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Search results for: Isotype

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#35760121   2022/06/24 To Up

Effect of the ADCC-modulating mutations and the selection of human IgG isotypes on physicochemical properties of Fc.

Monoclonal antibodies, particularly IgGs and Ig-based molecules, are a well-established and growing class of biotherapeutic drugs. In order to improve efficacy, potency and pharmacokinetics of these therapeutic drugs, pharmaceutical industries have investigated significantly in engineering fragment crystallizable (Fc) domain of these drugs to optimize the interactions of these drugs and Fc gamma receptors (FcγRs) in recent ten years. The biological function of the therapeutics with the antibody-dependent cellular cytotoxicity (ADCC) enhanced double mutation (S239D/I332E) of isotype IgG1, the ADCC reduced double mutation (L234A/L235A) of isotype IgG1, and ADCC reduced isotype IgG4 has been well understood. However, limited information regarding the effect of these mutations or isotype difference on physicochemical properties (PCP), developability, and manufacturability of therapeutics bearing these different Fc regions is available. In this report, we systematically characterize the effects of the mutations and IgG4 isotype on conformation stability, colloidal stability, solubility, and storage stability at accelerated conditions in two buffer systems using six Fc variants. Our results provide a basis for selecting appropriate Fc region during development of IgG or Ig-based therapeutics and predicting effect of the mutations on CMC development process.
Helen Haixia Wu, Maureen Crames, Yangjie Wei, Dongmei Liu, Kristina Gueneva-Boucheva, Ikbae Son, Lee Frego, Fei Han, Rachel Kroe-Barrett, Andrew Nixon, Marlow Michael

1098 related Products with: Effect of the ADCC-modulating mutations and the selection of human IgG isotypes on physicochemical properties of Fc.

102 5 G 100ul100 TESTS1mg2 1 ml100 ul200 units0.2 mg

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#35758592   // To Up

THE USE OF INTRADERMAL SKIN TESTING AND HYPOSENSITIZATION INJECTIONS TO CONTROL SEASONAL DERMATITIS IN GREATER ONE-HORNED RHINOCEROSES ().

Allergic dermatitis was diagnosed in a 25-yr-old female greater one-horned rhinoceros () and her 6-yr-old female offspring by skin biopsy, intradermal skin testing (IDST), and allergen-specific serum IgE testing. Dam and offspring presented with seasonal, erosive, and ulcerative dermatitis affecting the face, legs, and trunk starting at 6 and 2 yr of age, respectively. IDST was performed at the caudal pinnal base using 61 regionally specific allergens. Specific serum allergen responses were detected using Heska's Equine ALLERCEPT Allergen Panel. Histopathology of the lesions was consistent with an allergic etiology. Injectable allergen-specific immunotherapy was initiated in both animals and within 6 to 18 mon after commencing hyposensitization clinical improvement was noted. This report documents a repeatable methodology for IDST and serological allergen testing for use in rhinoceroses. The hyposensitization protocol detailed here can help guide future treatment protocols.
Sarah B Chaney, Melissa Loewinger, Donna Doherty, Colleen M McCann, Kenneth J Conley, Denise McAloose, Andrew Rosenberg, John M Sykes

1984 related Products with: THE USE OF INTRADERMAL SKIN TESTING AND HYPOSENSITIZATION INJECTIONS TO CONTROL SEASONAL DERMATITIS IN GREATER ONE-HORNED RHINOCEROSES ().



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#35758480   // To Up

SINGLE NUCLEOTIDE POLYMORPHISM RS4696480 OF TLR2 GENE ASSOCIATES WITH SEVERITY OF ATOPIC DERMATITIS IN CHILDREN, BUT NOT WITH IGE SENSITIZATION TO MALASSEZIA.

The aim: Malassezia has been linked to atopic dermatitis, and TLRs are suggested to mediate influence of Malassezia spp on human cells. The aim of the study was to examine if TLR2 rs4696480 polymorphism predisposes to atopic dermatitis, IgE sensitization to Malassezia or to severe phenotype of atopic dermatitis.
Olena V Mozyrska, Oleksandr P Volosovets, Sergii P Kryvopustov, Sergii V Goncharov, Anna V Kupkina, Oksana V Iemets, Victor E Dosenko

2138 related Products with: SINGLE NUCLEOTIDE POLYMORPHISM RS4696480 OF TLR2 GENE ASSOCIATES WITH SEVERITY OF ATOPIC DERMATITIS IN CHILDREN, BUT NOT WITH IGE SENSITIZATION TO MALASSEZIA.

1ml1ml5ug1096 wells (1 kit)1 mg1 kit

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#35758452   // To Up

ANTIBIOTIC ASSOCIATIVE DISORDERS OF THE MICROBIOCENOSIS OF THE COLON IN INFANTS WITH ACUTE RESPIRATORY DISEASES.

The aim: Study the effect of antibiotics of different groups on the condition of the colon microflora in infants with acute respiratory pathology.
Veronika M Dudnyk, Iryna I Andrikevych, Katerina Khromykh, Halyna I Mantak, Henadii M Rudenko

1568 related Products with: ANTIBIOTIC ASSOCIATIVE DISORDERS OF THE MICROBIOCENOSIS OF THE COLON IN INFANTS WITH ACUTE RESPIRATORY DISEASES.

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#35758390   2022/06/24 To Up

Contribution of interleukin 27 serum level to pathogenesis and prognosis in children with immune thrombocytopenia.

Concepts surrounding the mechanisms of thrombocytopenia in ITP have shifted from the traditional view of autoantibody mediated platelet destruction to more complex mechanisms in which impaired platelet production, T-cell-mediated effects, and disturbed cytokine profiles play a role. Interleukin 27 (IL-27) plays pleiotropic roles in immunomodulation and autoimmune diseases.We aimed to determine the level of IL-27 in patients with ITP and its relationship to patient and disease characteristics as well as disease chronicity and response to treatment.Sixty childrens with primary immune thrombocytopenia were consequetively enrolled in this study as well as 20 age and sex matched healthy controls.ITP patients had significantly higher levels of IL-27 than controls (770.6 and 373.8 pg/ml, respectively). Patients with acute ITP had the highest levels of IL-27 among patient groups, while patients in remission had the lowest IL-27 levels (860.1and 622.9 pg/ml, respectively). Patients who received IVIG and combined steroids plus IVIG had significantly higher IL-27 levels than others. Patients who received Eltrombopag had significantly lower IL-27 levels than others.IL-27 seems to play a role in pathogenesis of childhood ITP. IL-27 can be used as a predictor for disease occurrence as well as responsiveness to treatment.
Tamer Hassan, Doaa Abdel Rahman, Nermin Raafat, Manar Fathy, Mohamed Shehab, Ahmed Hosny, Reham Fawzy, Marwa Zakaria

2026 related Products with: Contribution of interleukin 27 serum level to pathogenesis and prognosis in children with immune thrombocytopenia.

96 wells (1 kit)96T2ug2 100.00 ug10 96T96 wells (1 kit)

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#35757764   2022/06/09 To Up

Immunogenicity, Effectiveness, and Safety of Inactivated Virus (CoronaVac) Vaccine in a Two-Dose Primary Protocol and BNT162b2 Heterologous Booster in Brazil (Immunita-001): A One Year Period Follow Up Phase 4 Study.

Effective and safe vaccines against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are critical to controlling the COVID-19 pandemic and will remain the most important tool in limiting the spread of the virus long after the pandemic is over.
Rafaella F Q Grenfell, Nathalie B F Almeida, Priscilla S Filgueiras, Camila A Corsini, Sarah V C Gomes, Daniel A P de Miranda, Adelina J Lourenço, Olindo A Martins-Filho, Jaquelline G de Oliveira, Andrea Teixeira-Carvalho, Guilherme R F Campos, Mauricio L Nogueira, Pedro Augusto Alves, Gabriel R Fernandes, Leda R Castilho, Tulio M Lima, Daniel P B de Abreu, Renata G F Alvim, Thaís Bárbara de S Silva, Wander de J Jeremias, Dayane A Otta, Ana Carolina Campi-Azevedo,

1121 related Products with: Immunogenicity, Effectiveness, and Safety of Inactivated Virus (CoronaVac) Vaccine in a Two-Dose Primary Protocol and BNT162b2 Heterologous Booster in Brazil (Immunita-001): A One Year Period Follow Up Phase 4 Study.

100 50ug100100 10mg100 1 mg14/120 Packing /sleeve/bo1 mg100 50ug

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#35757744   2022/06/10 To Up

Efficacy of Whole-Blood Exchange Transfusion in Refractory Severe Autoimmune Haemolytic Anaemia Secondary to Systemic Lupus Erythematosus: A Real-World Observational Retrospective Study.

Severe autoimmune haemolytic anaemia (AIHA) in systemic lupus erythematosus (SLE) patients could be life-threatening and formidable, especially in those nonresponsive to glucocorticoids (GCs) and immunosuppressants (ISAs). Whole-blood exchange transfusion (WBE), with plasma exchange and pathogenic cell removal as well as healthy red blood cell transfusion, could be beneficial. The objective of this study was to investigate the efficacy and safety of WBE in combination with GCs/ISAs.
Ying Jiang, Hong Jun Zhao, Hui Luo, Bi Juan Li, Zhi Min Zhang, Li Dan Zhao, Xiao Xia Zuo

2272 related Products with: Efficacy of Whole-Blood Exchange Transfusion in Refractory Severe Autoimmune Haemolytic Anaemia Secondary to Systemic Lupus Erythematosus: A Real-World Observational Retrospective Study.

100 251 kit251 kit100 1 mg 100 UG

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#35757743   2022/06/10 To Up

The Cross-Protective Immunity Landscape Among Different SARS-CoV-2 Variant RBDs.

Despite the fact that SARS-CoV-2 vaccines have been available in most parts of the world, the epidemic status remains grim with new variants emerging and escaping the immune protection of existing vaccines. Therefore, the development of more effective antigens and evaluation of their cross-protective immunity against different SARS-CoV-2 variants are particularly urgent. In this study, we expressed the wild type (WT), Alpha, Beta, Delta, and Lambda RBD proteins to immunize mice and evaluated their cross-neutralizing activity against different pseudoviruses (WT, Alpha, Beta, Delta, Lambda, and Omicron). All monovalent and pentavalent RBD antigens induced high titers of IgG antibodies against different variant RBD antigens. In contrast, WT RBD antigen-induced antibodies showed a lower neutralizing activity against Beta, Delta, Lambda, and Omicron pseudoviruses compared to neutralization against itself. Interestingly, Beta RBD antigen and multivalent antigen induced broader cross-neutralization antibodies than other variant RBD antigens. These data provide a reference for vaccine strain selection and universal COVID-19 vaccine design to fight the constant emergence of new SARS-CoV-2 variants.
Wenqiang Sun, Lihong He, Huicong Lou, Wenhui Fan, Limin Yang, Gong Cheng, Wenjun Liu, Lei Sun

1796 related Products with: The Cross-Protective Immunity Landscape Among Different SARS-CoV-2 Variant RBDs.

2000 IU15ug10 mg 5 G250ul 1 G250ul1 g250ul250ul

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#35757724   2022/06/02 To Up

Serum Immunoglobulins, Pneumonia Risk, and Lung Function in Middle-Aged and Older Individuals: A Population-Based Cohort Study.

Immunoglobulins (Igs) play a pivotal role in host defense and prevention of pneumonia. Aging influences serum Ig levels, but the association between Igs and pneumonia in community-dwelling older individuals remains unknown. We evaluated the association of serum IgA, IgG, and IgM with pneumonia and lung function in middle-aged and older individuals.
Samer R Khan, Anna Vanoverschelde, Lies Lahousse, Robin P Peeters, P Martin van Hagen, Guy Brusselle, Layal Chaker, Virgil A S H Dalm

1247 related Products with: Serum Immunoglobulins, Pneumonia Risk, and Lung Function in Middle-Aged and Older Individuals: A Population-Based Cohort Study.

96T200ug25 mg2.5 mg1000 tests

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#35757719   2022/06/01 To Up

Clinical Significance of Serum Albumin and Implications of FcRn Inhibitor Treatment in IgG-Mediated Autoimmune Disorders.

Serum albumin (SA), the most abundant soluble protein in the body, maintains plasma oncotic pressure and regulates the distribution of vascular fluid and has a range of other important functions. The goals of this review are to expand clinical knowledge regarding the functions of SA, elucidate effects of dysregulated SA concentration, and discuss the clinical relevance of hypoalbuminemia resulting from various diseases. We discuss potential repercussions of SA dysregulation on cholesterol levels, liver function, and other processes that rely on its homeostasis, as decreased SA concentration has been shown to be associated with increased risk for cardiovascular disease, hyperlipidemia, and mortality. We describe the anti-inflammatory and antioxidant properties of SA, as well as its ability to bind and transport a plethora of endogenous and exogenous molecules. SA is the primary serum protein involved in binding and transport of drugs and as such has the potential to affect, or be affected by, certain medications. Of current relevance are antibody-based inhibitors of the neonatal Fc receptor (FcRn), several of which are under clinical development to treat immunoglobulin G (IgG)-mediated autoimmune disorders; some have been shown to decrease SA concentration. FcRn acts as a homeostatic regulator of SA by rescuing it, as well as IgG, from intracellular degradation a common cellular recycling mechanism. Greater clinical understanding of the multifunctional nature of SA and the potential clinical impact of decreased SA are needed; in particular, the potential for certain treatments to reduce SA concentration, which may affect efficacy and toxicity of medications and disease progression.
E Sally Ward, Deborah Gelinas, Erwin Dreesen, Jolien Van Santbergen, Jan Terje Andersen, Nicholas J Silvestri, Joseph E Kiss, Darrell Sleep, Daniel J Rader, John J P Kastelein, Els Louagie, Gestur Vidarsson, Isabel Spriet

1308 related Products with: Clinical Significance of Serum Albumin and Implications of FcRn Inhibitor Treatment in IgG-Mediated Autoimmune Disorders.

100 assays5 mg10mg 5 G100 ml20 ul (10 mM)1 mg200ul

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