Search results for: Native Human Amylase alpha Proteins
#38851735 
2024/06/08
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Biological, phytochemical and molecular docking characteristics of Laurus nobilis L. fresh leaves essential oil from Palestine.
The historical use of Laurus nobilis L., the plant is native to the Mediterranean region and has been cultivated for its aromatic leaves, which are used as a flavoring agent in cooking and for their potential therapeutic properties.Nidal Jaradat, Mohammed Hawash, Mohammed T Qaoud, Nawaf Al-Maharik, Mohammad Qadi, Fatimah Hussein, Linda Issa, Ahmad Saleh, Laith Saleh, Ahmad Jadallah
1620 related Products with: Biological, phytochemical and molecular docking characteristics of Laurus nobilis L. fresh leaves essential oil from Palestine.
50 ug10 ug10 mg100ug 6 ml Ready-to-use 10 mg1000 100 mg1 ml1,000 tests200ug
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#38177679 2024/01/04
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The Ruminococcus bromii amylosome protein Sas6 binds single and double helical α-glucan structures in starch.
Resistant starch is a prebiotic accessed by gut bacteria with specialized amylases and starch-binding proteins. The human gut symbiont Ruminococcus bromii expresses Sas6 (Starch Adherence System member 6), which consists of two starch-specific carbohydrate-binding modules from family 26 (RbCBM26) and family 74 (RbCBM74). Here, we present the crystal structures of Sas6 and of RbCBM74 bound with a double helical dimer of maltodecaose. The RbCBM74 starch-binding groove complements the double helical α-glucan geometry of amylopectin, suggesting that this module selects this feature in starch granules. Isothermal titration calorimetry and native mass spectrometry demonstrate that RbCBM74 recognizes longer single and double helical α-glucans, while RbCBM26 binds short maltooligosaccharides. Bioinformatic analysis supports the conservation of the amylopectin-targeting platform in CBM74s from resistant-starch degrading bacteria. Our results suggest that RbCBM74 and RbCBM26 within Sas6 recognize discrete aspects of the starch granule, providing molecular insight into how this structure is accommodated by gut bacteria.Amanda L Photenhauer, Rosendo C Villafuerte-Vega, Filipe M Cerqueira, Krista M Armbruster, Filip Mareček, Tiantian Chen, Zdzislaw Wawrzak, Jesse B Hopkins, Craig W Vander Kooi, Štefan Janeček, Brandon T Ruotolo, Nicole M Koropatkin
1214 related Products with: The Ruminococcus bromii amylosome protein Sas6 binds single and double helical α-glucan structures in starch.
1 Set1 Set1 Set1 Set100ug Lyophilized100ug Lyophilized1 Set1 Set1 Set1 Set100ug Lyophilized1 Set
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#38009757 2023/11/27
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Papain-Based Solubilization of Decellularized Extracellular Matrix for the Preparation of Bioactive, Thermosensitive Pregels.
Solubilized, gel-forming decellularized extracellular matrix (dECM) is used in a wide range of basic and translational research and due to its inherent bioactivity can promote structural and functional tissue remodeling. The animal-derived protease pepsin has become the standard proteolytic enzyme for the solubilization of almost all types of collagen-based dECM. In this study, pepsin was compared with papain, α-amylase, and collagenase for their potential to solubilize porcine liver dECM. Maximum preservation of bioactive components and native dECM properties was used as a decisive criterion for further application of the enzymes, with emphasis on minimal destruction of the protein structure and maintained capacity for physical thermogelation at neutral pH. The solubilized dECM digests, and/or their physically gelled hydrogels were characterized for their rheological properties, gelation kinetics, GAG content, proteomic composition, and growth factor profile. This study highlights papain as a plant-derived enzyme that can serve as a cost-effective alternative to animal-derived pepsin for the efficient solubilization of dECM. The resulting homogeneous papain-digested dECM preserved its thermally triggered gelation properties similar to pepsin digests, and the corresponding dECM hydrogels demonstrated their enhanced bioadhesiveness in single-cell force spectroscopy experiments with fibroblasts. The viability and proliferation of human HepaRG cells on dECM gels were similar to those on pure rat tail collagen type I gels. Papain is not only highly effective and economically attractive for dECM solubilization but also particularly interesting when digesting human-tissue-derived dECM for regenerative applications, where animal-derived materials are to be avoided.Ahed Almalla, Laura Elomaa, Leïla Bechtella, Assal Daneshgar, Prabhu Yavvari, Zeinab Mahfouz, Peter Tang, Beate Koksch, Igor Sauer, Kevin Pagel, Karl Herbert Hillebrandt, Marie Weinhart
1807 related Products with: Papain-Based Solubilization of Decellularized Extracellular Matrix for the Preparation of Bioactive, Thermosensitive Pregels.
5 G100preparations100.00 ul 1 G1 ml25 μg100μg100 50μl1 kit
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#36704898 2023/01/27
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Effects of rice protein, soy isolate protein, and whey concentrate protein on the digestibility and physicochemical properties of extruded rice starch.
Protein, as the second major component in starchy foods, is crucial for its influence on the physicochemical properties and digestibility of starch. However, the effect of different sources of protein on starch digestibility is still unclear. In this paper, the effects of different sources of proteins (rice protein: RP, soybean isolate protein: SPI, and whey concentrate protein: WPC) on structural features, digestibility, and enzyme activity of extruded rice starch were investigated. The addition of all three proteins reduced the starch digestibility of extrudates. Native SPI and WPC suppressed amyloglucosidase activity, and all three proteins exhibited stronger amyloglucosidase inhibition when hydrolyzed. The rheological properties and Fourier transform infrared spectroscopy results revealed the exogenous proteins and starch interacted through non-covalent bonds and improved the ordered structures in the extrudates. The extrusion process also facilitated the formation of a V-type structure. The sum of SDS and RS content of extrudates was negatively correlated with the content of leached amylose and positively correlated with the ratio of 1047/1022 cm . These findings suggest that the inclusion of exogenous proteins during extrusion can affect starch digestibility through mechanisms such as the interaction with starch molecules, as well as the inhibition of amylase activity. PRACTICAL APPLICATION: This result indicated that the addition of protein during extrusion not only increased the nutritional value of the extrudate, but also decreased the starch digestibility. Extrusion technology can efficiently produce extruded products with protein, expanding further applications of protein in food and providing new healthy staple food options for special populations, such as diabetic and overweight people.Huiyi Bao, Qing Liu, Yueyue Yang, Lulian Xu, Kunfu Zhu, Zhengyu Jin, Aiquan Jiao
2317 related Products with: Effects of rice protein, soy isolate protein, and whey concentrate protein on the digestibility and physicochemical properties of extruded rice starch.
1000 TESTS/0.65ml200ml100 U100 ug1 Set100ug1000mg100ug1 mg100ug Lyophilized0.1 mg100ml
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#36037121 2022/08/01
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In Vitro Study on Effect of Zinc Oxide Nanoparticles on the Biological Activities of Croton tiglium L. Seeds Extracts.
Croton tiglium L. seeds were studied against colon cancer induced chemically in rats after incorporating silver nanoparticles (Ag-NPs) but the body has no the ability to discrete silver or silver ions. Therefore, the present study was designed to reveal the biological activities of different C. tiglium L. seeds extracts incorporated with zinc oxide nanoparticles (ZnO-NPs).Wael Mahmoud Aboulthana, Nagwa Ibrahim Omar, Amal Mohamed El-Feky, Enas Ahmed Hasan, Noha El-Sayed Ibrahim, Ahmed Mahmoud Youssef
1403 related Products with: In Vitro Study on Effect of Zinc Oxide Nanoparticles on the Biological Activities of Croton tiglium L. Seeds Extracts.
100ug 25 ml Ready-to-use 2 6 ml 25 MG 2 ml Ready-to-use 1 mg 6 ml Ready-to-use 0.2 mg
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#35889399 2022/07/15
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Molecular Docking and Dynamics Simulation of Natural Compounds from Betel Leaves ( L.) for Investigating the Potential Inhibition of Alpha-Amylase and Alpha-Glucosidase of Type 2 Diabetes.
L. is widely distributed and commonly used medicinally important herb. It can also be used as a medication for type 2 diabetes patients. In this study, compounds of were screened to investigate the inhibitory action of alpha-amylase and alpha-glucosidase against type 2 diabetes through molecular docking, molecular dynamics simulation, and ADMET (absorption, distribution, metabolism, excretion, and toxicity) analysis. The molecule apigenin-7--glucoside showed the highest binding affinity among 123 (one hundred twenty-three) tested compounds. This compound simultaneously bound with the two-target proteins alpha-amylase and alpha-glucosidase, with high molecular mechanics-generalized born surface area (MM/GBSA) values (ΔG Bind = -45.02 kcal mol for alpha-amylase and -38.288 for alpha-glucosidase) compared with control inhibitor acarbose, which had binding affinities of -36.796 kcal mol for alpha-amylase and -29.622 kcal mol for alpha-glucosidase. The apigenin-7--glucoside was revealed to be the most stable molecule with the highest binding free energy through molecular dynamics simulation, indicating that it could compete with the inhibitors' native ligand. Based on ADMET analysis, this phytochemical exhibited a wide range of physicochemical, pharmacokinetic, and drug-like qualities and had no significant side effects, making them prospective drug candidates for type 2 diabetes. Additional in vitro, in vivo, and clinical investigations are needed to determine the precise efficacy of drugs.Sabbir Ahmed, Md Chayan Ali, Rumana Akter Ruma, Shafi Mahmud, Gobindo Kumar Paul, Md Abu Saleh, Mohammed Merae Alshahrani, Ahmad J Obaidullah, Sudhangshu Kumar Biswas, Md Mafizur Rahman, Md Mizanur Rahman, Md Rezuanul Islam
1204 related Products with: Molecular Docking and Dynamics Simulation of Natural Compounds from Betel Leaves ( L.) for Investigating the Potential Inhibition of Alpha-Amylase and Alpha-Glucosidase of Type 2 Diabetes.
5 G 5 G100ug Lyophilized100ug5 g250ul200ug396T1 g100ug Lyophilized3
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#35878751 2022/07/22
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DeepBindBC: A practical deep learning method for identifying native-like protein-ligand complexes in virtual screening.
Identifying native-like protein-ligand complexes (PLCs) from an abundance of docking decoys is critical for large-scale virtual drug screening in early-stage drug discovery lead searching efforts. Providing reliable prediction is still a challenge for most current affinity predicting models because of a lack of non-binding data during model training, lost critical physical-chemical features, and difficulties in learning abstract information with limited neural layers. In this work, we proposed a deep learning model, DeepBindBC, for classifying putative ligands as binding or non-binding. Our model incorporates information on non-binding interactions, making it more suitable for real applications. ResNet model architecture and more detailed atom type representation guarantee implicit features can be learned more accurately. Here, we show that DeepBindBC outperforms Autodock Vina, Pafnucy, and DLSCORE for three DUD.E testing sets. Moreover, DeepBindBC identified a novel human pancreatic α-amylase binder validated by a fluorescence spectral experiment (K = 1.0 × 10 M). Furthermore, DeepBindBC can be used as a core component of a hybrid virtual screening pipeline that incorporating many other complementary methods, such as DFCNN, Autodock Vina docking, and pocket molecular dynamics simulation. Additionally, an online web server based on the model is available at http://cbblab.siat.ac.cn/DeepBindBC/index.php for the user's convenience. Our model and the web server provide alternative tools in the early steps of drug discovery by providing accurate identification of native-like PLCs.Haiping Zhang, Tingting Zhang, Konda Mani Saravanan, Linbu Liao, Hao Wu, Haishan Zhang, Huiling Zhang, Yi Pan, Xuli Wu, Yanjie Wei
1965 related Products with: DeepBindBC: A practical deep learning method for identifying native-like protein-ligand complexes in virtual screening.
400Tests0.1 mg0.1 mg1 Set100 assays100ug Lyophilized1 Set1 Set1 Set1 Set1 Set
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#33537688 2021/02/04
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Modulation of gelatinized wheat starch digestion and fermentation profiles by young apple polyphenols in vitro.
To evaluate the effect of young apple polyphenols (YAP) on starch digestion and gut microbiota, complexes of native wheat starch (NWS) with YAP, and their main components chlorogenic acid (CA) and phlorizin (P) were fabricated and gelatinized. Through XRD and FTIR analysis, it was found that the partial crystalline structure of NWS was destroyed during gelatinization, and the addition of P decreased the extent of destruction. Then, the gelatinized starchy samples were subjected to in vitro digestion. The wheat starch (WS)-phenolic compound complexes significantly suppressed the digestion rate and increased the proportion of resistant starch (RS) in WS. Furthermore, the residual starchy components after digestion were fermented by human fecal samples for 24 h. The WS-YAP complex greatly increased the concentration of short-chain fatty acids (SCFAs), especially acetic and propionic acids, and enhanced the growth of health-promoting gut microbiota such as Prevotella. Conclusively, YAP was shown to play a positive role in maintaining blood glucose balance and intestinal health.Dan Li, Yongli Yang, Xi Yang, Xiaoyu Wang, Chuo Guo, Lijun Sun, Yurong Guo
2823 related Products with: Modulation of gelatinized wheat starch digestion and fermentation profiles by young apple polyphenols in vitro.
96 tests25mg100ug100ug Lyophilized5 g100ug 100 G1 Set16 Arrays/Slide
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#33515684 2021/01/27
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Polyphenol-rich extract and fractions of Terminalia phaeocarpa Eichler possess hypoglycemic effect, reduce the release of cytokines, and inhibit lipase, α-glucosidase, and α-amilase enzymes.
species of Terminalia (Combretaceae) are used to treat diabetes and metabolic disorders in Asia, Africa, and America. Terminalia phaeocarpa Eichler is an endemic tree from Brazil, popularly known as capitão. This species is closely related to Terminalia argentea Mart., also vulgarly known as capitão, a native but not endemic tree. Due to their phenotype similarity, these species might eventually prove inseparable and they are indistinctly used by locals to treat diabetes, among other diseases. The potential antidiabetic effect of T. argentea has been previously reported, whereas the biological effects and chemical composition of T. phaeocarpa have never been addressed so far.José Hugo de Sousa Gomes, Ulrich Carlos Mbiakop, Renata Leite Oliveira, João Renato Stehmann, Rodrigo Maia de Pádua, Steyner F Cortes, Fernão Castro Braga
1514 related Products with: Polyphenol-rich extract and fractions of Terminalia phaeocarpa Eichler possess hypoglycemic effect, reduce the release of cytokines, and inhibit lipase, α-glucosidase, and α-amilase enzymes.
100ug25 mg1000 tests100ug10 mg 5 G0.1 mg2.5 mg100ul100ul1 mg50 ug
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#33009631 2020/10/03
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Antioxidant and Antiglycation Activities of Syzygium paniculatum Gaertn and Inhibition of Digestive Enzymes Relevant to Type 2 Diabetes Mellitus.
Advanced glycation end-products (AGEs) may be a contributing factor in the development of diabetes-specific vascular pathologies that affect the retina, glomerulus and peripheral nerves. In this study, Australian native food plant species Syzygium paniculatum was investigated for activities relevant to Type 2 diabetes mellitus including inhibition of α-amylase, α-glucosidase and protein glycation. A methanolic extract of the leaves showed the strongest α-amylase inhibition (IC = 14.29 ± 0.82 μg/mL, p < 0.05) when compared with other extracts. For inhibition of α-glucosidase, the strongest inhibition was shown for the water, methanolic and acetone extracts of leaves with IC values ranging from 4.73 ± 0.96 to 7.26 ± 0.92 μg/mL. In the BSA-glucose model, fruit and leaf extracts inhibited formation of protein-bound fluorescent AGEs with IC values ranging between 11.82 ± 0.71 and 96.80 ± 13.41 μg/mL. Pearson's correlation analysis showed that the AGE inhibition significantly correlated with DPPH (r = -0.8964, p < 0.05) and ABTS (r = -0.8326, p < 0.05). α-amylase inhibitory activities strongly correlated with DPPH (r = -0.8964, p < 0.001). α-glucosidase inhibition strongly correlated with TPC (r = -0.9243, p < 0.05), FRAP (r = -0.9502, p < 0.01), DPPH (r = -0.9317, p < 0.01) and ABTS (r = -0.9486, p < 0.01). This study provides a strong rationale for further investigation aimed at isolating and identifying the active compounds responsible for the observed effects on targets relevant to diabetes.Sangseo Kim, Susan J Semple, Bradley S Simpson, Permal Deo