Product name :Glutathione Reductase Assay Kit

Catalog Number :K761-200

Quantity :200 assays

Price :357.21 €

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Supplier :Biovision


Glutathione Reductase Activity Assay Kit
(Catalog #K761-200; 200 reactions;
Store kit at –20°C)


I. Introduction:


Glutathione Reductase (GR, EC 1.8.1.7) catalyzes the NADPH-dependent reduction of oxidized glutathione (GSSG) to reduced glutathione (GSH), which plays an important role in the GSH redox cycle that maintains adequate levels of reduced GSH. A high GSH/GSSG ratio is essential for protection against oxidative stress. BioVision’s Glutathione Reductase Assay Kit is a highly sensitive, simple, direct and HTS-ready colorimetric assay for measuring GR activity in biological samples. In the assay, GR reduces GSSG to GSH, which reacts with 5, 5′- Dithiobis (2-nitrobenzoic acid) (DTNB) to generate TNB2- (yellow color, λmax = 405 nm). The assay can detect 0.1 – 40 mU/ml GR in various samples.


II. Kit Contents:

• GR Assay Buffer
• 3% H₂O₂
• Catalase  
• TNB Standard (2.5 µmol)
• DTNB 
• NADPH-GNERAT™
• GSSG
• GR Positive Control (10 mU)


III. Storage and Handling:


Store kit at -20°C, protect from light. Warm Assay Buffer to room temperature before use.
Briefly centrifuge vials before opening. Read the entire protocol before performing the assay.


IV. Reagent Reconstitution and General Consideration:


Catalase: Dissolve lyophilized catalase with 1 ml Assay Buffer. The Catalase solution is stable for 1 week at 4°C and 1 month at -20°C.
TNB Standard: Dissolve lyophilized TNB standard with 0.5 ml Assay Buffer to generate 5 mM TNB Standard. The TNB standard solution is stable for 1 week at 4 °C and 1 month at -20°C.
DTNB Solution: Dissolve DTNB with 0.45 ml Assay Buffer, sufficient for 200 assays. The
DTNB solution is stable for 2 weeks at 4°C and 1 month at -20°C.
NADPH-GNERAT™: Dissolve one vial with 0.22 ml Assay Buffer; sufficient for 100 assays. The solution is stable for 10 hours at 4°C and 2 weeks at -20°C.
GSSG: Dissolve GSSG with 1.3 ml Assay Buffer, sufficient for 200 assays. The GSSG solution is stable for 2 weeks at 4°C and 2 months at -20°C.
GR Positive Control: Dissolve lyophilized GR into 100 μl Assay Buffer, aliquot into vials, store at -20°C. It is stable for 1 day at 4°C and 1 month at -20°C.
Ensure that the Assay Buffer is at room temperature before use. Keep samples, NADPHGNERAT ™ solution and GR standard on ice during the assay.
V. Glutathione Reductase Activity Assay:
1. Sample Preparations: Homogenize 0.1 gram tissues, or 1 x 106 Cells, or 0.2 ml Erythrocytes on ice in 0.5 - 1.0 ml cold assay buffer; Centrifuge at 10,000 x g for 15 min at 4°C; Collect the supernatant for assay and store on ice, serum can be tested directly. Store at -80°C.
2. Sample Pretreatment: Samples should be treated to destroy GSH before the assay. Take
100 μl sample, add 5 μl 3% H2O2, mix and incubate at 25°C for 5 min. Then add 5 μl of catalase, mix and incubate at 25°C for another 5 min. Add 2 -50 μl of the pretreated samples
into a 96-well plate, bring the volume to 50 μl with Assay Buffer. We suggest testing several
doses of your sample to make sure the readings are within the standard curve range. Use 10
μl /well Positive Control (optional) and adjust to 50 μl with Assay Buffer.
3. TNB Standard Curve:
Add 0, 2, 4, 6, 8, 10 μl of the TNB Standard into 96-well plate in duplicate to generate 0, 10,
20, 30, 40, 50 nmol/well standard. Bring the final volume to 100 μl with Assay Buffer.
4. Reaction Mix: Mix enough reagents for the number of assays to be performed. For each well, prepare a total 50 μl Reaction Mix:
40 μl GR Assay Buffer
2 μl DTNB solution
2 μl NADPH-GNERAT™ solution
6 μl GSSG solution
Add 50 μl of the Reaction Mix to each test samples. Mix well. Measure OD 405 nm at T1 (reading A1). Incubate the reaction at 25°C for 10 min (or incubate longer time if the GR activity is low), protect from light, measure O.D.405 nm again at T2 (reading A2). ΔA405 nm = A2 – A1.
Note: It is essential to read A1 and A2 in the reaction linear range. It will be more accurate if
you read the reaction kinetics, and ensure A1 and A2 in the reaction linear range.
5. Calculation: Plot the TNB standard Curve. Apply the ΔA405nm to the TNB standard curve to get ΔB nmol of TNB (TNB amount generated between T1 and T2 in the reaction wells).
Where: ΔB is the TNB amount from TNB standard Curve (in nmol).
T1 is the time of the first reading (A1) (in min).
T2 is the time of the second reading (A2) (in min).
V is the pretreated sample volume added into the reaction well (in ml).
0.9 is the sample volume change factor during sample pre-treatment procedure.
Unit Definition: One unit is defined as the amount of enzyme that generates 1.0 μmol of TNB per minute at 25°C. The oxidation of 1 mole of NADPH to NADP+ will generate 2 mole TNB finally, therefore, 1 TNB unit equals 0.5 NADP unit.


VI. Related Products:


Colorimetric Glutathione Detection Kit
ApoGSH Glutathione Detection Kit
Glutathione Kit (GSH, GSSG and Total) GST Fluorometric Assay Kit
GST Colorimetric Assay Kit
Triglyceride Assay Kit
Acid Phosphatase Assay Kit
ADP/ATP Ratio Assay Kit
Phosphate Fluorescence Assay Kit
Phosphate Colorimetric Assay Kit
NAD/NADH Quantification Kit
NADP/NADPH Quantitation Kit
Pyruvate Assay Kit
Lactate Assay Kit/ II
Ammonia Assay Kit
Glutamate Assay Kit
Glucose Assay Kit
Fatty Acid Assay Kit
Ethanol Assay Kit
Uric Acid Assay Kit
 

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K317-100 PicoProbe™Acetyl CoA Assay Kit Biovision 100 assays 351.50€ Pub
LF-MA0020 anti-Thioredoxin Reductase 1 (5A5), Mouse monoclonal to Thioredoxin Reductase 1, Isotype IgG1, Host Mouse Abfrontier 100 ul 355.00€ Pub
1002-1000 Annexin V Cy3 Reagent Biovision 1000 assays 945.50€
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Pub
K109-25 Annexin V Biotin Apoptosis Kit Biovision 25 assays 162.50€
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Pub
  P-4012-96  EpiQuik Global Acetyl Histone H3K14 Quantification Kit (Colorimetric) (96 assays) EpigenTek 618.00€
ASK
Pub
1043 Human monkey anti-porcine type I collagen IgA antibody assay kit, OPD Chondrex 528.36€
ASK
Pub
2045T Rat anti-human type II collagen IgG antibody assay kit, TMB Chondrex 434.70€
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Pub
52005 Methyltransferase PRMT3 Assay Kit BPS Bioscience 100 reactions 530.00€
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Pub
ECPK-100 EnzyChrom™ Creatine Kinase Assay Kit Bioassays 100 325.00€ Pub
K125-25 Caspase 10 Colorimetric Assay Kit Biovision 25 assays 180.50€
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Pub
K745-100 Myeloperoxidase (MPO) Fluorometric Activity Assay Kit Biovision 100 assays 406.00€ Pub
P-3016-096  EpiQuik In Situ Histone H3K9 Methylation Assay Kit (96 assays) EpigenTek 539.00€ Pub
1109-200 Caspase 4 Substrate LEVD AFC200 assays Biovision 200 assays 276.89€
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Pub
K790-10000 StayBrite™ Highly Stable Luciferase Luciferin Reagent Biovision 10000 assays 4 876.00€ Pub
20332T Mouse anti-porcine type II collagen IgG2a antibody subtype assay kit, TMB Chondrex 400.05€
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36006 Screen Quest™ Membrane Potential Assay Kit *Red Fluorescence* AAT Bioquest 100 plates 3 131.00€
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Pub
DIMG-250 QuantiChrom™ Magnesium Assay Kit Bioassays 250 331.00€ Pub
K111-25 Caspase 1 Colorimetric Assay Kit Biovision 25 assays 162.50€
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Pub
K624-100 Lactose Assay Kit Biovision 100 assays 357.00€ Pub
M1193 MarkerGeneTM Gaussia Luciferase Cellular Assay Kit, Utilizes a new luciferase which does not require ATP and can be expressed in mammalian cells using commercially available reporter plasmids, 1 kit MarkerGene 140.00€
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Pub
1072-1000 Caspase-2 Substrate VDVAD-pNA; Appearance Liquid Biovision 1000 assays 635.04€
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Pub
K200-100 CaspSCREEN Caspase Screening Kit Biovision 100 assays 521.00€
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Pub
20334T Mouse anti-porcine type II collagen IgG3 antibody subtype assay kit, TMB Chondrex 400.05€
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Pub
36309 Screen Quest™ Fluo 8 Medium Removal Calcium Assay Kit *Optimized for Difficult Cell Lines* AAT Bioquest 100 Plates 1 571.00€
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Pub
DIUR-500 QuantiChrom™ Urea Assay Kit, Quantitative determination of urea by chemical colorimetric (520nm) method Bioassays 500Tests 300.00€ Pub
K112-400 Caspase 8 Fluorometric Assay Kit Biovision 400 assays 702.50€
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Pub
K629-100 Glutamate Assay Kit Biovision 100 assays 377.00€ Pub
M12486111 L Glutathione oxidised CAS Number [27025 41 8] Molekula 5 G 151.00€
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Pub
1072-200 Caspase 2 Substrate VDVAD pNA200 assays Biovision 200 assays 287.28€
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Pub
K202-100 Annexin V Cy3 Apoptosis Kit Plus Biovision 100 assays 333.00€
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Pub
20100 Cell Meter™ Live Cell Caspase 3 7 Binding Assay Kit *Green Fluorescence* AAT Bioquest 100 tests 212.00€
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Pub
22836 Cell Meter™ Phosphatidylserine Apoptosis Assay Kit *Green Fluorescence Excited at 405 nm* AAT Bioquest 1 kit 202.00€
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Pub
IHVNKT-TOT Human Vitronectin Total Antigen Assay Innovative Research INC 1 kit(96 Wells) 597.00€ Pub
K390-100 Iron Assay Kit Biovision 100 assays 377.00€ Pub
1008-200 Caspase 3 Substrate DEVD pNA200 assays Biovision 200 assays 276.00€
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Pub
K154-100 Caspase 4 Inhibitor Drug Screening Kit Biovision 100 assays 387.50€
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Pub
  P-4023-48  EpiQuik Global Acetyl Histone H4K5 Quantification Kit (Fluorometric) (48 assays) EpigenTek 358.00€
ASK
Pub
11559 Amplite™ Luminometric Peroxidase Assay Kit AAT Bioquest 1 kit 202.00€
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Pub
22-553W 96-Well Assay Plates V Bottom, White Genesee 10 Plates/Unit 80.00€
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Pub
EGTT-100 EnzyChrom™ GSH GSSG Assay Kit Bioassays 100 tests 221.00€ Pub
K252-200 Nitric Oxide Fluorometric Assay Kit Biovision 2 x 96 assays 431.00€
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Pub
K787-100 Alcohol Dehydrogenase Activity Assay Kit Biovision 100 assays 386.00€ Pub
SL100489-CaS Transfection Reagents and Kits, GenJet™ DNA In Vitro Tranfection Reagent for Ca Ski was pre optimized for most efficiently transfecting Ca Ski cells. Signagen 1 ml 231.37€ Pub
1116-200 Caspase-13 Substrate LEED-AFC; Appearance Liquid Biovision 200 assays 311.85€
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Pub
P-1014B-96  Methylamp Global DNA Methylation Quantification Ultra Kit (96 assays) EpigenTek 808.50€
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Pub
1012 Mouse anti-bovine type I collagen IgG antibody assay kit, OPD Chondrex 373.80€
ASK
Pub
20355 Mouse anti-human type II collagen IgG2c antibody subtype assay kit, OPD Chondrex 442.05€
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Pub
36374 Screen Quest™ Fluorimetric ELISA cAMP Assay Kit AAT Bioquest 10 plates 1 571.00€
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Pub
E2ND-100 EnzyChrom™ NAD NADH Assay Kit, Sensitive determination of NAD and NADH by colorimetric (565nm) method. Procedure 15 min. Kit size 100 tests. Detection limit 0.05 µM. Shelf life 6 months. Shipping Bioassays 100tests 364.00€ Pub
K116-400 Caspase 2 Fluorometric Assay Kit Biovision 400 assays 738.50€
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Pub
K657-100 Glucose 6 Phosphate Assay Kit Biovision 100 assays 351.50€ Pub
MA-0201 siRNA Plate Assay Signosis 2011 96 595.00€ Pub
1101-1000 Caspase 5 Substrate WEHD AFC1000 assays Biovision 1000 assays 541.49€
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Pub
K267-50 Cytosol/Particulate Separation Kit Biovision 50 assays 322.25€ Pub
20313 Mouse anti-chick type II collagen IgG2b antibody subtype assay kit, OPD Chondrex 400.05€
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Pub
298-65701 Glucose Assay With the LabAssay Glucose Assay, when a sample is mixed with the Choromogen Reagent, the alpha form of glucose in the sample is converted to beta form by mutarotase. beta DGlucose is oxi B-Bridge 1,000 tests 346.50€
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DAMY-100 QuantiChrom™ α-Amylase Assay Kit, Quantitative determination of α-amylase activity by colorimetric (595nm) method Bioassays 100Tests 405.00€ Pub
IRTPAKT Active Rat tPA Functional Assay Innovative Research INC 1 kit(96 Wells) 597.00€ Pub
K4901-100 Adiponectin (human) Elisa Assay Kit Biovision 1 x 96 assays 943.00€ Pub
M0820 Green FAM Caspase 2 Assay Kit 100tests MarkerGene 651.00€
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Pub
1062-200 Caspase-8 Substrate IETD-AFC; Appearance Liquid Biovision 200 assays 311.85€
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Pub
K180-25 CaspGLOW Fluorescein Active Caspase Staining Kit Biovision 25 assays 200.00€
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Pub
  P-4032-48  EpiQuik Total Histone H4 Acetylation Detection Fast Kit (Colorimetric) (48 assays) EpigenTek 358.00€
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Pub
12537 Amplite™ Renilla Luciferase Reporter Gene Assay Kit *Bright Glow* AAT Bioquest 100 plates 3 131.00€
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Pub
22793 Cell Meter™ Phosphatidylserine Apoptosis Assay Kit *Deep Red Fluorescence* AAT Bioquest 1 kit 220.00€
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Pub
936 Magic Red™ Caspases 3 & 7 Assay Kit ImmunoChemistry Technologies I.C.T 100 431.00€ Pub
EOAC-100 EnzyChrom™ Acetate Assay Kit Bioassays 100 tests 277.00€ Pub
K274-100 Total Antioxidant Capacity (TAC) Assay Kit Biovision 100 assays 377.00€ Pub
228-11411-2 Recombinant Rat SCF KITLG Proteins Ray Biotech 10 205.00€ Pub
  OP-0005-2  CytoX Violet Cell Proliferation Cytotoxicity Assay Kit (10x96 assays) EpigenTek 214.00€
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Pub
FA-0010 Transcription Factors: TF Filter Plate Assays Signosis 2011 96 368.00€ Pub
P-1039-96 MethylFlash Urine 5-Methylcytosine (5-mC) Quantification Kit (Colorimetric) (96 assays) EpigenTek 649.00€ Pub
1025T Rat anti-human type I collagen IgG antibody assay kit, TMB Chondrex 425.25€
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Pub
20365T Mouse anti-mouse type II collagen IgG2c antibody subtype assay kit, TMB Chondrex 485.10€
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Pub
4890-25-K MTT Cell Proliferation Assay Kit Trevigen 2500 Tests 274.00€ Pub
ECAM-100 EnzyChrom™ á Amylase Assay Kit Bioassays 100 440.00€ Pub
K122-400 Caspase 5 Fluorometric Assay Kit Biovision 400 assays 738.50€
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K710-100 Xanthine Oxidase Activity Assay Kit Biovision 100 assays 289.00€ Pub
P-2004-96 EpiQuik General Protein DNA Binding Assay Kit Colorimetric EpigenTek 96 assays 521.00€ Pub
1104-1000 Caspase-1 Substrate YVAD-pNA; Appearance Liquid Biovision 1000 assays 614.25€
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Pub
K605-100 PLTP Inhibitor Drug Screening Kit Biovision 100 assays 533.00€ Pub
20324 Mouse anti-bovine type II collagen IgG3 antibody subtype assay kit, OPD Chondrex 400.05€
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Pub
3457-096-K Cultrex 96 Well Collagen I Cell Invasion Assay Trevigen 96 samples 401.00€ Pub
DICA-500 QuantiChrom™ Calcium Assay Kit, Quantitative determination of calcium ion Ca2+ by colorimetric (612nm) method Bioassays 500Tests 320.00€ Pub
K105-400 Caspase 3 Fluorometric Assay Kit Biovision 400 assays 702.50€ Pub
K607-100 Lactate Assay Kit Biovision 100 assays 425.00€ Pub
M0836 Red Sulforhodamine Caspases 3 and 7 Assay Kit 100tests MarkerGene 740.00€
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Pub
1070-1000 Caspase 6 Substrate VEID pNA Biovision 1000 assays 613.31€
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Pub
K190-100 CaspGLOW Red Active Caspase Staining Kit Biovision 100 assays 482.00€
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Pub
13620 Amplite™ Colorimetric Sphingomyelinase Assay Kit *Blue Color* AAT Bioquest 1 kit 202.00€
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Pub
22816 Cell Meter™ Caspase 8 Activity Apoptosis Assay Kit *Red Fluorescence* AAT Bioquest 100 Tests 202.00€
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Pub
ERKD-100 EnzyFluo™ Direct Phospho-ERK Assay Kit Bioassays 100 tests 381.00€ Pub
K335-100 SOD Activity Activity Assay Kit Biovision 100 assays 299.00€ Pub
LF-MA0054 anti-Thioredoxin Reductase 2 (25B3), Mouse monoclonal to Thioredoxin Reductase 2, Isotype IgG1, Host Mouse Abfrontier 100 ul 355.00€ Pub
1003-1000 Annexin V Biotin Reagent1 mg (4000 assays) Biovision 1 mg (4000 assays) 2 073.00€
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Pub
K128-400 Annexin V PE Apoptosis Detection Kit Biovision 400 assays 702.50€
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Pub
  P-4015-48  EpiQuik Global Acetyl Histone H3K18 Quantification Kit (Fluorometric) (48 assays) EpigenTek 370.00€
ASK
Pub
11303 Amplite™ Fluorimetric Monoamine Oxidase Assay Kit *Red Fluorescence* AAT Bioquest 1 kit 163.00€
ASK
Pub
2053 Human monkey anti-porcine type II collagen IgG antibody assay kit, OPD Chondrex 518.81€
ASK
Pub
52190 4x HMT Assay Buffer 4 BPS Bioscience 30 ml 200.00€ Pub
EDLC-100 EnzyChrom™ D-Lactate Assay Kit, Quantitative determination of D-lactic acid by colorimetric (565nm) method. Procedure 20 min. Kit size 100 tests. Detection limit 0.05 mM. Shelf life 6 months. Ship Bioassays 100tests 345.00€ Pub
K127-100 Caspase-4 Colorimetric Assay Kit Biovision 100 assays 406.00€
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K757-100 Glucose-6-Phosphate Dehydrogenase Activity Assay Kit Biovision 100 assays 357.00€ Pub
PBKXL-10 ProPrep™ Genomic XL-10 High-Throughput Genomic DNA Isolation Kits Biotech support group 10, 10ml whole blood 655.00€ Pub
1110-200 Caspase 4 Substrate LEVD pNA Biovision 200 assays 311.85€
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Pub
K810-1000 Protein Quantitation Kit Biovision 1000 assays 240.00€ Pub
11401 Amplite™ Fluorimetric Acetylcholinesterase Assay Kit *Green Fluorescence* AAT Bioquest 1 kit 202.00€
ASK
Pub
2062 Human monkey anti-bovine type II collagen IgA antibody assay kit, OPD Chondrex 518.81€
ASK
Pub
60300 PDE Assay Kit BPS Bioscience 96 reactions 491.00€
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Pub
EFFA-100 EnzyChrom™ Free Fatty Acid Assay Kit, Quantitative determination of free fatty acid by colorimetric (570nm) or fluorimetric (530 590nm) method. Procedure 30 min. Kit size 100 tests. Detection limit Bioassays 100tests 355.00€ Pub
K139-100 Caspase 12 Fluorometric Assay Kit Biovision 100 assays 360.50€
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Pub
POPB-500 PiBlue™ Phosphate Assay Kit Bioassays 500 165.00€ Pub
1113-200 Caspase 10 Substrate AEVD AFC Biovision 200 assays 311.85€
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Pub
OP-0002-1 EpiQuik Nuclear Extraction Kit EpigenTek 100 assays 239.40€ Pub
20351 Mouse anti-human type II collagen IgG1 antibody subtype assay kit, OPD Chondrex 442.05€
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Pub
36336 Cal-520™ Medium Removal Calcium Assay Kit *Optimized for Difficult Cell Lines* AAT Bioquest 10 plates 436.00€
ASK
Pub
DS-PB-02885 Rabbit Anti-Rat 5-alpha Reductase Type 2 Antibodies Ray Biotech 50 502.00€
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Pub
K114-100 Caspase 6 Fluorometric Assay Kit Biovision 100 assays 315.50€
ASK
Pub
K635-100 Creatine Assay Kit Biovision 100 assays 357.00€ Pub
M1376 MarkerGene™ LysoLive™ Lysosomal Phosphatase Assay Kit, Useful for labeling lysosomes in a live cell format and capable of monitoring lysosomal metabolic activity, 1 kit MarkerGene 459.00€
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Pub
1075-200 Caspase 9 Substrate LEHD AFC Biovision 200 assays 311.85€
ASK
Pub
K203-400 Annexin V PE Apoptosis Kit Plus Biovision 400 assays 666.50€
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Pub
20115 Cell Meter™ Live Cell Caspase 8 Binding Assay Kit *Green Fluorescence* AAT Bioquest 25 Tests 280.00€
ASK
Pub
22900 Cell Meter™ Fluorimetric Intracellular Total ROS Activity Assay Kit*Green Fluorescence* AAT Bioquest 1 kit 202.00€
ASK
Pub
CUST-PROD Custom Immunoassay Development Program Assay Development Ray Biotech 0.00€
ASK
Pub
IMTPAKT-TOT Total Mouse tPA Antigen Assay Innovative Research INC 1 kit(96 Wells) 597.00€ Pub
K410-500 Phosphate Colorimetric Assay Kit Biovision 500 assays 201.00€ Pub
LF-PA0055 anti Glutathione Peroxidase 4 Abfrontier 100 ul 320.00€ Pub
1014-1000 Annexin V PE Reagent Biovision 1000 assays 945.50€
ASK
Pub
K159-100 Caspase 9 Inhibitor Drug Screening Kit Biovision 100 assays 387.50€
ASK
Pub
  P-4025-96  EpiQuik Global Acetyl Histone H4K8 Quantification Kit (Fluorometric) (96 assays) EpigenTek 606.00€
ASK
Pub
11954 Amplite™ Fluorimetric Alkaline Phosphatase Assay Kit *Near Infrared Fluorescence* AAT Bioquest 1 kit 202.00€
ASK
Pub
22685 L-xylulose reductase antibody Source Rabbit Polyconal Ab Species Human Application WB SignalWay Antibody S.A.B 100ul 329.00€
ASK
Pub
80203 DPP3 assay kit BPS Bioscience 100 reactions 557.00€
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Pub
EKIN-400 EnzyChrom™ Kinase Assay Kit, Rapid mix-and-measure procedure for determination of kinase activity and high-throµghput screening for kinase inhibitors by fluorimetric (530nm 590nm) method Bioassays 400Tests 250.00€ Pub
K257-100 Cytochrome c Apoptosis Assay Kit Biovision 100 assays 484.00€
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Pub
STA-330 OxiSelect™ TBARS Assay Kit (MDA Quantitation) Cell Biolabs 200 assays 489.00€ Pub
1117-1000 Caspase 12 Substrate ATAD AFC1000 assays Biovision 1000 assays 947.84€
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Pub
P-1034-48 MethylFlash Methylated DNA Quantification Kit Colorimetric EpigenTek 48 assays 458.85€ Pub
1016 Mouse anti-mouse type I collagen IgG antibody assay kit, OPD Chondrex 373.80€
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Pub
20361 Mouse anti mouse type II collagen IgG1 assay kit Chondrex 603.75€ Pub
36401 Cal-520™ PBX Calcium Assay Kit *Optimized for Probenecid-Sensitive Cell Lines* AAT Bioquest 100 plates 3 911.00€
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Pub
EALA-100 EnzyChrom™ L-Alanine Assay Kit Bioassays 100 tests 261.00€ Pub
K118-100 Caspase 9 Fluorometric Assay Kit Biovision 100 assays 342.50€
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Pub
K671-100 Ascorbic Acid Assay Kit II (FRASC) Biovision 100 assays 406.00€ Pub
50CBR101 ImmunoComb Canine Antibody Test Kits: Canine Brucella AJMAL 12tests 171.00€
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Pub
1102-1000 Caspase 5 Substrate WEHD pNA Biovision 1000 assays 634.10€
ASK
Pub
K270-50 FractionPREP™ Cell Fractionation Kit Biovision 50 assays 435.00€ Pub
20315 Mouse anti-chick type II collagen IgG2c antibody subtype assay kit, OPD Chondrex 400.05€
ASK
Pub
30029 NUC VIEW 488 CASPASE 3 ASSAY KIT Biotium 1 KIT 496.65€ Pub
DCRM-250 QuantiChrom™ Chromium Assay Kit Bioassays 250 tests 405.00€ Pub
K006-F1D Glutathione Fluorescent 384-Well Detection Kit Arbor Assays 2x384 well plate 478.00€ Pub
K510-100 Nickel Assay Kit Biovision 100 assays 367.00€ Pub
M0825 Green FAM Caspase 8 Assay Kit 25tests MarkerGene 270.00€
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Pub
1063-1000 Caspase 8 Substrate IETD pNA1000 assays Biovision 1000 assays 514.08€
ASK
Pub
K183-25 Active Caspase 3 Staining Kit, CaspGLOW Fluorescein Biovision 25 assays 165.00€
ASK
Pub
13501 Amplite™ Universal Fluorimetric Protease Activity Assay Kit *Red Fluorescence* AAT Bioquest 1 kit 202.00€ Pub
22798 Cell Meter™ Caspase 8 Activity Apoptosis Assay Kit * Green Fluorescence* AAT Bioquest 1 kit 212.00€
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Pub
A161226 Acetaminophen Glutathione-d3 Disodium Salt C18H19D3N4Na2O8S CAS: TRC 1 mg 277.00€
ASK
Pub
EPRK-100 EnzyChrom™ Pyruvate Kinase Assay Kit Bioassays 100 tests 325.00€ Pub
K302-500 Quick Cell Proliferation Assay Kit Plus Biovision 500 assays 230.00€ Pub
228-11413-1 Recombinant Human SCF KITLG [from Sf9] Proteins Ray Biotech 2 147.00€
ASK
Pub
  P-4009-48  EpiQuik Global Histone H4 Acetylation Assay Kit (48 assays) EpigenTek 474.00€
ASK
Pub
K102-400 Annexin V Cy3 Apoptosis Kit Biovision 400 assays 702.50€ Pub
STA-326 OxiSelect™ Cellular UV-Induced DNA Damage ELISA Kit (CPD) Cell Biolabs 96 assays 489.00€ Pub
1033 Human monkey anti-porcine type I collagen IgG antibody assay kit, OPD Chondrex 528.36€
ASK
Pub
2042T Rat anti-bovine type II collagen IgG antibody assay kit, TMB Chondrex 384.30€
ASK
Pub
50041 Fluorogenic HDAC class 2a Assay kit BPS Bioscience 100 assays 430.00€
ASK
Pub
ECET-100 EnzyChrom™ Ethanol Assay Kit Bioassays 100 338.00€ Pub
K124-100 Caspase 10 Fluorometric Assay Kit Biovision 100 assays 360.50€
ASK
Pub
K723-100 Lipase Assay Kit II Biovision 100 assays 389.00€ Pub
P-3003-2 EpiQuik Histone Methyltransferase Activity Inhibition Assay Kit H3K9 EpigenTek 96 assays 770.00€ Pub
1104-200 Caspase 1 Substrate YVAD pNA200 assays Biovision 200 assays 276.89€
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Pub
K623-100 Cholesterol Cholesteryl Ester Quantitation Kit II Biovision 100 assays 377.00€ Pub
2033 Mouse anti-porcine type II collagen IgG antibody assay kit, OPD Chondrex 384.30€
ASK
Pub
3470-096-K Cultrex In Vitro Angiogenesis Assay Kit (***formerly HT CAS Tube Formation Kit***) Trevigen 96 Tests 298.00€ Pub
DIFE-250 QuantiChrom™ Iron Assay Kit Bioassays 250 269.00€ Pub
K110-100 Caspase 1 Fluorometric Assay Kit Biovision 100 assays 315.50€
ASK
Pub
K616-100 Glucose and Sucrose Assay Kit Biovision 100 assays 386.00€ Pub
M0841 Magic RedTM (LR)2 Cathepsin K Assay Kit 25tests MarkerGene 257.00€
ASK
Pub
1071-1000 Caspase-2 Substrate VDVAD-AFC; Appearance Liquid Biovision 1000 assays 614.25€
ASK
Pub
K193-25 CaspGLOW Red Active Caspase 3 Staining Kit Biovision 25 assays 200.00€
ASK
Pub
13802 Amplite™ Fluorimetric Alanine Aminotransferase Assay Kit AAT Bioquest 1 kit 280.00€
ASK
Pub
22823 Cell Meter™ Fluorometric Caspase 3 7 Activity Assay Kit *Green Fluorescence Optimized for Flow Cytometry* AAT Bioquest 1 kit 231.00€
ASK
Pub
Cat.1005-200 Glutathione Beads Adar Biotech 200 ml 1 616.00€
ASK
Pub
FA-0001 Transcription Factors: NFkB Filter Assay Signosis 2011 96 449.00€ Pub
K357-100 FAD Assay Kit Biovision 100 assays 367.00€
ASK
Pub
LF-MA0090 anti-Glutathione Peroxidase 1 (2A10) , Mouse monoclonal to Glutathione Peroxidase 1, Isotype IgG1, Host Mouse Abfrontier 100 ul 355.00€ Pub
1007-1000 Caspase-3 Substrate DEVD-AFC; Appearance Liquid Biovision 1000 assays 614.25€
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Pub
K131-7-25 Caspase Fluorometric Substrate Set Biovision 7 x 25 assays 559.00€ Pub
  P-4017-96  EpiQuik Global Acetyl Histone H3K23 Quantification Kit (Fluorometric) (96 assays) EpigenTek 618.00€
ASK
Pub
15259 Amplite™ Fluorimetric NADP NADPH Assay Kit *Red Fluorescence* AAT Bioquest 1 kit 280.00€
ASK
Pub
22828 Cell Meter™ Annexin V Binding Apoptosis Assay Kit *Blue Fluorescence Excited at 405 nm* AAT Bioquest 1 kit 202.00€
ASK
Pub
CM015 CHO-CM ELISA Kit ;Host cell protein assay, 96 tests Cyg Tech 905.00€ Pub
IHPAIUPAKT-COM Complex Human PAI-1-uPA Antigen Assay Innovative Research INC 1 kit(96 Wells) 597.00€ Pub
K372-100 cGMP Activity Assay Kit Biovision 100 assays 367.00€
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Pub
LF-P0009 Thioredoxin Reductase, Yeast, Protein Abfrontier 0.5 mg 218.00€ Pub
1007-200 Caspase 3 Substrate DEVD AFC200 assays Biovision 200 assays 276.00€
ASK
Pub
K136-7-25 Caspase Colorimetric Substrate Set Plus Biovision 7 x 25 assays 726.00€ Pub
  P-4020-48  EpiQuik Global Acetyl Histone H3K56 Quantification Kit (Colorimetric) (48 assays) EpigenTek 370.00€
ASK
Pub
11503 Amplite™ Intracellular Fluorimetric Hydrogen Peroxide Assay Kit *Green Fluorescence* AAT Bioquest 1 kit 202.00€
ASK
Pub
21610 PhosphoWorks™ Luminometric ATP Assay Kit *Bright Glow* AAT Bioquest 1 kit 124.00€
ASK
Pub
60400 PDE10A Assay Kit BPS Bioscience 96 reactions 491.00€
ASK
Pub
EFRU-100 EnzyChrom™ Fructose Assay Kit, Quantitative determination of fructose by colorimetric (565nm) method. Procedure 70 min. Kit size 100 tests. Detection limit 12 µM fructose. Shelf life 3 months. Shi Bioassays 100tests 335.00€ Pub
K143-100 Cathepsin D Activity Assay Kit Biovision 100 assays 357.00€
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Pub
QFAP-100 QuantiFluo™ Alkaline Phosphatase Assay Kit Bioassays 100 tests 141.00€ Pub
1114-200 Caspase-10 Substrate AEVD-pNA; Appearance Liquid Biovision 200 assays 311.85€
ASK
Pub
P-1014-48  Methylamp Global DNA Methylation Quantification Kit (48 assays) EpigenTek 446.25€
ASK
Pub
10054 Amplite™ Fluorimetric Glutamic Acid Assay Kit *Red Fluorescence* AAT Bioquest 1 kit 163.00€
ASK
Pub
20352T Mouse anti-human type II collagen IgG2a antibody subtype assay kit, TMB Chondrex 442.05€
ASK
Pub
36341 Screen Quest™ Fluorimetric MDR Assay Kit *10 Plates* AAT Bioquest 1 kit 791.00€
ASK
Pub
DURE-100 QuantiChrom™ Urease Assay Kit Bioassays 100 tests 285.00€ Pub
K115-200 Caspase 6 Colorimetric Assay Kit Biovision 200 assays 450.50€
ASK
Pub
K646-100 Glycogen Assay Kit 100 assays Biovision 100 assays 364.51€ Pub
M1575 RubyGlowTM Luminescent Cell Viability Assay Kit, Contains a luciferase emitting red light, generates very stable luminescent signals. Allows multiplexing with currently available luciferase in cell-ba MarkerGene 187.00€
ASK
Pub
1076-200 Caspase-9 Substrate LEHD-pNA; Appearance Liquid Biovision 200 assays 311.85€
ASK
Pub
K256-25 Mitochondria/Cytosol Fractionation Kit Biovision 25 assays 187.11€
ASK
Pub
20311 Mouse anti-chick type II collagen IgG1 antibody subtype assay kit, OPD Chondrex 400.05€
ASK
Pub
290-63701 Triglyceride Assay Kit Lipids in serum consist of triglycerides, cholesterols, phospholipids, free fatty acids and slight amounts of fat soluble components such as fatsoluble vitamins and carotenes. T B-Bridge 1,000 tests 467.25€
ASK
Pub
DADH-100 QuantiChrom™ Alcohol dehydrogenase Assay Kit Bioassays 100 tests 325.00€ Pub
IPTPAKT Active porcine tPA functional assay ELISA kit Innovative Research INC 1 kit(96 Wells) 597.00€ Pub
K420-100 Phosphate Fluorometric Assay Kit Biovision 100 assays 260.00€ Pub
M0795 MarkerGeneTM Live Dead Assay Kit, Allows fast and easy measurement of both living and dead cells by measuring intracellular esterase activity(live cells) and staining of nucleic acid in chromatin (dea MarkerGene 299.00€
ASK
Pub
1015-1000 Annexin V PE Cy5 Reagent1000 assays Biovision 1000 assays 984.00€
ASK
Pub
K172-100 CaspGLOW Fluorescein Active Caspase 12 Staining Kit Biovision 100 assays 521.00€
ASK
Pub
  P-4028-48  EpiQuik Global Acetyl Histone H4K12 Quantification Kit (Colorimetric) (48 assays) EpigenTek 358.00€
ASK
Pub
12519 Amplite™ Luciferase Reporter Gene Assay Kit *Bright Glow* AAT Bioquest 10 plates 358.00€
ASK
Pub
22786 Cell Meter™ Cell Viability Assay Kit *Green Fluorescence* AAT Bioquest 1 kit 202.00€
ASK
Pub
80210 FAP assay kit BPS Bioscience 100 reactions 856.00€
ASK
Pub
ENEU-100 EnzyChrom™ Neuraminidase Assay Kit Bioassays 100 tests 357.00€ Pub
  OP-0001-2  EpiQuik Superoxide Dismutase Activity Inhibition Assay Kit (Colorimetric) (2x96 assays) EpigenTek 240.00€
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Pub
291-59201 GLP 1 ELISA Kit, Rat Glucagon like peptide 1 (GLP 1) is an incretin hormone, which is synthesized in intestinal endocrine cells. This peptide is known to increase insulin secretion by glucose stimulat B-Bridge 96 assays 1 040.55€
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Pub
P-1036-48  MethylFlash Hydroxymethylated DNA Quantification Kit (Colorimetric) (48 assays) EpigenTek 514.94€
ASK
Pub
1022T Rat anti-bovine type I collagen IgG antibody assay kit, TMB Chondrex 373.80€
ASK
Pub
20363 Mouse anti-mouse type II collagen IgG2b antibody subtype assay kit, OPD Chondrex 485.10€
ASK
Pub
4677-096-K Universal Colorimetric PARP Assay Kit w Histone Coated Strip Wells Trevigen 96 Samples 484.00€ Pub
EASC-100 EnzyChrom™ Ascorbic Acid Assay Kit, Quantitative determination of ascorbic acid by colorimetric (570nm) or fluorimetric (530nm 590nm) methods. Procedure 10 min. Kit size 100 tests. Detection limit Bioassays 100tests 335.00€ Pub
K119-200 Caspase 9 Colorimetric Assay Kit Biovision 200 assays 468.50€
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K689-100 PicoProbe™ Fructose-6-Phosphate Assay Kit Biovision 100 assays 425.00€ Pub
K-3034-2 Kits for 96 well Plate Vacuum Manifold AccuPrep™ PCR Purification kit Bioneer 96-well plate X 2 305.00€
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1103-1000 Caspase-1 Substrate YVAD-AFC; Appearance Liquid Biovision 1000 assays 614.25€
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K347-100 NADP NADPH Quantitation Kit Biovision 100 assays 435.00€ Pub
20322 Mouse anti-bovine type II collagen IgG2a antibody subtype assay kit, OPD Chondrex 400.05€
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31001 Amplite™ Universal Fluorimetric Kinase Assay Kit *Red Fluorescence* AAT Bioquest 1 kit 280.00€
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DIAG-250 QuantiChrom™ BCG Albumin Assay Kit Bioassays 250 299.00€ Pub
K008-F1 Glutathione S Transferase Fluorescent Kit Arbor Assays 1x96 well plate 293.00€
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K566-100 Sialic Acid (NANA) Assay Kit Biovision 100 assays 435.00€ Pub
M0830 Green FAM Caspase 10 Assay Kit 100tests MarkerGene 651.00€
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1069-1000 Caspase 6 Substrate VEID AFC Biovision 1000 assays 613.31€
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K188-25 CaspGLOW Fluorescein Active Caspase 8 Staining Kit Biovision 25 assays 200.00€
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13511 Amplite™ Universal Fluorimetric MMP Activity Assay Kit *Red Fluorescence* AAT Bioquest 1 kit 280.00€
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22803 Cell Meter™ NIR Mitochondria Membrane Potential Assay Kit *Optimized for Microplate Reader* AAT Bioquest 1 kit 231.00€
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ER-14-0416 Goat Anti-Human Dicarbonyl Reductase, (C Terminus) Antibodies Ray Biotech 100 μg 353.00€
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K313-500 LDH Cytotoxicity Assay Kit II Biovision 500 assays 270.50€
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LF-MA0015 anti-Thioredoxin Reductase 1 (19A1), Mouse monoclonal to Thioredoxin Reductase 1, Isotype IgG1, Host Mouse Abfrontier 100 ul 355.00€ Pub
1001-200 Annexin V FITC Reagent200 assays Biovision 200 assays 325.00€
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K109-100 Annexin V Biotin Apoptosis Kit Biovision 100 assays 333.50€
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  P-4012-48  EpiQuik Global Acetyl Histone H3K14 Quantification Kit (Colorimetric) (48 assays) EpigenTek 370.00€
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1042 Human monkey anti-bovine type I collagen IgA antibody assay kit, OPD Chondrex 528.36€
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2045 Rat anti-human type II collagen IgG antibody assay kit, OPD Chondrex 434.70€
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52004 Methyltransferase PRMT1 Assay Kit BPS Bioscience 100 reactions 530.00€
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ECOA-100 EnzyChrom™ Coenzyme A Assay Kit Bioassays 100 tests 277.00€ Pub
K125-200 Caspase 10 Colorimetric Assay Kit Biovision 200 assays 495.50€ Pub
K744-100 Myeloperoxidase (MPO) Colorimetric Activity Assay Kit Biovision 100 assays 406.00€ Pub
P-3016-096 EpiQuik In Situ Histone H3K9 Methylation Assay Kit EpigenTek 96 assays 584.00€ Pub
1109-200 Caspase 4 Substrate LEVD AFC Biovision 200 assays 311.85€
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K790-1000 StayBrite™ Highly Stable Luciferase Luciferin Reagent Biovision 1000 assays 728.00€ Pub
20332 Mouse anti porcine type II collagen IgG2a assay kit Chondrex 497.70€
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36005 Screen Quest™ Membrane Potential Assay Kit *Red Fluorescence* AAT Bioquest 10 plates 436.00€
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DIHM-250 QuantiChrom™ Heme Assay Kit Bioassays 250 340.00€ Pub
K111-200 Caspase 1 Colorimetric Assay Kit Biovision 200 assays 450.50€
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K621-100 Galactose Assay Kit Biovision 100 assays 357.00€ Pub
M11026740 Glutathione reduced CAS Number [70 18 8] Molekula 25 G 166.00€
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1071-200 Caspase 2 Substrate VDVAD AFC200 assays Biovision 200 assays 276.89€
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K199-25 CaspGLOW Red Active Caspase 9 Staining Kit Biovision 25 assays 200.00€
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20334 Mouse anti-porcine type II collagen IgG3 antibody subtype assay kit, OPD Chondrex 400.05€
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36308 Screen Quest™ Fluo 8 Medium Removal Calcium Assay Kit *Optimized for Difficult Cell Lines* AAT Bioquest 10 Plates 241.00€
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DIUR-500 QuantiChrom™ Urea Assay Kit ACR 500 249.00€ Pub
K112-25 Caspase 8 Fluorometric Assay Kit Biovision 25 assays 162.50€
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K628-100 Maltose Assay Kit Biovision 100 assays 357.00€ Pub
M1245 MarkerGeneTM Fluorescent Cellulase Assay Kit, Allows fast and easy detection of most cellulases in a microtiter plate based assay format, 1 kit MarkerGene 367.82€
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1072-200 Caspase 2 Substrate VDVAD pNA Biovision 200 assays 311.85€
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K201-400 Annexin V FITC Apoptosis Kit Plus Biovision 400 assays 666.50€
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15264 Amplite™ Fluorimetric NADP NADPH Ratio Assay Kit *Red Fluorescence* AAT Bioquest 1 kit 319.00€
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22835 Cell Meter™ Phosphatidylserine Apoptosis Assay Kit *Blue Fluorescence Excited at 405 nm* AAT Bioquest 1 kit 202.00€
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CQLD-100 QuantiChrom™ LDH Cytotoxicity Assay Kit, Quantitative determination of cytotoxicity based on cellular lactate dehydrogenase release into cell culture medium by colorimetric (565nm) method. Procedure Bioassays 100tests 113.00€ Pub
IHUPAKT-TOT Total Human uPA Antigen Assay Innovative Research INC 1 kit(96 Wells) 597.00€ Pub
K387-100 Zinc Assay Kit Biovision 100 assays 357.00€ Pub
1008-200 Caspase 3 Substrate DEVD pNA Biovision 200 assays 311.85€
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K153-100 Caspase 3 Inhibitor Drug Screening Kit Biovision 100 assays 387.50€
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  P-4022-96  EpiQuik Global Acetyl Histone H4K5 Quantification Kit (Colorimetric) (96 assays) EpigenTek 645.00€
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11553 Amplite™ Fluorimetric Peroxidase Assay Kit *Near Infrared Fluorescence* AAT Bioquest 1 kit 280.00€
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21665 PhosphoWorks™ Colorimetric Phosphate Assay Kit *Blue Color* AAT Bioquest 1 kit 202.00€
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7501-500-K HT Superoxide Dismutase Assay Kit Trevigen 480 tests 547.00€ Pub
EGPX-100 EnzyChrom™ Glutahione Peroxidase Assay Kit Bioassays 200 274.00€ Pub
K786-100 Glucose Dehydrogenase Activity Assay Kit Biovision 100 assays 367.00€ Pub
SKT-106 Hsc70 ELISA kit Sample Ttpecell lysates, tissue extracts, serum samples Assay Range 150-2.34ng mL Stressmarq 96 well 629.00€ Pub
1116-1000 Caspase 13 Substrate LEED AFC1000 assays Biovision 1000 assays 541.49€
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P-1014B-96 Methylamp Global DNA Methylation Quantification Ultra Kit EpigenTek 96 assays 808.50€
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1011T Mouse anti-chick type I collagen IgG antibody assay kit, TMB Chondrex 373.80€
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20354T Mouse anti-human type II collagen IgG3 antibody subtype assay kit, TMB Chondrex 442.05€
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