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Search results for: IgG2a

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#33072807   2020/08/22 To Up

Child HIV Exposure and CMV Seroprevalence in Botswana: No Associations With 24-Month Growth and Neurodevelopment.

We sought to identify predictors of child cytomegalovirus (CMV) infection overall and by maternal HIV status and to assess associations of child CMV status with growth and neurodevelopmental outcomes at 24 months of age in Botswana.
Natasha O Moraka, Sikhulile Moyo, Christiana Smith, Maryanne Ibrahim, Gloria Mayondi, Jean Leidner, Kathleen M Powis, Adam R Cassidy, Betsy Kammerer, Gbolahan Ajibola, Paige L Williams, Adriana Weinberg, Rosemary Musonda, Roger Shapiro, Simani Gaseitsiwe, Shahin Lockman

1959 related Products with: Child HIV Exposure and CMV Seroprevalence in Botswana: No Associations With 24-Month Growth and Neurodevelopment.

10 mg

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#33072639   2020/07/27 To Up

Prevalence of Infection among Type 2 Diabetes Mellitus.

Several conducted studies have reported a higher and more frequent infection rate in type 2 diabetes mellitus (T2DM). The aim of this study was to investigate the prevalence and its association between infection and T2DM.
Abdulbari Bener, Ahmet Faruk Ağan, Abdulla O A A Al-Hamaq, Cem Cahit Barisik, Mustafa Öztürk, Abdulkadir Ömer

1294 related Products with: Prevalence of Infection among Type 2 Diabetes Mellitus.

200ul200ul5/100 Packing /sleeve/bo200ul200ul200ul1200ul200ul1

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#33072516   2020/10/03 To Up

Anti-drug antibody formation in Japanese Fabry patients following enzyme replacement therapy.

Enzyme replacement therapy (ERT) for Fabry disease (deficiency of α-galactosidase A, α-Gal) with recombinant α-Gals (agalsidase alfa and agalsidase beta) is widely available and improves some of the clinical manifestations and biochemical findings. However, recent reports suggest that recurrent administration of recombinant enzymes often induces the formation of anti-drug antibodies, which may have a negative impact on the outcome of the therapy. We examined the formation of anti-drug antibodies using blood samples from 97 Japanese Fabry patients following ERT and tried to characterize them by means of enzyme-linked immunosorbent assay (ELISA), serum-mediated α-Gal inhibition, and immunochromatographic (IC) assay, followed by gene analysis and measurement of plasma globotriaosylsphingosine (lyso-Gb3). ELISA revealed that 20/35 (57%) classic Fabry males were antibody (Immunoglobulin G, IgG) -positive (Ab+) at 6 months after the initiation of ERT, although only two of the seventeen (12%) later-onset Fabry males and none of the 45 Fabry females were. The Ab+ state was maintained at least until 24 months after the initiation of ERT in most of the cases, the exceptions being two patients who acquired immune tolerance during ERT. As many Ab+ patients have nonsense mutations, attention should be paid to the formation of anti-drug antibodies in Fabry patients harboring such gene mutations, who hardly produce α-Gal protein. Serum-mediated α-Gal inhibition was seen in most of the Ab+ patients and the antibodies affected the reduction of the plasma lyso-Gb3 level following ERT, suggesting that the antibodies inhibit the enzyme activity. There was a correlation between the results of the IC test and those of the ELISA. As the former is easy and rapid, it should be useful as a bed-side test.
Takahiro Tsukimura, Yuya Tayama, Tomoko Shiga, Kanako Hirai, Tadayasu Togawa, Hitoshi Sakuraba

2319 related Products with: Anti-drug antibody formation in Japanese Fabry patients following enzyme replacement therapy.

100μg100ug100ug Lyophilized100ug100ug100ug Lyophilized100ug100ug100ug Lyophilized100ug Lyophilized100ug100ug Lyophilized

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#33072110   2020/09/23 To Up

Investigating Immunization With Nucleotide Enzymes of : Nucleoside Diphosphate Kinase and Adenylosuccinate Lyase as New Antigenic Targets Against Schistosomiasis.

Schistosomiasis, caused by trematode worm, affects more than 1.5 million people in Brazil. The current treatment consists in the administration of Praziquantel, the only medicine used for treatment for more than 40 years. Some of the limitations of this drug consist in its inactivity against schistosomula and parasite eggs, the appearance of resistant strains and non-prevention against reinfection. Thus, the objective of this study was to evaluate the effect of immunization with recombinant functional enzymes of the purine salvage pathway of , Nucleoside Diphosphate Kinase (NDPK) and Adenylosuccinate Lyase (ADSL), to evaluate the host immune response, as well as the parasite load after vaccination. For this, Balb/c mice were divided into 5 groups: control (uninfected and untreated), non-immunized/infected, NDPK infected, ADSL infected, and NDPK + ADSL infected. Immunized groups received three enzyme dosages, with a 15-day interval between each dose, and after 15 days of the last application the animals were infected with 80 cercariae of . On the 47th day after the infection, fecal eggs were counted and, on the 48th day after the infection, the evaluation of leukocyte response, parasite load, antibody production, cytokines quantification, and histopathological analysis were performed. The results showed that immunizations with NDPK, ADSL or NDPK + ADSL promoted a discreet reduction in eosinophil counts in lavage of peritoneal cavity. All immunized animals showed increased production and secretion of IgG1, IgG2a, and IgE antibodies. Increased production of IL-4 was observed in the group immunized with the combination of both enzymes (NDPK + ADSL). In addition, in all immunized groups there were reductions in egg counts in the liver and intestine, such as reductions in liver granulomas. Thus, we suggest that immunizations with these enzymes could contribute to the reduction of schistosomiasis transmission, besides being important in immunopathogenesis control of the disease.
Túlio di Orlando Cagnazzo, Camila Tita Nogueira, Cynthia Aparecida de Castro, Débora Meira Neris, Ana Carolina Maragno Fattori, Ricardo de Oliveira Correia, Yulli Roxenne Albuquerque, Bruna Dias de Lima Fragelli, Tiago Manuel Fernandes Mendes, Silmara Marques Allegretti, Edson Garcia Soares, Larissa Romanello, Juliana Roberta Torini, Humberto D'Muniz Pereira, Fernanda de Freitas Anibal

2615 related Products with: Investigating Immunization With Nucleotide Enzymes of : Nucleoside Diphosphate Kinase and Adenylosuccinate Lyase as New Antigenic Targets Against Schistosomiasis.

100ug Lyophilized400Tests100ug Lyophilized100ug Lyophilized100ug Lyophilized1x96 well plate1 kit100ug Lyophilized400100ug Lyophilized100ug Lyophilized100ug Lyophilized

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#33071180   2020/09/25 To Up

Prophylactic vaccination with a live-attenuated herpes zoster vaccine in lung transplant candidates.

Herpes zoster (HZ) is caused by the reactivation of varicella-zoster virus (VZV). Patients with lung transplants are at high risk for HZ owing to their immunocompromised status and the need for lifelong immunosuppression. In this study, patients on the waiting list for lung transplantation were vaccinated by a live-attenuated HZ vaccine (Zostavax, Merck Sharp & Dohme), and the safety and immunogenicity of this vaccine were studied.
Lei Wang, Erik A M Verschuuren, Davy Paap, Christien Rondaan, Elisabeth Raveling-Eelsing, Johanna Westra, Nicolaas A Bos

2670 related Products with: Prophylactic vaccination with a live-attenuated herpes zoster vaccine in lung transplant candidates.



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#33070876   2020/08/05 To Up

Pruritus in neuromyelitis optica spectrum disorders and multiple sclerosis.

Differential diagnosis between neuromyelitis optica spectrum disorders (NMOSD) and multiple sclerosis (MS) at early stage remains challenging at present. Pruritus is reported as a common or specific feature in NMOSD with serum aquaporin-4 immunoglobulin G antibodies (AQP4-IgG). We aim to verify whether pruritus can help in distinguishing NMOSD from MS.
Mianwang He, Lei Wu, Dehui Huang, Vicky Yau, Shengyuan Yu

1211 related Products with: Pruritus in neuromyelitis optica spectrum disorders and multiple sclerosis.

50 ul5mg50 ul

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#33069413   2020/10/14 To Up

Feeding colostrum or a 1:1 colostrum:whole milk mixture for 3 days after birth increases serum immunoglobulin G and apparent immunoglobulin G persistency in Holstein bulls.

Conflicting reports exist on whether prolonged IgG consumption can further increase serum IgG in neonatal calves. Given that higher serum IgG in neonates has lifelong benefits, our objective was to determine whether serum IgG can be increased by providing multiple meals containing IgG to neonatal calves. Twenty-seven Holstein bulls were all fed 1 colostrum meal (7.5% body weight; 62 g of IgG/L) at 2 h after birth and randomly assigned to be fed (5% body weight) colostrum (COL; n = 9), whole milk (WM; n = 9), or a 1:1 colostrum:whole milk mixture (MX; n = 9) every 12 h from 12 to 72 h. Serum IgG was measured at 1, 2, 3, 6, 9, 11, and 12 h after birth. After the 12-h meal, IgG was determined at 0.5-h intervals until 16 h and then at 1-h intervals from 16 to 24 h. Serum IgG was then measured at 27 h, then every 6 h from 30 to 60 h. From 60 to 64 h, IgG was measured every 0.5 h, then at 65 and 66 h, and then every 2 h until 72 h. Serum IgG increased rapidly between 2 and 12 h for all calves. A treatment × time interaction occurred as serum IgG began to diverge between treatments after they were fed at 12 h; the interaction was greatest over the entire period for COL compared with both MX and WM and was greater for MX than for WM. Maximum IgG concentrations (C) were 30.4 ± 0.8, 27.2 ± 0.8, and 23.9 ± 0.8 g/L for COL, MX, and WM, respectively. Although MX C was equivalent to both COL and WM C, COL C was greater than WM C. Feeding COL and MX also prolonged the time to reach C. Respectively, these calves achieved C at 29.5 and 27.0 ± 3.4 h, whereas WM IgG peaked at 13.4 ± 3.4 h. No differences were observed for apparent efficiency of absorption between treatments from 0 to 12 h and 0 to 24 h. Immunoglobulin G area under the curve (AUC) was the same for COL and MX calves over the entire experimental period and from when treatments were fed. The IgG AUC for 0 to 72 h for WM calves was 27.4% lesser than that for COL calves but not different from MX calves. However, the IgG AUC for 12 to 72 h for WM calves differed relative to that for both COL (30.8% less) and MX (19.6% less) calves. Serum IgG concentrations were more persistent when COL (88.2 ± 2.4%) and MX (91.2 ± 2.4%) were fed rather than WM (75.3 ± 2.4%). Prolonged IgG consumption increased serum IgG concentrations, corresponding to the mass of IgG fed, and improved apparent IgG persistency in Holstein bulls. Neonatal calves should be fed at least 62 g of IgG at 12 h after birth to further increase serum IgG concentrations.
K S Hare, S Pletts, J Pyo, D Haines, L L Guan, M Steele

1055 related Products with: Feeding colostrum or a 1:1 colostrum:whole milk mixture for 3 days after birth increases serum immunoglobulin G and apparent immunoglobulin G persistency in Holstein bulls.

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#33067924   2020/10/17 To Up

CD200-CD200R1 signaling pathway regulates neuroinflammation after stroke.

To study how the CD200-CD200R1 signaling pathway modulates poststroke inflammation and advances our knowledge of immune responses to ischemia insults in stroke.
Shou-Cai Zhao, Xu Heng, Wang Ya-Ping, Luan Di, Wu Wen-Qian, Ma Ling-Song, Zhao-Hu Chu, Yang Xu

2299 related Products with: CD200-CD200R1 signaling pathway regulates neuroinflammation after stroke.

2 Pieces/Box1 mg2 Pieces/Box2 Pieces/BoxInhibitors1.5x10(6) cells2 Pieces/Box8 inhibitors2 Pieces/Box14 inhibitors2 Pieces/Box2 Pieces/Box

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#33066057   2020/10/13 To Up

Kinetics of Nucleo- and Spike Protein-Specific Immunoglobulin G and of Virus-Neutralizing Antibodies after SARS-CoV-2 Infection.

Kinetics of neutralizing antibodies and immunoglobulin G (IgG) against the nucleo (N) or spike (S) proteins of (SARS-CoV-2) were studied in patients up to 165 days after PCR diagnosis of infection. Two immunoassays were selected out of eight IgG or total antibody tests by comparing their specificities and sensitivities. Sensitivities were calculated with convalescent sera from 26 PCR-confirmed cases, of which 76.9% had neutralizing antibodies (>1:10). Stored sera collected during the summer 2018 (N = 50) and winter seasons 2018/2019 (N = 50) were included to demonstrate the test specificities. IgG kinetics, avidities, and virus-neutralizing capacities were recorded over up to 165 days in eleven patients and five individuals from routine diagnostics. Sensitivities, specificities, and diagnostic accuracies ranged between 80.8-96.3%, 96.0-100%, and 93.7-99.2%, respectively. Nearly all results were confirmed with two different SARS-CoV-2-specific immunoblots. Six (54.4%) patients exhibited stable N-specific IgG indices over 120 days and longer; three of them developed IgG of high avidity. The S-specific IgG response was stable in ten (91.0%) patients, and eight (72.7%) had neutralizing antibodies. However, the titers were relatively low, suggesting that sustained humoral immunity is uncertain, especially after outpatient SARS-CoV-2 infection.
Annabelle Strömer, Ruben Rose, Olaf Grobe, Franziska Neumann, Helmut Fickenscher, Thomas Lorentz, Andi Krumbholz

1814 related Products with: Kinetics of Nucleo- and Spike Protein-Specific Immunoglobulin G and of Virus-Neutralizing Antibodies after SARS-CoV-2 Infection.

20 ug Product tipe: Antib100 100 100 100 100.00 ug0.1 mg1000 TESTS/0.65ml0.1 mg100 100100

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#33065988   2020/10/13 To Up

Comparison of Different HILIC Stationary Phases in the Separation of Hemopexin and Immunoglobulin G Glycopeptides and Their Isomers.

Protein glycosylation analysis is challenging due to the structural variety of complex conjugates. However, chromatographically separating glycans attached to tryptic peptides enables their site-specific characterization. For this purpose, we have shown the importance of selecting a suitable hydrophilic interaction liquid chromatography (HILIC) stationary phase in the separation of glycopeptides and their isomers. Three different HILIC stationary phases, i.e., HALO penta-HILIC, Glycan ethylene bridged hybrid (BEH) Amide, and ZIC-HILIC, were compared in the separation of complex -glycopeptides of hemopexin and Immunoglobulin G glycoproteins. The retention time increased with the polarity of the glycans attached to the same peptide backbone in all HILIC columns tested in this study, except for the ZIC-HILIC column when adding sialic acid to the glycan moiety, which caused electrostatic repulsion with the negatively charged sulfobetaine functional group, thereby decreasing retention. The HALO penta-HILIC column provided the best separation results, and the ZIC-HILIC column the worst. Moreover, we showed the potential of these HILIC columns for the isomeric separation of fucosylated and sialylated glycoforms. Therefore, HILIC is a useful tool for the comprehensive characterization of glycoproteins and their isomers.
Katarina Molnarova, Petr Kozlík

2560 related Products with: Comparison of Different HILIC Stationary Phases in the Separation of Hemopexin and Immunoglobulin G Glycopeptides and Their Isomers.

1000 tests100 μg0.1 mg96 wells (1 kit)

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