Search results for: Mouse Anti-Feline CD8 alpha
#9613469 // To Up
Characterization of anti-feline CD8 monoclonal antibodies.
We generated three monoclonal antibodies (mAbs) (2D7, 10C7 and 12A3) reactive to the alpha-chain of feline CD8 (fCD8) molecule. Further we showed that reference anti-fCD8 mAbs, FT2, 3.357 and vpg9 recognize the beta-chain, alpha-chain and alphabeta-complex epitope, respectively. Flow cytometric analysis using these mAbs suggested that fCD8alpha(+)beta(-) cells were present in lymphocytes of spleen, but not significantly in those of thymus, lymph nodes and peripheral blood of normal kittens.M Shimojima, M R Pecoraro, K Maeda, Y Tohya, T Miyazawa, T Mikami
1267 related Products with: Characterization of anti-feline CD8 monoclonal antibodies.
100.00 ug100.00 ug100.00 ug0.1 mg100.00 ug100.00 ug100.00 ug100.00 ug100.00 ug100.00 ug100.00 ug100.00 ugRelated Pathways
#8132208 // To Up
Isolation, sequence and expression of a cDNA encoding the alpha-chain of the feline CD8.
We have cloned, sequenced and expressed a cDNA encoding the alpha-chain of feline CD8. This clone, named FT8-10, has an open reading frame with 720 nucleotides in length encoding a protein with 239 amino acid residues. Sequence analysis has revealed that the feline CD8 alpha-chain (CD8 alpha) shares significant homology with human (T8/Leu-2), bovine (BoCD8), rat (MRC OX8) and mouse (Lyt-2) CD8 alpha subunits. Cysteine residues as well as the tyrosine kinase p56lck binding site are well conserved. Besides, no putative N-linked glycosylation site was found. Interestingly, immunofluorescence analysis of COS-7 cells transfected with feline CD8 alpha expression plasmid driven by SR alpha promoter showed that the expressed feline CD8 alpha cross-reacted with an anti-human CD8 alpha monoclonal antibody OKT8, but did not react with an anti-feline monoclonal antibody, FT-2, which is thought to recognize the feline analogue of the human T8/Leu-2 and murine Lyt-2 molecules expressed on cytotoxic/suppressor T cells.M R Pecoraro, Y Kawaguchi, T Miyazawa, J Norimine, K Maeda, T Toyosaki, Y Tohya, C Kai, T Mikami
1086 related Products with: Isolation, sequence and expression of a cDNA encoding the alpha-chain of the feline CD8.
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