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Search results for: Mouse Anti-Pig Endothelial Cells Antibodies

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#31464569   2019/08/29 To Up

Epitope Mapping of Antipig Podoplanin Monoclonal Antibody PMab-213.

Podoplanin (PDPN), a type I transmembrane sialoglycoprotein, is expressed on lymphatic endothelial cells, podocytes of the kidneys, and type I alveolar cells of the lungs. PDPN, a platelet aggregation-inducing factor, comprises three platelet aggregation-stimulating (PLAG) domains (PLAG1, PLAG2, and PLAG3) in the N-terminus and PLAG-like domains in the middle of the PDPN protein. We have previously reported a mouse antipig PDPN (pPDPN) monoclonal antibody (mAb) clone, PMab-213, which was developed using the Cell-Based Immunization and Screening (CBIS) method. PMab-213 is very useful in flow cytometry, Western blotting, and immunohistochemical analyses; however, the binding epitope of PMab-213, which was developed by CBIS method, remains to be elucidated. Therefore, this study aimed to investigate the epitope of PMab-213 using enzyme-linked immunosorbent assay, flow cytometry, and immunohistochemical analyses. The results revealed that the critical epitopes of PMab-213 are Pro53, Arg54, Arg56, and Tyr60 of pPDPN.
Shinji Yamada, Shunsuke Itai, Yoshikazu Furusawa, Mika K Kaneko, Yukinari Kato

2581 related Products with: Epitope Mapping of Antipig Podoplanin Monoclonal Antibody PMab-213.

100ul100ug Lyophilized100 μg0.1 mg100 ul100 ug1 mg100ul100ug100 ug100 ul100μg

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#30681406   2019/01/25 To Up

PMab-210: A Monoclonal Antibody Against Pig Podoplanin.

Podoplanin (PDPN) is a type I transmembrane glycoprotein that is expressed in normal tissues, including renal corpuscles and type I lung alveolar cells. Monoclonal antibodies (mAbs) against human, mouse, rat, rabbit, dog, cat, and bovine PDPNs have already been established; however, antipig PDPN (pPDPN) mAbs have not. We therefore immunized mice with pPDPN-overexpressing Chinese hamster ovary (CHO)-K1 cells (CHO/pPDPN), and screened hybridomas, which are producing anti-pPDPN mAbs. One of mAbs, PMab-210 (an IgG, kappa), was able to specifically detect CHO/pPDPN cells by flow cytometry and detect pPDPN by Western blot analysis. Furthermore, PMab-210 strongly stained type I lung alveolar cells and weakly stained renal corpuscles by immunohistochemistry. PMab-210 is expected to be useful in investigating the function of pPDPN.
Yoshikazu Furusawa, Shinji Yamada, Shunsuke Itai, Masato Sano, Takuro Nakamura, Miyuki Yanaka, Masato Fukui, Hiroyuki Harada, Takuya Mizuno, Yusuke Sakai, Masaki Takasu, Mika K Kaneko, Yukinari Kato

2942 related Products with: PMab-210: A Monoclonal Antibody Against Pig Podoplanin.

100 μg100ug0.1 mg 50UG100ul50 µg 100 ug25 µg1 mg100 ug100ug100ul

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