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#34525437   2021/09/07 To Up

Ultrasound and heat treatment effects on Staphylococcus aureus cell viability in orange juice.

Ultrasounds are being considered an excellent alternative technology in juice preservation. Yet, when combined with heat treatment, the process seems to be further intensified. This work aimed to evaluate and compare the impact of ultrasounds and heat treatments, when applied alone or in combination, on Staphylococcus aureus survival in orange juice. Inoculated commercial pasteurized orange juice was treated at different times at 20, 30, 40, 50 and 60 °C. SEM analyses were applied to identify morphological changes in S. aureus cells appearance. The microbial inactivation data were fitted using two mathematical models, depending on the behaviour observed. Sonication at 20, 30, and 40 °C induced 4.02 ± 0.52, 3.80 ± 0.49 and 4.30 ± 0.74 log cycles reduction of S. aureus after treatments of 90, 60 and 60 min, respectively. The heat treatments at the same temperatures had no impact on S. aureus survival. When 50 and 60 °C were applied, more than 5-log reductions were attained for both thermosonication and heat treatments alone. A synergistic effect was observed between sonication and high temperatures. At 50 °C, the thermosonication reduced the treatment time from 60 to 35 min and the microbial load from 5.14 ± 0.08 to 10.76 ± 0.28 log cycles reduction, compared to heat treatment alone. Results from SEM images showed that cells undergo membrane damage during sonication exposure. This was observed by collapsed cells, cell disruption, and holes in the cell's membrane. Thermosonication proved to be a viable alternative to thermal pasteurization of orange juice since milder treatments can be safely applied, improving the final product quality.
Akila Amir Tahi, Sérgio Sousa, Khodir Madani, Cristina L M Silva, Fátima A Miller

1706 related Products with: Ultrasound and heat treatment effects on Staphylococcus aureus cell viability in orange juice.

1 kit5000100 µg1x10e7 cells-100ug Lyophilized1 kit50 ml100 μg200

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#34525402   2021/09/12 To Up

The cathelicidin-derived close-to-nature peptide D-11 sensitizes Klebsiella pneumoniae to a range of antibiotics in vitro, ex vivo and in vivo.

The outer membrane of Gram-negative bacteria constitutes a permeability barrier that prevents certain antibiotics to reach their targets, thus conferring a high tolerance to a wide range of antibiotics. Combined therapies of antibiotics and outer membrane-perturbing drugs have been proposed as an alternative treatment to extend the use of antibiotics against Gram-positive bacteria to Gram-negative bacteria. Among the outer-membrane active compounds, the outer-membrane permeabilizing peptides, play a prominent role. They form a group of small cationic and amphipathic molecules with the ability to insert specifically into bacterial membranes inducing their permeabilization and/or disruption. Here we assess the combined effect of several compounds belonging to main antibiotic families and the cathelicidin close-to-nature outer membrane peptide D-11 against four clinically relevant Gram-negative bacteria. The results show that the peptide D-11 displays strong synergistic activity with several antibiotics belonging to different families, in particular against Klebsiella pneumoniae, even better than some other outer membrane-active peptides that are currently in a clinical trial such as SPR741. Notably, we observed this activity in vitro, ex vivo in a newly designed bacteremia model and in vivo in a mice abscess infection model. Overall, our results suggest that D-11 is a good candidate to repurpose the activity of traditional antibiotics against Klebsiella pneumoniae.
Rubén Cebrián, Congjuan Xu, Yushan Xia, Weihui Wu, Oscar P Kuipers

2446 related Products with: The cathelicidin-derived close-to-nature peptide D-11 sensitizes Klebsiella pneumoniae to a range of antibiotics in vitro, ex vivo and in vivo.

96 tests48 samples25 1 mg1.00 flask

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#34525378   2021/09/01 To Up

Antihypertensive drug treatment and susceptibility to SARS-CoV-2 infection in human PSC-derived cardiomyocytes and primary endothelial cells.

The pathogenicity of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) has been attributed to its ability to enter through the membrane-bound angiotensin-converting enzyme 2 (ACE2) receptor. Therefore, it has been heavily speculated that angiotensin-converting enzyme inhibitor (ACEI) or angiotensin receptor blocker (ARB) therapy may modulate SARS-CoV-2 infection. In this study, exposure of human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) and human endothelial cells (hECs) to SARS-CoV-2 identified significant differences in protein coding genes involved in immunity, viral response, and cardiomyocyte/endothelial structure. Specifically, transcriptome changes were identified in the tumor necrosis factor (TNF), interferon α/β, and mitogen-activated protein kinase (MAPK) (hPSC-CMs) as well as nuclear factor kappa-B (NF-κB) (hECs) signaling pathways. However, pre-treatment of hPSC-CMs or hECs with two widely prescribed antihypertensive medications, losartan and lisinopril, did not affect the susceptibility of either cell type to SARS-CoV-2 infection. These findings demonstrate the toxic effects of SARS-CoV-2 in hPSC-CMs/hECs and, taken together with newly emerging multicenter trials, suggest that antihypertensive drug treatment alone does not alter SARS-CoV-2 infection.
Jessika Iwanski, Sobhi G Kazmouz, Shuaizhi Li, Ben Stansfield, Tori T Salem, Samantha Perez-Miller, Toshinobu Kazui, Lipsa Jena, Jennifer L Uhrlaub, Scott Lick, Janko Nikolich-Žugich, John P Konhilas, Carol C Gregorio, May Khanna, Samuel K Campos, Jared M Churko

2111 related Products with: Antihypertensive drug treatment and susceptibility to SARS-CoV-2 infection in human PSC-derived cardiomyocytes and primary endothelial cells.

1.00 flask1.00 flask1.00 flask1.00 flask1.00 flask1.00 flask100ug Lyophilized1.00 flask100ug Lyophilized1.00 flask1.00 flask

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#34525362   // To Up

ER exit sites in Drosophila display abundant ER-Golgi vesicles and pearled tubes but no megacarriers.

Secretory cargos are collected at endoplasmic reticulum (ER) exit sites (ERES) before transport to the Golgi apparatus. Decades of research have provided many details of the molecular events underlying ER-Golgi exchanges. Essential questions, however, remain about the organization of the ER-Golgi interface in cells and the type of membrane structures mediating traffic from ERES. To investigate these, we use transgenic tagging in Drosophila flies, 3D-structured illumination microscopy (SIM), and focused ion beam scanning electron microscopy (FIB-SEM) to characterize ERES-Golgi units in collagen-producing fat body, imaginal discs, and imaginal discs overexpressing ERES determinant Tango1. Facing ERES, we find a pre-cis-Golgi region, equivalent to the vertebrate ER-Golgi intermediate compartment (ERGIC), involved in both anterograde and retrograde transport. This pre-cis-Golgi is continuous with the rest of the Golgi, not a separate compartment or collection of large carriers, for which we find no evidence. We observe, however, many vesicles, as well as pearled tubules connecting ERES and Golgi.
Ke Yang, Min Liu, Zhi Feng, Marta Rojas, Lingjian Zhou, Hongmei Ke, José Carlos Pastor-Pareja

1178 related Products with: ER exit sites in Drosophila display abundant ER-Golgi vesicles and pearled tubes but no megacarriers.

50 mg

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#34525347   2021/09/09 To Up

Harnessing features of adaptive NK cells to generate iPSC-derived NK cells for enhanced immunotherapy.

Select subsets of immune effector cells have the greatest propensity to mediate antitumor responses. However, procuring these subsets is challenging, and cell-based immunotherapy is hampered by limited effector-cell persistence and lack of on-demand availability. To address these limitations, we generated a triple-gene-edited induced pluripotent stem cell (iPSC). The clonal iPSC line was engineered to express a high affinity, non-cleavable version of the Fc receptor CD16a and a membrane-bound interleukin (IL)-15/IL-15R fusion protein. The third edit was a knockout of the ecto-enzyme CD38, which hydrolyzes NAD. Natural killer (NK) cells derived from these uniformly engineered iPSCs, termed iADAPT, displayed metabolic features and gene expression profiles mirroring those of cytomegalovirus-induced adaptive NK cells. iADAPT NK cells persisted in vivo in the absence of exogenous cytokine and elicited superior antitumor activity. Our findings suggest that unique subsets of the immune system can be modeled through iPSC technology for effective treatment of patients with advanced cancer.
Karrune V Woan, Hansol Kim, Ryan Bjordahl, Zachary B Davis, Svetlana Gaidarova, John Goulding, Brian Hancock, Sajid Mahmood, Ramzey Abujarour, Hongbo Wang, Katie Tuininga, Bin Zhang, Cheng-Ying Wu, Behiye Kodal, Melissa Khaw, Laura Bendzick, Paul Rogers, Moyar Qing Ge, Greg Bonello, Miguel Meza, Martin Felices, Janel Huffman, Thomas Dailey, Tom T Lee, Bruce Walcheck, Karl J Malmberg, Bruce R Blazar, Yenan T Bryceson, Bahram Valamehr, Jeffrey S Miller, Frank Cichocki

2769 related Products with: Harnessing features of adaptive NK cells to generate iPSC-derived NK cells for enhanced immunotherapy.

1.00 flask4 x 96-well plate1.00 flask100 extractions1.00 flask1x10e7 cells10100ml 1 kit(s) 0.5 ml 100ul100ul

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#34525342   2021/09/11 To Up

A PtdIns(3,4,5)P dispersal switch engages cell ratcheting at specific cell surfaces.

Force generation in epithelial tissues is often pulsatile, with actomyosin networks generating contractile forces before cyclically disassembling. This pulsed nature of cytoskeletal forces implies that there must be ratcheting mechanisms that drive processive transformations in cell shape. Previous work has shown that force generation is coordinated with endocytic remodeling; however, how ratcheting becomes engaged at specific cell surfaces remains unclear. Here, we report that PtdIns(3,4,5)P is a critical lipid-based cue for ratcheting engagement. The Sbf RabGEF binds to PIP, and disruption of PIP reveals a dramatic switching behavior in which medial ratcheting is activated and epithelial cells begin globally constricting apical surfaces. PIP enrichments are developmentally regulated, with mesodermal cells having high apical PIP while germband cells have higher interfacial PIP. Finally, we show that JAK/STAT signaling constitutes a second pathway that combinatorially regulates Sbf/Rab35 recruitment. Our results elucidate a complex lipid-dependent regulatory machinery that directs ratcheting engagement in epithelial tissues.
Hui Miao, Timothy E Vanderleest, Rashmi Budhathoki, Dinah Loerke, J Todd Blankenship

1106 related Products with: A PtdIns(3,4,5)P dispersal switch engages cell ratcheting at specific cell surfaces.

2 Pieces/Box2 Pieces/Box2 Pieces/Box2 Pieces/Box100ug Lyophilized100ug Lyophilized1 kit1 kit

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#34525326   2021/09/08 To Up

Rieske head domain dynamics and indazole-derivative inhibition of Candida albicans complex III.

Electron transfer between respiratory complexes drives transmembrane proton translocation, which powers ATP synthesis and membrane transport. The homodimeric respiratory complex III (CIII) oxidizes ubiquinol to ubiquinone, transferring electrons to cytochrome c and translocating protons through a mechanism known as the Q cycle. The Q cycle involves ubiquinol oxidation and ubiquinone reduction at two different sites within each CIII monomer, as well as movement of the head domain of the Rieske subunit. We determined structures of Candida albicans CIII by cryoelectron microscopy (cryo-EM), revealing endogenous ubiquinone and visualizing the continuum of Rieske head domain conformations. Analysis of these conformations does not indicate cooperativity in the Rieske head domain position or ligand binding in the two CIIIs of the CIII dimer. Cryo-EM with the indazole derivative Inz-5, which inhibits fungal CIII and is fungicidal when administered with fungistatic azole drugs, showed that Inz-5 inhibition alters the equilibrium of Rieske head domain positions.
Justin M Di Trani, Zhongle Liu, Luke Whitesell, Peter Brzezinski, Leah E Cowen, John L Rubinstein

1292 related Products with: Rieske head domain dynamics and indazole-derivative inhibition of Candida albicans complex III.

100 ug1 mL1 mL1 mg1 mL100ug10 mgEach1 kit(96 Wells)5mg100ug250ul

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#34525325   2021/09/15 To Up

On the electrical passivity of astrocyte potassium conductance.

Predominant expression of leak-type K channels provides astrocytes a high membrane permeability to K ions and a hyperpolarized membrane potential that are crucial for astrocyte function in brain homeostasis. In functionally mature astrocytes, the expression of leak K channels creates a unique membrane K conductance that lacks voltage-dependent rectification. Accordingly, the conductance is named ohmic or passive K conductance. Several inwardly rectifiers and two-pore domain K channels have been investigated for their contributions to passive conductance. Meanwhile, gap junctional coupling has been postulated to underlie the passive behavior of membrane conductance. It is now clear that the intrinsic properties of K channels and gap junctional coupling can each act alone or together to bring about a passive behavior of astrocyte conductance. Additionally, while the passive conductance can generally be viewed as a K conductance, the actual representation of this conductance is a combined expression of multiple known and unknown K channels, which has been further modified by the intricate morphology of individual astrocytes and syncytial gap junctional coupling. The expression of the inwardly rectifying K channels explains the inward-going component of passive conductance disobeying Goldman-Hodgkin-Kate (GHK) constant field outward rectification. However, the K channels encoding the outward-going passive currents remain to be determined in the future. Here, we review our current understanding of ion channels and biophysical mechanisms engaged in the passive astrocyte K conductance, propose new studies to resolve this long-standing puzzle in astrocyte physiology, and discuss the functional implication(s) of passive behavior of K conductance on astrocyte physiology.
Min Zhou, Yixing Du, Sydney Aten, David Terman

1591 related Products with: On the electrical passivity of astrocyte potassium conductance.

1KG100ug Lyophilized 500 ml 50 IU 2 ml Ready-to-use 1 G10x50 ug100.00 ul2.5 mg 500 G10 mg

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#34525323   2021/09/15 To Up

THE PLASTICITY OF NERVE FIBRES: THE PROLONGED EFFECTS OF POLARIZATION OF AFFERENT FIBRES.

The review surveys various aspects of the plasticity of nerve fibres, in particular the prolonged increase in their excitability evoked by polarization, focusing on a long-lasting increase in the excitability of myelinated afferent fibres traversing the dorsal columns of the spinal cord. We review the evidence that increased axonal excitability (i) follows epidurally applied direct current as well as relatively short (5 or 10 ms) current pulses and synaptically evoked intrinsic field potentials; (ii) critically depends on the polarization of branching regions of afferent fibres at the sites where they bifurcate and give off axon collaterals entering the spinal grey matter in conjunction with actions of extrasynaptic GABA membrane receptors; and (iii) shares the feature of being activity-independent with the short-lasting effects of polarization of peripheral nerve fibres. A comparison between the polarization evoked sustained increase in the excitability of dorsal column fibres and spinal motoneurons (plateau potentials) indicates the possibility that they are mediated by partly similar membrane channels (including non-inactivating type L Cav 1.3 but not Na+ channels) and partly different mechanisms. We finally consider under which conditions trans-spinally applied DC (tsDCS) might reproduce the effects of epidural polarization on dorsal column fibres and the possible advantages of increased excitability of afferent fibres for the rehabilitation of motor and sensory functions after spinal cord injuries.
Elzbieta Jankowska, Ingela Hammar

2050 related Products with: THE PLASTICITY OF NERVE FIBRES: THE PROLONGED EFFECTS OF POLARIZATION OF AFFERENT FIBRES.

5 G200 units100 1500IUmin 2 cartons100.00 ul200 units

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#34525277   2021/09/15 To Up

Safety and Efficacy of the BNT162b2 mRNA Covid-19 Vaccine through 6 Months.

BNT162b2 is a lipid nanoparticle-formulated, nucleoside-modified RNA vaccine encoding a prefusion-stabilized, membrane-anchored severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) full-length spike protein. BNT162b2 is highly efficacious against coronavirus disease 2019 (Covid-19) and is currently approved, conditionally approved, or authorized for emergency use worldwide. At the time of initial authorization, data beyond 2 months after vaccination were unavailable.
Stephen J Thomas, Edson D Moreira, Nicholas Kitchin, Judith Absalon, Alejandra Gurtman, Stephen Lockhart, John L Perez, Gonzalo Pérez Marc, Fernando P Polack, Cristiano Zerbini, Ruth Bailey, Kena A Swanson, Xia Xu, Satrajit Roychoudhury, Kenneth Koury, Salim Bouguermouh, Warren V Kalina, David Cooper, Robert W Frenck, Laura L Hammitt, Özlem Türeci, Haylene Nell, Axel Schaefer, Serhat Ünal, Qi Yang, Paul Liberator, Dina B Tresnan, Susan Mather, Philip R Dormitzer, Uğur Şahin, William C Gruber, Kathrin U Jansen,

1893 related Products with: Safety and Efficacy of the BNT162b2 mRNA Covid-19 Vaccine through 6 Months.

100 mg25 mg25 mg1000 TESTS/0.65ml25 g 1KG100ug Lyophilized 5 G10 mg1000 tests 1 G96 wells (1 kit)

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