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Search results for: PKA (Protein Kinase A) Activity Assay kit

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#31632452   2019/10/11 To Up

Hepatic PKA inhibition accelerates the lipid accumulation in liver.

Liver lipid accumulation induced by high-fat diet (HFD) is an early onset process of non-alcoholic fatty liver diseases (NAFLD). Protein kinase A (PKA) is known to be involved in hepatic lipid metabolism. However, the role of PKA in NAFLD has not been well tested in vivo due to the lack of optimal PKA deficient mouse model.
Jining Yang, Xiaoying Zhang, Long Yi, Ling Yang, Wei Eric Wang, Chunyu Zeng, Mantian Mi, Xiongwen Chen

2119 related Products with: Hepatic PKA inhibition accelerates the lipid accumulation in liver.

48 assays96 wells

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#31629954   2019/10/17 To Up

Tambulin from Zanthoxylum armatum acutely potentiates the glucose-induced insulin secretion via K-independent Ca-dependent amplifying pathway.

Tambulin, a flavonol isolated from Zanthoxylum armatum, showed potent insulin secretory activity in our preliminary anti-diabetic screening. Here, we explored the insulin secretory mechanism(s) of tambulin focusing in glucose-dependent, K ‒ and Ca‒channels dependent, and cAMP-PKA pathways. Mice islets and MIN6 cells were incubated with tambulin in the presence of pharmacological agonists/antagonists and the secreted insulin was measured using mouse insulin ELISA kit. The intracellular cAMP was measured by an acetylation cAMP ELISA kit. Tambulin (200 μM) showed potent insulin secretory activity only at stimulatory glucose (11-25 mM) concentrations; however, no change in insulin release was observed at basal glucose both in mice islets and MIN6 cells. Notably, in the presence of diazoxide, a K channel opener; the incomplete inhibition of tambulin-induced insulin secretion was observed whereas, complete inhibition was found using verapamil, an L-type Ca channel blocker. Furthermore, the insulinotropic potential of tambulin was amplified in tolbutamide treated, and depolarized islets suggest tambulin's target other than tolbutamide. Tambulin showed no additive effect in the IBMX-induced intracellular cAMP; whereas, exerted an additive effect in the IBMX-induced insulin secretion. Furthermore, tambulin-induced insulin secretion was dramatically inhibited by PKA inhibitor (H-89), while moderate inhibition was found by using PKC inhibitor (calphostin C). Molecular docking studies also showed the best binding affinities of tambulin with PKA suggest the PKA dependent signaling cascade is involved more in tambulin-induced insulin secretion. Based on these findings, it is concluded that tambulin stimulates insulin secretion in a Ca channel-dependent but K channel-independent manner, most likely by activating the cAMP-PKA pathway.
Abdul Hameed, Sayed Ali Raza, M Israr Khan, Janaki Baral, Achyut Adhikari, Mohammad Nur-E-Alam, Sarfaraz Ahmed, Adnan J Al-Rehaily, Sajda Ashraf, Zaheer Ul-Haq, Rahman M Hafizur

1029 related Products with: Tambulin from Zanthoxylum armatum acutely potentiates the glucose-induced insulin secretion via K-independent Ca-dependent amplifying pathway.

2 Pieces/Box96 assays2 Pieces/Box2 Pieces/Box100ug Lyophilized2 Pieces/Box100.00 ug75мg/vial96 tests100ug100ug

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#30526586   2018/12/07 To Up

Extract of Paecilomyces hepiali mycelia induces lipolysis through PKA-mediated phosphorylation of hormone-sensitive lipase and ERK-mediated downregulation of perilipin in 3T3-L1 adipocytes.

Cordyceps sinensis has been used for centuries in China as one of the most valued herbal medicine and tonic food. Paecilomyces hepiali, a fungal strain isolated from natural C. sinensis, has been used widely as a substitute of C. sinensis in medicine and health food. P. hepiali has been reported to have various pharmaceutical benefits, including triglyceride-lowing activity. However, its effects on triglyceride metabolism in adipocytes remain unknown. The purpose of the present study was to evaluate the effect of P. hepiali mycelia on adipocyte lipolysis and to clarify the underlying mechanisms.
Mei Ge, Rui Guo, Hai-Xia Lou, Wen Zhang

2009 related Products with: Extract of Paecilomyces hepiali mycelia induces lipolysis through PKA-mediated phosphorylation of hormone-sensitive lipase and ERK-mediated downregulation of perilipin in 3T3-L1 adipocytes.

5 G250ul2 Pieces/Box400 ug2 Pieces/Box100 μg96 tests2 Pieces/Box2 Pieces/Box2 Pieces/Box2 Pieces/Box

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#29509826   2018/03/06 To Up

Enriched Chicken Bone Broth as a Dietary Supplement Reduces Nociception and Sensitization Associated with Prolonged Jaw Opening.

To test a commercially available enriched chicken bone broth (ECBB) product for its potential anti-inflammatory properties and to evaluate its ability to reduce nociception and expression of protein kinase A (PKA) in a clinically relevant model of temporomandibular disorder (TMD) caused by prolonged jaw opening in rats.
Jordan L Hawkins, Paul L Durham

1232 related Products with: Enriched Chicken Bone Broth as a Dietary Supplement Reduces Nociception and Sensitization Associated with Prolonged Jaw Opening.

500 grams900 tests50 ug105 μg5000 unit10 Plates1000 assays100tests25 assays100 assays200 assays

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#29506120   // To Up

Granulosa cells from human primordial and primary follicles show differential global gene expression profiles.

Can novel genetic candidates involved in follicle dormancy, activation and integrity be identified from transcriptomic profiles of isolated granulosa cells from human primordial and primary follicles?
E H Ernst, S Franks, K Hardy, P Villesen, K Lykke-Hartmann

1863 related Products with: Granulosa cells from human primordial and primary follicles show differential global gene expression profiles.

1000 1.00 flask5 x 50 ug100 100ul1mg1.00 flask100 100ul 100ul1.00 flask

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#26798992   2016/01/22 To Up

PAC1R agonist maxadilan enhances hADSC viability and neural differentiation potential.

Pituitary adenylate cyclase-activating polypeptide (PACAP) is a structurally endogenous peptide with many biological roles. However, little is known about its presence or effects in human adipose-derived stem cells (hADSCs). In this study, the expression of PACAP type I receptor (PAC1R) was first confirmed in hADSCs. Maxadilan, a specific agonist of PAC1R, could increase hADSC proliferation as determined by Cell Counting Kit-8 and cell cycle analysis and promote migration as shown in wound-healing assays. Maxadilan also showed anti-apoptotic activity in hADSCs against serum withdrawal-induced apoptosis based on Annexin V/propidium iodide analysis and mitochondrial membrane potential assays. The anti-apoptotic effects of maxadilan correlated with the down-regulation of Cleaved Caspase 3 and Caspase 9 as well as up-regulation of Bcl-2. The chemical neural differentiation potential could be enhanced by maxadilan as indicated through quantitative PCR, Western blot and cell morphology analysis. Moreover, cytokine neural redifferentiation of hADSCs treated with maxadilan acquired stronger neuron-like functions with higher voltage-dependent tetrodotoxin-sensitive sodium currents, higher outward potassium currents and partial electrical impulses as determined using whole-cell patch clamp recordings. Maxadilan up-regulated the Wnt/β-catenin signalling pathway associated with dimer-dependent activity of PAC1R, promoting cell viability that was inhibited by XAV939, and it also activated the protein kinase A (PKA) signalling pathway associated with ligand-dependent activity of PAC1R, enhancing cell viability and neural differentiation potential that was inhibited by H-89. In summary, these results demonstrated that PAC1R is present in hADSCs, and maxadilan could enhance hADSC viability and neural differentiation potential in neural differentiation medium.
Xiaoling Guo, Rongjie Yu, Ying Xu, Ruiling Lian, Yankun Yu, Zekai Cui, Qingshan Ji, Junhe Chen, Zhijie Li, Hongwei Liu, Jiansu Chen

1953 related Products with: PAC1R agonist maxadilan enhances hADSC viability and neural differentiation potential.

100ug100ug Lyophilized50 ug10 mg11 kit1000 tests0.1 mg200 100ug Lyophilized500 mg5000

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#26279440   2015/07/27 To Up

The Neuroprotective Effect of Liraglutide is Mediated by Glucagon-Like Peptide 1 Receptor-Mediated Activation of cAMP/PKA/CREB Pathway.

Glucagon-like peptide-1 (GLP-1)-based drugs are being used to achieve better glucose control in patients with type 2 diabetes, and GLP-1 mimetics such as liraglutide have shown therapeutic potential in preventing diabetes-related microvascular and macrovascular complications as well as comorbidities such as neurodegenerative disorders. In the present study, we investigated the effects of liraglutide on primary rat cortical astrocytes treated with advanced glycation end-products (AGEs).
Yi Bao, Lei Jiang, Haiyan Chen, Junjie Zou, Zhimin Liu, Yongquan Shi

1572 related Products with: The Neuroprotective Effect of Liraglutide is Mediated by Glucagon-Like Peptide 1 Receptor-Mediated Activation of cAMP/PKA/CREB Pathway.

100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized

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#25579542   // To Up

Carvedilol suppresses migration and invasion of malignant breast cells by inactivating Src involving cAMP/PKA and PKCδ signaling pathway.

Carvedilol (CAR) can inhibit cell growth and induce cell apoptosis in breast cancer in vitro. But it is still not known whether CAR affects the migration and invasion of breast cancer cells.
Gao Dezong, Ma Zhongbing, Fu Qinye, Yu Zhigang

2774 related Products with: Carvedilol suppresses migration and invasion of malignant breast cells by inactivating Src involving cAMP/PKA and PKCδ signaling pathway.

1.5 x 10^6 cells1.5x10(6) cells100ul500 mg200 25 mg1 ml100ug2 Pieces/Box10 mg25 mg 5 G

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#24977411   // To Up

Synthetic (+)-antroquinonol exhibits dual actions against insulin resistance by triggering AMP kinase and inhibiting dipeptidyl peptidase IV activities.

The fungal product (+)-antroquinonol activates AMP kinase (AMPK) activity in cancer cell lines. The present study was conducted to examine whether chemically synthesized (+)-antroquinonol exhibited beneficial metabolic effects in insulin-resistant states by activating AMPK and inhibiting dipeptidyl peptidase IV (DPP IV) activity.
C Y Hsu, R S Sulake, P-K Huang, H-Y Shih, H-W Sie, Y-K Lai, C Chen, C F Weng

2738 related Products with: Synthetic (+)-antroquinonol exhibits dual actions against insulin resistance by triggering AMP kinase and inhibiting dipeptidyl peptidase IV activities.

100 µg1 kit10 mU100μg 100ul100μg100μg96 wells (1 kit)1 kit2.5 mg100ug100 ul

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