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#33091948   2020/10/22 To Up

Challenges in Detecting Clinically Relevant Heparin-Induced Thrombocytopenia Antibodies.

Heparin-induced thrombocytopenia (HIT) is an antibody-mediated hypercoagulable state featuring high thrombosis risk and distinct pathogenesis involving immunoglobulin G-mediated platelet activation. The target of the immune response is a cationic "self" protein, platelet factor 4 (PF4), rendered antigenic by heparin. A key problem is that only a minority of anti-PF4/polyanion antibodies induced by heparin are pathogenic, i.e., capable of causing platelet activation and thereby clinical HIT. Since thrombocytopenia occurs frequently in hospitalized, heparin-treated patients, testing for "HIT antibodies" is common; thus, the problem of distinguishing between pathogenic and nonpathogenic antibodies is important. The central concept is that those antibodies that have platelet-activating properties demonstrable in vitro correlate well with pathogenicity, as shown by platelet activation tests such as the serotonin-release assay (SRA) and heparin-induced platelet activation assay. However, in most circumstances, immunoassays are used for first-line testing, and so it is important for clinicians to appreciate which immunoassay result profiles-in the appropriate clinical context-predict the presence of platelet-activating antibodies (Bayesian analysis). Clinicians with access to rapid, on-demand HIT immunoassays (e.g., particle gel immunoassay, latex immunoturbidimetric assay, chemiluminescent immunoassay) can look beyond simple dichotomous result interpretation ("negative"/"positive") and incorporate semiquantitative interpretation, where, for example, a strong-positive immunoassay result (or even combination of two immunoassays) points to a greater probability of detecting platelet-activating antibodies, and hence supporting a diagnosis of HIT. Recent recognition of "SRA-negative HIT" has increased the importance of semiquantitative interpretation of immunoassays, given that strong immunoassay reactivity is a potential clue indicating possible HIT despite a (false) negative platelet activation assay.
Theodore E Warkentin

2896 related Products with: Challenges in Detecting Clinically Relevant Heparin-Induced Thrombocytopenia Antibodies.

100 μg100 μg100 μg4 Membranes/Box100.00 ug100.00 ug100 μg20 100 μg4 Arrays/Slide100 μg100 μg

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#33091925   2020/10/22 To Up

Short communication: maternal melatonin implants improve twin Merino lamb survival.

High pre-weaning mortality rates cost the Australian sheep industry an estimated $540 million annually in lost production, with losses significantly greater in twin (≥ 30%) compared with singleton lambs (≥ 10%). Previous intensive studies demonstrated that supplementing pregnant ewes with melatonin reduces adverse effects of fetal growth restriction and perinatal hypoxia on the neonatal brain via increased umbilical blood flow, placental efficiency, and antioxidant actions. The current study examined the effects of supplementing ewes with melatonin on the survival of twin Merino lambs under extensive grazing conditions. Pregnant mixed age ewes were implanted with 1 (M1, n = 50) or 2 (M2, n = 53) slow-release melatonin implants (18 mg, Regulin) at gestational d 70-90. Control ewes received no supplementation (CTL, n = 54). Ewes were monitored twice daily throughout the lambing period. Lamb survival, weight, and rectal temperature were recorded on the day of birth. Lamb blood samples were taken the following day for serum immunoglobulin G (IgG) analysis. Lamb survival and weight were recorded again at marking (30.6 ± 0.6 d post-partum) and weaning (70.7 ± 0.6 d post-partum). Lamb survival was increased in both melatonin treatments to 3 d post-partum (M1 = 98.0%; M2 = 95.3%; CTL = 83.3%; each P < 0.01) and this improvement was maintained to weaning (M1 = 94.0%; M2 = 92.5%; CTL = 79.6%; each P < 0.01). Melatonin did not affect lamb birthweight, rectal temperature, or growth rate. However, the rates of parturition-related death (dystocia, stillbirth, birth injury) were greater in CTL lambs than M1 (P = 0.009) and M2 (P = 0.035). This suggests that improved survival is primarily due to melatonin-induced neuroprotection, although further studies are required to clarify the underlying mechanisms. These data provide evidence that supplementing pregnant twin-bearing Merino ewes with melatonin may be a practical strategy to reduce neonatal mortality and improve weaning rates in extensively managed sheep flocks. Although the present data are promising, this study is limited by small sample size and requires further replication.
Tom Flinn, Jessica R Gunn, Karen L Kind, Alyce M Swinbourne, Alice C Weaver, Jennifer M Kelly, Simon K Walker, Kathryn L Gatford, William H E J van Wettere, David O Kleemann

2012 related Products with: Short communication: maternal melatonin implants improve twin Merino lamb survival.

1 ml1mg0.1 mg500 ul (0.2 ug/ul) 6 ml Ready-to-use 1 mg25 μg0,25ml / 50 test100 5 X 20,000 U1 mg

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#33091685   2020/10/12 To Up

An origami electrical biosensor for multiplexed analyte detection in body fluids.

We developed an affordable, highly sensitive, and specific paper-based microfluidic platform for fast multiplexed detections of important biomarkers in various body fluids, including urine, saliva, serum, and whole blood. The sensor array consisted of five individual sensing channels with various functionalities that only required a micro liter-sized sample, which was equally split into aliquots by the built-in paper microfluidics. We achieved the individual functionalizations of various bioreceptors by employing the use of wax barriers and 'paper bridges' in an easy and low-cost manner. Pyrene carboxylic acid-modified single-walled carbon nanotubes (PCA/SWNTs) were deposited by quantitative inkjet printing with an optimal 3-dimensional semiconductor density on a paper substrate. Multiple antibodies were immobilized onto the SWNTs surface for highly sensitive and specific field-effect transistor (FET)/chemiresistor (CR) biosensors. We explored the optimal sensing conditions for the paper-based CR biosensor to achieve high sensitivities and specificities towards the target biomarker proteins (human serum albumin (HSA) and human immunoglobulin G (HIgG)) and achieved an ultralow detectable concentration of HSA and HIgG at 1.5 pM. Besides, origami folding was employed to simplify the fabrication process further. The sensing platform described in this work was cost-effective, semi-automated, and user-friendly. It demonstrated the capability of having multiple sensing functions in one paper-based microfluidic sensing platform. It envisioned the potential of a point-of-care device with full-analysis for practical diagnostics in an ASSURED (Affordable, Sensitive, Specific, User-friendly, Rapid and robust, Equipment-free and Deliverable to end-users) fashion for a quick test of targets of interest.
Yu Shen, Sidharth Modha, Hideaki Tsutsui, Ashok Mulchandani

2214 related Products with: An origami electrical biosensor for multiplexed analyte detection in body fluids.

100 μg100 μg100 μg100 μg1 Set100 μg 100ul100.00 ug1 Set1 Set

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#33091678   2020/10/06 To Up

Purification of Fab and Fc using papain immobilized cryogel bioreactor separator system.

The continuous bed bioreactor systems have been used for the production of protein therapeutics, such as IgG, using immobilized enzyme in biopharmaceutical applications. We developed macroporous poly(hydroxyethyl methacrylate-co-glycidyl methacrylate) cryogel-based bioreactor matrix using sodium dodecyl sulfate as surfactans in the presence of ethylene glycol dimethacrylate as cross linking agent by bulk polymerization. The developed polyGMA immobilized bioreactor with papain enzyme was used for specific fragmentation of immunoglobulin G. The catalysis efficiency for immobilized enzyme were investigated in comparison with free enzyme. The immobilized papain displayed broad catalytic activity over a variety of conditions, with maximal activity around pH 7.0 and 70 °C. The Michaelis-Menten kinetic constant (K), the maximum reaction velocity (V), and the catalytic efficiency (k) for free enzyme were 0.1097 mg/mL, 29.9 mg/mL/min, and 92.01 1/min, respectively, whereas for immobilized enzyme, K, V, and k values were 0.1078 mg/mL, 30.53 mg/mL/min, and 94.3 1/min, respectively. In a further step, after digestion, remarkable digestion products of bioreactor, Fab and Fc fragments, produced with immobilized papain bioreactors were analyzed in two ways by SDS-PAGE and reversed-phase HPLC; it was demonstrated that papain immobilized bioreactor successfully used for the digestion of human IgG with high activity. Therefore, the polyGMA cryogel immobilized with papain exhibited a very effective matrix for the bioreactor which can be considered as an alternative bioreactor matrix with great promise in biopharmaceutical applications.
Canan Armutcu, Mehmet Emin Çorman, Engin Bayram, Lokman Uzun

1797 related Products with: Purification of Fab and Fc using papain immobilized cryogel bioreactor separator system.

5ml100ug Lyophilized 500 Slides 110 mg5kits25 ìg10ml1 kit(96 Wells) 70 Slides 100ug1000 tests

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#33091579   2020/10/19 To Up

Effects of Toxoplasma gondii infection and schizophrenia comorbidity on serum lipid profile: A population retrospective study from Eastern China.

Toxoplasma gondii (T. gondii), a parasitic protozoa that is associated with various psychiatric disorders. Both T. gondii infection and disturbed host's lipid profile are common in schizophrenia patients. However, the underlying pathophysiological mechanisms remain speculative. Also, the characteristics of serum lipid levels in schizophrenia patients comorbid with T. gondii infection are not clear. Therefore, it is necessary to explore the influence of chronic T. gondii infection on the characteristic physiological indexes of schizophrenia patients so as to provide some insights into finding target therapeutic drugs.
Fei Xu, Xinyu Ma, Yuwei Zhu, Arjen Sutterland, Ruitang Cheng, Sunhan Miao, Jialu Chen, Liying Qiu, Yonghua Zhou

2017 related Products with: Effects of Toxoplasma gondii infection and schizophrenia comorbidity on serum lipid profile: A population retrospective study from Eastern China.

100 µg100 µg1 mg100 µg100 100ug/vial1,000 tests96T100ug10 mg1 ml2ml

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#33090535   2020/10/22 To Up

Possible vertical transmission and antibodies against SARS-CoV-2 among infants born to mothers with COVID-19: A living systematic review.

Current evidence suggests that coronavirus disease 2019 (COVID-19), caused by severe respiratory syndrome coronavirus 2 (SARS-CoV-2), is predominantly transmitted from human-to-human. However, evidence on vertical transmission and natural passive immunity among the newborns exposed to COVID-19 is scanty and varies. This poses a challenge on preventive interventions for the newborns. We conducted a systematic review to first, determine the likelihood of vertical transmission among COVID-19 exposed infants and second, determine whether antibodies against SARS-CoV-2 were generated among COVID-19 vertically exposed but negative infants. This review registered in PROSPERO searched evidence from PubMed/ MEDLINE and Google Scholar, among others. About 517 studies were pooled, where 33 articles (5.8%) met the inclusion criteria such as infection prevention and control measures at birth. A total of 205 infants born to COVID-19 positive mothers were studied. Overall, 6.3% (13/205; 95%CI: 3.0%-9.7%) of the infants tested positive for COVID-19 virus at birth. Of 33 eligible studies, 6 studies (18.8%) reported about immunoglobulin G/ M (IgG/IgM) against SARS-CoV-2. IgG/IgM were detected in 90% infants (10/11; 95%CI: 73.9%-107.9%) who tested negative for COVID-19 virus. The median antibody levels detected were 75.49AU/mL (range: 7.25AU/mL-140.32AU/mL) and 3.79AU/mL (range: 0.16AU/mL-45.83AU/mL), p = 0.0041 for IgG and IgM, respectively. In conclusion, the current evidence revealed a low possibility of vertical transmission of COVID-19 and antibodies against SARS-CoV-2 were detected among vertically exposed but negative infants. Further studies on transplacental transmission and the magnitude of natural passive immunity in infants born to mothers with COVID-19 are warranted. This article is protected by copyright. All rights reserved.
George M Bwire, Belinda J Njiro, Dorkasi L Mwakawanga, Deodatus Sabas, Bruno F Sunguya

1969 related Products with: Possible vertical transmission and antibodies against SARS-CoV-2 among infants born to mothers with COVID-19: A living systematic review.

200ug100 200 ug0.1 mg50 ug Product tipe: Antib0.5 ml0.1 mg200ug0.1 ml100 1 mL100

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#33089226   2020/09/30 To Up

Influences of dietary flavonoid (quercetin) supplementation on growth performance and immune response of growing pigs challenged with lipopolysaccharide.

This study was conducted to evaluate the effects of dietary supplementation of plant flavonoid (quercetin) on immune parameters, growth performance, and nutrient digestibility in growing pigs challenged with lipopolysaccharide (LPS). A total of 40 crossbred ([Landrace × Yorkshire] × Duroc) growing pigs; initial body weight (BW) of 26.95 ± 1.26 kg were used in a six-week experimental trial. Pigs were randomly allocated into one of four treatment groups in a 2 × 2 factorial arrangement with the following factors; without LPS challenge and with LPS challenge (day 21) supplemented with or without 0.1% flavonoid according to BW (2 replicate pens per treatment with 2 gilts and 3 barrows per pen). The single- dose LPS (100 ug / kg BW) injection showed trends tended to be increased in interleukin-6 (IL-6) after 2 h and 6 h of challenge compared with unchallenged pigs. However, other measured immune indices (white blood cell, immunoglobulin G, lymphocyte, and tumor necrosis factor), growth performance, and nutrient digestibility were not significantly different between challenged and non-challenged animals. The supplementation of flavonoid significantly increased ( < 0.05) average daily gain (ADG) during day 0-21, tended to increase dry matter and nitrogen digestibility, significantly reduced IL-6, increased Ig-G and WBC concentrations and increased lymphocytes percentage regardless of LPS challenge.
Jae-Hong Park, Shanmugam Sureshkumar, In Ho Kim

2551 related Products with: Influences of dietary flavonoid (quercetin) supplementation on growth performance and immune response of growing pigs challenged with lipopolysaccharide.

5 G2ug20 ug 6 ml Ready-to-use 2 ml10 mg5 per Sleeve, 100 Flasks/100ul2ug10 μg

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#33088532   2020/08/07 To Up

Immune Thrombocytopenic Purpura, a rare manifestation of active tuberculosis: Case report.

Tuberculosis is currently considered a public health issue worldwide. Although a large number of hematological disorders related to tuberculosis have been described, immune thrombocytopenic purpura in patients with active tuberculosis is very rare but must be considered in a patient with no other evident cause.
Yadhira A Fajardo, Eliana I Morales, Luz F Sua, Liliana Fernández-Trujillo

1212 related Products with: Immune Thrombocytopenic Purpura, a rare manifestation of active tuberculosis: Case report.

100 units

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#33088501   2020/10/16 To Up

Modulations of genes related to gut integrity, apoptosis, and immunity underlie the beneficial effects of CECT 5940 in broilers fed diets with different protein levels in a necrotic enteritis challenge model.

The ban of in-feed antimicrobial additives has negatively affected the poultry industry by causing necrotic enteritis (NE) to emerge in the flocks. Alternatives such as probiotics have shown to be effective on eliminating the negative effects of this disease. Two experiments were conducted to investigate the effect of CECT 5940 (BA) in broiler chickens under NE challenge and/or fed diets with different protein levels.
Kosar Gharib-Naseri, Juliano Cesar de Paula Dorigam, Kiran Doranalli, Sarbast Kheravii, Robert A Swick, Mingan Choct, Shu-Biao Wu

2454 related Products with: Modulations of genes related to gut integrity, apoptosis, and immunity underlie the beneficial effects of CECT 5940 in broilers fed diets with different protein levels in a necrotic enteritis challenge model.

2 Pieces/Box2 Pieces/Box100 100 ug1 mg0.1 mg100 ug100 100 μg

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#33086397   2020/10/21 To Up

Characterization of New Monoclonal PF4-Specific Antibodies as Useful Tools for Studies on Typical and Autoimmune Heparin-Induced Thrombocytopenia.

 Heparin-induced thrombocytopenia (HIT) is typically caused by platelet-activating immunoglobulin G (IgG) antibodies (Abs) against platelet factor 4 (PF4) complexed with heparin (H). Much less frequent "autoimmune" HIT is distinguished from typical HIT by platelet activation without heparin and the presence of both anti-PF4/H and anti-PF4 IgG. We developed three murine monoclonal anti-PF4 Abs with a human Fc-part, 1E12, 1C12, and 2E1, resembling autoimmune HIT Abs.
Caroline Vayne, Thi-Huong Nguyen, Jérôme Rollin, Noémie Charuel, Anne Poupon, Claire Pouplard, Nicole Normann, Yves Gruel, Andreas Greinacher

1155 related Products with: Characterization of New Monoclonal PF4-Specific Antibodies as Useful Tools for Studies on Typical and Autoimmune Heparin-Induced Thrombocytopenia.

25 µg100.00 ug100 TESTS200 ug0.2 mg0.2 mg1 ml100.00 ug25 µg100.00 ug0.25 mg100.00 ug

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