Search results for: Markers
#33098750 2020/10/24 To Up
Brachyury engineers cardiac repair competent stem cells.To optimize the regenerative proficiency of stem cells, a cardiopoietic protein-based cocktail consisting of multiple growth factors has been developed and advanced into clinical trials for treatment of ischemic heart failure. Streamlining the inductors of cardiopoiesis would address the resource intensive nature of the current stem cell enhancement protocol. To this end, the microencapsulated-modified-mRNA (M RNA) technique was here applied to introduce early cardiogenic genes into human adipose-derived mesenchymal stem cells (AMSCs). A single mesodermal transcription factor, Brachyury, was sufficient to trigger high expression of cardiopoietic markers, Nkx2.5 and Mef2c. Engineered cardiopoietic stem cells (eCP) featured a transcriptome profile distinct from pre-engineered AMSCs. In vitro, eCP demonstrated protective antioxidant capacity with enhanced superoxide dismutase expression and activity; a vasculogenic secretome driving angiogenic tube formation; and macrophage polarizing immunomodulatory properties. In vivo, in a murine model of myocardial infarction, intramyocardial delivery of eCP (600 000 cells per heart) improved cardiac performance and protected against decompensated heart failure. Thus, heart repair competent stem cells, armed with antioxidant, vasculogenic, and immunomodulatory traits, are here engineered through a protein-independent single gene manipulation, expanding the available regenerative toolkit.
Mark Li, Satsuki Yamada, Ao Shi, Raman Deep Singh, Tyler J Rolland, Ryounghoon Jeon, Natalia Lopez, Lukas Shelerud, Andre Terzic, Atta Behfar5 x 10A5 cells/vial10 x 50ul/Unit11 Plate of 96 x 20ul/Unit10 ug1 x 10^6 cells/vial5 x 200ul/Unit100|uI x 10 vials5 x 200ul/Unit100ìl x 10 vials75 x 50ul/Unit10 rxns
#33098737 2020/10/24 To Up
Alterations in Plasma Kynurenine Pathway Metabolites in Children and Adolescents with Bipolar Disorder and Unaffected Offspring of Bipolar Parents: A preliminary study.There has been growing scientific evidence in recent years that bipolar disorder (BD) is associated with alterations in the kynurenine (KYN) pathway. However, many of these studies have been limited by their focus on adults. Thus, this preliminary study investigated differences in the peripheral levels of KYN metabolites in children and adolescents with BD, unaffected offspring of parents with BD, and healthy controls (HCs).
Deborah Benevenuto, Kirti Saxena, Gabriel R Fries, Samira S Valvassori, Ramandeep Kahlon, Johanna Saxena, Sherin Kurian, Cristian P Zeni, Iram Kazimi, Giselli Scaini, Jair C Soares, João Quevedo
1775 related Products with: Alterations in Plasma Kynurenine Pathway Metabolites in Children and Adolescents with Bipolar Disorder and Unaffected Offspring of Bipolar Parents: A preliminary study.100 μg2 Pieces/Box2 Pieces/Box96T2 Pieces/Box2 Pieces/Box2 Pieces/Box2 Pieces/Box2 Pieces/Box2 Pieces/Box2 Pieces/Box2 Pieces/Box
#33098723 2020/10/24 To Up
HDL cholesterol protects from liver injury in mice with intestinal specific LXRα activation.Liver X receptors (LXRs) exert anti-inflammatory effects even though their hepatic activation is associated with hypertriglyceridemia and hepatic steatosis. Selective induction of LXRs in the gut might provide protective signal(s) in the aberrant wound healing response that induces fibrosis during chronic liver injury, without hypertriglyceridemic and steatogenic effects.
Irene Pierantonelli, Gessica Lioci, Fabio Gurrado, Debora Maria Giordano, Chiara Rychlicki, Claudia Bocca, Luciano Trozzi, Erica Novo, Nadia Panera, Cristiano De Stefanis, Valentina D'Oria, Marco Marzioni, Luca Maroni, Maurizio Parola, Anna Alisi, Gianluca Svegliati-Baroni
2344 related Products with: HDL cholesterol protects from liver injury in mice with intestinal specific LXRα activation.4 Arrays/Slide2 Pieces/Box100ug Lyophilized4 Membranes/Box2 Pieces/Box2 mg100ug4 Membranes/Box4 Arrays/Slide
#33098722 2020/10/24 To Up
Vitamin D decreases Porphyromonas gingivalis internalized into macrophages by promoting autophagy.This paper aims to study the effect of the active form of vitamin D (calcitriol) on the internalized P. gingivalis in macrophages and to assess the role of autophagy during this process.
Li Niu, Shuangshuang Chen, Xue Yang, Chunliang Ma, Chunling Pan, Hongyan Wang, Qian Li, Fengxue Geng, Xiaolin Tang
1842 related Products with: Vitamin D decreases Porphyromonas gingivalis internalized into macrophages by promoting autophagy.100 250ul1 mg200 assays200ul2.5 g100ug Lyophilized100ug Lyophilized 25 G1L100ug1 g
#33098689 2020/10/24 To Up
A mini-Tn5-derived transposon with reportable and selectable markers enables rapid generation and screening of insertional mutants in Gram-negative bacteria.We re-engineered a classic tool for mutagenesis and gene expression studies in Gram-negative bacteria. Our modified Tn5-based transposon contains multiple features that allow rapid selection for mutants, direct quantification of gene expression, and straightforward cloning of the inactivated gene. The promoter-less gfp-km cassette provides selection and reporter assay depending on the activity of the promoter upstream of the transposon insertion site. The cat gene facilitates positive antibiotic selection for mutants, while the narrow R6Kγ replication origin forces transposition in recipient strains lacking the pir gene and enables cloning the transposon flanked with the disrupted gene from the chromosome. The suicide vector pCKD100, a plasmid that could be delivered into recipient cells through bi-parental mating or electroporation, harbors the modified transposon. We used the transposon to mutagenize Pectobacterium versatile KD100, Pseudumonas coronafaciens PC27R, and Escherichia coli 35150N. The fluorescence intensities of mutants expressing high GFP could be quantified and detected qualitatively. Transformation efficiency from conjugation ranged from 1600 to 1900 CFU ml . We sequenced the upstream flanking regions, identified the putative truncated genes, and demonstrated the restoration of the GFP phenotype through marker exchange. The mini-Tn5 transposon was also utilized to construct mutant library of P. versatile for forward genetic screens.
Eric S Nazareno, Bimala Acharya, C Korsi Dumenyo
1950 related Products with: A mini-Tn5-derived transposon with reportable and selectable markers enables rapid generation and screening of insertional mutants in Gram-negative bacteria.1 mL400Tests25 mg100ug96T1 ml50 mg100 assays1000 tests
#33098674 2020/10/24 To Up
The Impact of Vitamin D Status on Cardiometabolic Effects of Fenofibrate in Women with Atherogenic Dyslipidemia.Vitamin D deficiency is a risk factor for cardiometabolic disease. The aim of this study was to determine the role of vitamin D status in the impact of fenofibrate on plasma levels of cardiometabolic risk factors.The study population (n=61) consisted of threematched groups of women with atherogenic dyslipidemia: vitamin D-naïve women with vitamin D insufficiency (group A), women receiving vitamin D preparations because of vitamin D deficiency (group B), as well as vitamin D-naïve women with normal vitamin D status (group C), who weretreated with micronized fenofibrate (200 mg daily).Glucose homeostasis markers, plasma lipids, as well as plasma levels of 25-hydroxyvitamin D, uric acid, high-sensitivity C-reactive protein (hsCRP), fibrinogen and homocysteine were determined at the beginning of the study and six months later.At entry, group A was characterized by lower levels of 25-hydroxyvitamin D, reduced insulin-sensitivity and higher concentrations of uric acid, hsCRP, fibrinogen and homocysteine. Apart from a weaker effect on HDL-cholesterol and triglycerides in group A, there were no differences between the treatment arms in the effect of fenofibrate on plasma lipids. However, only in groups B and C the drug improved insulin sensitivity and reduced circulating levels of uric acid andhsCRP, as well as increased levels of 25-hydroxyvitamin D and these effects correlated with the degree of improvement in insulin sensitivity. Treatment-induced increase in homocysteine was observed only in group A.The results of the study indicate that cardiometabolic effects of fibrates may depend on the vitamin D status of patients.
Robert Krysiak, Karolina Kowalcze, Bogusław Okopień
2199 related Products with: The Impact of Vitamin D Status on Cardiometabolic Effects of Fenofibrate in Women with Atherogenic Dyslipidemia.20 100 assays100ug
#33098662 2020/10/24 To Up
Using RAD-seq to develop sex-linked markers in a haplodiplontic alga.For many taxa, including isomorphic haplodiplontic macroalgae, determining sex and ploidy is challenging, thereby limiting the scope of some population demographic and genetic studies. Here, we used double digest restriction site-associated DNA sequencing (ddRAD-seq) to identify sex-linked molecular markers in the widespread red alga Agarophyton vermiculophyllum. In the ddRAD-seq library, we included 10 female gametophytes, 10 male gametophytes, and 16 tetrasporophytes from one native and one non-native site (N=40 gametophytes and N=32 tetrasporophytes total). We identified seven putatively female-linked and 19 putatively male-linked sequences. Four female- and eight male-linked markers amplified in all three life cycle stages. Using one female- and one male-linked marker that were sex-specific, we developed a duplex PCR and tested the efficacy of this assay on a subset of thalli sampled at two sites in the non-native range. We confirmed ploidy based on the visual observation of reproductive structures and previous microsatellite genotyping at 10 polymorphic loci. For 32 vegetative thalli, we were able to assign sex and confirm ploidy in these previously genotyped thalli. These markers will be integral to on-going studies of A. vermiculophyllum invasion. We discuss the utility of RAD-seq over other approaches previously used, such as RAPDs (random amplified polymorphic DNA), for future work designing sex-linked markers in other haplodiplontic macroalgae for which genomes are lacking.
Stacy A Krueger-Hadfield, Ben A Flanagan, Olivier Godfroy, Kristina M Hill-Spanik, Chris C Nice, Courtney J Murren, Allan E Strand, Erik E Sotka1 kit100 ug1 kit300 units0.1ml (1mg/ml)
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