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Sustainable, healthy cities: protocol of a mixed methods evaluation of a cluster randomized controlled trial for Aedes control in Brazil using a community mobilization approach.

Dengue is increasing in its global presence with an estimated 4 billion people at-risk of infection in at least 128 countries. Despite the promising results of EcoHealth and community mobilization approaches to Aedes reduction, more evidence of their efficacy on reducing dengue risk is needed. The principal research question is to determine if interventions based upon community mobilization reduce the risk of dengue virus infection among children 3 to 9 years old compared to usual dengue control practice in Fortaleza, Brazil.

1201 related Products with: Sustainable, healthy cities: protocol of a mixed methods evaluation of a cluster randomized controlled trial for Aedes control in Brazil using a community mobilization approach.

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Evaluation of the Immunoprotective Potential of Recombinant Paraflagellar Rod Proteins of in Mice.

Trypanosomosis, caused by , is an economically significant disease of livestock. Systematic antigenic variation by the parasite has undermined prospects for the development of a protective vaccine that targets the immunodominant surface antigens, encouraging exploration of alternatives. The paraflagellar rod (PFR), constituent proteins of the flagellum, are prominent non-variable vaccine candidates for owing to their strategic location. Two major PFR constituent proteins, PFR1 (1770bp) and PFR2 (1800bp), were expressed using . Swiss albino mice were immunized with the purified recombinant TePFR1 (89KDa) and TePFR2 (88KDa) proteins, as well as with the mix of the combined proteins at equimolar concentrations, and subsequently challenged with virulent . The PFR-specific humoral response was assessed by ELISA. Cytometric bead-based assay was used to measure the cytokine response and flow cytometry for quantification of the cytokines. The recombinant TePFR proteins induced specific humoral responses in mice, including IgG1 followed by IgG2a and IgG2b. A balanced cytokine response induced by rTePFR 1 and 2 protein vaccination associated with extended survival and improved control of parasitemia following lethal challenge. The observation confirms the immunoprophylactic potential of the covert antigens of .

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Biological risk assessment of miltefosine in concomitant infection with opportunistic toxoplasmosis.

Although miltefosine is the first line for treatment of leishmaniasis, it could have multiple un-recognized effects if any infection accidentally takes place during therapy. The aim is to precisely evaluate the molecular and biochemical remarks of miltefosine on Toxoplasma gondii accidental infection during miltefosine therapeutic course.

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The reduction in the immunoglobulin G and immunoglobulin E binding capacity of β-lactoglobulin via spray-drying technology.

Bovine β-lactoglobulin (β-LG) is the major allergen in milk powder. The IgG/IgE binding capacity and structural characteristics of β-LG after spray drying in the presence or absence of α-lactose at 120 and 180°C were investigated by ELISA and mass spectrometry. At a drying temperature of 120°C, no change was found in the IgG/IgE binding capacity of β-LG and no change was observed in free amino group content, fluorescence intensity, or detectable glycation. At a drying temperature of 180°C, aggregation of β-LG occurred, leading to a decrease in the IgG/IgE binding capacity. When α-lactose was also present, 7 lysine side-chains in β-LG were modified by glycation and the IgG/IgE binding capacity was further decreased. Therefore, the glycation and structural changes in β-LG were responsible for the reduction in the IgG/IgE binding capacity during high temperature (180°C) spray drying.

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Measles antibody levels among vaccinated and unvaccinated children 6-59 months of age in the Democratic Republic of the Congo, 2013-2014.

Measles is endemic in the Democratic Republic of the Congo (DRC), and 89-94% herd immunity is required to halt its transmission. Much of the World Health Organization African Region, including the DRC, has vaccination coverage below the 95% level required to eliminate measles, heightening concern of inadequate measles immunity.

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Protective immunity elicited by the nematode-conserved As37 recombinant protein against Ascaris suum infection.

Ascaris lumbricoides is one of the three major soil-transmitted gastrointestinal helminths (STHs) that infect more than 440 million people in the world, ranking this neglected tropical disease among the most common afflictions of people living in poverty. Children infected with this roundworm suffer from malnutrition, growth stunting as well as cognitive and intellectual deficits. An effective vaccine is urgently needed to complement anthelmintic deworming as a better approach to control helminth infections. As37 is an immunodominant antigen of Ascaris suum, a pig roundworm closely related to the human A. lumbricoides parasite, recognized by protective immune sera from A. suum infected mice. In this study, the immunogenicity and vaccine efficacy of recombinant As37 were evaluated in a mouse model.

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Affinity of Streptococcal G Protein to Forest Musk Deer () Serum Immunoglobulin G.

Forest musk deer (FMD; ) immunoglobulin G efficiently bound to streptococcal G protein (SPG) and weakly bound to staphylococcal A protein. The results suggested that horseradish peroxidase-conjugated SPG could be chosen as an enzyme-labeled antibody substitute and laid a foundation for immunologic research in FMD disease.

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Interference of M-protein on Thrombin Time Test: A Case Report.

A case of interference of monoclonal protein (M-protein) on thrombin time (TT) test in a 39-year-old Caucasian male patient is presented.

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