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#33631785   2021/02/23 To Up

Biophysical and rheological biomarkers of red blood cell physiology and pathophysiology.

This review summarizes the significant biophysical and rheological aspects of red blood cell physiology and pathophysiology in relation to recent advances in microfluidic biomarker assays and emerging targeted or curative intent therapies.
Umut A Gurkan

1196 related Products with: Biophysical and rheological biomarkers of red blood cell physiology and pathophysiology.

25ml100 mg100ml100 extractions 25 MG100ug25 mg10 mg50ml100ug50 mg

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#33631768   2021/02/25 To Up

Fecal lactoferrin predicts primary non-response to biologic agents in inflammatory bowel disease.

Fecal Lactoferrin (FL) is a timely and accurate marker of inflammation in ulcerative colitis (UC) and Crohn's disease (CD). Aim of this study was to verify whether FL can predict primary non-response (PNR) to biologic agents during induction.
Dario Sorrentino, Vu Q Nguyen, Kim Love

2087 related Products with: Fecal lactoferrin predicts primary non-response to biologic agents in inflammatory bowel disease.

100 1 mg100ug100ug 100 UG1-99 mg/ml/ea price x 2100ug100ug

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#33631698   2021/02/11 To Up

Multibiomarker-based assessment of toxicity of central European strains of filamentous cyanobacteria Aphanizomenon gracile and Raphidiopsis raciborskii to zebrafish Danio rerio.

The global increase in cyanobacterial blooms poses environmental and health threats. Selected cyanobacterial strains reveal toxicities despite a lack of synthesis of known toxic metabolites, and the mechanisms of these toxicities are not well understood. Here we investigated the toxicity of non-cylindrospermopsin and non-microcystin producing Aphanizomenon gracile and Raphidiopsis raciborskii of Central European origin to zebrafish exposed for 14 days to their extracts. Toxicological screening revealed the presence of anabaenopeptins and a lack of anatoxin-a, ß-methylamino-L-alanine or saxitoxins in examined extracts. The responses were compared to 20 μg L of common cyanobacterial toxins cylindrospermopsin (CYN) and microcystin-LR (MC-LR). The expression of the marker genes involved in apoptosis (caspase 3a and 3b, Bcl-2, BAX, p53, MAPK, Nrf2), DNA damage detection and repair (GADD45, RAD51, JUN, XPC), detoxification (CYP1A, CYP26, EPHX1), lipid metabolism (PPARa, FABP1, PLA2), phosphorylation/dephosphorylation (PPP6C, PPM1) and cytoskeleton (actin, tubulin) were examined using targeted transcriptomics. Cellular stress and toxicity biomarkers (oxidative injury, antioxidant enzymes, thiol pool status, and lactate dehydrogenase activity) were measured in the liver, and acetylcholinesterase activity was determined as an index of neurotoxicity in the brain. The extracts of three cyanobacterial strains that produce no known cyanotoxins caused marked toxicity in D. rerio, and the biomarker profiles indicate different toxic mechanisms between the bioactive compounds extracted from these strains and the purified cyanotoxins. All studied cyanobacterial extracts and purified cyanotoxins induced oxidative stress and neurotoxicity, downregulated Nrf2 and CYP26B1, disrupted phosphorylation/dephosphorylation processes and actin/tubulin cytoskeleton and upregulated apoptotic activity in the liver. The tested strains and purified toxins displayed distinctively different effects on lipid metabolism. Unlike CYN and MC-LR, the Central European strain of A. gracile and R. raciborskii did not reveal a genotoxic potential. These findings help to further understand the ecotoxicological consequences of toxic cyanobacterial blooms in freshwater ecosystems.
Halina Falfushynska, Oksana Horyn, Inna Osypenko, Piotr Rzymski, Łukasz Wejnerowski, Marcin K Dziuba, Inna M Sokolova

2857 related Products with: Multibiomarker-based assessment of toxicity of central European strains of filamentous cyanobacteria Aphanizomenon gracile and Raphidiopsis raciborskii to zebrafish Danio rerio.

5 G101 module10 mgOne 96-Well Microplate Ki1 module100ug 50 UG5 mg1 kit(96 Wells)1 ml

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#33631688   2021/01/22 To Up

Cytogenetic toxicity from pesticide and trace element mixtures in soils used for conventional and organic crops of Allium cepa L.

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Jorge L Gallego, Jesus Olivero-Verbel

1074 related Products with: Cytogenetic toxicity from pesticide and trace element mixtures in soils used for conventional and organic crops of Allium cepa L.

200ug2.5 mg10 mg1000 tests100ug200ul50 ug 25 mg 5 G10 mg10 mg

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#33631685   2021/02/09 To Up

Performance of faecal indicator bacteria, microbial source tracking, and pollution risk mapping in tropical water.

Faecal indicator bacteria (FIB) are used for the assessment of faecal pollution and possible water quality deterioration. There is growing evidence that FIB used in temperate regions are not adequate and reliable to detect faecal pollution in tropical regions. Hence, this study evaluated the adequacy of FIB, including total coliforms (TC), Escherichia coli (EC), Enterococci (IEC), and Clostridium perfringens (CP) in the high-altitude, tropical country of Ethiopia. In addition to FIB, for microbial source tracking (MST), a ruminant-associated molecular marker was applied at different water types and altitudes, and faecal pollution risk mapping was conducted based on consensus FIB. The performances of the indicators were evaluated at 22 sites from different water types. The results indicate that EC cell enumeration and CP spore determination perform well for faecal contamination monitoring. Most of the sub-basins of Lake Tana were found to be moderately to highly polluted, and the levels of pollution were demonstrated to be higher in the rainy season than in the post-rainy season. Markers associated with ruminants (BacR) were identified in more than three quarters of the sites. A bacterial pollution risk map was developed for sub-basins of Lake Tana, including the un-gauged sub-basins. We demonstrate how bacterial pollution risk mapping can aid in improvements to water quality testing and reduce risk to the general population from stream bacteria.
Goraw Goshu, A A Koelmans, J J M de Klein

2741 related Products with: Performance of faecal indicator bacteria, microbial source tracking, and pollution risk mapping in tropical water.

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#33631684   2021/02/11 To Up

Biomarkers of phthalates and alternative plasticizers in the Flemish Environment and Health Study (FLEHS IV): Time trends and exposure assessment.

Restrictions on the use of legacy phthalate esters (PEs) as plasticizer chemicals in several consumer products has led to the increased use of alternative plasticizers (APs), such as di-(iso-nonyl)-cyclohexane-1,2-dicarboxylate (DINCH) and di-(2-ethylhexyl) terephthalate (DEHTP). In the fourth cycle of the Flemish Environment and Health Study (FLEHS IV, 2016-2020), we monitored exposure to seven PEs (diethyl phthalate (DEP), di-(2-ethylhexyl) phthalate (DEHP), di-isobutyl phthalate (DiBP), di-n-butyl phthalate (DnBP), butylbenzyl phthalate (BBzP, di-isononyl phthalate (DINP), and di-isodecyl phthalate (DIDP))and three APs (DINCH, DEHTP, and di-(2-ethylhexyl) adipate (DEHA)) by measuring multiple biomarkers in urine of 416 adolescents from Flanders, Belgium (14-15 years old). The reference values show that exposure to PEs is still widespread, although levels of several PE metabolites (e.g., sum of DEHP metabolites, mono-normal-butyl phthalate (MnBP) and mono-benzyl phthalate (MBzP)) have decreased significantly compared to previous human biomonitoring cycles (2003-2018). On the other hand, metabolites of DINCH and DEHTP were detected in practically every participant. Concentrations of AP exposure biomarkers in urine were generally lower than PE metabolites, but calculations of estimated daily intakes (EDIs) showed that exposure to DINCH and DEHTP can be considerable. However, preliminary risk assessment showed that none of the EDI or urinary exposure levels of APs exceeded the available health-based guidance values, while a very low number of participants had levels of MiBP and MnBP exceeding the HBM value. Several significant determinants of exposure could be identified from multiple regression models: the presence of building materials containing PVC, ventilation habits, socio-economic status and season were all associated with PE and AP biomarker levels. Cumulatively, the results of FLEHS IV show that adolescents in Flanders, Belgium, are exposed to a wide range of plasticizer chemicals. Close monitoring over the last decade showed that the exposure levels of restricted PEs have decreased, while newer APs are now frequently detected in humans.
Michiel Bastiaensen, Celine Gys, Ann Colles, Govindan Malarvannan, Veerle Verheyen, Gudrun Koppen, Eva Govarts, Liesbeth Bruckers, Bert Morrens, Carmen Francken, Elly Den Hond, Greet Schoeters, Adrian Covaci

2107 related Products with: Biomarkers of phthalates and alternative plasticizers in the Flemish Environment and Health Study (FLEHS IV): Time trends and exposure assessment.

100ul1 mg1,000 tests200ug100ug2.5 mg25 mg1000 tests10 mg50 ug 5 G

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#33631674   2021/02/22 To Up

EZH2-mediated epigenetic suppression of SH3BGRL potently inhibits lung cancer progression.

Lung cancer is the leading cause of cancer-related deaths worldwide. Lung cancer stem cells (CSCs) are a small population of cancer cells with self-renewal, therapeutic resistance, and tumor relapse capability. Yet the molecular mechanisms underlying lung CSCs self-renewal remain largely unknown. Here, we report that SH3BGRL were down-regulated in lung cancer tissues comparing with normal lung tissues and SH3BGRL low expression was correlated with the poor clinical outcomes of patients with lung cancer. Moreover, SH3BGRL was also weakly expressed in lung CSCs compared with its corresponding lung cancer cells. We first characterize that EZH2 directly binds to SH3BGRL promoter and transcriptional represses SH3BGRL expression in epigenetic level. Functionally, overexpression of SH3BGRL potently suppresses Lung CSCs self-renewal in vitro. The gain of function study reveals that SH3BGRL acts as a novel tumor suppressor via inhibiting lung cancer cell proliferation and migration as well as Lung CSCs self-renewal in vitro. Collectively, our work demonstrates that SH3BGRL hold potential as a favorable prognostic marker and therapeutic target for patients with lung cancer in future.
Zhiping Liu, Wen Xu, Xiansheng Tan, Chunhua Li

2311 related Products with: EZH2-mediated epigenetic suppression of SH3BGRL potently inhibits lung cancer progression.



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#33631664   2021/02/12 To Up

Lower limb biomechanics during gait in children with Achondroplasia.

Achondroplasia is an autosomal dominant disorder that has been reported to be the most common condition associated with severe disproportionate short stature. A limited number of studies exist in the literature reporting kinematic abnormalities in children with Achondroplasia. Reported deviations include increased pelvic tilt, flexed knees during stance and excessive hip abduction. However, studies lack full 3-dimensional data and results are variable. The aim of this study was to examine differences in 3-dimensional kinematics during gait between children with Achondroplasia and age-matched controls A retrospective analysis was conducted of our gait laboratory database over a 20-year period. Eight children with Achondroplasia and 32 children with typical development were included. A full 3-dimensional kinematic analysis was performed using a CODA mpx30/cx1 active marker system. Increased flexion at all joints in the sagittal plane, increased hip abduction and foot supination in the coronal plane and increased external hip rotation were present for children with Achondroplasia. In contrast to other studies, increased knee varus was not an issue for the children in this study. This study provides a full description of the kinematic patterns associated with children with Achondroplasia and, as such, a comprehensive baseline for planning rehabilitation strategies in these children.
Damien Kiernan

1529 related Products with: Lower limb biomechanics during gait in children with Achondroplasia.

50 1 mg1. Set100ug Lyophilized20 100 μg100 assays100 μg1 pce100 μg

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#33631651   2021/02/22 To Up

Clinical disease activity and flare in SLE: Current concepts and novel biomarkers.

Systemic lupus erythematosus (SLE) is a complex and heterogeneous systemic autoimmune disease associated with innate and adaptive immune dysregulation. SLE occurs primarily in females of childbearing age, with increased prevalence and severity in minority populations. Despite improvements in treatment modalities, SLE patients frequently experience periods of heightened disease activity and flare that can lead to permanent organ damage, increased morbidity, and early mortality. Such outcomes impair quality of life and inflict a significant socioeconomic burden. Predicting changes in SLE disease activity could allow for closer monitoring and preemptive treatment, but existing clinical, demographic and serologic markers have been only modestly predictive. Novel, proactive approaches to clinical disease management are thus critically needed. Panels of blood biomarkers can detect a breadth of immune pathway dysregulation that captures SLE heterogeneity and disease activity. Alterations in the balance of pro-inflammatory and regulatory soluble mediators have been associated with changes in clinical disease activity and are detectable several weeks prior to clinical flare occurrence. A soluble mediator score has been highly predictive of impending flare in both European American and African American SLE patients, and this score does not require a priori knowledge of specific pathway activation in the patient. We review current concepts of disease activity and flare in SLE, focusing on the potential of novel blood biomarkers to characterize and predict changes in disease activity. Measuring the disordered immune response in SLE in this way promises to improve disease management and prevent organ damage in SLE.
Aikaterini Thanou, Eldon Jupe, Mohan Purushothaman, Timothy B Niewold, Melissa E Munroe

2491 related Products with: Clinical disease activity and flare in SLE: Current concepts and novel biomarkers.



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#33631639   2021/02/05 To Up

On the quantitative effects of compression of retinal fundus images on morphometric vascular measurements in VAMPIRE.

This paper reports a quantitative analysis of the effects of joint photographic experts group (JPEG) image compression of retinal fundus camera images on automatic vessel segmentation and on morphometric vascular measurements derived from it, including vessel width, tortuosity and fractal dimension.

1130 related Products with: On the quantitative effects of compression of retinal fundus images on morphometric vascular measurements in VAMPIRE.

100ug 25 ml 1 g 100ul 2 ml 25 ml. 25 ml Ready-to-use 16 Arrays/Slide1 mg16 Arrays/Slide 6 ml

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