Search results for: METH1
#30649479 // To Up
Synergism of Adulticides and Insect Growth Regulators Against Larval Cat Fleas (Siphonaptera: Pulicidae).The use of topical and oral therapies on pets has revolutionized the control of cat fleas, Ctenocephalides felis felis (BouchÃ©). Herein, we tested the biological activity of two adulticides, fipronil and imidacloprid, and the insect growth regulators (IGRs), methoprene and pyriproxyfen. The LC50's of fipronil, imidacloprid, methoprene, and pyriproxyfen in larval rearing medium for second and third instars were 1.13, 0.73, 0.35, and 0.23 ppm, respectively. Combinations of imidacloprid and methoprene and pyriproxyfen were synergistic. The combination indices (CIs) at an effective dose (ED95) of imidacloprid:methoprene (Im:Meth) were 0.54, 0.44, 0.66, 0.73, and 0.62 for Im1:Meth1, Im5:Meth1, Im10:Meth1, Im20:Meth1, and Im40:Meth1, respectively. Similarly, the CIs of imidacloprid:pyriproxyfen (Im:Pyri) at an ED95 were 0.73 and 0.50 for Im1:Pyri1 and Im5:Pyri1, respectively. Combinations of fipronil:methoprene (Fip:Meth) provided variable results with Fip1:Meth1 being antagonistic (CI = 1.61). Combinations at 5:1, 10:1, and 20:1 at an ED95 were moderately synergistic. Combinations of Fip:Pyri at 1:1 were antagonistic at an ED95 with a CI of 2.87. When the combinations were reversed, neither the imidacloprid nor fipronil synergized either IGR. The dose response indices (DRI) for both Im:Meth and Im:Pyri indicate that the concentrations of the combinations could be significantly reduced and still be as effective as imidacloprid alone. Certain combinations of adulticides and IGRs were synergistic against immature fleas.
Michael K Rust, W Lance H Hemsarth
1230 related Products with: Synergism of Adulticides and Insect Growth Regulators Against Larval Cat Fleas (Siphonaptera: Pulicidae).5ug0.1 mg50 ug10 mg1 kit(96 Wells)100.00 ul0.1 mg1000 100.00 ul1 ml5ug100ug
#28605028 2017/08/25 To Up
A robust protocol for the isolation of cellular proteins from Xanthomonas campestris to analyze the methionine effect in 2D-gel experiments.Two-dimensional PAGE (2D-PAGE) is a key technique for the separation of complex protein samples to survey the protein inventory of prokaryotic microorganisms. Although the preparation of proteins is a critical step in 2D gel experiments, its effect on the outcome of proteome data is often underestimated. In this work, we show that the choice of protein isolation method can have a profound impact on the quality of 2D gels of protein extracts from prokaryotes. Based on Xanthomonas campestris, a commercially relevant producer of the thickening agent xanthan, we validated a phenol extraction protocol for the purification of bacterial proteins that provides excellent 2D gel separation. As a proof of concept, this method was used to study the effect of methionine-a medium compound that reduces the xanthan output of industrial fermentations-on the cellular proteome of Xanthomonas. The detection of nine regulated proteins associated with sulfur metabolism (Cgl, CysI, CysJ, CysK, MetH1, MetY) and sugar nucleotide biosynthesis (Pgi, Ugd, XanA) proved the efficiency of phenol extraction for the screening of statistically significant abundance changes in 2D gel spots and MALDI-TOF-MS based identification in bacteria. Since this method is very robust, it may be useful for the study of other prokaryotes that are relevant in industrial biotechnology.
Fabian Schulte, Markus Hardt, Karsten Niehaus
1372 related Products with: A robust protocol for the isolation of cellular proteins from Xanthomonas campestris to analyze the methionine effect in 2D-gel experiments.1100 100 units12 Pieces/Box
#19614921 // To Up
Adenovirus-mediated METH1 gene expression inhibits hypertrophic scarring in a rabbit ear model.Hypertrophic scarring remains a major problem for patients who have suffered from surgeries or burns. Vascularization plays an important role in the early phase of hypertrophic scarring. Therefore, the inhibition of angiogenesis might be used as a preventive strategy. In this study, we assessed the effect of anti-angiogenesis resulting from adenovirus-mediated METH1 (metalloprotease and thrombospondin1) gene expression on the hypertrophic scar formation in a rabbit ear model of hypertrophic scarring. We first investigated the number of microvessel and microcirculatory perfusion in untreated scars on days 10, 30, 60, and 90 after epithelialization. Then, we examined the effect of anti-angiogenesis by adenovirus-mediated METH1 expression on hypertrophic scar formation by calculating the scar elevation index, counting the microvessel and argyrophilic nucleolar organizer region particle, and detecting the amount of collagen on days 30 and 60 after treatment. We found that untreated scar tissues at the proliferative phase (days 10-60 after epithelialization) had a significantly higher density of microvessel and microcirculatory perfusion than those at the mature phase (day 90 after epithelization) (both p<0.05). On days 30 and 60 after treatment, the hypertrophic scar formation was significantly inhibited in the treatment group. There was significantly reduced scar elevation index, microvessel count, number of argyrophilic nucleolar organizer region, and total collagen content for treated scars. Our results demonstrate that METH1 has a markedly inhibitive effect on the formation of hypertrophic scar, and may thus have a promising application in the prevention of human hyperthropic scars.
Baoqiang Song, Wei Zhang, Shuzhong Guo, Yan Han, Yang Zhang, Fucheng Ma, Linxi Zhang, Kaihua Lu
2719 related Products with: Adenovirus-mediated METH1 gene expression inhibits hypertrophic scarring in a rabbit ear model.300 units100ug Lyophilized100ug Lyophilized100ug Lyophilized100 100ug Lyophilized100ug100ug100ug Lyophilized100ug100ug Lyophilized100ug Lyophilized
#18590222 // To Up
[Effect of METH1 gene transfection on the proliferation of rabbit's ear scar].To investigate the effect of METH1 gene transfection on fibroblast proliferation and I, III collagen synthesis in rabbit ear scar.
Bao-Qiang Song, Kai-Hua Lu, Shu-Zhong Guo, Yang Zhang, Pai Peng, Fu-Cheng Ma, Hui-Yuan Li
1921 related Products with: [Effect of METH1 gene transfection on the proliferation of rabbit's ear scar].100ul 100ul50 ug 100ul100ug 100ul 100ul100ug 100ul 100ul100ug Lyophilized
#18361243 // To Up
[Angiogenesis in hypertrophic scar of rabbit ears and effect of extracellular protein with metalloprotease and thrombospondin 1 domains on hypertrophic scar].To investigate the angiogenesis in hypertropic scar tissue of rabbit ears at different periods and to explore a new method to prevent hyperplastic scar.
Baoqiang Song, Kaihua Lu, Yang Zhang, Shuzhong Guo, Yan Han, Fucheng Ma, Huiyuan Li
1041 related Products with: [Angiogenesis in hypertrophic scar of rabbit ears and effect of extracellular protein with metalloprotease and thrombospondin 1 domains on hypertrophic scar].1000 TESTS/0.65ml100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ul100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized
#17105914 // To Up
Methamphetamine, morphine, and their combination: acute changes in striatal dopaminergic transmission evaluated by microdialysis in awake rats.The co-administration of methamphetamine (METH) and MOR (MOR)-like compounds is becoming increasingly popular among drug abusers. Recently, it was demonstrated that rats would self-inject METH-heroin combination and that this combination produced a greater rewarding effect than the identical doses of METH alone and it was further suggested that enhanced reward might underlie the popularity of this combination. However, there is null information on the effects of the MOR-METH combination on striatal dopaminergic transmission. In the present article, in vivo brain microdialysis was used to examine the effects of two METH doses (1 and 5 mg/kg, i.p.; [METH1: hyperlocomotion-inducing] and [METH5: stereotypy-inducing], respectively) and MOR (10 mg/kg, i.p. [MOR10]) either alone or in combination on dopamine (DA) and 3,4-dihydroxyphenylacetic acid (DOPAC) release in caudate putamen (CPu) in freely moving rats. METH1 evoked a transient threefold increase in DA overflow in only one-third of dosed rats. On the contrary, METH5 elicited a 11-fold increase in the extracellular DA levels 30 min after dosing and stayed significantly (P < 0.05) above control levels up to 1.5 h. On the other hand, MOR10 did not significantly change DA extracellular levels. MOR10-METH1 combination prolonged DA outflow for 1 h in all rats dosed without changing peak effect compared to METH1. On the other hand, MOR10-METH5 combination did not change the peak effect nor the DA outflow profile compared to METH5 alone. Consistently, there is a concentration-dependent decrease in DOPAC efflux evoked by METH: METH1 evoked a smaller decrease in DOPAC outflow showing a tendency for returning to basal values whereas METH5 kept DOPAC extracellular levels reduced throughout the experiment. Again, MOR10 did not significantly change DOPAC extracellular levels. MOR delayed the onset without changing METH effect on the DOPAC output. These findings provide suggestive evidence that MOR potentiated the increase in extracellular DA levels induced by a low dose of METH. Thus, this combination yields a profile of action that might underlie the reinforcing properties sought by drug addicts.
Frederico C Pereira, Elita LourenÃ§o, Nuno Milhazes, Teresa Morgadinho, Carlos F Ribeiro, Syed F Ali, Tice R Macedo
1224 related Products with: Methamphetamine, morphine, and their combination: acute changes in striatal dopaminergic transmission evaluated by microdialysis in awake rats.100ug Lyophilized100 100ug Lyophilized1 mg100ug20 ul (10 mM)100ug100 μg1 Set
#15449629 // To Up
[Cloning of human angiogenesis inhibitor METH1 and its expression in mammalian cells].To get the full length of human METH1 cDNA and express it steadily in mammalian cell stably.
Yang Zhang, Kai-hua Lu, Shu-zhong Guo, Ge Song, Guo-rong Yang, Zhen Wang, Yong-hong Lei
2210 related Products with: [Cloning of human angiogenesis inhibitor METH1 and its expression in mammalian cells].50ug50ug100μg5ug100uL10mg10 rxns10 4 Arrays/Slide0.5 mg1mg
#12814950 // To Up
ADAMTS1: a matrix metalloprotease with angioinhibitory properties.Neovascularization is a hallmark of cancer progression. Suppression of the angiogenic response in tumors has been associated with inhibition and even regression of total tumor mass. Therefore, the derivation of synthetic or natural products that could interfere with proangiogenic signaling pathways can greatly impact cancer therapy. Using the antiangiogenic motifs in thrombospondin-1, we have recently cloned METH1/ADAMTS1, a secreted metalloproteinase with three thrombospondin-1, and shown that the protein inhibits endothelial cell proliferation in vitro and blocks the neovascular response induced by growth factors in vivo. The mechanism of action responsible for these events has not been elucidated. In this report, we present evidence to support two effects of METH1/ADAMTS1 that impact proangiogenic signaling events. ADAMTS1 binds to VEGF and dampens VEGFR2 phosphorylation. The ability of ADAMTS1 to bind VEGF and functionally inactivate VEGFR2 is reversible as dissociation of the complex results in active growth factor. A second activity of ADAMTS1 requires the catalytic domain as a single point mutation in the metalloproteinase domain renders the protein inactive in tumor xenograft assays. The emerging theme is that both domains are likely required for the antiangiogenic/antitumor activities of ADAMTS1.
M Luisa Iruela-Arispe, Darren Carpizo, Alfonso Luque0.2 mg 96T/Kit 100 mg0.2 mg100 2 ml1 ml100 200 1 ml100 1 mL
#12716911 2003/04/25 To Up
ADAMTS1/METH1 inhibits endothelial cell proliferation by direct binding and sequestration of VEGF165.ADAMTS1 is a metalloprotease previously shown to inhibit angiogenesis in a variety of in vitro and in vivo assays. In the present study, we demonstrate that ADAMTS1 significantly blocks VEGFR2 phosphorylation with consequent suppression of endothelial cell proliferation. The effect on VEGFR2 function was due to direct binding and sequestration of VEGF165 by ADAMTS1. Binding was confirmed by co-immunoprecipitation and cross-linking analysis. Inhibition of VEGF function was reversible, as active VEGF could be recovered from the complex. The interaction required the heparin-binding domain of the growth factor, because VEGF121 failed to bind to ADAMTS1. Structure/function analysis with independent ADAMTS1 domains indicated that binding to VEGF165 was mediated by the carboxyl-terminal (CT) region. ADAMTS1 and VEGF165 were also found in association in tumor extracts. These findings provide a mechanism for the anti-angiogenic activity of ADAMTS1 and describe a novel modulator of VEGF bioavailability.
Alfonso Luque, Darren R Carpizo, M Luisa Iruela-Arispe
2922 related Products with: ADAMTS1/METH1 inhibits endothelial cell proliferation by direct binding and sequestration of VEGF165.0.1 mg1.00 flask100ul25 Tests1.00 flask96 rxns0.2 mL1.00 flask100 ml. 6 ml Ready-to-use
#12049787 // To Up
Expression of ADAMTS1 during murine development.ADAMTS1/METH1 belongs to the ADAMTS (a disintegrin and metalloprotease with thrombospondin repeats) family of proteins that currently comprises 18 members. Targeted inactivation of the ADAMTS1 gene results in morphological defects in the kidney, adrenal gland, and adipose tissue in addition to growth retardation and infertility in females. To gain further insight on the biology of ADAMTS1, we examined its expression pattern in the developing mouse from embryonic day 10 (E10) to E18. Expression analysis by RNase protection assays revealed detectable levels of ADAMTS1 transcripts in E10-E18 yolk sac, placenta, brain, heart, lung, limb bud, liver, spleen, and kidney, with much lower levels in the adult. Using in situ hybridization, we have localized ADAMTS1 transcripts predominantly to the epithelium of the developing lung, pancreas, kidney and to a subset of neurons in a temporally restricted manner. Expression was also detected in the tunica media of the aorta, pulmonary, and hepatic vessels.
Shelley N-M Thai, M Luisa Iruela-Arispe300 units1 mg1 mg1 mg25 μg10 μg20 ìg50 ug1 mg
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45 Fax 0032 16 50 90 45
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50 Fax 01 43 25 01 60
52062 Aachen Deutschland
Tel 0241 40 08 90 86 Fax 0241 55 91 05 36
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
Schweiz Züri +41435006251
Česká republika Praha +420246019719
Ireland Dublin +35316526556
Norge Oslo +4721031366
Finland Helsset +358942419041
Sverige Stockholm +46852503438
Ελλάς Αθήνα +302111768494
Magyarország Budapest +3619980547
GENTAUR Poland Sp. z o.o.
ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
Tel 058 710 33 44
Fax 058 710 33 48
GENTAUR Nederland BV
5521 DG Eersel Nederland
Tel 0208-080893 Fax 0497-517897
Piazza Giacomo Matteotti, 6, 24122 Bergamo
Tel 02 36 00 65 93 Fax 02 36 00 65 94
53 Iskar Str. 1191 Kokalyane, Sofia