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#32791294   2020/08/10 To Up

The cold-soaking extract of Chinese yam (Dioscorea opposita Thunb.) protects against erectile dysfunction by ameliorating testicular function in hydrocortisone-induced KDS-Yang rats and in oxidatively damaged TM3 cells.

Clinical applications and pharmacological research suggest that Dioscorea opposita Thunb. (Chinese yam), a well-known traditional Chinese medicine which has been used for more than 2000 years to nourish kidney-yang and protect the male reproductive system, might be efficacious for the treatment of erectile dysfunction (ED).
Shiting Yu, Bing Han, Xueyuan Bai, Shichao Liu, Xin Xing, Daqing Zhao, Meichen Liu, Siming Wang

2155 related Products with: The cold-soaking extract of Chinese yam (Dioscorea opposita Thunb.) protects against erectile dysfunction by ameliorating testicular function in hydrocortisone-induced KDS-Yang rats and in oxidatively damaged TM3 cells.

400 ug1 mg1 mg10 rxns400 ug-1.00 flask1x10e7 cells96 assays100ug50 ul100 µg

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#32791199   2020/08/10 To Up

Protocol for a national probability survey using home specimen collection methods to assess prevalence and incidence of SARS-CoV-2 infection and antibody response.

The US response to the SARS-CoV-2 epidemic has been hampered by early and ongoing delays in testing for infection; without data on where infections were occurring and the magnitude of the epidemic, early public health responses were not data-driven. Understanding the prevalence of SARS-CoV-2 infections and immune response is critical to developing and implementing an effective public health response. Most serological surveys have been limited to localities that opted to conduct them and/or were based on convenience samples. Moreover, results of antibody testing are subject to high false positive rates due to a presumably low prevalence of seroconversion and imperfect test specificity.
Aaron J Siegler, Patrick S Sullivan, Travis Sanchez, Ben Lopman, Mansour Fahimi, Charles Sailey, Martin Frankel, Richard Rothenberg, Colleen F Kelley, Heather Bradley

1895 related Products with: Protocol for a national probability survey using home specimen collection methods to assess prevalence and incidence of SARS-CoV-2 infection and antibody response.

200ul200ug100ug50 ug 25UG200ug1,000 tests100ul100ug100ul10 mg

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#32790727   2020/08/13 To Up

Excreta biomarkers in response to different gut barrier dysfunction models and probiotic supplementation in broiler chickens.

Increased intestinal permeability (IP) and inflammation are both linked with functionality of the intestinal barrier and in particular enterocytes. Currently, almost all assessment methods of the intestinal barrier function are invasive. The present study aimed to quantify selected proteins as novel biomarkers in excreta of broiler chickens to facilitate non-invasive assessment of gut barrier function using enzyme-linked immunosorbent assays (ELISA). It was further hypothesised that probiotics as feed additives may counteract gut barrier dysfunction. A 3 × 2 factorial arrangement of treatments was used with the main factors being gut barrier dysfunction models (control, rye-based diet, and dexamethasone-DEX) with and without probiotic supplementation (a three-strain Bacillus) using 72 male Ross 308 day-old chickens. Each of the 6 experimental treatments was replicated 12 times. On d 21 of age, fluorescein isothiocyanate dextran (FITC-d) uptake into serum was examined to test IP. Fresh excreta samples were collected on d 20. The biomarkers included alpha-1 antitrypsin (A1AT), intestinal fatty acid binding protein (I-FABP), lipocalin-2 (LCN2), fibronectin (FN), intestinal alkaline phosphatase (IAP), ovotransferrin (OVT) and superoxide dismutase [Cu-Zn] (SOD1). Only DEX increased (P<0.001) FITC-d passage to the blood on d 21 of age, indicating a greater IP. The excreta concentrations of A1AT, I-FABP and SOD1 were unaltered by the experimental treatments. DEX increased (P<0.05) FN concentration in excreta compared with control birds. Conversely, inclusion of rye in the diet reduced (P<0.05) FN but increased (P<0.001) OVT in excreta. Independently, DEX decreased IAP (P<0.05) in excreta compared with control and rye-fed birds. The excreta concentration of LCN2 tended (P = 0.086) to increase in birds injected by DEX. There was no demonstrable effect of probiotic addition on any of the studied parameters. Among the tested biomarkers, FN, IAP, and LCN2 revealed promise as biomarkers of intestinal barrier function quantified by ELISA kits.
Reza Barekatain, Gordon S Howarth, Nicky-Lee Willson, David Cadogan, Stuart Wilkinson

1111 related Products with: Excreta biomarkers in response to different gut barrier dysfunction models and probiotic supplementation in broiler chickens.

1 kit500 gm. 1 G1mg100 μg100 1 kit100 mg5025

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#32789118   2020/07/27 To Up

IgE recognition profile of aeroallergen components in young children sensitized to dogs.

Pet ownership is increasing rapidly and as growing numbers of dogs in household, clinicians are facing more allergic patients and so as in young children.
Jeongmin Lee, Kyunguk Jeong, Se-Ah Jeon, Sooyoung Lee

2341 related Products with: IgE recognition profile of aeroallergen components in young children sensitized to dogs.

100 μg50 ul501 mg1 kit100 mg96 wells10100 100 ul

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#32788913   2020/07/20 To Up

Current coronavirus (SARS-CoV-2) epidemiological, diagnostic and therapeutic approaches: An updated review until June 2020.

Coronaviruses are a group of enveloped viruses with non-segmented, single-stranded, and positive-sense RNA genomes. In December 2019, an outbreak of coronavirus disease 2019 (COVID-19) caused by the novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), in Wuhan City, China. The World Health Organization (WHO) declared the coronavirus outbreak as a global pandemic in March 2020. Fever, dry cough and fatigue are found in the vast majority of all COVID-19 cases. Early diagnosis, treatment and future prevention are keys to COVID-19 management. Currently, the unmet need to develop cost-effective point-of-contact test kits and efficient laboratory techniques for confirmation of COVID-19 infection has powered a new frontier of diagnostic innovation. No proven effective therapies or vaccines for SARS-CoV-2 currently exist. The rapidly increasing research regarding COVID-19 virology provides a significant number of potential drug targets. Remdesivir may be the most promising therapy up till now. On May 1, 2020, Gilead Sciences, announced that the U.S. Food and Drug Administration (FDA) has granted emergency use authorization (EUA) for the investigational Remdesivir as a potential antiviral for COVID-19 treatment. On May 7, 2020, Gilead Sciences, announced that the Japanese Ministry of Health, Labour and Welfare (MHLW) has granted regulatory approval of Veklury® (Remdesivir) as a treatment for SARS-CoV-2 infection, the virus that causes COVID-19 acute respiratory syndrome, under an exceptional approval pathway. Also, Corticosteroids are recommended for severe cases only to suppress the immune response and reduce symptoms, but not for mild and moderate patients where they are associated with a high-risk side effect. Based on the currently published evidence, we tried to highlight different diagnostic approaches, side effects and therapeutic agents that could help physicians in the frontlines.
Ahmed Nabil, Koichiro Uto, Mohamed M Elshemy, Reham Soliman, Ayman A Hassan, Mitsuhiro Ebara, Gamal Shiha

2629 related Products with: Current coronavirus (SARS-CoV-2) epidemiological, diagnostic and therapeutic approaches: An updated review until June 2020.

200 100 ug/vial100 µg100 100 1mg100 µg200

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#32787398   2020/08/05 To Up

Coronavirus-19 and malaria: The great mimics.

The use of SARS-CoV-2 rapid diagnostic test (RDT) kits by some African countries for screening has raised serious concerns over their role in malaria areas. Coupled with a lack of adequate personal protective equipment and the scarcity of knowledge on the possible interaction between malaria and COVID-19 both in terms of presentations and shared symptoms, this has left many frontline health workers with fears and anxieties. Several anecdotal reports have already raised questions pertaining to possible false-positive COVID-19 results in proven malaria cases by use of SARS-CoV-2 RDT kits with huge costs to already constrained budgets. The report raises concerns on the use of SARS-CoV-2 kits in malaria areas in terms of cost, to prompt research, allay fears and guide policy during this pandemic and beyond.
Tapiwanashe Kusotera, Trust G Nhengu

2726 related Products with: Coronavirus-19 and malaria: The great mimics.

100 1 5 G10 mg1 g100 ul5mg500 Units5 g100ug Lyophilized500 MG25 mg

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#32785665   2020/08/12 To Up

Usability and acceptability of home-based self-testing for SARS-CoV-2 antibodies for population surveillance.

This study assesses acceptability and usability of home-based self-testing for SARS-CoV-2 antibodies using lateral flow immunoassays (LFIA).
Christina Atchison, Philippa Pristerà, Emily Cooper, Vasiliki Papageorgiou, Rozlyn Redd, Maria Piggin, Barnaby Flower, Gianluca Fontana, Sutha Satkunarajah, Hutan Ashrafian, Anna Lawrence-Jones, Lenny Naar, Jennifer Chigwende, Steve Gibbard, Steven Riley, Ara Darzi, Paul Elliott, Deborah Ashby, Wendy Barclay, Graham S Cooke, Helen Ward

2591 related Products with: Usability and acceptability of home-based self-testing for SARS-CoV-2 antibodies for population surveillance.

2x5L250 mg 2x5L 1 G2x96 well plate2000 rxn100 10 mg 1000 ml 1 mg0.2 mg

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#32784757   2020/08/07 To Up

SARS-CoV-2 RNA Extraction Using Magnetic Beads for Rapid Large-Scale Testing by RT-qPCR and RT-LAMP.

Rapid large-scale testing is essential for controlling the ongoing pandemic of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The standard diagnostic pipeline for testing SARS-CoV-2 presence in patients with an ongoing infection is predominantly based on pharyngeal swabs, from which the viral RNA is extracted using commercial kits, followed by reverse transcription and quantitative PCR detection. As a result of the large demand for testing, commercial RNA extraction kits may be limited and, alternatively, non-commercial protocols are needed. Here, we provide a magnetic bead RNA extraction protocol that is predominantly based on in-house made reagents and is performed in 96-well plates supporting large-scale testing. Magnetic bead RNA extraction was benchmarked against the commercial QIAcube extraction platform. Comparable viral RNA detection sensitivity and specificity were obtained by fluorescent and colorimetric reverse transcription loop-mediated isothermal amplification (RT-LAMP) using a primer set targeting the N gene, as well as RT-qPCR using a primer set targeting the E gene, showing that the RNA extraction protocol presented here can be combined with a variety of detection methods at high throughput. Importantly, the presented diagnostic workflow can be quickly set up in a laboratory without access to an automated pipetting robot.
Steffen Klein, Thorsten G Müller, Dina Khalid, Vera Sonntag-Buck, Anke-Mareil Heuser, Bärbel Glass, Matthias Meurer, Ivonne Morales, Angelika Schillak, Andrew Freistaedter, Ina Ambiel, Sophie L Winter, Liv Zimmermann, Tamara Naumoska, Felix Bubeck, Daniel Kirrmaier, Stephanie Ullrich, Isabel Barreto Miranda, Simon Anders, Dirk Grimm, Paul Schnitzler, Michael Knop, Hans-Georg Kräusslich, Viet Loan Dao Thi, Kathleen Börner, Petr Chlanda

1901 related Products with: SARS-CoV-2 RNA Extraction Using Magnetic Beads for Rapid Large-Scale Testing by RT-qPCR and RT-LAMP.

2x5L1 mL at 10 mg/mLLabels 2 mg of Ab4X1.25ml; 200 reactions (25ml11 mL at 10 mg/mL25ml 25ml200ul1 ml25ml

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