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#33869946   2021/04/01 To Up

Development of a Nucleocapsid Protein-Based ELISA for Detection of Human IgM and IgG Antibodies to SARS-CoV-2.

SARS-CoV-2 is the etiologic agent of COVID-19, which has led to a dramatic loss of human life and presents an unprecedented challenge to public health worldwide. The gold standard assay for SARS-CoV-2 identification is real-time polymerase chain reaction; however, this assay depends on highly trained personnel and sophisticated equipment and may suffer from false results. Thus, a serological antibody test is a supplement to the diagnosis or screening of SARS-CoV-2. Here, we develop and evaluate the diagnostic performance of an IgM/IgG indirect ELISA method for antibodies against SARS-CoV-2 in COVID-19. The ELISA was constructed by coating with a recombinant nucleocapsid protein of SARS-CoV-2 on an enzyme immunoassay plate, and its sensitivity and specificity for clinical diagnosis of SARS-CoV-2 infection was assessed by detecting the SARS-CoV-2-specific IgM and IgG antibodies in COVID-19 patient's sera or healthy person's sera. The SARS-CoV-2 positive serum samples ( = 168) were collected from confirmed COVID-19 patients. A commercial nucleocapsid protein-based chemiluminescent immunoassay (CLIA) kit and a colloidal gold immunochromatography kit were compared with those of the ELISA assay. The specificity, sensitivity, positive predictive value (PPV), and negative predictive value (NPV) of IgM were 100, 95.24, 100, and 91.84%, whereas those of IgG were 100, 97.02, 100, and 94.74%, respectively. We developed a highly sensitive and specific SARS-CoV-2 nucleocapsid protein-based ELISA method for the diagnosis and epidemiologic investigation of COVID-19 by SARS-CoV-2 IgM and IgG antibody detection.
Pan-Pan Liu, Yang Zong, Shu-Peng Jiang, Yong-Jun Jiao, Xue-Jie Yu

1332 related Products with: Development of a Nucleocapsid Protein-Based ELISA for Detection of Human IgM and IgG Antibodies to SARS-CoV-2.

50 ug Product tipe: Antib100 50 ug Product tipe: Antib100100 μg0.5 mg100 1 mg0.1mg100 TESTS500 100 μg

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#33860008   2021/03/05 To Up

Isolation and molecular detection of from popped eye disease of cultured Tilapia and Vietnamese koi fishes in Bangladesh.

Present research aims to isolate, identify, and determine the virulence of the (group B ; GBS), isolated from popped eye disease affected Tilapia and Vietnamese Koi (V. Koi) fishes.
Mohummad Muklesur Rahman, Md Ashikur Rahman, Md Shirajum Monir, Md Enamul Haque, Mahbubul Pratik Siddique, A K M Khasruzzaman, Md Tanvir Rahman, Md Alimul Islam

1529 related Products with: Isolation and molecular detection of from popped eye disease of cultured Tilapia and Vietnamese koi fishes in Bangladesh.

100 mg10 mg100ug96 tests10 mg500 tests2.5 mg

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#33728953   2021/03/17 To Up

First report of causing stem canker on L. in Oregon.

In August of 2020, plants of L. grown in hoop houses at two farms located in Benton County, Oregon exhibited wilting and chlorosis, followed by shoot necrosis. Symptomatic plants had dry, tan-brown lesions or cankers, often accompanied by large, round to irregular or ribbon-shaped, black sclerotia and/or profuse white mycelial growth. Lesions or cankers were observed on the stems at both the plant crown (soil) level and higher in the canopy; flower infections were not observed. Sclerotia were removed from two infected plants and placed on potato dextrose agar (PDA) at room temperature. Fast-growing, pure white, largely appressed, sterile mycelium grew radially from plated sclerotia. Hyphal tips were transferred to obtain a pure culture. Additional sclerotia, solitary and aggregate, approximately 30 to more than 50 per plate, exhibiting identical features to those observed on plant tissue, formed in culture 6-7 days following transfer and ranged in size from 2 to 11 mm in length or width (n=50). Mycelia were aseptically harvested from cultures for DNA extraction (-DNA Plant/Seed Miniprep Kit, Zymo Research). Primers ITS1-F (Gardes and Bruns 1993) and ITS4 (White et al. 1990) were used to amplify the internal transcribed spacer region (ITS) and primers G3PDHfor and G3PDHrev were used to amplify the glyceraldehyde 3-phosphate dehydrogenase (G3PDH) gene (Staats et al. 2005) from a single isolate, LAS01. The ITS region from LAS01 (MW079844) shared 100 to >99% homology to several species isolates in GenBank. The LAS01 G3PDH gene (MW082601), shared >99% and 100% homology with type specimens strains 484 (GenBank accession no. AJ705044) and 1980 (JQ036048), respectively, and only 97% and 96% sequence identity with (KF878364) and (KF878375), respectively. A phylogenetic tree (presented as an eXtra) identifies LAS01 as . To confirm pathogenicity, isolate LAS01 was grown on PDA at room temperature. After 48 hours, 4mm plugs were cut from the colony and placed mycelium-side down onto the main stems of five healthy plants that had been grown for approximately six weeks from rooted cuttings and secured using a minutien pin. Uncolonized PDA plugs placed on the stem of the same plants several leaf nodes away were used as controls. Plants were incubated at room temperature in a grow tent under 24-hour light and 70-95% humidity conditions. Elongate, tan-brown lesions were observed at the inoculation sites 4-5 days post inoculation; stems at mock inoculated sites remained green. After six days, tissue was excised from the margin of each lesion, surface sterilized with 1% NaOCl, rinsed in sterile water, and placed onto PDA. Resultant fungal growth was confirmed to be based on morphology. Isolation attempts were also made from mock inoculations; no fungal growth was observed. Trials were repeated on two additional cultivars with similar results. This report is the first of on in Oregon; the only peer-reviewed reports that could be located for on in the United States were from host indices in Montana (Anon. 1960; Shaw 1973) and references cited by McPartland (1996). has been reported in Canada on hemp-type (Bains et al. 2000). The economic impact of on the emerging industry in Oregon and the United States remains unclear.
Andrea Garfinkel

1462 related Products with: First report of causing stem canker on L. in Oregon.

96 tests96 wells3 inhibitors1 mg10 ug100ug100ug Lyophilized100 μg100 μg

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#33712666   2021/03/12 To Up

Photooxidation-induced fluorescence amplification system for an ultra-sensitive enzyme-linked immunosorbent assay (ELISA).

This report suggests a method of enhancing the sensitivity of chemifluorescence-based ELISA, using photooxidation-induced fluorescence amplification (PIFA). The PIFA utilized autocatalytic photooxidation of the chemifluorescent substrate, 10-acetyl 3,7-dihydroxyphenoxazine (ADHP, Amplex Red) to amplify the fluorescent product resorufin, initially oxidized by horse radish peroxidase (HRP). As the amplification rate is proportional to the initial level of resorufin, the level of antigen labeled by HRP is quantified by analyzing the profile of fluorescence intensity. The normalized profile was interpolated into an autocatalysis model, and the rate of increase at half-maximum time was quantified by the use of an amplification index (AI). The lower limit of detection, for resorufin or HRP, was less than one-tenth that of the plate reader. It requires only slight modification of the fluorescence reader and is fully compatible with conventional or commercial ELISA. When it is applied to a commercial ELISA kit for the detection of amyloid beta, it is verified that the PIFA assay enhanced the detection sensitivity by more than a factor of 10 and was compatible with a conventional 96-well ELISA assay kit. We anticipate this PIFA assay to be used in research for the detection of low levels of proteins and for the early diagnosis of various diseases with rare protein biomarkers, at ultra-low (pg/mL) concentrations.
Youhee Heo, Kwanwoo Shin, Min Cheol Park, Ji Yoon Kang

2847 related Products with: Photooxidation-induced fluorescence amplification system for an ultra-sensitive enzyme-linked immunosorbent assay (ELISA).

1 kit1 kit1 kit1 kit1 kit(96 Wells)1 kit96 wells (1 kit)1 kit1 kit96T

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#33654408   2021/02/22 To Up

miR-744-5p Inhibits Multiple Myeloma Proliferation, Epithelial Mesenchymal Transformation and Glycolysis by Targeting SOX12/Wnt/β-Catenin Signaling.

This study investigated the function and molecular mechanisms of miR-744-5p in multiple myeloma (MM).
Bingling Guo, Chunyan Xiao, Yumin Liu, Ning Zhang, Hao Bai, Tao Yang, Ying Xiang, Yingyu Nan, Qiying Li, Wenjun Zhang, Dehong Huang

2403 related Products with: miR-744-5p Inhibits Multiple Myeloma Proliferation, Epithelial Mesenchymal Transformation and Glycolysis by Targeting SOX12/Wnt/β-Catenin Signaling.

16 Arrays/Slide100ug2500 assays16 Arrays/Slide100.00 ul

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#33420120   2021/01/08 To Up

A common protocol for the simultaneous processing of multiple clinically relevant bacterial species for whole genome sequencing.

Whole-genome sequencing is likely to become increasingly used by local clinical microbiology laboratories, where sequencing volume is low compared with national reference laboratories. Here, we describe a universal protocol for simultaneous DNA extraction and sequencing of numerous different bacterial species, allowing mixed species sequence runs to meet variable laboratory demand. We assembled test panels representing 20 clinically relevant bacterial species. The DNA extraction process used the QIAamp mini DNA kit, to which different combinations of reagents were added. Thereafter, a common protocol was used for library preparation and sequencing. The addition of lysostaphin, lysozyme or buffer ATL (a tissue lysis buffer) alone did not produce sufficient DNA for library preparation across the species tested. By contrast, lysozyme plus lysostaphin produced sufficient DNA across all 20 species. DNA from 15 of 20 species could be extracted from a 24-h culture plate, while the remainder required 48-72 h. The process demonstrated 100% reproducibility. Sequencing of the resulting DNA was used to recapitulate previous findings for species, outbreak detection, antimicrobial resistance gene detection and capsular type. This single protocol for simultaneous processing and sequencing of multiple bacterial species supports low volume and rapid turnaround time by local clinical microbiology laboratories.
Kathy E Raven, Sophia T Girgis, Asha Akram, Beth Blane, Danielle Leek, Nicholas Brown, Sharon J Peacock

2536 related Products with: A common protocol for the simultaneous processing of multiple clinically relevant bacterial species for whole genome sequencing.

0.2 mg100 ml0.1 mg100ug1 ml25 µg 100ul0.2 mg 100ul25 µg0.25 mg

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#33374317   2020/12/24 To Up

Development of Fluorescence In Situ Hybridization as a Rapid, Accurate Method for Detecting Coliforms in Water Samples.

Coliform bacteria are indicators of water quality; however, most detection methods for coliform bacteria are time-consuming and nonspecific. Here, we developed a fluorescence in situ hybridization (FISH) approach to detect four types of coliform bacteria, including , , and , simultaneously in water samples using specific probes for 16S rRNA. This FISH method was applied to detect coliform bacteria in simulated water and domestic wastewater samples and compared with traditional detection methods (e.g., plate counting, multiple-tube fermentation (MTF) technique, and membrane filter (MF) technique). Optimal FISH conditions for detecting the four types of coliforms were found to be fixation in 3% paraformaldehyde at 4 °C for 2 h and hybridization at 50 °C for 1.5 h. By comparing FISH with plate counting, MTF, MF, and a commercial detection kit, we found that FISH had the shortest detection time and highest accuracy for the identification of coliform bacteria in simulated water and domestic wastewater samples. Moreover, the developed method could simultaneously detect individual species and concentrations of coliform bacteria. Overall, our findings indicated that FISH could be used as a rapid, accurate biosensor system for simultaneously detecting four types of coliform bacteria to ensure water safety.
Jong-Tar Kuo, Li-Li Chang, Chia-Yuan Yen, Teh-Hua Tsai, Yu-Chi Chang, Yu-Tang Huang, Ying-Chien Chung

1717 related Products with: Development of Fluorescence In Situ Hybridization as a Rapid, Accurate Method for Detecting Coliforms in Water Samples.

400Tests1 kit1 kit48 samples1 kit1 kit96 samples0.1ml (1mg/ml)96 samples1 kit96 samples96 samples

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#33117324   2020/09/29 To Up

Effect of Environmental Conditions on the Formation of the Viable but Nonculturable State of BM-PA17927 and Its Control and Detection in Food System.

: This study aimed to investigate the effect of environmental conditions including nutrient content, acetic acid concentration, salt concentration, and temperature on the formation of viable but nonculturable (VBNC) state of , as well as its control and detection in food system. : Representing various environmental conditions in different food systems, 16 induction groups were designed for the formation of VBNC state of . Traditional plate counting was applied to measure the culturable cell numbers, and Live/Dead Bacterial Viability Kit combined with fluorescent microscopy was used to identify viable cells numbers. The inhibition of bacterial growth and VBNC state formation by adjusting the environmental conditions were investigated, and the clearance effect of VBNC cells in crystal cake system was studied. In addition, a propidium monoazide-polymerase chain reaction (PMA-PCR) assay was applied to detect the VBNC cells in crystal cake food system. : Among the environmental conditions included in this study, acetic acid concentration had the greatest effect on the formation of VBNC state of , followed by nutritional conditions and salt concentration. Reducing nutrients in the environment and treating with 1.0% acetic acid can inhibit from entering the VBNC state. In the crystal cake system, the growth of and the formation of VBNC state can be inhibited by adding 1.0% acetic acid and storing at -20°C. In crystal cake system, the PMA-PCR assay can be used to detect VBNC cells at a concentration higher than 10 cells/ml. : The VBNC state of can be influenced by the changing of environmental conditions, and PMA-PCR assay can be applied in food system for the detection of VBNC cells.
Yanmei Li, Teng-Yi Huang, Yuzhu Mao, Yanni Chen, Fan Shi, Ruixin Peng, Jinxuan Chen, Caiying Bai, Ling Chen, Kan Wang, Junyan Liu

1777 related Products with: Effect of Environmental Conditions on the Formation of the Viable but Nonculturable State of BM-PA17927 and Its Control and Detection in Food System.

5 G125 mg

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