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Search results for: Rat IgM ELISA Kit

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#36808512   // To Up

[Effect of moxibustion on immune function homeostasis in rats with diarrhea irritable bowel syndrome based on SCF/c-kit signaling pathway].

To observe the effects of moxibustion on the stem cell factor (SCF)/tyrosine kinase receptor (c-kit) signaling pathway and immune function in rats with diarrhea irritable bowel syndrome (IBS-D), and to explore the mechanism of moxibustion for IBS-D.
Kui-Wu Li, Hao-Ran Chu, Jing-Ru Ruan, Jiao-Jiao Wang, Jin-Yu Chen, Shan-Shan Zhu, Ling Zou, Lu-Min Liao, Ting-Ting Tong

2107 related Products with: [Effect of moxibustion on immune function homeostasis in rats with diarrhea irritable bowel syndrome based on SCF/c-kit signaling pathway].

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#33667254   2021/03/05 To Up

Effects of GABA/β-glucan supplements on melatonin and serotonin content extracted from natural resources.

This study aimed to monitor the secretion of serotonin and melatonin in the blood serum of rats in the presence of rice bran (RB), and Sarcodon aspratus (S) extracts for sleep promotion.
Sayan Deb Dutta, Dinesh K Patel, Keya Ganguly, Ki-Taek Lim

1033 related Products with: Effects of GABA/β-glucan supplements on melatonin and serotonin content extracted from natural resources.

10 mg0.2 mg0.1 mg100 mg100ug0.1 ml1200ul100ug1000 10 mg100 mg

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#33600009   2021/02/18 To Up

Anti-phosphatidylserine/prothrombin complex antibodies in patients with cutaneous vasculitis: Possible involvement in the pathogenesis.

We assessed the IgG and IgM prevalence of anti-phosphatidylserine/prothrombin complex (aPS/PT) antibodies (Abs) in patients with vasculitis using a novel commercial ELISA kit. To examine whether aPS/PT Abs were involved in the pathogenesis of cutaneous vasculitis, inbred wild-type rats were intravenously administered with a rat IgM class aPS/PT monoclonal Ab established previously or with rat immunoglobulins as controls. To express PS on the surface of vascular endothelium, these rats were given a subcutaneous injection of cell-free histones in advance. Serum IgM aPS/PT Ab levels were elevated in patients with systemic vasculitis with skin involvement and cutaneous arteritis compared to those in patients with systemic vasculitis without skin involvement and healthy controls. There was no significant difference in the serum levels of IgG aPS/PT Abs between the patients and healthy controls. Correspondingly, inbred wild-type rats intravenously administered with the aPS/PT monoclonal IgM Ab after appropriate priming-subcutaneous histone injection developed cutaneous vasculitis. Some rats given rat IgM instead of the aPS/PT monoclonal Ab also developed cutaneous vasculitis, whereas vasculitis did not occur in rats given IgG or only priming by histones. We suggested that IgM aPS/PT Abs could be involved in the pathogenesis of cutaneous vasculitis based on these findings.
Tamihiro Kawakami, Yuto Tamura, Yupeng Dong, Miku Yoshinari, Yuka Nishibata, Sakiko Masuda, Utano Tomaru, Akihiro Ishizu

2769 related Products with: Anti-phosphatidylserine/prothrombin complex antibodies in patients with cutaneous vasculitis: Possible involvement in the pathogenesis.

100 μg100 μg1 mg100 μg100 μg0.2 mg100 μg100 μg100 μg100 μg100100 μg

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#21341161   // To Up

[Tularemia seroprevalence in the risky population living in both rural and urban areas of Erzurum].

Tularemia which is a zoonotic infection, caused by Francisella tularensis, has become a re-emerging disease in Turkey. Infection is often transmitted to human by handling animal tissues and products, but it is also possible to acquire the disease from contaminated water or food. Recently several cases and epidemics of tularemia have been reported in the northwest areas of Turkey, particularly in Marmara and West Black Sea regions. Erzurum is a city in Eastern Anatolia Region, Turkey and animal husbandry is the main agricultural activity in that area. However, neither tularemia cases were reported from this province nor seroprevalence studies were performed. In this study we aimed to determine F.tularensis antibody seropositivity in the risky population living at both rural and urban area of Erzurum. Blood samples from 240 volunteer subjects (134 male with mean age: 36.2, age range: 17-75 years and 106 female with mean age: 39.1, age range: 16-77 years) whose occupations were farming and animal husbandry, were included in the study. Serum samples were screened for the presence of F.tularensis antibodies by slide agglutination method (BD, USA) and Serazym ELISA kit (anti-F.tularensis IgG/IgA/IgM, Seramun, Germany). The positive samples with those tests were also retested by microagglutination test (MAT) in National Tularemia Reference Laboratory of Refik Saydam Hygiene Center, using antigen prepared in the same laboratory from the local strain. The serum samples were also searched for the presence of Brucella and Salmonella antibodies in terms of cross-reactivity. Seropositivity was detected in 71 (29.6%) out of 240 subjects by slide agglutination test (SAT), whereas only 5 (2.1%) gave positive result for total antibody by ELISA. Twenty-five of the 71 SAT positive samples yielded F.tularensis antibodies by MAT, of which 21 were between 1/20-1/40 and four were between 1/80-1/160 titers. However, all of the MAT positive samples (n= 25) were found reactive in Brucella and/or Salmonella antibody tests. One of the four MAT positive samples with 1/40 titer and all of the four MAT positive samples with ≥ 1/80 titer yielded positive results in ELISA. Since MAT gave very high cross reactive results, the five subjects (2.1%) found positive with ELISA were evaluated as seropositive for tularemia. Of those subjects (four were female, one was male; age range: 27-38 years), four were the inhabitants of the same village, and one from another neighboring village. All of the seropositive subjects were dealing with raising livestock and two were also farming. No history of contact with rat and wild animals or tick bite were detected, however it was noted that non-chlorinated fountain water has been used in both of these villages. In conclusion, our data emphasized that, populations inhabiting especially in rural area and dealing with farming and stock raising in our region are at risk for tularemia.
Halil Yazgı, M Hamidullah Uyanık, Mustafa Ertek, Selçuk Kılıç, Ekrem Kireçci, Kemalettin Ozden, Ahmet Ayyıldız

2675 related Products with: [Tularemia seroprevalence in the risky population living in both rural and urban areas of Erzurum].

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#9608269   // To Up

[Monoclonal antibodies in human hepatitis A virus immunoenzyme diagnosis].

Antigen and antibody detection in EIA is a good tool in diagnosing HAV infections, especially in their differentiation from other hepatitides. Commercial kits containing polyclonal antibodies and murine MAbs to identify HAV are now available. Rat MAbs have not been assayed so far. Peroxidase-labelled rat MAbs and purified rat MAbs as antigen capture were used to modify commercial "VectoHep A-IgM" and "VectorHep A-Ag" kits. The results obtained with modified "VectoHep A-IgM" and "VectorHep A-Ag" kits with labelled rat MAbs suggest that labelled rat MAbs can increase the sensitivity and specificity of EIA. MAbs used as antigen capture and labelled antibodies permit at least an 8-fold increase in sensitivity as compared to polyclonal antibodies. The modified "VectorHep A-Ag" kit with labelled rat MAbs provided 100% sensitivity and specificity in EIA. The modified "VectorHep A-Ag" kit also allowed the authors to determine viral antigens in the cell lysate, homogenates of the infected monkey liver, stools from patients, and sewage water samples. The rat MAbs modified kits can be recommended for using in epidemiological and clinical studies of HAV infections.
I A Razumov, Iu V Tumanov, E I Kazachinskaia, G I Tiunnikov, V B Loktev

2175 related Products with: [Monoclonal antibodies in human hepatitis A virus immunoenzyme diagnosis].

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