Search results for: IHC
#32745914 2020/07/31 To Up
Radix Rehmanniae Praeparata promotes bone fracture healing through activation of TGF-β signaling in mesenchymal progenitors.Radix Rehmanniae Praeparata (RR), the steamed roots of Rehmannia glutinosa, is a traditional Chinese medicine with the function of kidney-nourishing, and it has been safety used for centuries to treat bone-related disorders. The aim of this study is to investigate the positive effect and underlying mechanism of RR enhancing bone fracture healing in mouse model.
Rui Xu, Cheng Luo, Qinwen Ge, Jun Ying, Peng Zhang, Chenjie Xia, Liang Fang, Huihui Xu, Wenhua Yuan, Taotao Xu, Shuaijie Lv, Hongting Jin, Peijian Tong, Kun Tian, Pinger Wang
1589 related Products with: Radix Rehmanniae Praeparata promotes bone fracture healing through activation of TGF-β signaling in mesenchymal progenitors.2 Pieces/Box11 inhibitors100 μg2 Pieces/Box100ug2 Pieces/BoxInhibitors25 Bags/Unit
#32743007 2020/07/27 To Up
Transbronchial cryobiopsy: an effective tool in the diagnosis of lymphoproliferative disorders of the lung.Malignant lymphoproliferative disorders are rarely observed in the lung and, considering their clinical and radiological heterogeneity, diagnosis is often difficult and may require invasive methods. Transbronchial cryobiopsy has been confirmed as a new tool in the diagnosis of interstitial lung diseases, given its fewer risks and costs compared to surgical approach. This study is aimed at assessing the effectiveness of cryobiopsy in the diagnosis of lymphoproliferative disorders.
Rita Bianchi, Alessandra Dubini, Silvia Asioli, Claudia Ravaglia, Sara Tomassetti, Silvia Puglisi, Sara Piciucchi, Christian Gurioli, Carlo Gurioli, Roberto Fiocca, Venerino Poletti
2149 related Products with: Transbronchial cryobiopsy: an effective tool in the diagnosis of lymphoproliferative disorders of the lung.100.00 ul
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#32742470 2020/07/06 To Up
Prognostic implication and functional annotations of APOBEC3G expression in patients with Melanoma.Skin cutaneous melanoma (SKCM) is one of the most life-threatening malignancies damaging human health. APOBEC3G (A3G) has been found in several cancers; however, the role of A3G in SKCM is rarely studied. This study aimed to investigate the expression of A3G in tumor tissue and its prognostic value in SKCM patients. A total of 512 SKCM patients from the First Affiliated Hospital of Soochow University (FAHSU) and the Cancer Genome Atlas (TCGA) database were consecutively recruited in analyses. Differential transcriptional and proteome expression profiles were obtained from multiple datasets. GEPIA was used to assess the survival analysis between distinguished groups. Both univariate and multivariate Cox regression analysis was performed to address the influence of independent factors on disease-free survival (RFS) and overall survival (OS). In addition, 31 SKCM and 31 nevus tissues were collected for immunohistochemical (IHC) staining and evaluation. STRING, DAVID and Gene Set Enrichment Analysis (GSEA) was utilized to conduct a network of related genes and significant pathways. Furthermore, we investigated the relationship of A3G with tumor-infiltrating immune cells (TIICs) by TIMER and TISIDB. We found both transcriptional and proteomics expressions of A3G were elevated in SKCM. Survival analysis and ROC curves showed significant diagnostic and prognostic ability of A3G. IHC results showed increased expression of A3G in SKCM compared to nevus tissues. Importantly, A3G expression was closely associated with the immune-infiltrating levels of B cells, CD4+ T, CD8+ T, neutrophils, macrophages and dendritic cells. In summary, our study first reveals that elevated A3G expression is significantly correlated with better prognosis in SKCM patients. The role of A3G in SKCM demonstrated that it might be a prognostic and immunotherapeutic biomarker for SKCM.
Wei Han, Jun Xu, Guo-Liang Shen
1638 related Products with: Prognostic implication and functional annotations of APOBEC3G expression in patients with Melanoma.
#32742466 2020/07/06 To Up
The Prognostic Value of the Chromobox Family in Human Ovarian Cancer.Ovarian cancer is one of the most lethal gynecologic tumors in women and has a poor prognosis. The purpose of our study was to identify new prognostic markers in ovarian cancer. We examined the prognostic roles of mRNA expression of the chromobox (CBX) family in patients with ovarian cancer utilizing the Kaplan-Meier plotter database. The prognostic values and expression levels of CBX members associated with prognosis were further evaluated using KM plotter in diverse subgroups and immunohistochemistry (IHC) analysis in ovarian carcinoma. The results revealed that elevated CBX1-3 mRNA expression may predict poor overall survival (OS) and progression-free survival (PFS) outcomes in patients with ovarian cancer. Notably, in women with ovarian cancer, increased CBX1 mRNA expression was linked to a short OS in all stages and in the grade II and grade III subgroups. Additionally, CBX2 and CBX3 were strongly related to short OS in stage III+IV patients, and a link between high CBX3 mRNA expression and unfavorable OS in grade II patients was observed. High expression levels of CBX1 and CBX3 were significantly associated with chemotherapy resistance in ovarian cancer patients. IHC staining showed that the CBX1-3 proteins were upregulated in serous ovarian carcinoma tissues compared with normal ovarian tissues. Therefore, our results indicated that CBX1-3 could be attractive biomarkers for predicting poor prognosis of ovarian cancer.
Yichi Xu, Shuya Pan, Yizuo Song, Chunyu Pan, Cheng Chen, Xueqiong Zhu10
#32742181 2020/06/01 To Up
Effect of different treatments on macrophage differentiation in chronic obstructive pulmonary disease and repeated pulmonary infection.To observe the differentiation of macrophages in lung tissue and alveolar lavage fluid of mice with severe pulmonary infection and the changes after intervention with ceftriaxone and ulinastatin, and to explore the pathogenesis of severe pulmonary infection under immunosuppressive state and the intervention effect of two drugs.
Run Liu, Zhidian Wu, Hang Yu
1805 related Products with: Effect of different treatments on macrophage differentiation in chronic obstructive pulmonary disease and repeated pulmonary infection.96 tests10 ug1 mg96 tests 50 UG100 μg500 tests5ug100ug Lyophilized96 tests
#32741804 2020/07/24 To Up
Predictions of the dysregulated competing endogenous RNA signature involved in the progression of human lung adenocarcinoma.Lung adenocarcinoma (LUAD) is the most common histological subtype of lung cancer worldwide. Until now, the molecular mechanisms underlying LUAD progression have not been fully explained. This study aimed to construct a competing endogenous RNA (ceRNA) network to predict the progression in LUAD.
Dan Yang, Yang He, Bo Wu, Ruxi Liu, Nan Wang, Tieting Wang, Yannan Luo, Yunda Li, Yang Liu
2468 related Products with: Predictions of the dysregulated competing endogenous RNA signature involved in the progression of human lung adenocarcinoma.11mg10
#32741023 2020/08/02 To Up
Expression of palladin is associated with disease progression in metastatic high-grade serous carcinoma.To analyze the expression and clinical role of the actin-associated molecule palladin in serous effusions.
Ben Davidson, Annika Jøntvedt Bock, Arild Holth, Dag Andre Nymoen
2715 related Products with: Expression of palladin is associated with disease progression in metastatic high-grade serous carcinoma.
#32741000 2020/08/02 To Up
Two fatty acid synthase genes from the integument contribute to cuticular hydrocarbon biosynthesis and cuticle permeability in Locusta migratoria.Lipids of the insect cuticle have important roles in resistance against the arid environment and invasion of foreign substances. Fatty acid synthase (FAS) is an important enzyme of the insect lipid synthesis pathway. In the present study, we identified three FAS genes from transcriptome data of the migratory locust, Locusta migratoria, based on bioinformatics analyses. Among them, two FAS genes (LmFAS1 and LmFAS3) are highly expressed in the integument of fifth instar nymphs. Suppression of LmFAS1 and LmFAS3 by RNA interference caused lethality during ecdysis or shortly after molting. The weight of the locusts and the content of lipid droplets were reduced compared with those of the control. The results of gas chromatography-mass spectrometry analysis showed that knockdown of LmFAS3 led to a decrease of both cuticular hydrocarbons and inner hydrocarbons (CHCs and IHCs) contents, especially the content of methyl branched hydrocarbons. By contrast, knockdown of LmFAS1 only resulted in a decrease in the IHC content, but not that of CHCs. By consequence, in LmFAS1- and LmFAS3-suppressed locusts, hydrocarbon deficiency reduced desiccation resistance and enhanced cuticle permeability and sensitivity to insecticides. These results indicate that LmFAS1 and LmFAS3 are essential for hydrocarbon production and cuticle permeability, which play influential roles in waterproofing the insect cuticle. This article is protected by copyright. All rights reserved.
Yang Yang, Xiaoming Zhao, Niu Niu, Yiyan Zhao, Weimin Liu, Bernard Moussian, Jianzhen Zhang
2808 related Products with: Two fatty acid synthase genes from the integument contribute to cuticular hydrocarbon biosynthesis and cuticle permeability in Locusta migratoria.50ul96tests 100ul 100ul25 25 G96 tests100ug Lyophilized
#32738749 2020/07/23 To Up
Chronic retinal injury induced by white LED light with different correlated color temperatures as determined by microarray analyses of genome-wide expression patterns in mice.Widely used white light-emitting diodes (LEDs) currently deliver higher levels of blue light than conventional domestic light sources. The high intensity of the blue component is the main source of concern regarding possible health risks of LED to chronic light toxicity to the retina. Therefore, we analyzed retinal injury and genome-wide changes in gene expression induced by white LED light with different correlated color temperatures (CCTs) in a mouse model. Balb/c mice (10 weeks old) were exposed to LED light with CCTs of 2954, 5624, and 7378 K, at different illuminance levels (250, 500, 1000, and 3000 lx) and for different exposure times (7, 14, and 28 days). Hematoxylin and eosin staining revealed that exposure to 7378 K light at 250 lx for 28 days resulted in a significant reduction of outer nuclear layer (ONL) nuclei, whereas 2954 K light at <3000 lx led to only a mild reduction in the number of ONL nuclei. In addition, 5624 and 7378 K light at 3000 lx resulted in a significant increase in TUNEL-positive apoptotic nuclei, which was not found at an illuminance of 1000 lx. Genome-wide expression analyses showed that, compared to a control group, there were 121 upregulated differentially expressed genes (DEGs) and 458 downregulated DEGs found in the 7378 K group, and 59 upregulated and only 4 downregulated DEGs in the 2954 K group. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses showed that the DEGs were involved in 341 GO terms and 16 related pathways for the 7378 K group and in 12 GO terms and 7 related pathways for the 2954 K group. Signal pathways related to ubiquitin potentially played an important role in light-induced retinal degeneration. Furthermore, retinal immunohistochemistry (IHC) indicated downregulation of ubiquitin and autophagy function caused by 7378 K light. Taken together, these results indicate that retinal injury in the mice induced by white LED light occurred in a CCT-dependent manner, and that light with a higher CCT was more likely to reduce ONL nuclei; however, the apoptosis pathway may not be the only mechanism involved. Based on genome-wide expression analyses and retinal IHC, the ubiquitin-mediated proteolysis signal pathway may have participated in the induction retinal degeneration.
Chen Xie, Hong Zhu, Shuyang Chen, Yingying Wen, Le Jin, Liyue Zhang, Jianping Tong, Ye Shen
1772 related Products with: Chronic retinal injury induced by white LED light with different correlated color temperatures as determined by microarray analyses of genome-wide expression patterns in mice.96 assays96 samples1roll100 assays 100ul1 kit
#32738635 2020/07/29 To Up
Omeprazole, an inhibitor of proton pump, suppresses De novo lipogenesis in gastric epithelial cells.De novo lipogenesis (DNL) has been reported to involve in a serial types of disease. A standard triple therapy, including a PPI, omeprazole, and antibiotics (clarithromycin and amoxicillin), is widely used as the first-line regimen for helicobacter pylori (H. pylori)-infectious treatment. The objective of this study is to explore the function of a standard triple therapy on DNL.
Peiyu Chen, Linkai Li, Hongli Wang, Junhong Zhao, Yang Cheng, Jing Xie, Meiwan Cao, Ling Huang, Fangying Yang, Huan Chen, Jiayu Chen, Mingmin Su, Yuxin Xu, Fengfeng Zheng, Lanlan Geng, Wanfu Xu, Sitang Gong
1528 related Products with: Omeprazole, an inhibitor of proton pump, suppresses De novo lipogenesis in gastric epithelial cells.2 ml100 ul100 ul100ug Lyophilized20 3 mg100ug Lyophilized100ug Lyophilized5mg25 100ul
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