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Defining lncRNAs Correlated with CHO Cell Growth and IgG Productivity by RNA-Seq.

How the long non-coding RNA (lncRNA) genome in recombinant protein producing Chinese hamster ovary (CHO) cell lines relates to phenotype is not well described. We therefore defined the CHO cell lncRNA transcriptome from cells grown in controlled miniature bioreactors under fed-batch conditions using RNA-Seq to identify lncRNAs and how the expression of these changes throughout growth and between IgG producers. We identify lncRNAs including Adapt15, linked to ER stress, GAS5, linked to mTOR signaling/growth arrest, and PVT1, linked to Myc expression, which are differentially regulated during fed-batch culture and whose expression correlates to productivity and growth. Changes in (non)-coding RNA expression between the seed train and the equivalent day of fed-batch culture are also reported and compared with existing datasets. Collectively, we present a comprehensive lncRNA CHO cell profiling and identify targets for engineering growth and productivity characteristics of CHO cells.

2270 related Products with: Defining lncRNAs Correlated with CHO Cell Growth and IgG Productivity by RNA-Seq.

High density (208 core) t anti HSV (II) gB IgG1 (mo Epidermal Growth Factor ( Human Growth Hormone anti anti CD37 IgG2b (monoclon RABBIT ANTI HUMAN SDF-1 A Mouse AntiT cell receptor anti HCMV gB IgG1 (monocl Mouse Vascular Endothelia CELLKINES PLATELET DERIVE Mouse Anti DO11.10 T cell Human Growth Hormone anti

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Polyglycidol-Stabilized Nanoparticles as a Promising Alternative to Nanoparticle PEGylation: Polymer Synthesis and Protein Fouling Considerations.

We herein demonstrate the outstanding protein-repelling characteristic of star-like micelles and polymersomes manufactured from amphiphilic block copolymers made by poly(butylene oxide) (PBO) hydrophobic segments and polyglycidol (PGL) hydrophilic outer shells. Although positively charged proteins (herein modeled by lysozyme) may adsorb onto the surface of micelles and polymersomes where the assemblies are stabilized by short PGL chains (degree of polymerization smaller than 15), the protein adsorption vanishes when the degree of polymerization of the hydrophilic segment (PGL) is higher than ~ 20, regardless the morphology. This has been probed by using three different model proteins which are remarkably different concerning molecular weight, size and zeta potential (bovine serum albumin - BSA, lysozyme and immunoglobulin G - IgG). Indeed, the adsorption of the most abundant plasma protein (herein modeled as BSA) is circumvented even by using very short PGL shells due to the highly negative zeta potential of the produced assemblies which presumably promotes protein-nanoparticle electrostatic repulsion. The negative zeta potential, on the other hand, enables lysozyme adsorption and the phenomenon is governed by electrostatic forces as evidenced by isothermal titration calorimetry. Nevertheless, the protein coating can be circumvented by slightly increasing the degree of polymerization of the hydrophilic segment. Notably, the PGL length required to circumvent protein fouling is significantly smaller than the one required for PEO. This feature and the safety concerns regarding the synthetic procedures on the preparation of poly(ethylene oxide)-based amphiphilic copolymers might make polyglycidol a promising alternative towards the production of non-fouling spherical particles.

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MarkerGene™ Total Prote Total Rat tPA Antigen Ass Anti ASXL1(Putative Polyc Glutathione (GSH GSSG Tot Recombinant Human IFN-alp Rabbit Anti-Rat Androgen Toxoplasma gondii GRA8, r EpiQuik General Protein D Total Mouse PAI-1 Antigen Mouse Anti-Astrovirus Cap NWLSS™ TAC Peroxyl Assa BCA Protein Quantitation

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Non-anticoagulant heparin as a pre-exposure prophylaxis prevents Lyme disease infection.

Lyme disease (LD) is caused by the spirochete Borrelia burgdorferi sensu lato (Bbsl). After transmission to humans by ticks, Bbsl spreads to multiple organs, leading to arthritis, carditis, and neuroborreliosis. No effective prophylaxis against human LD prior to tick exposure is currently availa-ble. Thus, a pre-exposure prophylaxis (PrEP) against LD is needed. The establishment of LD bacteria at diverse sites is dictated partly by the binding of Bbsl to proteoglycans (PGs) and glycosaminoglycans (GAGs) in tissues. The drug heparin is structurally similar to these GAGs and inhibits Bbsl attach-ment to PGs, GAGs, cells, and tissues, suggesting its potential to prevent LD. However, the anticoagulant activity of heparin often results in hemorrhage, hampering the development of this compound as LD PrEP. We have previously synthesized a non-anticoagulant version of heparins (NACHs), which was verified for safety in mice and humans. Here, we showed that NACH blocks Bbsl attachment to PGs, GAGs, and mammalian cells. We also found that treating mice with NACH prior to the exposure of ticks carrying Bbsl followed by continuous admin-istration of this compound prevents tissue colonization by Bbsl. Furthermore, NACH-treated mice develop greater levels of IgG and IgM against Bbsl at early stages of infection, sug-gesting that the upregulation of antibody immune responses may be one of the mechanisms for NACH-mediated LD pre-vention. This is one of the first studies examining the ability of a heparin-based compound to prevent LD prior to tick ex-posure. The information presented might also be extended to prevent other infectious diseases agents.

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ENZYMATIC ASSAY KITS (CH Rabbit Anti-B. burgdorfer ENZYMATIC ASSAY KITS (CH Rabbit Anti-B. burgdorfer Mouse Anti-B. burgdorferi ENZYMATIC ASSAY KITS (CH Rabbit Anti-B. burgdorfer ENZYMATIC ASSAY KITS (CH Purified Rabbit Anti Huma Cell Meter™ Caspase 9 A Caspase-2 Substrate VDVAD HDAC Colorimetric Activit

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Hepatitis E Virus in Pigs from Slaughterhouses, United States, 2017-2019.

Hepatitis E virus (HEV) RNA was detected in 6.3% and HEV IgG in 40% of 5,033 serum samples from market-weight pigs at 25 slaughterhouses in 10 US states. The prevalent HEV genotype was zoonotic genotype 3, group 2. Blood of HEV-viremic pigs from slaughterhouses may contaminate pork supply chains.

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Human anti hepatitis A vi Human Epstein-Barr Virus Mouse Epstein-Barr Virus Rabbit Anti-Polyprotein(H Avian Influenza virus H5N Human Anti-E Antigen of H Avian Influenza virus H5N Human E Antigen of Hepati Human Anti-Core Antigen o Mouse AntiInfluenza B Nuc Goat Anti-Influenza A Vir Recombinant Dengue Virus

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Antibody targeted PET Imaging of 64Cu-DOTA-Anti-CEA PEGylated Lipid Nanodiscs in CEA positive tumors.

Lipid nanodiscs (LNDs), comprising a phospholipid bilayer encircled by two molecules of a recombinant membrane scaffold protein, can be targeted to tumors with covalently attached antibodies (Abs) or their fragments. Antibody attachment to click chemistry based PEGylated lipids on LNDs including DOTA allowed PET imaging with the positron emitter 64Cu. Carcinoembryonic antigen (CEA) positive tumors in CEA transgenic mice were chosen as a tumor target. Fab' fragments, that otherwise are rapidly cleared by the kidney due to their small size, were retained in circulation when conjugated to LNDs. Untargeted PET imaging of 64Cu-DOTA-LNDs revealed low tumor uptake (4-5 %ID/g) in the range expected for the enhanced permeability retention (EPR) effect with high liver uptake (17-21 %ID/g) indicating gut clearance. Fab'-targeted LNDs showed little improvement over untargeted LNDs, but intact IgG targeted LNDs gave high tumor uptake (40 %ID/g) with low liver (8 %ID/g), demonstrating that tumor targeting with antibody conjugated LNDs is feasible.

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Evaluation of serological diagnostic tests of human brucellosis for prevention and control in Mexico.

Brucellosis is a zoonosis mainly present in developing countries. The WHO reports 500,000 new cases every year. From 2012 to 2016, 13,677 cases were reported in Mexico, with 2.00 to 2.64 rate per 100,000 inhabitants. To analyze the diagnostic algorithm of brucellosis in Mexico, we compared the commercial laboratory tests ELISA, Brucellacapt®, and lateral flow test (LFT) in a study of 473 individuals from two endemic Mexican populations. All patients were treated in first-level medical units for presenting brucellosis compatible symptoms and without a history of the disease. Clinical-epidemiological information was gathered and initial serum samples were obtained to react with anti-Brucella antibodies; subsequent samples were collected at follow-up treatment visits. Using the Rose Bengal screening, we found 165 negative samples and 308 positive reactive samples, of which 222 cases were confirmed and 234 were positive on at least one marker (IgG or IgM) or LFT. When Brucellacapt® was used, similar results to those observed with the conventional algorithm were found as judged by the Cohen's kappa coefficient (κ) (0.813, 95% CI 0.7788-0.8472). Similar κ indices between conventional algorithm and ELISA pair were found, 0.7038 (95% CI 0.6555-0.7521), representing high similarity between both groups of diagnosis. We conclude that conventional serodiagnoses, Brucellacapt® and LFT, presented inconclusive results and poor correlation between them. By contrast, ELISA test pair (IgG + IgM) presented high correlation with the conventional algorithm and greater capacity for correct positive and negative classification.

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MOUSE ANTI HUMAN CD19 RPE Beta Amyloid (1 40) ELISA Beta Amyloid (40) ELISA K Beta Amyloid (42) ELISA K Leptin ELISA Kit, Rat Lep Beta Amyloid (1 40) ELISA Mouse Anti-Human Interleu Mouse Anti-Human CD14, AP Mouse Anti-Human CD95, RP Goat Anti-Human GCNT3 (aa Sterile filtered human se Mouse Anti-Human CD21

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Women who received varicella vaccine versus natural infection have different long-term T cell immunity but similar antibody levels.

Varicella-zoster virus (VZV) infection during pregnancy is associated with serious fetal anomalies. The live-attenuated VZV vaccine was approved in 1995, so many vaccinated women are now of childbearing age. The question of long-term immunity to varicella is critical because breakthrough chickenpox can occur after vaccination.

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Anti-BRF1(Butyrate respon BAFF (B cell activating f 1,1'-Dioctadecyl-3,3,3',3 Anti-Beclin 1 (N-terminal Anti-ADAMTS-2, C-Terminal Anti-ADAM-8, C-Terminal p Interleukin-34 IL34 (N-t ALDH1A1 (C Terminus) Anti Small cell lung carcinoma BAG5 (N Terminus) Antibod Rabbit Anti-PARP (N-Termi Rabbit Anti-Tenascin C (C

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Development and optimization of OspC chimeritope vaccinogens for Lyme disease.

Experimental Outer surface protein (Osp) C based subunit chimeritope vaccinogens for Lyme disease (LD) were assessed for immunogenicity, structure, ability to elicit antibody (Ab) responses to divergent OspC proteins, and bactericidal activity. Chimeritopes are chimeric epitope based proteins that consist of linear epitopes derived from multiple proteins or multiple variants of a protein. An inherent advantage to chimeritope vaccinogens is that they can be constructed to trigger broadly protective Ab responses. Three OspC chimeritope proteins were comparatively assessed: Chv1, Chv2 and Chv3. The Chv proteins possess the same set of 18 linear epitopes derived from 9 OspC type proteins but differ in the physical ordering of epitopes or by the presence or absence of linkers. All Chv proteins were immunogenic in mice and rats eliciting high titer Ab. Immunoblot and enzyme linked immunosorbent assays demonstrated that the Chv proteins elicit IgG that recognizes a diverse array of OspC type proteins. The panel included OspC proteins produced by N. American and European strains of the LD spirochetes. Rat anti-Chv antisera uniformly labeled intact, non-permeabilized Borreliella burgdorferi demonstrating that vaccinal Ab can bind to targets that are naturally presented on the spirochete cell surface. Vaccinal Ab also displayed potent complement dependent-Ab mediated killing activity. This study highlights the ability of OspC chimeritopes to serve as vaccinogens that trigger potentially broadly protective Ab responses. In addition to the current use of an OspC chimeritope in a canine LD vaccine, chimeritopes can serve as key components of human LD subunit vaccines.

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Rabbit Anti-B. burgdorfer Rabbit Anti-B. burgdorfer Mouse Anti-B. burgdorferi Rabbit Anti-B. burgdorfer 4 Androstene 3,17 dione C 2 Formyl imidazole (Imida Chicken craniofacial deve Lung disease tissue array Androgen Receptor (Phosph QuantiChrom™ Formaldehy Benzyl (2S,3S,5S)-2-Hexyl Mouse Anti-B. burgdorferi

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Molecular characterization and tissue localization of glutathione -transferase from adult .

Glutathione S-transferases (GSTs) are a detoxifying enzyme family that is essential for parasite blood-feeding and survival, and represent potential targets for hookworm vaccine development. Multiple GST-encoding complementary DNAs (cDNAs) have been cloned from Ancylostoma caninum and Necator americanus, but there are no reports about the cloning of this enzyme from Ancylostoma ceylanicum, the animal-derived zoonotic hookworm. To study the molecular nature and tissue localization of GST of A. ceylanicum (Ace-GST), we designed primers based on the GST gene sequence of A. ceylanicum in GenBank, amplified the Ace-GST cDNA by reverse transcription polymerase chain reaction, and analysed its homology and genetic evolution relationship. The amplified product was cloned into the pET-32a vector and transformed into Escherichia coli BL21 (DE3) for expression. To prepare anti-GST polyclonal antibodies, the recombinant protein was purified and used to immunize Kunming mice. The level of immunoglobulin G (IgG) antibody in the serum of immunized mice was detected by indirect enzyme-linked immunosorbent assay, and the Ace-GST localization in adult worm was determined using the immunofluorescence method. The results showed that the full-length cDNA encoding Ace-GST was 468 bp, which had the highest homology with Ac-GST-1 (60.1%) and clustered into one branch (v-class) with Ac-GST-1 and Na-GST-1 in a phylogenetic tree. Mice immunized with recombinant Ace-GST showed specific IgG antibody response. Immunolocalization revealed that natural Ace-GST is mainly located in the epidermis, muscle and intestine of the adult. These results may lay a foundation for further studies on the biological function of Ace-GST.

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Reduced progression of bone erosion in cytomegalovirus seropositive rheumatoid arthritis patients.

Human cytomegalovirus (HCMV) seropositivity has been associated with higher inflammation during rheumatoid arthritis (RA). However, no data are available on the impact of HCMV seropositivity on bone erosion progression during RA.

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Bone marrow tumor and nor Pancreatic disease spectr Small intestine disease ( Brain disease spectrum (b Bone cancer test tissue a Ovary disease spectrum (o Kidney disease spectrum ( Bone disease spectrum (bo Bone and cartilage diseas Bone marrow tumor and adj Human normal bone and ost Bone and cartilage cancer

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