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#24824853   // To Up

Annexin V imaging detects diabetes-accelerated apoptosis and monitors the efficacy of benfotiamine treatment in ischemic limbs of mice.

The role of apoptosis imaging for monitoring treatment response in ischemic limbs has not been properly explored. In this study, we investigated the ability of annexin V (AnxV) imaging to assess the efficacy of antiapoptotic treatment in ischemic limbs of diabetic mice. Normal C57BL/6 mice and streptozotocin-induced diabetic mice were subject to hindlimb ischemia. AnxV-conjugated fluorescent streptavidin probes were intravenously injected, and optical imaging was performed. Tissue apoptosis was quantified by histochemistry and Western blotting. The AnxV probes showed specific targeting to apoptotic cells on confocal microscopy and flow cytometry. Intravenous AnxV probes displayed substantially greater accumulation in ischemic limbs of diabetic mice. Benfotiamine (BFT) treatment of diabetic mice led to better perfusion recovery on laser Doppler imaging and reduced AnxV binding on optical imaging. TUNEL staining and cleaved caspase-3 Western blots confirmed accelerated apoptosis by diabetes and its suppression by BFT treatment. Furthermore, AnxV-SAv-PEcy5.5 uptake in the ischemic limbs closely correlated to cleaved caspase-3 expression. Thus, AnxV imaging may be useful for monitoring the efficacy of therapeutic agents designed to suppress ischemia-induced apoptosis.
Kyung-Ho Jung, Jin Hee Lee, Jin Won Park, Jin Young Paik, Cung Hoa Thien Quach, Eun Jeong Lee, Kyung-Han Lee

1901 related Products with: Annexin V imaging detects diabetes-accelerated apoptosis and monitors the efficacy of benfotiamine treatment in ischemic limbs of mice.

25 assays100ul100 1 mg

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#24515767   // To Up

A six-color flow cytometry assay for immunophenotyping classical Hodgkin lymphoma in lymph nodes.

We have recently demonstrated that classical Hodgkin lymphoma (CHL) can be immunophenotyped by flow cytometry (FC), thus obviating the need for immunohistochemistry in many cases. The previously described nine-color assay, however, cannot be used by laboratories that do not have access to a nine- or ten-color flow cytometer. Therefore, a six-color FC tube was designed employing the following combination: CD64-FITC/CD30-PE/CD40-PeCy5.5/CD20-PECy7/CD95-APC/CD3-APC-H7.
Jonathan R Fromm, Brent L Wood

1959 related Products with: A six-color flow cytometry assay for immunophenotyping classical Hodgkin lymphoma in lymph nodes.

1 kit1 kit1 kit1 kit1 kit1 kit1 kit

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#23184564   2012/11/26 To Up

Mesenchymal markers on human adipose stem/progenitor cells.

The stromal-vascular fraction (SVF) of adipose tissue is a rich source of multipotent stem cells. We and others have described three major populations of stem/progenitor cells in this fraction, all closely associated with small blood vessels: endothelial progenitor cells (EPC, CD45-/CD31+/CD34+), pericytes (CD45-/CD31-/CD146+), and supra-adventitial adipose stromal cells (SA-ASC, CD45-/CD31-/CD146-/CD34+). EPC are luminal, pericytes are adventitial, and SA-ASC surround the vessel like a sheath. The multipotency of the pericytes and SA-ASC compartments is strikingly similar to that of CD45-/CD34-/CD73+/CD105+/CD90+ bone marrow-derived mesenchymal stem cells (BM-MSC). Here, we determine the extent to which this mesenchymal pattern is expressed on the three adipose stem/progenitor populations. Eight independent adipose tissue samples were analyzed in a single tube (CD105-FITC/CD73-PE/CD146-PETXR/CD14-PECY5/CD33-PECY5/CD235A-PECY5/CD31-PECY7/CD90-APC/CD34-A700/CD45-APCCY7/DAPI). Adipose EPC were highly proliferative with (14.3 ± 2.8)% (mean ± SEM) having >2N DNA. About half (53.1 ± 7.6)% coexpressed CD73 and CD105, and (71.9 ± 7.4)% expressed CD90. Pericytes were less proliferative [(8.2 ± 3.4)% >2N DNA)] with a smaller proportion [(29.6 ± 6.9)% CD73+/CD105+, (60.5 ± 10.2)% CD90+] expressing mesenchymal associated markers. However, the CD34+ subset of CD146+ pericytes were both highly proliferative [(15.1 ± 3.6)% with >2N DNA] and of uniform mesenchymal phenotype [(93.3 ± 3.7)% CD73+/CD105+, (97.8 ± 0.7)% CD90+], suggesting transit amplifying progenitor cells. SA-ASC were the least proliferative [(3.7 ± 0.8)%>2N DNA] but were also highly mesenchymal in phenotype [(94.4 ± 3.2)% CD73+/CD105+, (95.5 ± 1.2)% CD90+]. These data imply a progenitor/progeny relationship between pericytes and SA-ASC, the most mesenchymal of SVF cells. Despite phenotypic and functional similarities to BM-MSC, SA-ASC are distinguished by CD34 expression.
Ludovic Zimmerlin, Vera S Donnenberg, J Peter Rubin, Albert D Donnenberg

1362 related Products with: Mesenchymal markers on human adipose stem/progenitor cells.

1 mg10 ug0.1ml (1mg/ml)5 x 10A5 cells/vial1.00 flask1 mg1.00 flask1 x 10^6 cells/vial225 TESTS1.00 flask200

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#21695775   2011/06/21 To Up

An improved flow cytometric assay for detection and discrimination between malignant cells and atypical mesothelial cells, in serous cavity effusions.

The aim of this study was to evaluate a flow cytometric assay for the detection of malignant effusions.
Nektaria A Kentrou, Nikolaos J Tsagarakis, Konstantina Tzanetou, Maria Damala, Konstantinos A Papadimitriou, Dimitra Skoumi, Aimilia Stratigaki, Nikolaos I Anagnostopoulos, Eleni Malamou-Lada, Pauline Athanassiadou, George Paterakis

2419 related Products with: An improved flow cytometric assay for detection and discrimination between malignant cells and atypical mesothelial cells, in serous cavity effusions.

100 µg100 µg1 mg2 ml96 assays1 g96 wells (1 kit)1 mg100ul25 assays

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#21537148   // To Up

Minimal residual disease is a prognostic marker for neuroblastoma with bone marrow infiltration.

This pilot study focused on whether flow cytometry (FCM) detection of minimal residual disease in bone marrow (BM) could predict the outcome of patients with advanced neuroblastoma (NB).
Jiao-Yang Cai, Ci Pan, Yan-Jing Tang, Jing Chen, Qi-Dong Ye, Min Zhou, Huiliang Xue, Jing-Yan Tang

2982 related Products with: Minimal residual disease is a prognostic marker for neuroblastoma with bone marrow infiltration.

0.25 mg100ug Lyophilized1 ml100ug Lyophilized100ug Lyophilized100ug Lyophilized1 LITRE100ug Lyophilized100ug Lyophilized

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#18823984   2008/09/25 To Up

Oxidative burst assessment and neutrophil-platelet complexes in unlysed whole blood.

Methods currently employed for measuring reactive oxygen species production can lead to both cellular depletion and in artifactual activation. The objective of this study was to design a methodology allowing the measurement of oxidative burst (OB) with minimal sample manipulation.
Ariadna Avendaño, Irene Sales-Pardo, Lorena Marin, Pedro Marin, Jordi Petriz

2824 related Products with: Oxidative burst assessment and neutrophil-platelet complexes in unlysed whole blood.

100 UG100 assays96 testsUnit100 mg100 20 10mg1mg100 500mg

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#18683684   // To Up

[Impacts of microparticles on vascular endothelial cells].

To investigate the effects of microparticles (Mps) from different people on the vascular endothelial cell function.
Yi Wei, Zhi-hong Wu, Gui-xing Qiu, Cun-ji Gao, Xi-sheng Weng

2668 related Products with: [Impacts of microparticles on vascular endothelial cells].

1.00 flask1.00 flask2ug1.00 flask0.1 mg1.00 flask1.00 flask2ug1.00 flask1.00 flask2ug x 201.00 flask

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#16241954   // To Up

Effects of platelet binding on whole blood flow cytometry assays of monocyte and neutrophil procoagulant activity.

Monocytes and neutrophils form heterotypic aggregates with platelets initially via engagement of platelet surface P-selectin with leukocyte surface P-selectin glycoprotein ligand-1 (PSGL-1). The resultant intracellular signaling causes the leukocyte surface expression of tissue factor and activation of leukocyte surface Mac-1 (integrin alphaMbeta2, CD11b/CD18). The activation-dependent conformational change in monocyte surface Mac-1 results in the binding of coagulation factor Xa (FXa) and/or fibrinogen to Mac-1. The aim of this study was to develop whole blood flow cytometry assays of these procoagulant activities and to investigate the effects of platelet binding to monocytes and neutrophils.
M R Barnard, M D Linden, A L Frelinger, Y Li, M L Fox, M I Furman, A D Michelson

1915 related Products with: Effects of platelet binding on whole blood flow cytometry assays of monocyte and neutrophil procoagulant activity.

1 kit1 kit1 kit1 kit1 kit1 kit48 assays1 kit1 kit100 assays1 kit

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