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#32745904   2020/07/25 To Up

Co-grinding with surfactants as a new approach to enhance in vitro dissolution of praziquantel.

This paper evaluates the process of co-grinding with a surfactant as a new approach to enhance physicochemical and biopharmaceutical properties of praziquantel (PZQ), a poorly soluble drug that is essential for the treatment of schistosomiasis, a neglected tropical disease. Surfactants used in this study were poloxamer F-127 and sucrose stearate (C-1816), selected based on their well-documented biocompatibility and solubilizing activity. A series of products were prepared by mechanochemical activation using vibrational ball-mill at different drug to surfactant ratio and milling times. The obtained products were characterised in terms of drug recovery, solubility and in vitro dissolution rates. The obtained results were correlated to solid-state properties of the products analysed by differential scanning calorimetry, powder X-ray diffraction and particle size analysis. Results of UPLC-MS analysis and H-NMR spectroscopy showed that the used surfactants and applied grinding procedures caused no chemical degradation of the PZQ. The physicochemical properties, solubility and the in vitro dissolution enhancement of the co-ground products were related to the drug to surfactant ratio and the grinding protocol applied. The highest enhancement of the in vitro dissolution rate was achieved at the drug to surfactant ratio of 10:3 and 10:2 for F-127 and C-1816, respectively with the milling time of 30 min. The MTT assay on Caco-2 cell line demonstrated the biocompatibility of both co-ground products. Furthermore, the surfactants used did not change intrinsically high intestinal permeability of PZQ (Papp ∼ 4.00 × 10 cm s). The presented results confirmed that the co-grinding with surfactant is a promising new approach in enhancing in vitro dissolution of poorly soluble drugs like PZQ.
Alessio Gaggero, Bisera Jurišić Dukovski, Irena Radić, Ivana Šagud, Irena Škorić, Dominik Cinčić, Mario Jug

2209 related Products with: Co-grinding with surfactants as a new approach to enhance in vitro dissolution of praziquantel.

96 tests1 kit(96 Wells)1 kit1 kit1 kit(96 Wells)1 kit24 tests1 kit(96 Wells)100 assays100 assays1 kit

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#32745631   2020/07/31 To Up

Differential capacity of CD90+ cells in autophagy activation following chemotherapy in hepatocellular carcinoma.

Analysis of cancer biomarkers is an important tool in developing targeted-therapy and in modulating chemoresistance. Here, we analyze the relevance of CD90, a marker of cancer stem cells (CSC) in hepatocellular carcinoma (HCC) and its correlation with autophagy.
Huy Q Do, An B Luong, Deborah Bonazza, Cristina Bottin, Thao Pt Doan, Long Dc Tran, Nhung H Truong, Gianluca Tell, Hoa Lt Pham, Claudio Tiribelli, Caecilia Hc Sukowati

2545 related Products with: Differential capacity of CD90+ cells in autophagy activation following chemotherapy in hepatocellular carcinoma.

96 tests

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#32745158   2020/07/31 To Up

Anti proliferative and apoptotic effects on pancreatic cancer cell lines indicate new roles for ANGPTL8 (Betatrophin).

Despite considerable advances, the treatment of pancreatic cancer (PC) still requires much effort. Unusual regulation of the Wnt and apoptotic signaling pathways is widespread in cancer incidence. For instance, the WIF1 (Wnt inhibitory factor 1) gene is down-regulated in many cancers. The purpose of this study was to determine the effects of recombinant Betatrophin, a recently discovered hormone, on MiaPaca-II and Panc-1 pancreatic cell lines. Various concentrations of Betatrophin were added to MiaPaca-II and Panc-1 pancreatic cell lines during periods of 24 , 48, and 72 h. The MTT assay was applied to investigate cell proliferation after treatment. The rate of apoptotic cells was assessed using double-staining flow cytometry, and the expression levels of the WIF1 gene and Bcl2 protein was observed by real-time PCR and western blotting, respectively. The findings of this study suggest that Betatrophin has an anti-proliferative effect on both MiaPaca-II and Panc-1 cell lines, in line with the up-regulation of WIF1 as a tumor suppressor gene. Moreover, the induction of apoptosis by ANGPTL8 occurred by the down-regulation of Bcl2. Thus, Betatrophin can be proposed as a potential therapeutic drug for treating pancreatic cancer.
Fatemeh Taherkhani, Kamran Mousavi Hosseini, Sanaz Zebardast, Koorosh Goodarzvand Chegini, Nematollah Gheibi

2472 related Products with: Anti proliferative and apoptotic effects on pancreatic cancer cell lines indicate new roles for ANGPTL8 (Betatrophin).

96T100ul100ul10 plates100ug Lyophilized

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#32745124   2020/08/03 To Up

Effective inhibition of cancer cells by recombinant adenovirus expressing EGFR-targeting artificial microRNA and reversed-caspase-3.

The EGFR-targeting cancer therapies are commonly facing drug resistance, mostly due to mutations. Gene therapy with artificial microRNA targeting EGFR conserved sequence may avoid such problem. In this study, we constructed a recombinant adenovirus expressing EGFR-targeting artificial microRNA and active revCASP3 (Ad-EC), under the control of tumor-specific SLPI promoter, and evaluated its inhibitory effect on HEP-2 cancer cells both in vitro and in vivo. MTT assay showed that cell growth inhibition rate at 72h was 44.0% in Ad-EC group at MOI 50, while the rate was 7.7% in the control virus Ad-GFP group and 3.6% in Cetuximab (500 μg/ml) group respectively. Flow cytometry analysis revealed the late apoptotic cells rate was 36.1% in Ad-EC group, significantly higher than 6.5% of Ad-GFP group (p < 0.001). When Ad-EC (MOI 50) was combined with CDDP (0.25 μg/ml), late apoptotic cells rate increased to 61.2%, significantly higher than each monotherapy group (P < 0.001). The real-time xCELLigence system recorded an effective cell growth inhibition in Ad-EC and CDDP groups, and more enhanced effect in Ad-EC plus CDDP group. Western blot revealed that Ad-EC could inhibit the activation of AKT pathway and ERK1/2 pathway, while Cetuximab had the AKT pathway over-activated. In vivo experiments with HEP-2 xenograft in nude mice confirmed the tumor inhibition in Ad-EC, CDDP and Ad-EC plus CDDP groups compared with PBS group (P < 0.01). Collectively, these data support the effective inhibition of cancer cells by this novel gene therapy strategy.
Maoxiao Yan, Jia Chen, Hua Jiang, Yuqiong Xie, Chunchun Li, Lihong Chen, Beibei Yang, Jiang Cao

1917 related Products with: Effective inhibition of cancer cells by recombinant adenovirus expressing EGFR-targeting artificial microRNA and reversed-caspase-3.

25 units201.00 flask100100100 units1.00 flask25 units96T100 units100 units1.00 flask

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#32744980   2020/07/29 To Up

Epigenetic Evaluation of N-(2-hydroxyphenyl)-2-propylpentanamide, a Valproic Acid Aryl Derivative with activity against HeLa cells.

Valproic acid (VPA) is an HDAC inhibitor (HDACI) with anticancer activity, but it is hepatotoxic. N-(2-hydroxyphenyl)-2-propylpentanamide (o-OH-VPA) is a VPA aryl derivative designed in silico as a selective inhibitor of HDAC8 with biological properties against HeLa, rhabdomyosarcoma and breast cancer cell cultures.
Luna-Palencia Gabriela Rebeca, Correa-Basurto José, Trujillo-Ferrara José, Meraz-Ríos Marco Antonio, Vásquez-Moctezuma Ismael

2106 related Products with: Epigenetic Evaluation of N-(2-hydroxyphenyl)-2-propylpentanamide, a Valproic Acid Aryl Derivative with activity against HeLa cells.

200 mg5 g5 mg25 mg

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#32744697   // To Up

LncRNA DSCAM-AS1 promoted cell proliferation and invasion in osteosarcoma by sponging miR-101.

Long noncoding RNAs (lncRNAs) play critical roles in osteosarcoma (OS) progression. LncRNA DSCAM-AS1 has been reported to function as a tumor promoter in various cancers. However, the potential mechanism of DSCAM-AS1 in OS remains rarely know.
C-L Yu, N-W Xu, W Jiang, H Zhang, Y Ma

1750 related Products with: LncRNA DSCAM-AS1 promoted cell proliferation and invasion in osteosarcoma by sponging miR-101.

96 samples96 samples96 tests24 tests96 samples100 ml.24 tests25 ml.96 samples24 tests24 tests0.5 mg

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#32744686   // To Up

LncRNA SNHG6 regulating Hedgehog signaling pathway and affecting the biological function of gallbladder carcinoma cells through targeting miR-26b-5p.

Long-chain non-coding RNA (LncRNA) is abnormally expressed in various malignant tumors. In recent years, it has been found that the expression of LncRNA SNHG6 is upregulated in gallbladder carcinoma tissues, which participated in the occurrence and development of gallbladder carcinoma. However, the clinical value of SNHG6 in gallbladder cancer serum is not clear, and there are few studies regulating the biological function of gallbladder carcinoma cells. This study aimed to investigate LncRNA SNHG6 and miR-26b-5p in gallbladder carcinoma and its related mechanisms.
X-F Liu, K Wang, H-C Du

2007 related Products with: LncRNA SNHG6 regulating Hedgehog signaling pathway and affecting the biological function of gallbladder carcinoma cells through targeting miR-26b-5p.

1.5x10(6) cells1.5 x 10^6 cellsInhibitors2 Pieces/Box8 inhibitors2 Pieces/Box500 reactions14 inhibitors2 Pieces/Box1.5 x 10^6 cells2 Pieces/Box1.5x10(6) cells

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#32744455   2020/08/03 To Up

The neutral red assay can be used to evaluate cell viability during autophagy or in an acidic microenvironment in vitro.

Harsh conditions within the tumor microenvironment, such as hypoxia and extracellular acidic pH (pH), inactivate some chemotherapies, which results in limited or no cytotoxicity. Standard MTT, ATPlite and protease assays that are used to determine the potency of newly developed drugs often give erroneous results when applied under hypoxic or acidic conditions. Therefore, development of a cytotoxicity assay that does not yield false positive or false negative results under circumstances of both hypoxia and acidic pH is needed. We evaluated currently used cell viability assays as well as neutral red staining to assess viability of ovarian and pancreatic cancer cells grown in an acidic pH microenvironment after treatment with carboplatin, gemcitabine or chloroquine. We validated cell viability using western blotting of pro-caspase-9 and cleaved-caspase-9, and LC3-I and - II. Standard cell viability assays indicated cell viability accurately at pH 7.4, but was not correlated with induction of apoptosis or autophagy at acidic pH. By contrast, our modified neutral red assay detected cell viability accurately over a range of pH as demonstrated by its correlation with induction of apoptosis and autophagy. Neutral red staining is effective for evaluating the effect of chemotherapeutic agents on cell viability under acidic pH or hypoxic conditions.
Jorge G Gomez-Gutierrez, Neal Bhutiani, Molly W McNally, Phillip Chuong, Wenyuan Yin, Meredith A Jones, Matthew R Zeiderman, William E Grizzle, Lacey R McNally

2358 related Products with: The neutral red assay can be used to evaluate cell viability during autophagy or in an acidic microenvironment in vitro.

96 tests1 kit

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#32744344   2020/08/03 To Up

Protective effect of Lycium barbarum polysaccharide on ethanol-induced injury in human hepatocyte and its mechanism.

The purpose of this research is to study the effect of Lycium barbarum polysaccharide on ethanol-induced liver injury and its mechanism. The cell survival rate, the apoptosis rate, and the intracellular ROS level was detected by MTT assay, flow cytometry, laser confocal microscopy, and fluorescence spectrophotometry, respectively. The antioxidative indices were determined by ELISA kits and the protein level was detected by western blot. The result showed Lycium barbarum polysaccharide could protect ethanol-induced cell injury by reducing cell apoptosis and regulating the levels of indicators related to oxidative stress, such as ROS, MDA, SOD, etc. In addition, LBP could increase the nuclear expression of Nrf2 protein and significantly up-regulate the expression levels of Nrf2 protein and its downstream proteins, such as HO-1, NQO1, and GCLC in the cell nucleus. Therefore, Lycium barbarum polysaccharide has a protective effect on ethanol-induced liver cell injury and it plays the role in cell apoptosis pathway and oxidative stress pathway. PRACTICAL APPLICATIONS: Lycium barbarum is a kind of food that can be used as food and medicine in China. The result showed that Lycium barbarum polysaccharide could protect ethanol-induced liver cell injury, which is beneficial to the application of LBP in functional food.
Jianteng Wei, Linghao Zhang, Jianfei Liu, Dong Pei, Ningli Wang, Han Wang, Duolong Di, Yewei Liu

1869 related Products with: Protective effect of Lycium barbarum polysaccharide on ethanol-induced injury in human hepatocyte and its mechanism.

100ul100 ul5ug50 ul96T100 μg100 μg100 ul2ug4 Membranes/Box100ug Lyophilized100 μg

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#32744323   2020/08/03 To Up

Lnc RNA ZFAS1 regulates the proliferation, apoptosis, inflammatory response and autophagy of fibroblast-like synoviocytes via miR-2682-5p/ADAMTS9 axis in Rheumatoid Arthritis.

Rheumatoid arthritis (RA) is a frequent autoimmune disease. Emerging evidence indicated that ZNFX1 antisense RNA1 (ZFAS1) participates in the physiological and pathological processes in RA. However, knowledge of ZFAS1 in RA is limited, the potential work pathway of ZFAS1 needs to be further investigated.
Shanshan Yang, Wei Yin, Yan Ding, Fan Liu

2862 related Products with: Lnc RNA ZFAS1 regulates the proliferation, apoptosis, inflammatory response and autophagy of fibroblast-like synoviocytes via miR-2682-5p/ADAMTS9 axis in Rheumatoid Arthritis.

100ul200ul100 ug100 ml.2 Pieces/Box100μg1100ul200ul 100 UG

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