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Search results for: Human Epidermal Growth Factor (EGF) ELISA Kit

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#32524496   2020/06/10 To Up

Urinary biomarkers of inflammation and tissue remodeling may predict bladder dysfunction in patients with benign prostatic hyperplasia.

To evaluate the expression of urinary biomarkers of inflammation and tissue remodeling in patients with BPH undergoing surgery and evaluate the association of biomarkers with postoperative urodynamic outcomes MATERIALS AND METHODS: We analyzed urine samples from 71 patients treated with TURP from 2011 to 2017. Urinary levels of epidermal growth factor (EGF), matrix-metalloproteinase-1 (MMP-1), interleukin-6 (IL-6), nerve growth factor (NGF) and monocyte-chemoattractant protein-1 (MCP-1) (by commercial ELISA kit) were measured, adjusted by urinary creatinine (Cr) and analyzed according to patients clinical and urodynamic characteristics (baseline and 12-month postoperative urodynamic) RESULTS: MMP-1/Cr levels were significantly higher among subjects with higher detrusor pressure on preoprative urodynamic. MCP-1/Cr levels were significantly higher amongs subjects with preoperative DO. Preoperative levels of NGF/Cr (0.13 vs 0.08, p = 0.005) and MMP-1/Cr (0.11 vs 0.04, p = 0.021) were predictors of persistent DO 12 months after surgery. The following factors were shown to be useful for predicting the persistence of DO in the postoperative period: NGF/Cr, with an AUC of 0.77 (95% CI 0.62-0.92) (p = 0.006), and MMP-1/Cr, with an AUC of 0.72 (95% CI 0.56-0.88) (p = 0.022).
Paulo Sajovic de Conti, João Arthur Brunhara Alves Barbosa, Sabrina Thalita Reis, Nayara I Viana, Cristiano Mendes Gomes, Leonardo Borges, Marco Nunes, William C Nahas, Miguel Srougi, Alberto Azoubel Antunes

1265 related Products with: Urinary biomarkers of inflammation and tissue remodeling may predict bladder dysfunction in patients with benign prostatic hyperplasia.

100 μg

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#32051689   2020/01/21 To Up

Proximate Analysis and Safety Profile of Farmed Edible Bird's Nest in Malaysia and Its Effect on Cancer Cells.

Edible bird's nest (EBN) which is solidified swiftlet's saliva contains high nutritional value. It is widely consumed in countries like Malaysia, Indonesia, and Thailand. However, previous proximate analysis of Malaysia EBN was not representative of all the regions in Malaysia. In recent years, safety issues such as high nitrate and nitrite contents, presence of heavy metal, adulteration, fungal infection, and cancer cell stimulation were associated with EBN. Hence, this study aimed to determine the proximate analysis, safety profile during normal weather and hazy periods, and its effect on cancer cells stimulation in Malaysia-farmed EBN. Seven raw cleaned EBN samples were sourced from 6 different regions in Malaysia. Proximate analysis and safety profile were performed using official AOCA methods and Malaysian Standard. High protein (53.03-56.37%) and carbohydrate content (27.97-31.68%) with an acceptable level of moisture (10.8-14.04%) and ash (2.22-3.38%) were reported. A good safety profile was obtained with low nitrite and nitrate levels, with undetectable heavy metals and no significant growth of pathogenic microorganism except mould. Epidermal growth factor was detected but below the quantification level with the chicken EGF ELISA kit. The microculture tetrazolium (MTT) assay was performed for growth stimulation assessment comparing human EGF and EBN. There was no significant cell growth observed in cancer cells after EBN treatment. In conclusion, EBN Malaysia has a good nutritional profile, free of heavy metals, and an acceptable level of nitrate, nitrite, and microorganism profile except for mould contents. Furthermore, the study indicated that EBN was not associated with cancer cell growth.
Sin Nee Tan, Dahiru Sani, Chee Woei Lim, Aini Ideris, Johnson Stanslas, Christopher Thiam Seong Lim

2630 related Products with: Proximate Analysis and Safety Profile of Farmed Edible Bird's Nest in Malaysia and Its Effect on Cancer Cells.



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#31800535   // To Up

Regulatory cytokines prescribe the outcome of the inflammation in the process of pseudoexfoliation production.

The purpose of this study is to reveal the participation of different regulatory cytokines within the process of pseudoexfoliation (PEX).
Tatjana SarenacVulovic, Sladjana Pavlovic, Mitar Lutovac, Vladimir Zdravkovic, Suncica Sreckovic, Nemanja Zdravkovic

1033 related Products with: Regulatory cytokines prescribe the outcome of the inflammation in the process of pseudoexfoliation production.

111mg

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#29896251   2018/05/14 To Up

Overexpression of indoleamine 2, 3-dioxygenase contributes to the repair of human airway epithelial cells inhibited by dexamethasone via affecting the MAPK/ERK signaling pathway.

Indoleamine 2, 3-dioxygenase (IDO) catalyzes the degradation of trytophan, which serves a key role in immune suppression via regulating the production of several metabolites. The present study aimed to explore the effects and mechanisms of IDO in the repair of human airway epithelium suppressed by dexamethasone (DEX). Cell viability, proliferation and migration were evaluated using a Cell Counting Kit-8 (CCK-8), 5(6)-carboxyfluorescein diacetate succinimidyl ester (CFSE) labeling, and wound-healing assay, respectively. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR), western blot analysis and ELISA were performed to assess the levels of IDO, the mitogen-activated protein kinase (MAPK)/extracellular regulated kinase (ERK) pathway-related factors and epidermal growth factor (EGF) expression, respectively. The results revealed that overexpression of IDO enhanced the cell viability, and promoted the proliferation and migration of 16HBE cells which repair was inhibited by DEX. Furthermore, it was indicated that overexpression of IDO affected the MAPK/ERK pathway. In conclusion, overexpression of IDO promoted the human airway epithelium repair inhibited by DEX through affecting MAPK/ERK pathway. The present study implied that IDO may be a potential genetic therapeutic agent and supported the utilization of IDO in asthma.
Shanshan Jia, Pin Guo, Xiangjin Ge, Huanhuan Wu, Junhua Lu, Xiaofang Fan

2245 related Products with: Overexpression of indoleamine 2, 3-dioxygenase contributes to the repair of human airway epithelial cells inhibited by dexamethasone via affecting the MAPK/ERK signaling pathway.

1.00 flask25 TESTSTwo 96-Well Microplate Ki25 0.1 ml1mg100 ug/vial1.5 x 10^6 cells10

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#27636538   2016/09/16 To Up

An enzyme immunoassay for determining epidermal growth factor (EGF) in human serum samples using an ultramicroanalytical system.

Human epidermal growth factor is a small peptide consisting of 53 amino acid residues, which stimulates cell proliferation and is associated with several human carcinomas. A simple sandwich-type ultramicroELISA assay (UMELISA), based on the advantages of high affinity reaction between streptavidin and biotin has been developed for the measurement of EGF in human serum samples. Strips coated with a high affinity monoclonal antibody directed against EGF are used as solid phase, to ensure the specificity of the assay. The EGF assay was completed in 18 hr, with a measuring range of 39-2500 pg/mL. The intra- and inter-assay coefficients of variation were 4.4-7.3% and 0-5.1%, respectively, depending on the EGF concentrations evaluated. Percentage recovery ranged from 96-104%. Regression analysis showed a good correlation with the commercially available Human EGF Immunoassay Quantikine ELISA kit (n = 130, r = 0.92, P < 0.01). The analytical performance characteristics of our UMELISA EGF endorse its use for the quantification of EGF in human serum samples.
Elisa María Castells Martínez, Ruben Del Valle, Ernesto Carlos González, Antonio Melchor, Pedro Lucio Pérez, Idania González, Adriana Carr, Kalet León

2648 related Products with: An enzyme immunoassay for determining epidermal growth factor (EGF) in human serum samples using an ultramicroanalytical system.

4 Membranes/Box100 μg16 Arrays/Slide1 kit0.1 mg4 Sample Kit16 Arrays/Slide16 Arrays/Slide100.00 ug0.1ml (1mg/ml)8 Sample Kit16 Arrays/Slide

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#27321492   // To Up

[Effects of culture supernatant of human amnion mesenchymal stem cells on biological characteristics of human fibroblasts].

To investigate the effects of culture supernatant of human amnion mesenchymal stem cells (hAMSCs-CS) on biological characteristics of human fibroblasts.
Qi'er Wu, Lu Lyu, Haiming Xin, Liang Luo, Yalin Tong, Yongliang Mo, Yigang Yue

2222 related Products with: [Effects of culture supernatant of human amnion mesenchymal stem cells on biological characteristics of human fibroblasts].

100 extractions1 mg25 TESTS10 ug25 15ml1.00 flask1mg1.00 flask1.00 flask200

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#27188490   // To Up

[Effects of transfection of human epidermal growth factor gene with adenovirus vector on biological characteristics of human epidermal cells].

To investigate the suitable transfection condition of human epidermal cells (hECs) with human epidermal growth factor (EGF) gene by adenovirus vector (Ad-hEGF) and its effects on the biological characteristics of hECs.
Kai Yin, Li Ma, Chuan'an Shen, Yuru Shang, Dawei Li, Longzhu Li, Dongxu Zhao, Wenfeng Cheng

1506 related Products with: [Effects of transfection of human epidermal growth factor gene with adenovirus vector on biological characteristics of human epidermal cells].

50 ug5 x 50 ug5 x 50 ug1 kit(96 Wells)50 ug100ug1010ug50 ug10ug0.1 mg20 ug

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#27040853   2016/02/27 To Up

ER2, a novel human anti-EGFR monoclonal antibody inhibit tumor activity in non-small cell lung cancer models.

The epidermal growth factor receptor (EGFR) abnormalities including amplification, mutation, and overexpression are frequent in non-small cell lung cancer (NSCLC). We investigated in vitro and in vivo antitumor activity of ER2, a novel human anti-EGFR monoclonal antibody, in NSCLC.
Han Na Kang, Se-Ho Kim, Mi Ran Yun, Hye Ryun Kim, Sun Min Lim, Min-Soo Kim, Kwang-Won Hong, Sung-Moo Kim, Hwan Kim, Kyoung-Ho Pyo, Hye Ji Park, Joo Yeun Han, Hyun A Youn, Ki-Hwan Chang, Byoung Chul Cho

2217 related Products with: ER2, a novel human anti-EGFR monoclonal antibody inhibit tumor activity in non-small cell lung cancer models.

100ug Lyophilized100μg100uL100 ul100 ul50 ul100ug Lyophilized1mg

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#25907508   2015/04/24 To Up

Modulation of Regorafenib effects on HCC cell lines by epidermal growth factor.

Blood platelet numbers are correlated to growth and aggressiveness of several tumor types, including hepatocellular carcinoma (HCC). We previously found that platelet lysates (hPLs) also stimulated growth and migration, and antagonized the growth-inhibitory and apoptotic effects of both Sorafenib and Regorafenib, two multikinase inhibitors, on three HCC cell lines. In this study, in vitro function of human epidermal growth factor (EGF) with and without Sorafenib or Regorafenib was investigated.
Rosalba D'Alessandro, Maria Grazia Refolo, Catia Lippolis, Nicola Carella, Caterina Messa, Aldo Cavallini, Brian Irving Carr

2197 related Products with: Modulation of Regorafenib effects on HCC cell lines by epidermal growth factor.

5 x 50 ug50 ug100ug1 kit(96 Wells)5 x 50 ug11 kit(96 Wells)100.00 ug100ug96T50 ug

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#25717241   // To Up

Clinical significance and usefulness of soluble heparin binding-epidermal growth factor in gastric cancer.

To evaluate the clinical usefulness of soluble heparin-binding epidermal growth factor (sHB-EGF) as a serum biomarker for gastric cancer (GC).
Hye Won Chung, Hoon Young Kong, Jong-Baeck Lim

2123 related Products with: Clinical significance and usefulness of soluble heparin binding-epidermal growth factor in gastric cancer.

100.00 ug100.00 ug100.00 ug100.00 ug100.00 ug10ug

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