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Search results for: Mitochondrial DNA Isolation Kit50 assays

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#33125422   2020/10/30 To Up

Sample adequacy controls for infectious disease diagnosis by oral swabbing.

Oral swabs are emerging as a non-invasive sample type for diagnosing infectious diseases including Ebola, tuberculosis (TB), and COVID-19. To assure proper sample collection, sample adequacy controls (SACs) are needed that detect substances indicative of samples collected within the oral cavity. This study evaluated two candidate SACs for this purpose. One detected representative oral microbiota (Streptococcus species DNA) and the other, human cells (human mitochondrial DNA, mtDNA). Quantitative PCR (qPCR) assays for the two target cell types were applied to buccal swabs (representing samples collected within the oral cavity) and hand swabs (representing improperly collected samples) obtained from 51 healthy U.S. volunteers. Quantification cycle (Cq) cutoffs that maximized Youden's index were established for each assay. The streptococcal target at a Cq cutoff of ≤34.9 had 99.0% sensitivity and specificity for oral swab samples, whereas human mtDNA perfectly distinguished between hand and mouth swabs with a Cq cutoff of 31.3. The human mtDNA test was then applied to buccal, tongue, and gum swabs that had previously been collected from TB patients and controls in South Africa, along with "air swabs" collected as negative controls (total N = 292 swabs from 71 subjects). Of these swabs, 287/292 (98%) exhibited the expected Cq values. In a paired analysis the three oral sites yielded indistinguishable amounts of human mtDNA, however PurFlockTM swabs collected slightly more human mtDNA than did OmniSwabsTM (p = 0.012). The results indicate that quantification of human mtDNA cannot distinguish swabs collected from different sites within the mouth. However, it can reliably distinguish oral swabs from swabs that were not used orally, which makes it a useful SAC for oral swab-based diagnosis.
Meagan Deviaene, Kris M Weigel, Rachel C Wood, Angelique K K Luabeya, Lisa Jones-Engel, Mark Hatherill, Gerard A Cangelosi

1144 related Products with: Sample adequacy controls for infectious disease diagnosis by oral swabbing.

500 tests500 tests1-99 mg/ml/ea price x 21 mg96T500 tests500 tests500 tests1-99 mg/ml/ea price x 2500 tests

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#32826930   2020/08/21 To Up

A newly isolated strain of Halomonas sp. (HA1) exerts anticancer potential via induction of apoptosis and G/M arrest in hepatocellular carcinoma (HepG2) cell line.

Marine bacterial strains are of great interest for their ability to produce secondary metabolites with anticancer potentials. Isolation, identification, characterization and anticancer activities of isolated bacteria from El-Hamra Lake, Wadi El-Natrun (Egypt) were the objectives of this study. The isolated bacteria were identified as a moderately halophilic alkaliphilic strain. Ethyl acetate extraction was performed and identified by liquid chromatography-mass spectrophotometry (LC-MS-MS) and nuclear magnetic resonance analysis (NMR). Cytotoxicity of the extract was assessed on the HepG2 cell line and normal human peripheral lymphocytes (HPBL) in vitro. Halomonas sp. HA1 extract analyses revealed anticancer potential. Many compounds have been identified including cyclo-(Leu-Leu), cyclo-(Pro-Phe), C17-sphinganine, hexanedioic acid, bis (2-ethylhexyl) ester, surfactin C14 and C15. The extract exhibited an IC of 68 ± 1.8 μg/mL and caused marked morphological changes in treated HepG2 cells. For mechanistic anticancer evaluation, 20 and 40 µg/mL of bacterial extract were examined. The up-regulation of apoptosis-related genes' expression, P53, CASP-3, and BAX/BCL-2 at mRNA and protein levels proved the involvement of P53-dependant mitochondrial apoptotic pathway. The anti-proliferative properties were confirmed by significant G/M cell cycle arrest and PCNA down-regulation in the treated cells. Low cytotoxicity was observed in HPBL compared to HepG2 cells. In conclusion, results suggest that the apoptotic and anti-proliferative effects of Halomonas sp. HA1 extract on HepG2 cells can provide it as a candidate for future pharmaceutical industries.
Islam M El-Garawani, Sabha M El-Sabbagh, Nasser H Abbas, Hany S Ahmed, Omaima A Eissa, Doaa M Abo-Atya, Shaden A M Khalifa, Hesham R El-Seedi

2776 related Products with: A newly isolated strain of Halomonas sp. (HA1) exerts anticancer potential via induction of apoptosis and G/M arrest in hepatocellular carcinoma (HepG2) cell line.

100 µg

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#32615963   2020/07/02 To Up

Mitochondrial DNA copy number in cervical exfoliated cells and risk of cervical cancer among HPV-positive women.

Although human papillomavirus (HPV) infection has been regarded as the cause of cervical cancer in over 99% of cases, only a small fraction of HPV-infected women develop this malignancy. Emerging evidence suggests that alterations of mitochondrial DNA copy number (mtCN) may contribute to carcinogenesis. However, the relationship between mtCN and cervical cancer remains undetermined.
Wei Sun, Xueyun Qin, Jing Zhou, Mingjing Xu, Zhangyan Lyu, Xin Li, Kai Zhang, Min Dai, Ni Li, Dong Hang

1090 related Products with: Mitochondrial DNA copy number in cervical exfoliated cells and risk of cervical cancer among HPV-positive women.



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#32492449   2020/05/31 To Up

The antimicrobial peptide tilapia piscidin 3 induces mitochondria-modulated intrinsic apoptosis of osteosarcoma cells.

Osteosarcoma (OS) is the most common solid tumor of the bone that most often affects adolescents. The introduction of chemotherapy for the treatment of OS has largely improved the survival rates of patients with localized tumors. However, the 5-year survival rate of OS patients with relapsed or metastatic disease is only 10 to 20%. In this study, the antimicrobial peptide tilapia piscidin 3 (TP3), isolated from Nile tilapia (Oreochromis niloticus), was treated to OS MG63 cells. Our findings showed that TP3 concentration as low as 1 μM induced significant inhibition of cell viability and increased DNA fragmentation, as determined by the MTT and TUNEL assays, respectively. The protein expression levels of cleaved caspases 3/9 were increased. An in situ live-cell time-lapse video and cell tomographic microscopy images showed cellular blebbing, shrinkage, nuclear fragmentation, and chromatin condensation, with the formation of beaded apoptopodia. Moreover, there were significant increase in the production of TP3-induced mitochondrial and cellular reactive oxygen species (ROS), as well as down-regulated mitochondrial oxygen consumption and extracellular acidification rates. Additionally, TP3 enhanced mitochondrial fission, whereas fusion was attenuated. Furthermore, after administration of the mitochondria targeted antioxidant mitoTempo, TP3-induced ROS oxidant levels and alterations in cleaved caspases 3/9 expression were rescued. TP3 promoted mitochondria-modulated intrinsic apoptosis through the induction of ROS production, activation of caspases 3/9, and the down-regulation of mitochondrial oxygen consumption and extracellular acidification rates, suggesting that TP3 has potential as an innovative alternative for OS treatment.
Chien-Han Yuan, Yi-Ling Ma, Po-Chang Shih, Chao-Ting Chen, Shu-Yu Cheng, Chieh-Yu Pan, Yen-Hsuan Jean, Yih-Min Chu, Sung-Chun Lin, Yu-Cheng Lai, Hsiao-Mei Kuo

2497 related Products with: The antimicrobial peptide tilapia piscidin 3 induces mitochondria-modulated intrinsic apoptosis of osteosarcoma cells.

50 ug30ml1.00 flask50 ug50 ug96 assays50 ug50 ug30ml100ug Lyophilized50 ug30ml

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#32168762   2020/03/11 To Up

Standards for Methods Utilizing Environmental DNA for Detection of Fish Species.

Environmental DNA (eDNA) techniques are gaining attention as cost-effective, non-invasive strategies for acquiring information on fish and other aquatic organisms from water samples. Currently, eDNA approaches are used to detect specific fish species and determine fish community diversity. Various protocols used with eDNA methods for aquatic organism detection have been reported in different eDNA studies, but there are no general recommendations for fish detection. Herein, we reviewed 168 papers to supplement and highlight the key criteria for each step of eDNA technology in fish detection and provide general suggestions for eliminating detection errors. Although there is no unified recommendation for the application of diverse eDNA in detecting fish species, in most cases, 1 or 2 L surface water collection and eDNA capture on 0.7-μm glass fiber filters followed by extraction with a DNeasy Blood and Tissue Kit or PowerWater DNA Isolation Kit are useful for obtaining high-quality eDNA. Subsequently, species-specific quantitative polymerase chain reaction (qPCR) assays based on mitochondrial cytochrome b gene markers or eDNA metabarcoding based on both and markers via high-throughput sequencing can effectively detect target DNA or estimate species richness. Furthermore, detection errors can be minimized by mitigating contamination, negative control, PCR replication, and using multiple genetic markers. Our aim is to provide a useful strategy for fish eDNA technology that can be applied by researchers, advisors, and managers.
Lu Shu, Arne Ludwig, Zuogang Peng

1140 related Products with: Standards for Methods Utilizing Environmental DNA for Detection of Fish Species.

96T1 LITRE0.1 ml100 ml25 µg1 ml 100ul25 µg0.25 mg 100ul0.2 mg

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#31959368   2020/01/07 To Up

An Unexpected Case of Lagochilascariasis: Interdisciplinary Management and Use of 12S and 18S rDNA Analysis.

A Mexican 24-year-old male patient was referred to our hospital due to increased left retroauricular volume with skin fistulisation, resembling an infection by the uncommon worm Lagochilascaris minor. The patient was submitted to lateral skull base surgery. No adult worms or eggs were observed during light and scanning electron microscopy analysis, as well as by histopathologic examination of the small piece of removed tissue, only L3 stage larvae of Lagochilascaris spp. were identified. Polymerase chain reaction-sequencing assays were performed using primers for the mitochondrial 12S and the nuclear 18S rDNA gene. DNA of some L minor adults, previously identified, were used as control. The molecular analysis identified the worm as L minor. According to previous reports, lagochilascariasis is a complicated infection that requires an interdisciplinary management by different clinical specialists. This is the first time that 12S and 18S rDNA genes are reported as molecular markers for diagnosis of L minor.
Fernando Martinez-Hernandez, Hector Manuel Prado-Calleros, Juan Pablo Ramirez-Hinojosa, Vladimir Figueroa-Angel, Maria Teresa Lopez-Reynoso, Maria Del Carmen Jimenez-Andrade, Isaias Estrada-Moscoso, Nancy Rivas, Javier Escobedo-Ortegon, Ana Flisser, Mirza Gabriela Romero-Valdovinos, Pablo Maravilla

1505 related Products with: An Unexpected Case of Lagochilascariasis: Interdisciplinary Management and Use of 12S and 18S rDNA Analysis.

1000 5 G 5 G25 mg1 g100ug96 wells (1 kit)0.1 mg100ug10 mg100 mg200ul

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#31579947   2019/10/03 To Up

The alkylaminoalkanethiosulfuric acids exhibit in-vitro antileishmanial activity against Leishmania (Viannia) braziliensis: a new perspective for use of these schistosomicidal agents.

The alkylaminoalkanethiosulfuric acids (AAATs) are amphipathic compounds effective against experimental schistosomiasis, of low toxicity, elevated bioavailability after a single oral dose and prompt tissue absorption.
Gabriane Nascimento Porcino, Luciana Maria Ribeiro Antinarelli, Ana Carolina Ribeiro Gomes Maia, Priscila Faria-Pinto, Alessandro Taunay-Rodrigues, Paulo Marcos Zech Coelho, David Lee Nelson, Marcus Luiz Oliveira Penido, Elaine Soares Coimbra, Eveline Gomes Vasconcelos

1941 related Products with: The alkylaminoalkanethiosulfuric acids exhibit in-vitro antileishmanial activity against Leishmania (Viannia) braziliensis: a new perspective for use of these schistosomicidal agents.

96 Tests50 assays100 assays 1 G1.0mg0.1 mg 96 Tests

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#31488073   2019/09/05 To Up

Molecular characterization of human isolates of Strongyloides stercoralis and Rhabditis spp. based on mitochondrial cytochrome c oxidase subunit 1 (cox1).

Due to the similarity of Strongyloides stercoralis with free-living nematodes of Rhabditis species they might be miss-diagnosed with each other in microscopical examination of stool samples. The aim of this study was molecular characterization and differentiation of human derived isolates of S. stercoralis and Rhabditis species based on the mitochondrial gene of cytochrome c oxidase subunit 1 (cox1) amplification.
Mandana Fadaei Tehrani, Meysam Sharifdini, Farzaneh Zahabiun, Robabeh Latifi, Eshrat Beigom Kia

2826 related Products with: Molecular characterization of human isolates of Strongyloides stercoralis and Rhabditis spp. based on mitochondrial cytochrome c oxidase subunit 1 (cox1).

100ul 100ul 100ul20 ug 100ul 100 UG100 ul1000 100ul 100ul 100ul 100ul

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#31378166   2019/08/05 To Up

PCR monitoring of parasitemia during drug treatment for canine Chagas disease.

To date, there is no clear standard to monitor drug treatment for canine Chagas disease. We used 2 real-time PCR (rtPCR) assays targeting kinetoplast DNA (kDNA) and nuclear satellite DNA (nDNA) to detect in canine whole blood. Samples were collected randomly from 131 untreated dogs with unknown infection status in Texas. The kDNA-based rtPCR was slightly more sensitive (diagnostic sensitivity of kDNA = 49% vs. nDNA = 44%; = 0.5732) but slightly less specific (diagnostic specificity of kDNA = 96% vs. nDNA = 97%; > 0.9999) than the nDNA-based rtPCR. However, the differences in sensitivity and specificity between the nDNA- and kDNA-based rtPCR assays were not statistically significant. Using the nDNA- and kDNA-based qualitative rtPCR assays to monitor parasitemia from 137 itraconazole- and amiodarone-treated cases with nDNA- and kDNA-based PCR-positive baselines showed that the PCR positive rate decreased to 0% in 30 d. Using kDNA-based quantitative rtPCR to monitor normalized DNA copies in 4 representative dogs demonstrated that drug treatment could reduce parasite loads within 7-30 d. The kDNA-based qualitative rtPCR may be used for routine parasitemia screening of drug-treated Chagas-positive dogs, whereas nDNA-based qualitative rtPCR may be used for confirmation.
Chih-Ling Zao, Ya-Chin Yang, Lisa Tomanek, Anthony Cooke, Ron Berger, Lung-Chang Chien, Roy Madigan

1426 related Products with: PCR monitoring of parasitemia during drug treatment for canine Chagas disease.

0.25 mg10ml96 Tests/kit1 mL 100 G500mg100 assays1000mg

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