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#32473595   2020/05/30 To Up

Lowered endogenous mu-opioid receptor availability in subclinical depression and anxiety.

Major depressive disorder is associated with lowered mood, anxiety, anhedonia, sleep problems, and cognitive impairments. Many of these functions are regulated by μ-opioid receptor (MOR) system. Preclinical, in vivo, and post-mortem studies have however yielded inconclusive results regarding the role of the MOR in depression and anxiety. Moreover, it is not known whether alterations in MOR are already present in subclinical depression and anxiety. In a large-scale retrospective cross-sectional study we pooled data from 135 (113 males and 22 females) healthy subjects whose brain's MOR availability was measured with positron emission tomography (PET) using an agonist radioligand [C]carfentanil that has high affinity for MORs. Depressive and anxious symptomology was addressed with BDI-II and STAI-X questionnaires, respectively. Both anxiety and depression scores in the subclinical range were negatively associated with MOR availability in cortical and subcortical areas, notably in amygdala, hippocampus, ventral striatum, and orbitofrontal and cingulate cortices. We conclude that dysregulated MOR availability is involved in altered mood and pathophysiology of depression and anxiety disorders.
Lauri Nummenmaa, Tomi Karjalainen, Janne Isojärvi, Tatu Kantonen, Jouni Tuisku, Valtteri Kaasinen, Juho Joutsa, Pirjo Nuutila, Kari Kalliokoski, Jussi Hirvonen, Jarmo Hietala, Juha Rinne

1596 related Products with: Lowered endogenous mu-opioid receptor availability in subclinical depression and anxiety.

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#32473570   2020/05/27 To Up

Recombinant human ulinastatin improves immune dysfunction of dendritic cells in septic mice by inhibiting endoplasmic reticulum stress-related apoptosis.

Urinary trypsin inhibitor (UTI), also known as ulinastatin, has been reported to protect multiple organs against inflammation- and/or injury-induced dysfunction. In the present study, we aimed to investigate the immunomodulation effects of a recombinant human ulinastatin (urinary trypsin inhibitor, UTI) (rhUTI) on splenic dendritic cells (DCs) in cecal ligation and puncture (CLP)-induced septic mice. CLP mice were treated with rhUTI intramuscularly at 0, 12, and 24 h after procedure. Splenic CD11c DCs were isolated and accessed with flow cytometry for apoptotic or phenotypic analysis. Protein markers and cytokines were determined with Western blotting or ELISA. Treatment with rhUTI could markedly upregulate levels of costimulatory molecules (CD80, CD86) and MHC-II on surface of the splenic DC in CLP mice. The apoptotic rate of splenic DCs was decreased in CLP mice after rhUTI treatment. The survival rate of septic mice was increased after treatment with rhUTI. In addition, protein level of markers in endoplasmic reticulum stress (ERS)-related apoptotic pathways (including GRP78, caspase-12, and CHOP) were obviously down-regulated in the rhUTI-treated group when compared with the CLP group. These results indicate that rhUTI protects CLP-induced sepsis in mice by improving immune response of splenic DCs and inhibiting the excessive ERS-mediated apoptosis.
An-Long Qi, Yao Wu, Ning Dong, Yan-Fen Chai, Xiao-Mei Zhu, Yong-Ming Yao

1062 related Products with: Recombinant human ulinastatin improves immune dysfunction of dendritic cells in septic mice by inhibiting endoplasmic reticulum stress-related apoptosis.

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#32473569   2020/05/27 To Up

Detection of ctDNA with Personalized Molecular Barcode NGS and Its Clinical Significance in Patients with Early Breast Cancer.

We attempted to detect circulating tumor DNA (ctDNA), taking advantage of molecular barcode next-generation sequencing (MB-NGS), which can be more easily customized to detect a variety of mutations with a high sensitivity than PCR-based methods. Sequencing with a gene panel consisting of the 13 most frequently mutated genes in breast tumors from stage I or II patients revealed 95 somatic mutations in the 12 genes in 62% (62/100) of tumors. Then, plasma DNA from each patient (n = 62) before surgery was analyzed via MB-NGS customized to each somatic mutation, resulting in the detection of ctDNA in 16.1% (10/62) of patients. ctDNA was significantly associated with biologically aggressive phenotypes, including large tumor size (P = .004), positive lymph node (P = .009), high histological grade (P < .001), negative ER (P = .018), negative PR (P = .017), and positive HER2 (P = .046). Furthermore, distant disease-free survival was significantly worse in patients with ctDNA (n = 10) than those without ctDNA (n = 52) (P < .001). Our results demonstrate that MB-NGS personalized to each mutation can detect ctDNA with a high sensitivity in early breast cancer patients at diagnosis, and it seems to have a potential to serve as a clinically useful tumor marker for predicting their prognosis.
Tetsuhiro Yoshinami, Naofumi Kagara, Daisuke Motooka, Shota Nakamura, Tomohiro Miyake, Tomonori Tanei, Yasuto Naoi, Masafumi Shimoda, Kenzo Shimazu, Seung Jin Kim, Shinzaburo Noguchi

2984 related Products with: Detection of ctDNA with Personalized Molecular Barcode NGS and Its Clinical Significance in Patients with Early Breast Cancer.



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#32473567   2020/05/19 To Up

Illustrating the patient journey through the care continuum: Leveraging structured primary care electronic medical record (EMR) data in Ontario, Canada using chronic obstructive pulmonary disease as a case study.

Although barriers exist to secondary use of primary care electronic medical record (EMR) data, the Alliance for Healthier Communities (the Alliance) in Ontario, Canada has successfully created one of the largest structured primary care EMR datasets in Canada. In 2018, the Alliance and the Canadian Institute for Health Information (CIHI), an organization that provides comparable and actionable data to accelerate improvements in health across Canada, entered into a partnership to share EMR data. In this paper, we describe (i) the processes that enabled the collection of structured EMR data by the Alliance; (ii) how CIHI connected with the Alliance to share data and assess its quality; and, (iii) demonstrate the value of linking structured EMR data to administrative acute care data in illustrating the patient journey through the care continuum, using COPD as a case study.
Jennifer Rayner, Tanya Khan, Carmen Chan, Chen Wu

2565 related Products with: Illustrating the patient journey through the care continuum: Leveraging structured primary care electronic medical record (EMR) data in Ontario, Canada using chronic obstructive pulmonary disease as a case study.



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#32473560   2020/05/12 To Up

Two cadmium(II) coordination polymers as multi-functional luminescent sensors for the detection of Cr(VI) anions, dichloronitroaniline pesticide, and nitrofuran antibiotic in aqueous media.

Two ternary cadmium(II) coordination polymers, with the formulas being {[Cd(tptc)(bpz)(HO)]·0.5HO} (CP 1), and [Cd(tptc)(bpy)] (CP 2), were designed through mixed ligands strategy. Benefiting from the excellent chemical stability and luminescent property, two Cd(II) CPs possessing efficient multi-functional fluorescent responses toward Cr(VI) anions, 2,6-dichloro-4-nitroaniline pesticide, and nitrofuran antibiotic in aqueous media with high sensitivity, selectivity, and excellent recyclable behaviors with the detection limits (LODs) are 235 ppb for CrO anion, 343 ppb for CrO anion, 112 ppb for DCN pesticide, 62 ppb for NFT antibiotic for CP 1, and 173 ppb for CrO anion, 270 ppb for CrO anion, 638 ppb for DCN pesticide, 184 ppb for NFT antibiotic for CP 2, respectively. Besides, the mechanisms of luminescence quenching were revealed from the viewpoint of internal filter effect (IFE) and photoinduced electron transfer (PET).
Liming Fan, Feng Wang, Dongsheng Zhao, Xinhu Sun, Hongtai Chen, Huaiwei Wang, Xiutang Zhang

2752 related Products with: Two cadmium(II) coordination polymers as multi-functional luminescent sensors for the detection of Cr(VI) anions, dichloronitroaniline pesticide, and nitrofuran antibiotic in aqueous media.

100Tests250tests100 assays400 assays50 ml1 kit(96 Wells)100 μg400Tests

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#32473507   2020/05/16 To Up

Bamboo-biochar and hydrothermally treated-coal mediated geochemical speciation, transformation and uptake of Cd, Cr, and Pb in a polymetal(iod)s-contaminated mine soil.

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Mehr Ahmed Mujtaba Munir, Guijian Liu, Balal Yousaf, Muhammad Ubaid Ali, Ayesha Imtiyaz Cheema, Muhammad Saqib Rashid, Abdul Rehman

2129 related Products with: Bamboo-biochar and hydrothermally treated-coal mediated geochemical speciation, transformation and uptake of Cd, Cr, and Pb in a polymetal(iod)s-contaminated mine soil.

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#32473484   2020/05/20 To Up

A comparison of steroid and lipid binding cytochrome P450s from Mycobacterium marinum and Mycobacterium tuberculosis.

The steroid lipid binding cytochrome P450 (CYP) enzymes of Mycobacterium tuberculosis are essential for organism survival through metabolism of cholesterol and its derivatives. The counterparts to these enzymes from Mycobacterium marinum were studied to determine the degree of functional conservation between them. Spectroscopic analyses of substrate and inhibitor binding for the four M. marinum enzymes CYP125A6, CYP125A7, CYP142A3 and CYP124A1 were performed and compared to the equivalent enzymes of M. tuberculosis. The sequence of CYP125A7 from M. marinum was more similar to CYP125A1 from M. tuberculosis than CYP125A6 but both showed differences in the resting heme spin state and in the binding modes and affinities of certain azole inhibitors. CYP125A7 did not show a significant Type II inhibitor-like shift with any of the azoles tested. CYP142A3 bound a similar range of steroids and inhibitors to CYP142A1. However, there were some differences in the extent of the Type I shifts to the high-spin form with steroids and a higher affinity for the azole inhibitors compared to CYP142A1. The two CYP124 enzymes had similar substrate binding properties. M. marinum CYP124 was characterised by X-ray crystallography and displayed strong conservation of active site residues, except near the region where the carboxylate terminus of the phytanic acid substrate would be bound. As these enzymes in M. tuberculosis have been identified as candidates for inhibition the data here demonstrates that alternative strategies for inhibitor design may be required to target CYP family members from distinct pathogenic Mycobacterium species or other bacteria.
Stella A Child, Amna Ghith, John B Bruning, Stephen G Bell

1113 related Products with: A comparison of steroid and lipid binding cytochrome P450s from Mycobacterium marinum and Mycobacterium tuberculosis.

0.025 mg0.025 mg1000 TESTS/0.65ml100ug Lyophilized25 mg1 mL96T50 mg10 mg100ug Lyophilized1000 tests1000

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#32473445   2020/05/23 To Up

Biomedical article authorship: A proposal to enhance the meaningful assignment of authorship.

The authorship of articles in biomedical journals has proliferated despite efforts of publishers and editors to require justification of authorship. A proposal is made, herein, to resolve that matter by applying the "one paper: one citation" concept, so as to adhere to the thermodynamic principle of conservation of mass. This proposal provides (i) a means to allow authors to agree on their relative contribution, (ii) an incentive to assign only significant contributors to authorship, and (iii) the appropriate fractional contribution of each author when there are multiple authors. As a result, the sum of citations for any one paper shall be one paper. I believe this proposal the only method suggested, thus far, to make authorship of a biomedical paper authentic.
Marshall A Lichtman

1354 related Products with: Biomedical article authorship: A proposal to enhance the meaningful assignment of authorship.

1 mg1 mL 25 G100 g1 module1 kit(96 Wells)2.5 g

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#32473437   2020/05/11 To Up

Microbial sulfate reduction facilitates seasonal variation of arsenic concentration in groundwater of Jianghan Plain, Central China.

Bacterial sulfate reduction (BSR) plays a vital but complex role in regulating groundwater arsenic concentration. A quarterly hydro-biogeochemical investigation was conducted to clarify how BSR participated in arsenic dynamics in the geogenic As-contaminated alluvial aquifers of the Jianghan Plain, central Yangtze River Basin. Anthropogenic input of sulfate was identified in the transitional season with higher Cl concentrations and Cl/Br molar ratios compared to the monsoon season. Seasonal increase of S(-II) and Fe(II) concentrations in monsoon season suggests the co-occurrence of iron and sulfate reduction. Quantitative analysis of dsrB gene abundance revealed the corresponding variations between dsrB gene abundance (up to 1.2 × 10 copies L) and Fe(II) in groundwater. High-throughput sequencing of the dsrB gene identified a considerable proportion of sequences in the sulfate-reducing bacterial community was affiliated with Desulfobulbus (22.7 ± 20.8%) and Desulfocapsa (11.5 ± 11.9%). Moreover, the relative abundance of Desulfocapsa increased with the Fe(II) in the groundwater (R = 0.78, P < 0.01). These results suggest that microbially-mediated sulfate reduction facilitated the abiotic reduction of As-bearing Fe-oxides in the monsoon season after anthropogenic input of sulfate in the transitional season under oscillating redox conditions in the groundwater systems. The present research provides new insights into the critical role of BSR in the seasonal redox cycling of iron and variation of As in the aquifer systems, which are not only applicable in the central Yangtze River basin, but also to other similar As-rich alluvial aquifers worldwide.
Tianliang Zheng, Yamin Deng, Yanxin Wang, Hongchen Jiang, Xianjun Xie, Yiqun Gan

2170 related Products with: Microbial sulfate reduction facilitates seasonal variation of arsenic concentration in groundwater of Jianghan Plain, Central China.

100 UG1 mg 5 G10 mg10mg100.00 ug100 mg2.5 mg500

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