Search results for: Myeloperoxidase (MPO) Colorimetric Activity Assay Kit
#36405709 
2022/11/02
To Up
Sex-related differences in the response of anti-platelet drug therapies targeting purinergic signaling pathways in sepsis.
Sepsis, a complex clinical syndrome resulting from a serious infection, is a major healthcare problem associated with high mortality. Sex-related differences in the immune response to sepsis have been proposed but the mechanism is still unknown. Purinergic signaling is a sex-specific regulatory mechanism in immune cell physiology. Our studies have shown that blocking the ADP-receptor P2Y but not P2Y receptor was protective in male mice during sepsis, but not female. We now hypothesize that there are sex-related differences in modulating P2Y or P2Y signaling pathways during sepsis. Male and female wild-type (WT), P2Y knock-out (KO), and P2Y KO mice underwent sham surgery or cecal ligation and puncture (CLP) to induce sepsis. The P2Y antagonist ticagrelor or the P2Y antagonist MRS2279 were administered intra-peritoneally after surgery to septic male and female mice. Blood, lungs and kidneys were collected 24 hours post-surgery. Sepsis-induced changes in platelet activation, secretion and platelet interaction with immune cells were measured by flow cytometry. Neutrophil infiltration in the lung and kidney was determined by a myeloperoxidase (MPO) colorimetric assay kit. Sepsis-induced platelet activation, secretion and aggregate formation were reduced in male CLP P2Y KO and in female CLP P2Y KO mice compared with their CLP WT counterpart. Sepsis-induced MPO activity was reduced in male CLP P2Y KO and CLP P2Y KO female mice. CLP males treated with ticagrelor or MRS2279 showed a decrease in sepsis-induced MPO levels in lung and kidneys, aggregate formation, and platelet activation as compared to untreated male CLP mice. There were no differences in platelet activation, aggregate formation, and neutrophil infiltration in lung and kidney between female CLP mice and female CLP mice treated with ticagrelor or MRS2279. In human T lymphocytes, blocking P2Y or P2Y alters cell growth and secretion in a sex-dependent manner, supporting the data obtained in mice. In conclusion, targeting purinergic signaling represents a promising therapy for sepsis but drug targeting purinergic signaling is sex-specific and needs to be investigated to determine sex-related targeted therapies in sepsis.Emmanuel Boadi Amoafo, Philomena Entsie, Samara Albayati, Glenn P Dorsam, Satya P Kunapuli, Laurie E Kilpatrick, Elisabetta Liverani
1143 related Products with: Sex-related differences in the response of anti-platelet drug therapies targeting purinergic signaling pathways in sepsis.
100 UG2 Pieces/Box100 μg100 μg100 μg100 μg5 100 μg100 μg
Related Pathways
#32460745 2020/05/27
To Up
Shen-ling-bai-zhu-san ameliorates inflammation and lung injury by increasing the gut microbiota in the murine model of Streptococcus pneumonia-induced pneumonia.
Shen-ling-bai-zhu-san (SLBZS) regulates inflammation and gut microbiota which are associated with Streptococcus pneumoniae (Spn)-induced pneumonia. So, we studied the therapeutic effect of SLBZS and evaluated whether gut microbiota is associated with the effects of SLBZS in improving Spn-induced pneumonia.Jinli Feng, Weibo Dai, Cheng Zhang, Houjun Chen, Ziliang Chen, Yongfeng Chen, Qianyi Pan, Yongmao Zhou
1596 related Products with: Shen-ling-bai-zhu-san ameliorates inflammation and lung injury by increasing the gut microbiota in the murine model of Streptococcus pneumonia-induced pneumonia.
14 Sample Kit500 Units
Related Pathways
#31815144 2019/11/13
To Up
Mitochondrial Coenzyme Q Protects Sepsis-Induced Acute Lung Injury by Activating PI3K/Akt/GSK-3/mTOR Pathway in Rats.
The aim of our study was to assess the effects of mitochondrial coenzyme Q (MitoQ) on sepsis-induced acute lung injury (ALI) and investigate its possible mechanisms. The cecal ligation and puncture (CLP) method was used to establish a septic ALI model. Rats were randomly divided into Con group, CLP group, MitoQ group, and MitoQ + LY294002 group. The survival rate of the rats was recorded, and the survival rate curve was plotted. Moreover, the ratio of wet/dry weight (W/D) in lung tissue was measured. The activity of myeloperoxidase (MPO) was measured by using the MPO colorimetric activity assay kit. The levels of high-mobility group box 1 (HMGB1) and interleukin-6 (IL-6), macrophage inflammatory protein 2 (MIP2), and keratinocyte chemoattractant (KC) were analyzed by ELISA. The histopathological changes were measured by HE staining, and the lung injury was scored. TUNEL assay was applied to detect the apoptotic cells in lung tissue. The protein expressions were detected by western blot. MitoQ increased the survival rate and alleviated pulmonary edema in septic ALI rats. In addition, MitoQ inhibited the MPO activity and decreased the levels of HMGB1 and IL-6. After treatment with MitoQ, alveolar wall edema, inflammatory cell infiltration, and red blood cell exudation were relieved. MitoQ inhibited cell apoptosis in lung tissue of septic ALI rats. Meanwhile, MitoQ treatment remarkedly increased the expression of p-Akt, p-GSK-3, and p-mTOR but decreased Bax, caspase-3, caspase-9, Beclin-1, and LC-3II/LC-3I. The effects of MitoQ were significantly reversed by the PI3K inhibitor (LY294002). Our study demonstrated that MitoQ could protect sepsis-induced acute lung injury by activating the PI3K/Akt/GSK-3/mTOR pathway in rats.Ruirui Li, Tao Ren, Jianqiong Zeng