Gentaur Pub

#36481482   2022/11/30 To Up

Effects of oil-based adjuvants on the immune response of pigs after dermal administration of antigen and evaluation of the immunization level after a subsequent Actinobacillus pleuropneumoniae challenge in pigs.

Route of vaccine delivery can greatly impact the immunogenicity, efficacy and safety of the vaccine. Four groups of piglets were immunised transdermally (t.d.), intradermally (i.d.) or intramuscularly (i.m.) with the same doses of antigen in combination with a water-in-oil-in-water emulsion adjuvant Montanide™ ISA 201 VG or with a microemulsion adjuvant Montanide™ IMS 1313 VG N ST (Seppic, France). The last group was left without vaccination as a control group. All animals were subsequently exposed to the infection induced by Actinobacillus pleuropneumoniae (App). The immune response was evaluated with respect to the intensity of systemic and mucosal antibody formation, their isotype characterisation and rate of cell-mediated immunity. These findings were compared with the intensity of adverse local reactions and level of protection in experimental challenge. Monitoring of the local reaction at the injection site after each administration showed that microemulsion adjuvant IMS 1313 was less reactogenic than the water-in-oil-in-water emulsion ISA 201. In terms of efficacy, both dermal administrations were less immunogenic than the i.m route. The i.m. injection induced higher anti-App9 IgG and IgM titres. Nevertheless, IgG1 and IgG2 isotypes analysis revealed a close immunological profile between i.m. and i.d. routes. The concentration of IFN-γ from peripheral blood after in vitro restimulation with the specific antigen was only increased in the i.m. group at the day of challenge (D35) and two weeks after (D49). Interestingly, the smallest gross pulmonary lesions were observed in the i.d. vaccinated group (3.4%) compared to the control group (39.4%) and to groups with other routes of administration. Taken together, these results suggest that i.d. administration of vaccines is a promising approach. Even the i.d. vaccine was more reactogenic and slightly less immunogenic than the i.m. vaccine, its protection effectiveness seemed to be superior.
Rea Jarosova, Juliette Ben Arous, Katerina Nechvatalova, Katerina Nedbalcova, Karolina Hlavova, Hana Stepanova, Lenka Leva, Zrinka Oreskovic, Jan Matiasovic, Nicolas Versillé, Zbysek Sladek, Martin Faldyna

2697 related Products with: Effects of oil-based adjuvants on the immune response of pigs after dermal administration of antigen and evaluation of the immunization level after a subsequent Actinobacillus pleuropneumoniae challenge in pigs.

5 G150ul (1mg/ml)1 mg1-99 mg/ml/ea price x 2

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#36481358   2022/12/05 To Up

Activation of B cells in Tertiary Lymphoid Structures in cancer: Anti-tumor or anti-self?

Whereas T cells in the tumor microenvironment have been the main focus as cancer controlling cells and targets of immunotherapies, B cells have recently gained strong attention. Being associated to Tertiary Lymphoid Structures (TLS) located at the vicinity of tumor nests, the fate of B cell depends on TLS maturity. In immature TLS they may evolve as regulatory B cells producing immunosuppressive cytokines and promote tumor growth. In mature TLS with a germinal center, B cells are selected, amplified, undergo affinity maturation and isotypic switching, resulting in plasma cell generation and production of anti-tumor antibodies. In that case, they are associated with longer patient's survival and therapeutic response to immunotherapy. Identification of tumor specific, or tumor overexpressed, antigens recognized by "in situ" produced antibodies and their discrimination from self-antigens induced by ICI treatments is a major challenge to develop novel antibody-based immunotherapies.
Wolf H Fridman, Sophie Sibéril, Guilhem Pupier, Sarah Soussan, Catherine Sautès-Fridman

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#36474577   // To Up

PPD in HBsAg vaccine formulation suppressed IFN-γ and IL-4 cytokine responses and induced long-lived humoral immune responses: Results from 220-day monitoring of specific IgG responses.

Here, immune responses and long-lived IgG responses of HBsAg-Alum, HBsAg-MF59, as well as HBsAg-MF59 were compared when formulated with PPD.
Rayhaneh Mirzaee, Fahimeh Nemati, Mina Mirzaee, Bahareh Golkaran, Akbar Khorasani, Niloufar Rashedi, Fatemeh Asgarhalvaei, Mohammad Ali Savoji, Mehdi Mahdavi

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#36473374   2022/11/04 To Up

Research Note: Genome-wide association study for natural antibodies and resilience in a purebred layer chicken line.

Resilience is the capacity of an animal to be minimally affected by disturbances or rapidly return to the state pertained before exposure to a disturbance. Resilience indicators can be estimated from longitudinal production data, using deviations of observed from expected production levels. One component of resilience is disease resilience, which includes general disease resistance. Natural antibodies (NAbs) are an indicator trait for general disease resistance. The aim of this study was to perform a genome-wide association study (GWAS) for resilience indicators and NAbs in a Rhode Island purebred layer line and study potential overlap in genomic regions detected for these traits. For 2,494 hens, deviations (i.e., differences) between observed weekly egg production and expected weekly egg production were calculated. Resilience indicators were then defined as the natural logarithm of the variance of deviations, skewness of deviations, and lag-one autocorrelation of deviations. For a subset of 1,221 hens genotyped with the 60 K Illumina SNP BeadChip, NAbs binding keyhole-limpet hemocyanin were available (isotypes IgM and IgG). Heritabilities, estimated with a linear mixed animal model, were 0.39 for IgM and 0.20 for IgG, and ranged from 0.03 to 0.18 for the resilience indicators. No significant associations were found in the GWAS, except for a single chromosomal region for the skewness of egg deviations in wk 25 to 83 of the laying period. The absence of significant peaks for NAbs and resilience indicators suggests that there are no genes with major effect and that the traits are likely under polygenic control in this line.
Harmen P Doekes, Henk Bovenhuis, Tom V L Berghof, Katrijn Peeters, Jeroen Visscher, Han A Mulder

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#36472272   // To Up

THE ROLE OF ENTEROVIRUSES IN THE DEVELOPMENT OF ISCHEMIC STROKE AND ITS OUTCOMES.

The aim: To study the role of enteroviruses (EV) in the development of ischemic stroke and its outcome.
Nataliia S Turchyna, Tatiana M Cherenko, Natalia G Andriushkova, Valentyna V Melnyk, Olena V Kuzminska, Yuliya L Heletiuk

2051 related Products with: THE ROLE OF ENTEROVIRUSES IN THE DEVELOPMENT OF ISCHEMIC STROKE AND ITS OUTCOMES.

1100 U100.00 ul1 ml

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#36471962   // To Up

[Jingfang Mixture regulates balance of spleen T lymphocyte subsets in urticaria mice by inhibiting JAK2-STAT3 signaling pathway].

Urticaria is an immune-mediated allergic disease. This study explored the effect of Jingfang Mixture on spleen T lymphocyte subsets of urticaria mice. A total of 50 Kunming mice were randomized into normal group(C), model group(V), and low-(JF-L, 0.5 g·kg~(-1)), medium-(JF-M, 1 g·kg~(-1)) and high-dose(JF-H, 2 g·kg~(-1)) Jingfang Mixture groups, with 10 mice in each group. The mixture of ovalbumin and aluminum hydroxide(0.1 mg + 0.1 mL) was used(intraperitoneal injection) to induce urticaria in mice. The administration began 6 days after the first immunization, and the second immunization was carried out 10 days after the first immunization. The pruritus index was detected within 30 min after the second immunization. The administration lasted 21 days. After 21 days, the serum was taken to detect the total IgE level. Based on hematoxylin and eosin(HE) staining, the pathological changes of skin tissue were observed, and Western blot was used to detect the levels of p-Janus kinase 2(JAK2)/JAK2 and p-signal transducer and activator of transcription 3(STAT3)/STAT3 in skin tissue. The spleen was taken to detect the spleen index, and flow cytometry was employed to determine the expression of lymphocyte subsets. The results showed that group V had obvious pathological changes in skin tissue compared with group C. Moreover, group V showed more scratches, higher spleen index, and higher level of total serum IgE than group C. In addition, higher levels of p-JAK2 and p-STAT3, lower proportions of CD4~+T, Th1, and Treg, higher proportions of CD8~+T, Th2, and Th17, and lower ratios of CD4~+/CD8~+, Th1/Th2, and Terg/Th17 were observed in group V than in group C. Compared with group V, each administration group showed alleviation of the pathological morphology of skin tissue, obvious epidermal thickening, relatively intact collagen fiber structure of dermal reticular layer, alleviated edema, and relief of vasodilation and peripheral inflammatory cell infiltration. Moreover, less scratching, lower spleen index, lower p-JAK2/JAK2 and p-STAT3/STAT3 were observed in the administration groups than in group V. JF-M group and JF-H group demonstrated lower levels of total IgE, larger proportions of CD4~+T, Th1, and Treg, smaller proportions of CD8~+ T, Th2, and Th17, and higher ratios of CD4~+/CD8~+, Th1/Th2, and Terg/Th17. In conclusion, Jingfang Mixture may improve the symptoms of urticaria mice by regulating the balance of spleen T lymphocyte subsets through JAK2-STAT3 signaling pathway.
Xiang-Zi Li, Xi-Shuang Wang, Jian-Wei Fan, Tian-Ye Yang, Li-Juan Wang, Ying Sun, Jing-Chun Yao

2978 related Products with: [Jingfang Mixture regulates balance of spleen T lymphocyte subsets in urticaria mice by inhibiting JAK2-STAT3 signaling pathway].

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#36471327   2022/12/05 To Up

Similarities and differences between MIS-C and KD: a systematic review and meta-analysis.

Multisystem inflammatory syndrome in children (MIS-C) is a new syndrome with some clinical manifestations similar to Kawasaki disease (KD), which is difficult to distinguish.
Tong Tong, Xuefeng Yao, Zhe Lin, Yijing Tao, Jiawen Xu, Xiao Xu, Zhihao Fang, Zhimin Geng, Songling Fu, Wei Wang, Chunhong Xie, Yiying Zhang, Yujia Wang, Fangqi Gong

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1 mg25 mg10 mg10 mg1 mg2.5 mg100 mg100 mg25 mg50 mg

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#36469505   2022/12/05 To Up

The prevalence of SARS-CoV-2 antibodies within the community of a private tertiary university in the Philippines: A serial cross sectional study.

The COVID-19 pandemic has caused a public health emergency in all sectors of society, including universities and other academic institutions. This study determined the seroprevalence of SARS-CoV-2 antibodies among administrators, faculty, staff, and students of a private tertiary academic institution in the Philippines over a 7 month period. It employed a serial cross-sectional method using qualitative and quantitative COVID-19 antibody test kits. A total of 1,318 participants were tested, showing 47.80% of the study population yielding IgG antibodies to SARS-CoV-2 virus. A general increase in seroprevalence was observed from June to December 2021, which coincided with the vaccine roll-out of the country. All brands yielded positive antibody formation, with mRNA vaccines having higher levels than other types of vaccines. A decreasing trend in IgG reactivity was found in vaccinated individuals after 1 to 6 months of completion of the 2 doses of the COVID-19 vaccine. Where possible, IgG and T-cell reactivity and/or neutralizing capacity against SAR-CoV-2 need to be monitored regardless of vaccine brand. Together with uptake of COVID-19 vaccines and boosters, other public health interventions such as wearing of masks and regular testing need to be continued for better protection. Effective communication is also needed to inform risks associated with activities across different settings. Investments in long-term measures such as air filtration and ventilation systems, and wastewater surveillance need to be made.
Lourdes Bernadette C Sumpaico-Tanchanco, Jenica Clarisse Y Sy, Angel Belle C Dy, Myla Levantino, Arianna Maever L Amit, John Wong, Kirsten Angeles, John Paul C Vergara

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0.1 mg11 ml1 ml100

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#36469487   // To Up

Toxic Epidermal Necrolysis: A Review of Past and Present Therapeutic Approaches.

Toxic epidermal necrolysis (TEN) is an immune mediated, severe cutaneous adverse drug reaction characterized by epidermal detachment affecting greater than 30% body surface area. The mortality rate of TEN exceeds 20% and is usually caused by infection and respiratory compromise. Withdrawal of the causative drug, supportive care, and adjuvant therapy improve prognosis. Over the past decade, randomized controlled trials and meta-analyses have supported a role for cyclosporine, tumor necrosis factor alpha inhibitors, and combination therapy with intravenous immune globulin and corticosteroids. This review summarizes the medical management of TEN in adult patients.
Neha Singh, Mariana Phillips

1408 related Products with: Toxic Epidermal Necrolysis: A Review of Past and Present Therapeutic Approaches.

10 mg200ug10 mg50 ug100 mg100.00 ul100ug1,000 tests1 mg100ug

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#36469457   2022/12/05 To Up

Replenishment in the Family of Rhenium Chalcobromides; Synthesis and Structure of Molecular {ReS}Br(TeBr), Dimeric [{ReS}Br(TeBr)], and Polymeric {ReS}Br Compounds Based on the {ReS} Tetrahedral Cluster Core.

We have obtained three new rhenium(IV) chalcobromides belonging to the homologous series {ReS}Br(TeBr) ( = 0, 3, 4): a molecular complex {ReS}Br(TeBr) (), a dimeric complex [{ReS}(TeBr)Br(μ-Br)] (), and a two-dimensional (2D) polymeric compound {ReS}Br (). Compound is isotypic to the already known {ReTe}(TeBr)Br, while and exhibit a new type of binding of tetrahedral clusters via μ-Br bridges. Compounds were characterized by X-ray single-crystal diffraction, X-ray powder diffraction, and thermal and elemental analyses. In compound , two tetrahedral cluster cores {ReS} are linked together forming a dimer through two Re-μ-Br-Re bridges. Calculations of the electron localization function (ELF) showed that there is no covalent interaction between rhenium atoms of neighboring clusters. In compound , each rhenium atom of the {ReS} core is coordinated by three Br ligands: one terminal Br and two bridging μ-Br ligands. As a result, eight bridging bromine atoms link {ReS} cluster cores into goffered layers. {ReS}Br is the new stable rhenium(IV) thiobromide, the first discovered in the Re-S-Br system, along with the already known octahedral rhenium(III) thiobromides ReSBr ( = 0-4).
Spartak S Yarovoy, Mariia Ivanova, Taisiya S Sukhikh, Maxim R Ryzhikov, Vladimir E Fedorov, Nikolay G Naumov

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1100 mg25 mg

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