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#34118734   2021/05/27 To Up

Epidemiological feature, viral shedding, and antibody seroconversion among asymptomatic SARS-CoV-2 carriers and symptomatic/presymptomatic COVID-19 patients.

Novel coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is pandemic. However, data concerning the epidemiological features, viral shedding, and antibody dynamics between asymptomatic SARS-CoV-2 carriers and COVID-19 patients remain controversial.
Yi Chen, Ping Li, Yibo Ding, Miao Liu, Leijie Liu, Bo Yi, Ting Wu, Hongjun Dong, Xuying Lao, Keqing Ding, Haibo Wang, Dongliang Zhang, Xiaojie Tan, Zhongfa Wang, Guozhang Xu, Guangwen Cao

2498 related Products with: Epidemiological feature, viral shedding, and antibody seroconversion among asymptomatic SARS-CoV-2 carriers and symptomatic/presymptomatic COVID-19 patients.

200ug200ul200ug1,000 tests100ul100ug200ul100ug1000 tests100ul10 mg100ug

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#34118539   2021/06/05 To Up

Generation of recombinant antibodies by mammalian expression system for detecting S-metolachlor in environmental waters.

Current immunoassays for herbicide detection are usually based on polyclonal or monoclonal antibodies (MAbs) raised in animals. The mammalian expression system allows the procurement of specific and highly sensitive antibodies, avoiding animal immunization. In this study, S-metolachlor-specific IgG vectors bearing either Thosea asigna virus 2A or internal ribosome entry site (S-T2A or S-IRES) and single-chain variable fragment (scFv) vectors were designed and expressed. The recombinant antibodies (RAbs) were characterized by indirect competitive enzyme-linked immunosorbent assays (icELISA). The results showed that full-length RAbs exhibited significantly better performance than scFv, and both bicistronic vectors expressed antibodies of correct size, while RAb S-T2A elicited a higher yield than RAb S-IRES. Further analyses showed that RAb S-T2A and RAb S-IRES exhibited comparable reactivities and specificities to the parental MAb, with IC values of 3.44, 3.89 and 3.37 ng/mL, respectively. Finally, MAb- and RAb-based icELISAs were established for the determination of S-metolachlor in environmental waters. The recoveries were in the range of 73.0-128.1%, and the coefficients of variation were mostly below 10%. This article describes the production of RAbs for S-metolachlor from mammalian cells for the first time and paves the way to develop RAb-based immunoassays for monitoring herbicide residues in the environment.
Xiaoting Yu, Xu Zhang, Jingjing Xu, Pengyan Guo, Xiangmei Li, Hong Wang, Zhenlin Xu, Hongtao Lei, Xing Shen

2382 related Products with: Generation of recombinant antibodies by mammalian expression system for detecting S-metolachlor in environmental waters.

2x 100ug100 μg100 μg0.5 mg100 μg100 μg10010 100 μg100 μg4 Arrays/Slide

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#34118474   2021/06/09 To Up

High-throughput rat immunoglobulin G N-glycosylation profiling revealed subclass-specific changes associated with chronic stress.

Immunoglobulin G (IgG) glycosylation corresponds well with immune system changes, so it can potentially be used as a biomarker for the consequences of chronic stress such as low-grade inflammation and enhanced immunosenescence in older animals. Here we present a high-throughput glycoproteomic workflow, including IgG enrichment, HILIC glycopeptide purification, and nano-LC-MS analysis of tryptic glycopeptides applied for the analysis of rat IgG. A cohort of 80 animals was exposed to seven stressors in a customized chronic stress protocol with blood and tissue sampling in three timepoints. Young female rats experienced an increase in agalactosylated glycoforms on IgG2a and IgG2c accompanied by a decrease in monogalactosylation. Among old females, increased galactosylation was observed in the IgG2b subclass, pointing to an anti-inflammatory activity of IgG. Additionally, IgG Fc N-glycosylation patterns in Sprague Dawley rats were analyzed, quantified, and reported for the first time. Our findings emphasize age-, sex- and subclass-dependent differences in IgG glycosylation related to chronic stress exposure, confirming the relevance of newly developed methods for further research in glycobiology of rodent immune response. SIGNIFICANCE: In this study, we showed that a high-throughput streamlined methodology based on protein L 96-well monolithic plates for efficient rat IgG immunoaffinity enrichment from blood plasma, paired with appropriate tryptic glycopeptide preparation, HILIC-SPE enrichment, and nano-LC-MS methods was suitable for quick processing of large sample sets. We report a subclass-specific profiling and changes in rat IgG Fc galactosylation and adrenal gland immunohistochemistry of male and female animals exposed to a customized chronic stress protocol.
Siniša Habazin, Dražen Mlinarević, Marta Balog, Ana Bardak, Robert Gaspar, Kálmán Ferenc Szűcs, Sandor G Vari, Frano Vučković, Gordan Lauc, Mislav Novokmet, Marija Heffer

1492 related Products with: High-throughput rat immunoglobulin G N-glycosylation profiling revealed subclass-specific changes associated with chronic stress.

10, 10ml whole blood 4 Arrays/Slide64 Arrays/Slide100ml

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#34118427   2021/06/09 To Up

SARS-CoV-2 infection in properly vaccinated healthcare workers.

BNT162b2 mRNA Covid-19 vaccine has shown an optimal protection for population. However the development of SARS-CoV-2 infection has been reported in persons, who have received one or both doses of vaccine. The Hospital Universitario de Canarias began to vaccinate to healthcare workers on Febraury, 2021. We have detected several asymptomatic cases of SARS-CoV2 infections in healthcare workers.
Miriam Hernández Porto, Beatriz Castro, Zaida Diaz, Yanet Pedroso, María José Ramos, María Lecuona

1301 related Products with: SARS-CoV-2 infection in properly vaccinated healthcare workers.

96 tests2000 Units100 μg20000 Units5mg250ul200ul20ug

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#34118207   2021/06/09 To Up

Association between ocular toxoplasmosis and APEX1 and MYD88 polymorphism.

Ocular toxoplasmosis (OT) is the most common form of posterior uveitis, and in some countries, it is the most frequent cause of visual impairment. Studies demonstrate that the polymorphism in genes involved with the immune response can be related both to the occurrence and to the recurrence of OT. Thus, the present study aimed to analyze the association between OT and the polymorphism of the APEX1 (rs1130409) and MyD88 (rs7744) genes. The studied sample consisted of 48 volunteers with OT and 96 asymptomatic volunteers, but positive for anti - T. gondii IgG (control group). Blood collection was performed for serological analysis (ELISA) and DNA extraction. Genotyping of the polymorphism was performed using real-time PCR. To analyze the association between gene polymorphism and OT, logistic regression was performed. The results showed no association between the MYD88 gene polymorphism and the development of OT. However, a significant association was found between OT and APEX1 gene polymorphism, indicating that individuals expressing polymorphic (GG) or heterozygous (GT) alleles are more likely to develop the disease (P-value = 0.02 and 0.03 respectively). These results suggest that APEX1 (rs1130409) polymorphism is a risk factor for the occurrence of ocular toxoplasmosis in the studied population.
Débora de Almeida Aloise, Wendel Coura-Vital, Mariângela Carneiro, Marlus Venâncio Rodrigues, Gislani Acásia da Silva Toscano, Ramiza Bernardino da Silva, Rita de Cássia Barreto Silva-Portela, Fabrícia Lima Fontes-Dantas, Lucymara Fassarella Agnez-Lima, Ricardo Wagner Almeida Vitor, Valter Ferreira de Andrade Neto

2839 related Products with: Association between ocular toxoplasmosis and APEX1 and MYD88 polymorphism.

25 mg0.1 mg10 mg500 mg 5 G 5 G100ul25 mg1 g96 wells (1 kit)100ug100ug

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#34117873   2021/06/12 To Up

COVID-19 mRNA vaccination generates greater IgG levels in women compared to men.

Is antibody response to COVID-19 mRNA vaccination similar in women and men?
Alexis R Demonbreun, Amelia Sancilio, Matt E Velez, Daniel T Ryan, Lorenzo Pesce, Rana Saber, Lauren A Vaught, Nina L Reiser, Ryan R Hsieh, Richard T D'Aquila, Brian Mustanski, Thomas W McDade, Elizabeth M McNally

2259 related Products with: COVID-19 mRNA vaccination generates greater IgG levels in women compared to men.

100 ul0.1ml (1mg/ml)100ug0.1ml0.1ml200 ug25 1 mg100 µg0.1ml100 ug

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#34117221   2021/06/11 To Up

Distinct clinical and immunological profiles of patients with evidence of SARS-CoV-2 infection in sub-Saharan Africa.

Although the COVID-19 pandemic has left no country untouched there has been limited research to understand clinical and immunological responses in African populations. Here we characterise patients hospitalised with suspected (PCR-negative/IgG-positive) or confirmed (PCR-positive) COVID-19, and healthy community controls (PCR-negative/IgG-negative). PCR-positive COVID-19 participants were more likely to receive dexamethasone and a beta-lactam antibiotic, and survive to hospital discharge than PCR-negative/IgG-positive and PCR-negative/IgG-negative participants. PCR-negative/IgG-positive participants exhibited a nasal and systemic cytokine signature analogous to PCR-positive COVID-19 participants, predominated by chemokines and neutrophils and distinct from PCR-negative/IgG-negative participants. PCR-negative/IgG-positive participants had increased propensity for Staphylococcus aureus and Streptococcus pneumoniae colonisation. PCR-negative/IgG-positive individuals with high COVID-19 clinical suspicion had inflammatory profiles analogous to PCR-confirmed disease and potentially represent a target population for COVID-19 treatment strategies.
Ben Morton, Kayla G Barnes, Catherine Anscombe, Khuzwayo Jere, Prisca Matambo, Jonathan Mandolo, Raphael Kamng'ona, Comfort Brown, James Nyirenda, Tamara Phiri, Ndaziona P Banda, Charlotte Van Der Veer, Kwazizira S Mndolo, Kelvin Mponda, Jamie Rylance, Chimota Phiri, Jane Mallewa, Mulinda Nyirenda, Grace Katha, Paul Kambiya, James Jafali, Henry C Mwandumba, Stephen B Gordon, , Jennifer Cornick, Kondwani C Jambo

1520 related Products with: Distinct clinical and immunological profiles of patients with evidence of SARS-CoV-2 infection in sub-Saharan Africa.

20mg25ml100ug Lyophilized20ug

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#34116877   2021/06/08 To Up

Chimeric enterovirus 71 virus-like particle displaying conserved coxsackievirus A16 epitopes elicits potent immune responses and protects mice against lethal EV71 and CA16 infection.

Hand-foot-and-mouth disease (HFMD) is an infectious disease of infants and young children frequently caused by the enterovirus A species, mainly enterovirus 71 (EV71) and coxsackievirus A16 (CA16). In this study, we prepared the EV71 virus-like particle (EV71-VLP) and its chimeras using recombinant baculovirus (Bac-P1-3CD) co-expressing EV71 P1 (under polyhedrin promoter) and 3CD (under CMV-IE promoter) proteins in Sf9 cells. EV71-VLP chimera ChiEV71(1E)-VLP or ChiEV71(4E)-VLP displayed single CA16 PEP71 epitope in VP1 or four conserved CA16 neutralizing epitopes (PEP71 in VP1, aa136-150 in VP2, aa176-190 in VP3 and aa48-62 in VP4) by substitution of the corresponding regions of EV71 structure proteins, respectively. In mice, EV71-VLP and its chimeras elicited similar EV71-specific IgG and neutralizing antibody (NAb) titers compared to inactivated EV71. Expectedly, vaccination of ChiEV71(1E)-VLP or ChiEV71(4E)-VLP resulted in significantly increased CA16-specific IgG and NAb production and improved cross-protection against CA16 infection compared to EV71-VLP. Interestingly, the VLPs induced potent cellular immune responses and significantly decreased Th2 type (IL-4 and IL-10) cytokines secretion in the splenocytes of immunized mice compared to inactivated EV71 or inactivated CA16. Neonatal mice born to dams immunized with the chimeric VLPs or neonatal mice passively transferred with sera of immunized mice were completely protected from lethal EV71 challenge and partially protected from lethal CA16 infection. Our study provides a novel bivalent or multivalent vaccine strategy to prevent EV71 and related-enterovirus infections.
Jin Luo, Chunling Huo, Huan Qin, Junhong Hu, Lei Lei, Zishu Pan

1491 related Products with: Chimeric enterovirus 71 virus-like particle displaying conserved coxsackievirus A16 epitopes elicits potent immune responses and protects mice against lethal EV71 and CA16 infection.

1 mg25 mg10 mg500 mg100ul50 ug 5mg100ul25 mg96T100ug25 mg

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#34116710   2021/06/11 To Up

Development of a rapid and sensitive immunochromatographic strip based on EuNPs-ES fluorescent probe for the detection of early Trichinella spiralis-specific IgG antibody in pigs.

Trichinellosis, which is caused by nematodes of the genus Trichinella, is one of the most important zoonotic parasite diseases in the world. A rapid and sensitive immunochromatographic strip (ICS) based on Eu (III) nanoparticles (EuNPs) was developed for the detection of Trichinella spiralis (T. spiralis) infection in pigs. T. spiralis muscle larvae excretory secretory or preadult worm excretory secretory (ML-ES or PAW-ES) antigens were conjugated with EuNPs probes to capture T. spiralis-specific antibodies in pig sera, after which the complex bound to mouse anti-pig IgG deposited on the test line (T-line), producing a fluorescent signal. In the pigs infected with 100, 1000 and 10 000 ML, seroconversion was first detectable for the EuNPs-ML-ES ICS at 30, 25 and 21 days post-infection (dpi) and for the EuNPs-PAW-ES ICS at 25, 21 and 17 dpi. These results show that EuNPs-PAW-ES ICS detects anti-Trichinella IgG in pigs 4-5 days earlier that test using ML-ES antigens. Our ICS have no cross reaction with other parasite infection sera. Furthermore, the detection process could be completed in 10 min. This study indicated that our ICS can be used for the detection of the circulating antibodies in early T. spiralis infection and provide a novel method for on-site detection of T. spiralis infection in pigs.
Xinyu Wang, Bin Tang, Ying Zhao, Jing Ding, Nan Wang, Yan Liu, Zijian Dong, Xiangdong Sun, Quangang Xu, Mingyuan Liu, Xiaolei Liu

1233 related Products with: Development of a rapid and sensitive immunochromatographic strip based on EuNPs-ES fluorescent probe for the detection of early Trichinella spiralis-specific IgG antibody in pigs.

0.2 mg0.25 mg100 TESTS1 ml4 Membranes/Box4 Membranes/Box4 Arrays/Slide4 Arrays/Slide4 Membranes/Box4 Membranes/Box

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