Only in Titles

           Search results for: K99    

paperclip

#32212237   // Save this To Up

N-acetylcysteine reduces cocaine-seeking behavior and anterior cingulate glutamate/glutamine levels among cocaine-dependent individuals.

N-acetylcysteine (NAC) is a cystine prodrug shown to reduce cocaine- and cue-primed reinstatement of cocaine-seeking behavior in preclinical studies. In this inpatient study, the effects of NAC maintenance versus placebo on cocaine-seeking behavior were examined during cocaine-primed and unprimed self-administration sessions among non-treatment-seeking, cocaine-dependent individuals. Twelve participants completed this double-blind, placebo-controlled, within-subject crossover study. Each participant was maintained for 1 week (Sat-Fri) on NAC (1200-mg TID; 3600 mg/day total) and 1 week on placebo (0-mg TID); medication order was randomized. A subset of participants underwent proton magnetic resonance spectroscopy scans (n = 8) on the third day of medication (Mon) to assess neurochemistry in the rostral anterior cingulate (rACC; voxel = 4.5 cm ). In four randomized sessions (Tue-Fri) each week, each participant could earn unit amounts of cocaine (10 mg, fixed) versus money ($0.50 vs. $1.50) on a choice, progressive ratio schedule after insufflating active versus placebo cocaine-priming doses (110 mg vs. 4 mg). Relative to the placebo priming dose, the active cocaine priming dose (110 mg) increased cocaine-seeking behavior (p = .003). NAC reduced cocaine-primed cocaine-seeking behavior compared with placebo levels (p = .044) but did not alter placebo-primed cocaine-seeking behavior. The larger money alternative ($1.50) suppressed cocaine-seeking behavior relative to the smaller money alternative ($0.50; p = .011). Compared with placebo levels, NAC significantly decreased rACC glutamate + glutamine levels (p = .035) and numerically decreased rACC glutamate levels (p = .085). These preliminary findings indicate that NAC suppresses cocaine-seeking behavior in some, but not all, experimental scenarios. Further, our findings suggest NAC may exert its therapeutic effects by modulating excitatory tone in the rACC.

2174 related Products with: N-acetylcysteine reduces cocaine-seeking behavior and anterior cingulate glutamate/glutamine levels among cocaine-dependent individuals.

Anti Rat VGLUT 2, Rabbit Anti VGLUT 1 Rat, polyclo Cocaine test strip Cocaine Benzoylecgonine ( Androst-4-ene-3,6,17-trio Rabbit Anti-IEX1 Differen 3-O-Acetyl-17-O-tert-buty EnzyChrom™ Glutamate As CAR,Car,Constitutive andr Malic enzyme 2, NAD(+) de DMEM F12 w o L Glutamine ∆1-Androstene-3α,17β-

Related Pathways

paperclip

#32208170   // Save this To Up

Methyl-Metabolite Depletion Elicits Adaptive Responses to Support Heterochromatin Stability and Epigenetic Persistence.

S-adenosylmethionine (SAM) is the methyl-donor substrate for DNA and histone methyltransferases that regulate epigenetic states and subsequent gene expression. This metabolism-epigenome link sensitizes chromatin methylation to altered SAM abundance, yet the mechanisms that allow organisms to adapt and protect epigenetic information during life-experienced fluctuations in SAM availability are unknown. We identified a robust response to SAM depletion that is highlighted by preferential cytoplasmic and nuclear mono-methylation of H3 Lys 9 (H3K9) at the expense of broad losses in histone di- and tri-methylation. Under SAM-depleted conditions, H3K9 mono-methylation preserves heterochromatin stability and supports global epigenetic persistence upon metabolic recovery. This unique chromatin response was robust across the mouse lifespan and correlated with improved metabolic health, supporting a significant role for epigenetic adaptation to SAM depletion in vivo. Together, these studies provide evidence for an adaptive response that enables epigenetic persistence to metabolic stress.

2209 related Products with: Methyl-Metabolite Depletion Elicits Adaptive Responses to Support Heterochromatin Stability and Epigenetic Persistence.

6-Acetoxymethyl-4-methoxy N Methyl N nitroso p tolu 3-O-Benzyl-4-O-methyl Tol N Methyl N nitroso p tolu 4-Amino-N-methyl-α-tolue N alpha 4 Tosyl L arginin Total Oxidant Status (TOS (1R,2S,3R,5S)-5-Acetyloxy N (tert Butyldimethylsily Homogenizer for 24 sample 1-Amino-1-cyclopentanecar 3,5 Dimethoxybenzoic acid

Related Pathways

paperclip

#32206720   // Save this To Up

Alcohol dependence potentiates substance P/neurokinin-1 receptor signaling in the rat central nucleus of amygdala.

Behavioral and clinical studies suggest a critical role of substance P (SP)/neurokinin-1 receptor (NK-1R) signaling in alcohol dependence. Here, we examined regulation of GABA transmission in the medial subdivision of the central amygdala (CeM) by the SP/NK-1R system, and its neuroadaptation following chronic alcohol exposure. In naïve rats, SP increased action potential-dependent GABA release, and the selective NK-1R antagonist L822429 decreased it, demonstrating SP regulation of CeM activity under basal conditions. SP induced a larger GABA release in alcohol-dependent rats accompanied by decreased NK-1R expression compared to naïve controls, suggesting NK-1R hypersensitivity which persisted during protracted alcohol withdrawal. The NK-1R antagonist blocked acute alcohol-induced GABA release in alcohol-dependent and withdrawn but not in naïve rats, indicating that dependence engages the SP/NK-1R system to mediate acute effects of alcohol. Collectively, we report long-lasting CeA NK-1R hypersensitivity corroborating that NK-1Rs are promising targets for the treatment of alcohol use disorder.

2686 related Products with: Alcohol dependence potentiates substance P/neurokinin-1 receptor signaling in the rat central nucleus of amygdala.

Rabbit Anti-Insulin Recep IGF-1R Signaling Phospho- Rabbit Anti-Insulin Recep Rabbit Anti-Insulin Recep Rabbit Anti-Insulin Recep Rabbit Anti-Insulin Recep Rabbit Anti-Insulin Recep Rabbit Anti-Insulin Recep Rabbit Anti-Insulin Recep Rabbit Anti-Insulin Recep Rabbit Anti-Insulin Recep Rabbit Anti-Insulin Recep

Related Pathways

paperclip

#32197946   // Save this To Up

Sex differences in redox homeostasis in renal disease.

Sex differences in redox signaling in the kidney present new challenges and opportunities for understanding the physiology and pathophysiology of the kidney. This review will focus on reactive oxygen species, immune-related signaling pathways and endothelin-1 as potential mediators of sex-differences in redox homeostasis in the kidney. Additionally, this review will highlight male-female differences in redox signaling in several major cardiovascular and renal disorders namely acute kidney injury, diabetic nephropathy, kidney stone disease and salt-sensitive hypertension. Furthermore, we will discuss the contribution of redox signaling in the pathogenesis of postmenopausal hypertension and preeclampsia.

1982 related Products with: Sex differences in redox homeostasis in renal disease.

Renal disease spectrum ti rHIV gp36, insoluble Anti Kidney disease spectrum ( Colon disease spectrum (c Small intestine disease s Anti 3 DG imidazolone Mon Lung disease spectrum tis Cervical intraepithelial Ovarian disease spectrum Beta Amyloid (1 40) ELISA rHIV gp41, soluble Antige Breast disease spectrum t

Related Pathways

paperclip

#32194980   // Save this To Up

Network-based drug repurposing for novel coronavirus 2019-nCoV/SARS-CoV-2.

Human coronaviruses (HCoVs), including severe acute respiratory syndrome coronavirus (SARS-CoV) and 2019 novel coronavirus (2019-nCoV, also known as SARS-CoV-2), lead global epidemics with high morbidity and mortality. However, there are currently no effective drugs targeting 2019-nCoV/SARS-CoV-2. Drug repurposing, representing as an effective drug discovery strategy from existing drugs, could shorten the time and reduce the cost compared to de novo drug discovery. In this study, we present an integrative, antiviral drug repurposing methodology implementing a systems pharmacology-based network medicine platform, quantifying the interplay between the HCoV-host interactome and drug targets in the human protein-protein interaction network. Phylogenetic analyses of 15 HCoV whole genomes reveal that 2019-nCoV/SARS-CoV-2 shares the highest nucleotide sequence identity with SARS-CoV (79.7%). Specifically, the envelope and nucleocapsid proteins of 2019-nCoV/SARS-CoV-2 are two evolutionarily conserved regions, having the sequence identities of 96% and 89.6%, respectively, compared to SARS-CoV. Using network proximity analyses of drug targets and HCoV-host interactions in the human interactome, we prioritize 16 potential anti-HCoV repurposable drugs (e.g., melatonin, mercaptopurine, and sirolimus) that are further validated by enrichment analyses of drug-gene signatures and HCoV-induced transcriptomics data in human cell lines. We further identify three potential drug combinations (e.g., sirolimus plus dactinomycin, mercaptopurine plus melatonin, and toremifene plus emodin) captured by the "" pattern: the targets of the drugs both hit the HCoV-host subnetwork, but target separate neighborhoods in the human interactome network. In summary, this study offers powerful network-based methodologies for rapid identification of candidate repurposable drugs and potential drug combinations targeting 2019-nCoV/SARS-CoV-2.

1556 related Products with: Network-based drug repurposing for novel coronavirus 2019-nCoV/SARS-CoV-2.

MarkerGene™ Multiple Dr Recombinant Viral antige SARS Coronavirus IgG ELIS Recombinant Viral antige Mouse Anti-SARS-Associate SARS Associated Coronavir Mouse Anti-SARS-Associate Recombinant Viral antige Rabbit Anti-SARS-Associat MarkerGeneTM Fluorescent SARS Associated Coronavir Mouse Anti-SARS-Associate

Related Pathways

paperclip

#32190716   // Save this To Up

E-cigarette Product Preferences among Adult Smokers: A Discrete Choice Experiment.

In this study, we used a discrete choice experiment (DCE) conducted August-October 2017 to examine electronic nicotine delivery systems (ENDS) product preferences in a national sample of adult smokers (N = 1154) who were also using ENDS or had not ruled out future use.

2276 related Products with: E-cigarette Product Preferences among Adult Smokers: A Discrete Choice Experiment.

HyAgarose™ HR Agarose, Biocidal ZF, spray disinf MOUSE ANTI BOVINE ROTAVIR Custom Polyclonal Antibod Anti CML Monoclonal Antib Anti 3 DG imidazolone Mon RABBIT ANTI GSK3 BETA (pS HyAgarose™ HR Agarose, MOUSE ANTI HUMAN CD15, Pr ReadiLink™ mFluor™ Vi Expression Media Products Rat monoclonal anti mouse

Related Pathways

paperclip

#32181348   // Save this To Up

A molecular rack and pinion actuates a cell-surface adhesin and enables bacterial gliding motility.

The gliding bacterium is known to have an adhesin, SprB, that moves along the cell surface on a spiral track. Following viscous shear, cells can be tethered by the addition of an anti-SprB antibody, causing spinning at 3 Hz. Labeling the type 9 secretion system (T9SS) with a YFP fusion of GldL showed a yellow fluorescent spot near the rotation axis, indicating that the motor driving the motion is associated with the T9SS. The distance between the rotation axis and the track (90 nm) was determined after adding a Cy3 label for SprB. A rotary motor spinning a pinion of radius 90 nm at 3 Hz would cause a spot on its periphery to move at 1.5 μm/s, the gliding speed. We suggest the pinion drives a flexible tread that carries SprB along a track fixed to the cell surface. Cells glide when this adhesin adheres to the solid substratum.

2347 related Products with: A molecular rack and pinion actuates a cell-surface adhesin and enables bacterial gliding motility.

∆2-Androstene-1α,17β- Androgen Receptor (Phosph Androstenedione 19 Goat Anti-Human Androgen Androgen Receptor , Mouse Andrographolide C20H30O5 Andarine C19H18F3N3O6� rac Androst-16-en-2,2,5,6 3-O-Acetyl 5,14-Androstad CAR,CAR,Constitutive acti Androstane-3a,17b-diol Gl 4 Androstene 3,17 dione C

Related Pathways

paperclip

#32166602   // Save this To Up

Asymmetry and Microstructure of Temporal-Suppression Patterns in Basilar-Membrane Responses to Clicks: Relation to Tonal Suppression and Traveling-Wave Dispersion.

The cochlea's wave-based signal processing allows it to efficiently decompose a complex acoustic waveform into frequency components. Because cochlear responses are nonlinear, the waves arising from one frequency component of a complex sound can be altered by the presence of others that overlap with it in time and space (e.g., two-tone suppression). Here, we investigate the suppression of basilar-membrane (BM) velocity responses to a transient signal (a test click) by another click or tone. We show that the BM response to the click can be reduced when the stimulus is shortly preceded or followed by another (suppressor) click. More surprisingly, the data reveal two curious dependencies on the interclick interval, Δt. First, the temporal suppression curve (amount of suppression vs. Δt) manifests a pronounced and nearly periodic microstructure. Second, temporal suppression is generally strongest not when the two clicks are presented simultaneously (Δt = 0), but when the suppressor click precedes the test click by a time interval corresponding to one to two periods of the best frequency (BF) at the measurement location. By systematically varying the phase of the suppressor click, we demonstrate that the suppression microstructure arises from alternating constructive and destructive interference between the BM responses to the two clicks. And by comparing temporal and tonal suppression in the same animals, we test the hypothesis that the asymmetry of the temporal-suppression curve around Δt = 0 stems from cochlear dispersion and the well-known asymmetry of tonal suppression around the BF. Just as for two-tone suppression, BM responses to clicks are most suppressed by tones at frequencies just above the BF of the measurement location. On average, the frequency place of maximal suppressibility of the click response predicted from temporal-suppression data agrees with the frequency at which tonal suppression peaks, consistent with our hypothesis.

1904 related Products with: Asymmetry and Microstructure of Temporal-Suppression Patterns in Basilar-Membrane Responses to Clicks: Relation to Tonal Suppression and Traveling-Wave Dispersion.

Homogenizer for 24 sample FDA Standard Frozen Tissu Pig translocase of outer Nycodenz, non ionic, non Native Influenza HA (A To Rabbit Anti-Human Toll In Cell Meter™ Fluorimetri ELISA Mouse , Interleukin FDA Standard Frozen Tissu (BCIP Toluidine)5 Bromo 4 FIV Core Ag, recombinant Native Influenza HA (A To

Related Pathways