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Search results for: Myoglobin

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#33624548   2021/02/24 To Up

Time course of changes in V̇O and O extraction during ramp cycle exercise following HIIT vs moderate-intensity continuous training in type 2 diabetes.

In the present study we assessed the time course of adaptations in peak oxygen uptake (V̇O) and muscle fractional oxygen (O) extraction (using near-infrared spectroscopy) following 12 weeks of low-volume high-intensity interval training (HIIT) vs. moderate-intensity continuous endurance training (MICT) in adults with uncomplicated type 2 diabetes (T2D). Participants with T2D were randomly assigned to MICT (n = 12, 50 min of moderate-intensity cycling), HIIT (n = 9, 10 x 1 min at ~90% maximal heart rate) or to a non-exercising control group (n = 9). Exercising groups trained 3 times per week and measurements were taken every 3 weeks. The rate of muscle deoxygenation (i.e. deoxygenated haemoglobin and myoglobin concentration, Δ[HHb+Mb]) profiles of the vastus lateralis muscle were normalised to 100% of the response, plotted against % power output (PO) and fitted with a double linear regression model. V̇O increased (P<0.05) by week 3 of MICT (+17%) and HIIT (+8%), with no further significant changes thereafter. Total increases in V̇O posttraining (P<0.05) were 27% and 14% respectively. The %∆[HHb+Mb] vs %PO slope of the first linear segment (slope) was reduced (P<0.05) beyond 3 weeks of HIIT and MICT with no further significant changes thereafter. No changes in V̇O or slope were observed in the control group. Low-volume HIIT and MICT induced improvements in V̇O following a similar time course and these improvements were likely, at least in part, due to an improved microvascular O delivery.
Norita Gildea, Adam McDermott, Joel Rocha, Donal O'Shea, Simon Green, Mikel Egaña

1706 related Products with: Time course of changes in V̇O and O extraction during ramp cycle exercise following HIIT vs moderate-intensity continuous training in type 2 diabetes.

110mg10 mg 25 G

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#33621829   2021/02/13 To Up

Changes in color, myoglobin, and lipid oxidation in beef patties treated by dielectric barrier discharge cold plasma during storage.

The objective of this work was to investigate the changes in color, metmyoglobin reducing activity (MRA), and lipid oxidation (TBARS) and their relationships in beef patties treated by dielectric barrier discharge cold plasma (DBD-CP) at different voltages and frequencies during storage at 4 °C. DBD-CP treatment, including treatment voltages and frequencies, caused the decrease of a value and MRA (P < 0.05), promoted the accumulation of metmyoglobin (P < 0.05), and significantly increased TBARS values (P < 0.05). It was the first time revealed that the effect of treatment voltage on the quality of beef patty was greater than that of frequency. In addition, there were significant and strong positive correlations between a value and MRA and negative correlations between a value, metmyoglobin (MMb)% and TBARS. Taken together, Data demonstrated that DBD-CP treatment can significantly affect meat redness, Mb contents, MRA, and lipid oxidation in ground beef during storage, and accelerate discoloration and lipid oxidation of meat.
Xiaoting Wang, Zhaobin Wang, Hong Zhuang, Mustapha Muhammad Nasiru, Yuan Yuan, Jianhao Zhang, Wenjing Yan

2514 related Products with: Changes in color, myoglobin, and lipid oxidation in beef patties treated by dielectric barrier discharge cold plasma during storage.

0.05 mg10 rxns

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#33616701   2021/02/22 To Up

[Musculoskeletal laboratory diagnostics in competitive sport].

Laboratory diagnostics represent a valuable tool for the optimization and assessment of the performance and regeneration ability in professional athletes. Blood parameters play an important role in the prevention, diagnosis and rehabilitation of injuries and physical overload.
Maximilian M Delsmann, Julian Stürznickel, Michael Amling, Peter Ueblacker, Tim Rolvien

1908 related Products with: [Musculoskeletal laboratory diagnostics in competitive sport].

96 tests1roll10mg100ug100 μg100ug100 μg0.1ml

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#33604206   2021/01/12 To Up

Rhabdomyolysis Causing Renal Failure Following Cardiopulmonary Resuscitation, Cardioversion, and Myocardial Infarction: A Case Report and Review of the Literature.

Rhabdomyolysis is a condition where there is damage of skeletal muscle, causing myoglobin leak into the circulation. We report a case of a 69-year-old female with a history of hypertension, hyperlipidemia, diabetes mellitus, morbid obesity, paroxysmal atrial fibrillation, and chronic kidney disease stage who underwent cardiopulmonary resuscitation following ventricular fibrillation to restore effective cardiac rhythm. After the third attempt of defibrillation she converted to sinus rhythm. Her echocardiography was suggestive of myocardial infarction (MI). On the second day of her hospitalization, she started becoming oliguric and her creatinine started rising up causing acute kidney injury (AKI). The patient's creatinine kinase (CK) level peaked at 6380 u/L (normal range 26-192 u/L), myoglobin was >20,000 ng/mL (normal range 9-83 ng/mL), and myocardial bound (MB) isoenzyme of CK was 4.5 ng/mL (normal range 0-3.6 ng/mL). Plasma creatinine increased to 5.71 mg/dL and ultimately developed renal failure. She was started on hemodialysis. Her cardiac catheterization was suggestive of MI. Our case highlights that MI, cardiopulmonary resuscitation, and cardioversion can be a cause for myoglobinuric renal failure, which has been rarely reported in the literature before.
Sonia Gupta, Vinay Kumar Thallapally, Joseph Thirumalareddy

1086 related Products with: Rhabdomyolysis Causing Renal Failure Following Cardiopulmonary Resuscitation, Cardioversion, and Myocardial Infarction: A Case Report and Review of the Literature.

1000 tests100ul1000 TESTS/0.65ml100ug100 mg10 mg100ul500 MG2.5 mg25 mg

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#33585426   2021/01/21 To Up

A Novel Design of Temporomandibular Joint Prosthesis for Lateral Pterygoid Muscle Attachment: A Preliminary Study.

In temporomandibular joint (TMJ) replacement operation, due to the condylectomy, the lateral pterygoid muscle (LPM) lost attachment and had impact on the mandible kinematic function. This study aimed to design a novel TMJ replacement prosthesis for LPM attachment and to verify its feasibility by preliminary and experiments. An artificial TMJ prosthesis designed with a porous structure on the condylar neck region for LPM attachment was fabricated by a 3D printed titanium (Ti) alloy. A rat myoblast cell line (L6) was tested for adhesion and biocompatibility with porous titanium scaffolds by cell counting Kit-8 (CCK-8), scanning electron microscope (SEM), flow cytometry (FCM), real-time quantitative polymerase chain reaction (RT-qPCR), immunocytofluorescense, western blotting, etc. The porous titanium scaffolds were further embedded in the rat intervertebral muscle to analyze muscle growth and biomechanical strength . The novel artificial TMJ prosthesis was implanted to reconstruct the goat's condyle and LPM reattachment was analyzed by hard tissue section and avulsion force test. L6 muscle cells showed good proliferation potential on the porous Ti scaffold under SEM scanning and FCM test. In RT-qPCR, immunocytofluorescense and western blotting tests, the L6 cell lines had good myogenic capacity when cultured on the scaffold with high expression of factors such as Myod1 and myoglobin, etc. In the experiment, muscles penetrated into the porous scaffold in both rats and goats. In rat's intervertebral muscle implantation, the avulsion force was 0.716 N/mm in 4 weeks after operation and was significantly increased to 0.801 N/mm at 8 weeks ( < 0.05). In goat condylar reconstruction with the porous scaffold prosthesis, muscles attached to the prosthesis with the avulsion force of 0.436 N/mm at 8 weeks, but was smaller than the biological muscle-bone attachment force. The novel designed TMJ prosthesis can help LPM attach to its porous titanium scaffold structure area for future function.
Luxiang Zou, Yingqian Zhong, Yinze Xiong, Dongmei He, Xiang Li, Chuan Lu, Huimin Zhu

1640 related Products with: A Novel Design of Temporomandibular Joint Prosthesis for Lateral Pterygoid Muscle Attachment: A Preliminary Study.

0.1 mg100 assays 6 ml Ready-to-use 100ul10 mg 100ul25 g100Tests200ul0.2 mg1 Kit10 nmol

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#33584997   2021/02/09 To Up

3044 Cases reveal important prognosis signatures of COVID-19 patients.

Critical patients and intensive care unit (ICU) patients are the main population of COVID-19 deaths. Therefore, establishing a reliable method is necessary for COVID-19 patients to distinguish patients who may have critical symptoms from other patients. In this retrospective study, we firstly evaluated the effects of 54 laboratory indicators on critical illness and death in 3044 COVID-19 patients from the Huoshenshan hospital in Wuhan, China. Secondly, we identify the eight most important prognostic indicators (neutrophil percentage, procalcitonin, neutrophil absolute value, C-reactive protein, albumin, interleukin-6, lymphocyte absolute value and myoglobin) by using the random forest algorithm, and find that dynamic changes of the eight prognostic indicators present significantly distinct within differently clinical severities. Thirdly, our study reveals that a model containing age and these eight prognostic indicators can accurately predict which patients may develop serious illness or death. Fourthly, our results demonstrate that different genders have different critical illness rates compared with different ages, in particular the mortality is more likely to be attributed to some key genes (e.g. ACE2, TMPRSS2 and FURIN) by combining the analysis of public lung single cells and bulk transcriptome data. Taken together, we urge that the prognostic model and first-hand clinical trial data generated in this study have important clinical practical significance for predicting and exploring the disease progression of COVID-19 patients.
Shijie Qin, Weiwei Li, Xuejia Shi, Yanjun Wu, Canbiao Wang, Jiawei Shen, Rongrong Pang, Bangshun He, Jun Zhao, Qinghua Qiao, Tao Luo, Yanju Guo, Yang Yang, Ying Han, Qiuyue Wu, Jian Wu, Wei Dai, Libo Zhang, Liming Chen, Chunyan Xue, Ping Jin, Zhenhua Gan, Fei Ma, Xinyi Xia

2650 related Products with: 3044 Cases reveal important prognosis signatures of COVID-19 patients.

1 mg

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#33578251   2021/01/28 To Up

Rational design of metal-binding sites in domain-swapped myoglobin dimers.

The metal active site is precisely designed in metalloproteins. Here we applied 3D domain swapping, a phenomenon in which a partial protein structure is exchanged between molecules, to introduce metal sites in proteins. We designed multiple metal-binding sites specific to domain-swapped myoglobin (Mb) with His mutation. Stable dimeric Mbs with metal-binding sites were obtained by shifting the His position and introducing two Ala residues in the hinge region (K78H/G80A/H82A and K79H/G80A/H81A Mbs). The absorption and circular dichroism spectra of the monomer and dimer of K78H/G80A/H82A and K79H/G80A/H81A Mbs were similar to the corresponding spectra, respectively, of wild-type Mb. No negative peak due to dimer-to-monomer dissociation was observed below the denaturation temperature in the differential scanning calorimetry thermograms of K78H/G80A/H82A and K79H/G80A/H81A Mbs, whereas the dimer dissociates into monomers at 68 °C for wild-type Mb. These results show that the two mutants were stable in the dimer state. Metal ions bound to the metal-binding sites containing the introduced His in the domain-swapped Mb dimers. Co-bound and Ni-bound K78H/G80A/H82A Mb exhibited octahedral metal-coordination structures, where His78, His81, Glu85, and three HO/OH molecules coordinated to the metal ion. On the other hand, Co-bound and Zn-bound K79H/G80A/H81A Mb exhibited tetrahedral metal-coordination structures, where His79, His82, Asp141, and a HO/OH molecule coordinated to the metal ion. The Co-bound site exists deep inside the protein in the K79H/G80A/H81A Mb dimer, which may allow the unique tetrahedral coordination for the Co ion. These results show that we can utilize domain swapping to construct artificial metalloproteins.
Satoshi Nagao, Ayaka Idomoto, Naoki Shibata, Yoshiki Higuchi, Shun Hirota

1497 related Products with: Rational design of metal-binding sites in domain-swapped myoglobin dimers.

100ul100 ug100.00 ug100 ug100.00 ug10ml100 ul100 μg100ug100 ug96tests

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#33572177   2021/02/09 To Up

Myoglobin Concentration and Oxygen Stores in Different Functional Muscle Groups from Three Small Cetacean Species.

Compared with terrestrial mammals, marine mammals possess increased muscle myoglobin concentrations (Mb concentration, g Mb · 100g muscle), enhancing their onboard oxygen (O) stores and their aerobic dive limit. Although myoglobin is not homogeneously distributed, cetacean muscle O stores have been often determined by measuring Mb concentration from a single muscle sample () and multiplying that value by the animal's locomotor muscle or total muscle mass. This study serves to determine the accuracy of previous cetacean muscle O stores calculations. For that, body muscles from three delphinid species: , , and , were dissected and weighed. Mb concentration was calculated from six muscles/muscle groups (epaxial, hypaxial and ; ; ; and ), each representative of different functional groups (locomotion powering swimming, pectoral fin movement, feeding and respiration, respectively). Results demonstrated that the Mb concentration was heterogeneously distributed, being significantly higher in locomotor muscles. Locomotor muscles were the major contributors to total muscle O stores (mean 92.8%) due to their high Mb concentration and large muscle masses. Compared to this method, previous studies assuming homogenous Mb concentration distribution likely underestimated total muscle O stores by 10% when only considering locomotor muscles and overestimated them by 13% when total muscle mass was considered.
Marina Arregui, Emily M Singleton, Pedro Saavedra, D Ann Pabst, Michael J Moore, Eva Sierra, Miguel A Rivero, Nakita Câmara, Misty Niemeyer, Andreas Fahlman, William A McLellan, Yara Bernaldo de Quirós

2014 related Products with: Myoglobin Concentration and Oxygen Stores in Different Functional Muscle Groups from Three Small Cetacean Species.

50 ug 100ul 100ul1.00 flask50ul 100ul100ug50ul

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#33565187   2021/02/09 To Up

Repeatability of multinuclear interleaved acquisitions with nuclear Overhauser enhancement effect in dynamic experiments in the calf muscle at 3T.

To evaluate the repeatability of multinuclear interleaved H/ P NMR dynamic acquisitions in skeletal muscle and the impact of nuclear Overhauser enhancement (nOe) on the P results at 3T in exercise-recovery and ischemia-hyperemia paradigms.
Alfredo L Lopez Kolkovsky, Benjamin Marty, Eric Giacomini, Martin Meyerspeer, Pierre G Carlier

2525 related Products with: Repeatability of multinuclear interleaved acquisitions with nuclear Overhauser enhancement effect in dynamic experiments in the calf muscle at 3T.

100 μg100ul100 μg96 wells100 µl (2 mM)12 Pieces/Box

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