3B: microtubule-associated protein 1 light chain 3 beta; miRNA: microRNA; MTOR: mechanistic target of rapamycin kinase; NAFLD: non-alcoholic fatty liver disease; NASH: non-alcoholic steatohepatitis; PCR: polymerase chain reaction; TEM: transmission electron microscopy; TF: transcription factor; TLDA: TaqMan low-density array; ULK1: unc-51 like kinase 1; UTR: untranslated region.
Da-Hye Lee, So-Hyun Park, Jiyun Ahn, Seung Pyo Hong, Eunyoung Lee, Young-Jin Jang, Tae-Youl Ha, Yang Hoon Huh, Seung-Yeon Ha, Tae-Il Jeon, Chang Hwa Jung
1108 related Products with: and reciprocally regulate expression and autophagy in nonalcoholic hepatic steatosis.1000 tests100ug10 mg10 mg100ug500 mg1 ml25 mg 5 G 5 G100ul50 ug
#33077919 2020/10/19 To Up
Detection of ERBB2 amplification in uterine serous carcinoma by next-generation sequencing: an approach highly concordant with standard assays.Uterine serous carcinoma is an aggressive subtype of endometrial cancer that accounts for fewer than 10% of endometrial carcinomas but is responsible for about half of deaths. A subset of cases has HER2 overexpression secondary to ERBB2 gene amplification, and these patients may benefit from anti-HER2 therapies, such as trastuzumab. HER2 protein overexpression is currently assessed by immunohistochemistry (IHC) and ERBB2 gene amplification by fluorescence in situ hybridization (FISH). Targeted next-generation sequencing (NGS) is increasingly used to routinely identify predictive and prognostic molecular abnormalities in endometrial carcinoma. To investigate the ability of a targeted NGS panel to detect ERBB2 amplification, we identified cases of uterine serous carcinoma (n = 93) and compared HER2 expression by IHC and copy number assessed by FISH with copy number status assessed by NGS. ERBB2 copy number status using a combination of IHC and FISH was interpreted using the 2018 ASCO/CAP guidelines for breast carcinoma. ERBB2 amplification by NGS was determined by the relative number of reads mapping to ERBB2 in tumor DNA compared to control nonneoplastic DNA. Cases with copy number ≥6 were considered amplified and copy number <6 were non-amplified. By IHC, 70 specimens were classified as negative (0 or 1+), 19 were classified as equivocal (2+), and 4 were classified as positive (3+). Using combined IHC/FISH, ERBB2 amplification was observed in 8 of 93 cases (9%). NGS identified the same 8 cases with copy number ≥6; all 85 others had copy number <6. In this series, NGS had 100% concordance with combined IHC/FISH in identifying ERBB2 amplification. NGS is highly accurate in detecting ERBB2 amplification in uterine serous carcinoma and provides an alternative to measurement by IHC and FISH.
Carrie L Robinson, Beth T Harrison, Azra H Ligon, Fei Dong, Valeria Maffeis, Ursula Matulonis, Marisa R Nucci, David L Kolin
2888 related Products with: Detection of ERBB2 amplification in uterine serous carcinoma by next-generation sequencing: an approach highly concordant with standard assays.4 Membranes/Box4 Arrays/Slide1 Set4 Arrays/Slide2 Pieces/Box4 Membranes/Box2 Pieces/Box4 Arrays/Slide4 Membranes/Box2 Pieces/Box
#33077794 2020/10/19 To Up
3D histopathology of human tumours by fast clearing and ultramicroscopy.Here, we describe a novel approach that allows pathologists to three-dimensionally analyse malignant tissues, including the tumour-host tissue interface. Our visualization technique utilizes a combination of ultrafast chemical tissue clearing and light-sheet microscopy to obtain virtual slices and 3D reconstructions of up to multiple centimetre sized tumour resectates. For the clearing of tumours we propose a preparation technique comprising three steps: (a) Fixation and enhancement of tissue autofluorescence with formalin/5-sulfosalicylic acid. (b) Ultrafast active chemical dehydration with 2,2-dimethoxypropane and (c) refractive index matching with dibenzyl ether at up to 56 °C. After clearing, the tumour resectates are imaged. The images are computationally post-processed for contrast enhancement and artefact removal and then 3D reconstructed. Importantly, the sequence a-c is fully reversible, allowing the morphological correlation of one and the same histological structures, once visualized with our novel technique and once visualized by standard H&E- and IHC-staining. After reverting the clearing procedure followed by standard H&E processing, the hallmarks of ductal carcinoma in situ (DCIS) found in the cleared samples could be successfully correlated with the corresponding structures present in H&E and IHC staining. Since the imaging of several thousands of optical sections is a fast process, it is possible to analyse a larger part of the tumour than by mechanical slicing. As this also adds further information about the 3D structure of malignancies, we expect that our technology will become a valuable addition for histological diagnosis in clinical pathology.
Inna Sabdyusheva Litschauer, Klaus Becker, Saiedeh Saghafi, Simone Ballke, Christine Bollwein, Meraaj Foroughipour, Julia Gaugeler, Massih Foroughipour, Viktória Schavelová, Viktória László, Balazs Döme, Christine Brostjan, Wilko Weichert, Hans-Ulrich Dodt
2157 related Products with: 3D histopathology of human tumours by fast clearing and ultramicroscopy.20 100ul1 ml1000 5 100ug Lyophilized 100ul100ug Lyophilized100ug Lyophilized 100ul1 mg
#33077383 2020/10/12 To Up
Fatty acid ethyl ester (FAEE) associated acute pancreatitis: An ex-vivo study using human pancreatic acini.Fatty acid ethyl esters (FAEEs), are produced by non-oxidative alcohol metabolism and can cause acinar cell damage and subsequent acute pancreatitis in rodent models. Even though experimental studies have elucidated the FAEE mediated early intra-acinar events, these mechanisms have not been well studied in humans. In the present study, we evaluate the early intra-acinar events and inflammatory response in human pancreatic acinar tissues and cells in an ex-vivo model.
Aparna Jakkampudi, Ramaiah Jangala, Ratnakar Reddy, Balkumar Reddy, G Venkat Rao, Rebala Pradeep, D Nageshwar Reddy, Rupjyoti Talukdar
2213 related Products with: Fatty acid ethyl ester (FAEE) associated acute pancreatitis: An ex-vivo study using human pancreatic acini.50ul 100ul 100ul100 mg 1 G25 mg100 mg100μg10 mg100 mg10 mg25 mg
#33075373 2020/10/16 To Up
STX2 drives colorectal cancer proliferation via upregulation of EXOSC4.To explore the biological function and mechanism of Syntaxin2 (STX2) in Colorectal cancer (CRC) proliferation.
Yong-Xia Wang, Yong-Zhen Li, Hui-Fang Zhu, Zhe-Ying Zhang, Xin-Lai Qian, Guo-Yang HeEach
#33075198 2020/10/19 To Up
Intraoperative diagnosis of lymph node metastasis during segmentectomy for non-small cell lung cancer by rapid immunohistochemistry using noncontact alternating current electric field mixing.Although lobectomy is considered the standard surgery for any non-small cell lung cancer (NSCLC), recent evidence indicates that for early NSCLCs segmentectomy may be equally effective. For segmentectomy to be oncologically safe, however, adequate intraoperative lymph node staging is essential. The aim of this study was to compare the results of a new rapid-IHC system to the HE analysis for intraoperative nodal diagnosis in lung cancer patients considered for segmentectomy.
Kazuhiro Imai, Hiroshi Nanjo, Shinogu Takashima, Yuko Hiroshima, Maiko Atari, Tsubasa Matsuo, Shoji Kuriyama, Yoshiaki Ishii, Yuki Wakamatsu, Yusuke Sato, Satoru Motoyama, Hajime Saito, Kyoko Nomura, Yoshihiro Minamiya
1247 related Products with: Intraoperative diagnosis of lymph node metastasis during segmentectomy for non-small cell lung cancer by rapid immunohistochemistry using noncontact alternating current electric field mixing.
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