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Search results for: Total Antioxidant Capacity (TAC) Assay Kit100 assays

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#32545290   2020/06/11 To Up

One-Pot Multicomponent Synthesis and Bioevaluation of Tetrahydroquinoline Derivatives as Potential Antioxidants, α-Amylase Enzyme Inhibitors, Anti-Cancerous and Anti-Inflammatory Agents.

Mankind has always suffered from multiple diseases. Therefore, there has been a rigorous need in the field of medicinal chemistry for the design and discovery of new and potent molecular entities. In this work, thirteen tetrahydroquinoline derivatives were synthesized and evaluated biologically for their antioxidant, α-amylase enzyme inhibitory, anti-proliferative and anti-inflammatory activities. showed the lowest IC of 29.19 ± 0.25 µg/mL by scavenging DPPH free radicals. showed significant antioxidant activity in total antioxidant capacity (TAC) and total reducing power (TRP) assays. and showed the maximum inhibition of α-amylase enzyme i.e., 97.47% and 89.93%, respectively, at 200 µg/mL concentration. Five compounds were shortlisted to determine their anti-proliferative potential against Hep-2C cells. The study was conducted for 24, 48 and 72 h. showed significant results, having an IC value of 11.9 ± 1.04 µM at 72 h when compared with standard cisplatin (IC value of 14.6 ± 1.01 µM). An in vitro nitric oxide (NO) assay was performed to select compounds for in vivo anti-inflammatory activity evaluation. scavenged the NO level to a maximum of 85% at 50 µM concentration, followed by and . Based on the NO scavenging assay results, in vivo anti-inflammatory studies were also performed and the results showed significant activity compared to the standard, acetylsalicylic acid (ASA).
Samra Farooq, Aqsa Mazhar, Areej Ghouri, Ihsan-Ul-Haq, Naseem Ullah

1842 related Products with: One-Pot Multicomponent Synthesis and Bioevaluation of Tetrahydroquinoline Derivatives as Potential Antioxidants, α-Amylase Enzyme Inhibitors, Anti-Cancerous and Anti-Inflammatory Agents.

0.1 mg1 ml96 wells100ul200 1000 TESTS/0.65ml100ul1000 100.00 ul100ug Lyophilized100μg100ug Lyophilized

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#32514483   2020/06/02 To Up

Effects of growth stage and seasons on the phytochemical content and antioxidant activities of crude extracts of

This study investigated the phytochemical constituents and antioxidant properties of crude extracts of at different maturity stages and seasons. Total phenols, flavonoids, and proanthocyanidin content from water, acetone and methanol extracts were evaluated spectrophotometrically. The antioxidant activities were measured using 2,2- diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) (ABTS), Ferric reducing antioxidant power (FRAP) and Total antioxidant capacity (TAC) models. Results showed that the flowering stages in all the solvent extracts gave the highest polyphenolic content with the acetone extract significantly higher than the methanol and aqueous extracts (P < 0.05). The highest value for total polyhenolic content 80.75 ± 4.21 for the first trial and 89.69 ± 2.13 μg/mL in the second trial; while the flavonoids was 874.76 ± 7.87 and 946.19 ± 7.87 μg/mL in the first and second trials respectively; and proanthocyanidin content was 170.00 ± 0 and 100.90 ± 1.29 μg/mL. Overall, the aqueous extracts had the lowest content of all the phytochemicals. The antioxidant activities ranged from low to high at different growth stages of the plant. While low to no activity was observed in the aqueous extracts in all the assays, the methanol extracts of the flowering stages showeds the best activity in the first and second trials with IC values of 104.10 ± 8.59 and 120.02 ± 13.37 μg/mL respectively in ABTS. Similar trend was obtained in the DPPH assay with the highest activity in the methanol flowering extract with IC of 52.36 ± 0.76 μg/mL (first trial) and 49.36 ± 0.29 μg/mL (second trial). The FRAP and TAC also had the highest activity in the flowering stages in all solvents, but with the acetone extracts having the overall inhibition on both radicals. This study revealed that phytoconsituents and antioxidant potential can be influenced by physiological and developmental stages of the plant.
O D Adegbaju, G A Otunola, A J Afolayan

1943 related Products with: Effects of growth stage and seasons on the phytochemical content and antioxidant activities of crude extracts of

5 G500 mg25 mg10 mg100ul25 mg 5 G 5 G200ug50 ug 1 g96 wells (1 kit)

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#32479004   // To Up

Antioxidant and Genotoxic Effects of Aqueous and Methanol Extracts from Two Edible Mushrooms from Turkey in Human Peripheral Lymphocytes.

This study reported the genetic and oxidative effects of aqueous and methanol extracts from two edible mushrooms, Lepista nuda (Bull.) Cooke and Pleurotus ostreatus (Jacq.) P. Kummer, in cultured human lymphocytes. Chromosome aberration (CA) and micronucleus (MN) assays were used for genotoxic influences estimation. In addition, the changes of total antioxidant capacity (TAC) and total oxidative stress (TOS) in the cells were monitored. The fungal extracts at all applied concentrations did not indicate significant differences (p > 0.05) in CA and MN analyses. Furthermore, while the treatments with maximum concentration of aqueous extract of L. nuda statistically (p < 0.05) increased TAC especially, TOS levels in the cells were reduced by them in comparison with negative control. Based on TAC analysis, low IC50 values belonging to aqueous (5.43 mg/L) and methanol (10.88 mg/L) extracts of L. nuda were remarkable. Our data demonstrated that the extracts obtained from P. ostreatus and especially L. nuda can be a new resource for therapeutics with their nonmutagenic and antioxidant features.
Bugrahan Emsen, Busranur Guven, Yasin Uzun, Abdullah Kaya

2173 related Products with: Antioxidant and Genotoxic Effects of Aqueous and Methanol Extracts from Two Edible Mushrooms from Turkey in Human Peripheral Lymphocytes.

200 200 200 1000 0.1 mg100ul1 ml100 μg 100ul100 μg100ug

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#32456941   2020/03/24 To Up

Determination of total antioxidant capacity of Cynara Scolymus L. (globe artichoke) by using novel nanoparticle-based ferricyanide/Prussian blue assay.

A novel ferricyanide/Prussian blue (PB) assay for total antioxidant capacity (TAC) determination was developed exploiting the formation of PB nanoparticles in the presence of polyvinylpyrrolidone (PVP) as stabilizer. This improved method, named as "nanoparticle-based ferricyanide/Prussian blue assay (PBNP)", was applied to the TAC measurement of Cynara Scolymus L. (globe artichoke). The calibration results of the novel (PBNP) method were compared with those of a similar nanoparticle PB method performed in the absence of PVP, and of a sodium dodecyl sulfate-modified and acid-optimized ferricyanide reference assay. Compared to similar common Fe(III)-based TAC assays, much higher molar absorptivities, pointing out higher response to different kinds of antioxidants, were obtained with PBNP for all tested antioxidants, and lower LOD and LOQ values were achieved for thiols. As an additional advantage, methionine, not responding to other electron-transfer based TAC reagents, could be measured. PBNP could detect various antioxidants with one-two orders-of-magnitude lower LOD values than those of widely used TAC assays like CUPRAC and Folin-Ciocalteau well correlating with the proposed assay.
Nergiz Kanmaz, Ayşem Uzer, Jülide Hizal, Resat Apak

1463 related Products with: Determination of total antioxidant capacity of Cynara Scolymus L. (globe artichoke) by using novel nanoparticle-based ferricyanide/Prussian blue assay.

96100 assays100 assays100tests430 tests100tests1 kit(96 Wells)100tests500 tests1 kit100 assays

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#32352878   2020/05/13 To Up

UHPLC-MS phytochemical profiling, biological propensities and studies of roots: a medicinal herb with multifunctional properties.

The biological, chemical, and properties of methanol and dichloromethane (DCM) extracts of roots with respect to the antioxidant, enzyme inhibition, and phytochemical composition were evaluated. Total bioactive contents were determined spectrophotometrically, and the individual secondary metabolites composition was assessed ultra-high-performance liquid chromatography mass spectrometry (UHPLC-MS) analysis. Antioxidant capacities were evaluated using a panoply of assays (2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) free radical scavenging, ferric reducing antioxidant power (FRAP), cupric reducing antioxidant power (CUPRAC), phosphomolybdenum total antioxidant capacity (TAC), and metal chelating activity (MCA)). The enzyme inhibition potential was studied against acetylcholinesterase (AChE), butyrylcholinesterase (BChE), α-amylase, α-glucosidase, tyrosinase, urease and lipoxygenase (LOX) enzymes. The methanol extract was found to contain higher total phenolic (105.91 mg GAE/g extract) and flavonoid (2.27 mg RE/g extract) contents which can be correlated to its more substantial antioxidant potential as well as AChE, BChE, tyrosinase and α-glucosidase inhibition. However, the DCM extract was the most effective against α-amylase (1.86 mmol ACAE/g extract) enzyme inhibition. The UHPLC-MS analysis of methanol extract identified the tentative presence of a total of 18 secondary metabolites, including flavonoids, saponins, phenolic and terpenoid derivatives. Three compounds named emmotin A, luteolin 5,3'-dimethyl ether, and preferrugone were further investigated for their molecular docking studies against the tested enzymes. The selected compounds were found to have higher binding interaction with AChE followed by BChE, α-glucosidase, α-amylase, and tyrosinase. The results of the present study have demonstrated to be considered as a lead source of natural antioxidant and enzyme inhibitor compounds.
Hammad Saleem, Muhammad Sarfraz, Kashif Maqbool Khan, Muhammad Aijaz Anwar, Gokhan Zengin, Irshad Ahmad, Shafi-Ullah Khan, Mohamad Fawzi Mahomoodally, Nafees Ahemad

1879 related Products with: UHPLC-MS phytochemical profiling, biological propensities and studies of roots: a medicinal herb with multifunctional properties.

100ug Lyophilized10ml10 mg10 ug0.1 mg1 Set2.5 mg100ug1 ml6200ul

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#32215836   2020/03/25 To Up

Neuroprotective effects of boron nitride nanoparticles in the experimental Parkinson's disease model against MPP+ induced apoptosis.

Parkinson's disease (PD) is one of the most aggressive neurodegenerative diseases and characterized by the loss of dopamine-sensitive neurons in the substantia nigra region of the brain. There is no any definitive treatment to completely cure PD and existing treatments can only ease the symptoms of the disease. Boron nitride nanoparticles have been extensively studied in nano-biological studies and researches showed that it can be a promising candidate for PD treatment with its biologically active unique properties. In the present study, it was aimed to investigate ameliorative effects of hexagonal boron nitride nanoparticles (hBNs) against toxicity of 1-methyl-4-phenylpyridinium (MPP) in experimental PD model. Experimental PD model was constituted by application of MPP to differentiated pluripotent human embryonal carcinoma cell (Ntera-2, NT-2) culture in wide range of concentrations (0.62 to 2 mM). Neuroprotective activity of hBNs against MPP toxicity was determined by cell viability assays including MTT and LDH release. Oxidative alterations by hBNs application in PD cell culture model were investigated using total antioxidant capacity (TAC) and total oxidant status (TOS) tests. The impacts of hBNs and MPP on nuclear integrity were analyzed by Hoechst 33258 fluorescent staining method. Acetylcholinesterase (AChE) enzyme activities were determined by a colorimetric assay towards to hBNs treatment. Cell death mechanisms caused by hBNs and MPP+ exposure was investigated by flow cytometry analysis. Experimental results showed that application of hBNs increased cell viability in PD model against MPP application. TAS and TOS analysis were determined that antioxidant capacity elevated after hBNs applications while oxidant levels were reduced. Furthermore, flow cytometric analysis executed that MPP induced apoptosis was prevented significantly (p < 0.05) after application with hBNs. In a conclusion, the obtained results indicated that hBNs have a huge potential against MPP toxicity and can be used in PD treatment as novel neuroprotective agent and drug delivery system.
Recep Küçükdoğru, Hasan Türkez, Mehmet Enes Arslan, Özlem Özdemir Tozlu, Erdal Sönmez, Adil Mardinoğlu, Ivana Cacciatore, Antonio Di Stefano

1446 related Products with: Neuroprotective effects of boron nitride nanoparticles in the experimental Parkinson's disease model against MPP+ induced apoptosis.

96 tests1-99 mg/ml/ea price x 2500 tests96T12 Pieces/Box

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#32041310   2020/02/06 To Up

Antioxidant and Anti-Proliferative Properties of Roots and Their Potentially Active Components.

(Bruce) J. F. Gmel. is a multipurpose dioecious tree that has been used to treat various ailments, for example, the flowers of have been widely used as a tea to treat intestinal parasites by local residents and the roots of H. abyssinica could also be used for anticancer purposes. Antioxidant activity could be one of the most important pathways to suppress cancer and there is hardly any information available on the specific chemical components corresponding to the bioactivities of to date. The present study intended to screen and evaluate the antioxidant and anti-proliferative properties of five different fractions from along with their corresponding total flavonoid and phenolic contents and then further identify those compounds with the most potent antioxidant and anti-proliferative activities using high performance liquid chromatography (HPLC) coupled to mass spectrometry (MS) and nuclear magnetic resonance (NMR). The total flavonoid and phenolic content assays showed that the ethyl acetate (EA) fraction of had higher flavonoid and phenolic levels than the other four fractions. Furthermore, the 2,2-diphenyl-1-picrylhydrazyl (DPPH) superoxide radical scavenging abilities, total antioxidant capacity (TAC) assay with 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid (ABTS), and ferric-reducing antioxidant power (FRAP) were measured to evaluate the antioxidant activities of the five fractions and some pure compounds isolated from the EA fraction, which displayed higher antioxidant properties than that of the other fractions. Caffeic acid from the EA fraction showed even stronger DPPH scavenging ability (IC 7.858 ± 0.31 µg/mL) than that of Vc (IC 8.27 ± 0.11 µg/mL) as the positive control. The anti-proliferative properties of four fractions and the ethanol extract were evaluated by the 3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-di-phenytetrazoliumromide (MTT) assay and the EA fraction exhibited higher anti-proliferative activities against three cancer cell lines than that of the other fractions. Additionally, the compounds with good antioxidant activity from the EA fraction of were screened and identified using LC-MS and NMR and were also found to possess good anti-proliferative activity. In the MTT assay, the quercetin showed the strongest dose-dependent anti-proliferative activities to colon cancer cells (HT-29) and liver cancer cells (HepG2) among all of the compounds isolated. This study provided valuable information on the synergistic antioxidant and anti-proliferative properties of .
Minxia Fan, Guilin Chen, Yongli Zhang, Lutfun Nahar, Satyajit Dey Sarker, Guangwan Hu, Mingquan Guo

2047 related Products with: Antioxidant and Anti-Proliferative Properties of Roots and Their Potentially Active Components.

1 ml100.00 ul1000 TESTS/0.65ml200 100ul0.1 mg1000 100ul10 mg10 mg 5 G

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#32002976   // To Up

Association of Paraoxonse1 (PON1) Genotypes with the Activity of PON1 in Patients with Parkinson's Disease.

Various numbers of factors such as oxidative stress, neurotoxins, and pesticides have been implicated in its pathophysiology of Parkinson's disease (PD). Paraoxonas1 (PON1) metabolizes xenobiotics, including pesticides. Therefore, we surveyed the relationship between PON1 polymorphisms with its activities in the pathogenesis of Parkinson's disease..
Ali Mota, Mohsen Hemati-Dinarvand, Ali Akbar Taheraghdam, Hamid Reza Nejabati, Ravan Ahmadi, Tohid Ghasemnejad, Milad Hasanpour, Mohammad Valilo

2263 related Products with: Association of Paraoxonse1 (PON1) Genotypes with the Activity of PON1 in Patients with Parkinson's Disease.

400Tests1-99 mg/ml/ea price x 296 tests

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#31939585   // To Up

Effects of intra-dentate gyrus microinjection of myokine irisin on long-term potentiation in male rats.

Induction of long-term potentiation (LTP) increases the storage capacity of synapses in the hippocampal dentate gyrus (DG). Irisin is a myokine generated from FNDC5 (a gene precursor) during exercise.
Saeed Mohammadi, Shahrbanoo Oryan, Alireza Komaki, Akram Eidi, Mohammad Zarei

2294 related Products with: Effects of intra-dentate gyrus microinjection of myokine irisin on long-term potentiation in male rats.

100 μg 0.1 mg 100 μg100 μg100 μg100 μg100 μg100 μg100 μg50 100 μg

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#31935857   2020/01/06 To Up

The Link between Polyphenol Structure, Antioxidant Capacity and Shelf-Life Stability in the Presence of Fructose and Ascorbic Acid.

Polyphenols play an important role in the sensorial and health-promoting properties of fruits and vegetables and display varying structure-dependent stability during processing and shelf-life. The current work aimed to increase the fundamental understanding of the link between the stability of polyphenols as a function of their structure, presence of ascorbic acid and fructose and total antioxidant capacity (TAC), using a multi-component model system. Polyphenol extract, used as the multi-component model system, was obtained from freeze-dried, high polyphenol content strawberry ( 'Nerina') and twenty-one compounds were identified using high-performance liquid chromatography-mass spectrometry (HPLC-MS). The TAC and the first-order degradation kinetics were obtained, linking the polyphenol stability to its chemical structure, with and without the presence of fructose and ascorbic acid. The TAC (measured by oxygen radical absorption capacity (ORAC) and ferric reducing antioxidant potential (FRAP) assays) was not dramatically affected by storage temperatures and formulation, while polyphenol stability was significantly and structure dependently affected by temperature and the presence of ascorbic acid and fructose. Anthocyanins and phenolic acids were more unstable in the presence of ascorbic acid, while flavonol stability was enhanced by its presence. Shelf life study performed at 37 °C revealed significantly higher stability of purified polyphenols vs. the stability of the same polyphenols in the strawberry extract (multi-component system).
Inbal Hanuka Katz, Eden Eran Nagar, Zoya Okun, Avi Shpigelman

2020 related Products with: The Link between Polyphenol Structure, Antioxidant Capacity and Shelf-Life Stability in the Presence of Fructose and Ascorbic Acid.

10 mg100tests100tests1

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